Moraxellaceae bacterium HYN0046 
Average proteome isoelectric point is 6.51 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 3297 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A345P682|A0A345P682_9GAMM Rhodanese domain-containing protein OS=Moraxellaceae bacterium HYN0046 OX=2283318 GN=HYN46_08055 PE=4 SV=1 
MM1 pKa = 7.7  KK2 pKa = 10.11  KK3 pKa = 9.69  YY4 pKa = 10.65  QCIVCGWIYY13 pKa = 11.06  DD14 pKa = 4.1  EE15 pKa = 5.82  AIGWPDD21 pKa = 3.5  DD22 pKa = 4.45  GIPAGTLWDD31 pKa = 5.92  DD32 pKa = 4.69  IPDD35 pKa = 3.34  SWVCPDD41 pKa = 4.2  CGVGKK46 pKa = 10.5  ADD48 pKa = 3.78  FEE50 pKa = 4.63  MIEE53 pKa = 4.07  VV54 pKa = 3.62   
 Molecular weight: 6.05 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.82 
IPC2_protein 3.681 
IPC_protein 3.592 
Toseland    3.389 
ProMoST     3.821 
Dawson      3.617 
Bjellqvist  3.783 
Wikipedia   3.617 
Rodwell     3.439 
Grimsley    3.312 
Solomon     3.579 
Lehninger   3.541 
Nozaki      3.77 
DTASelect   3.999 
Thurlkill   3.49 
EMBOSS      3.617 
Sillero     3.732 
Patrickios  0.299 
IPC_peptide 3.567 
IPC2_peptide  3.694 
IPC2.peptide.svr19  3.697 
 Protein with the highest isoelectric point: 
>tr|A0A345PAM8|A0A345PAM8_9GAMM Ribosome maturation factor RimP OS=Moraxellaceae bacterium HYN0046 OX=2283318 GN=rimP PE=3 SV=1 
MM1 pKa = 7.45  KK2 pKa = 9.53  RR3 pKa = 11.84  TFQPSSLKK11 pKa = 10.08  RR12 pKa = 11.84  KK13 pKa = 8.62  RR14 pKa = 11.84  VHH16 pKa = 6.43  GFRR19 pKa = 11.84  ARR21 pKa = 11.84  MATKK25 pKa = 10.35  NGRR28 pKa = 11.84  LVLARR33 pKa = 11.84  RR34 pKa = 11.84  RR35 pKa = 11.84  AKK37 pKa = 10.22  GRR39 pKa = 11.84  ARR41 pKa = 11.84  LTVV44 pKa = 3.04   
 Molecular weight: 5.16 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.523 
IPC2_protein 11.242 
IPC_protein 12.837 
Toseland    13.013 
ProMoST     13.495 
Dawson      13.013 
Bjellqvist  12.998 
Wikipedia   13.481 
Rodwell     12.676 
Grimsley    13.042 
Solomon     13.495 
Lehninger   13.408 
Nozaki      13.013 
DTASelect   12.998 
Thurlkill   13.013 
EMBOSS      13.51 
Sillero     13.013 
Patrickios  12.398 
IPC_peptide 13.51 
IPC2_peptide  12.486 
IPC2.peptide.svr19  9.177 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        3297 
 
        
        0
 
        
        3297 
         
        1068716
 
        22
 
        2422
 
        324.1
 
        35.7
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        9.69 ± 0.043
0.823 ± 0.014
 
        5.331 ± 0.035
5.095 ± 0.047
 
        3.938 ± 0.031
7.152 ± 0.04
 
        2.439 ± 0.026
6.262 ± 0.036
       
        4.471 ± 0.04
10.574 ± 0.057
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.365 ± 0.021
3.908 ± 0.034
 
        4.57 ± 0.027
4.626 ± 0.034
 
        5.016 ± 0.036
6.577 ± 0.076
 
        5.943 ± 0.04
7.014 ± 0.036
       
        1.32 ± 0.017
2.887 ± 0.031
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here