Moraxellaceae bacterium HYN0046
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3297 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345P682|A0A345P682_9GAMM Rhodanese domain-containing protein OS=Moraxellaceae bacterium HYN0046 OX=2283318 GN=HYN46_08055 PE=4 SV=1
MM1 pKa = 7.7 KK2 pKa = 10.11 KK3 pKa = 9.69 YY4 pKa = 10.65 QCIVCGWIYY13 pKa = 11.06 DD14 pKa = 4.1 EE15 pKa = 5.82 AIGWPDD21 pKa = 3.5 DD22 pKa = 4.45 GIPAGTLWDD31 pKa = 5.92 DD32 pKa = 4.69 IPDD35 pKa = 3.34 SWVCPDD41 pKa = 4.2 CGVGKK46 pKa = 10.5 ADD48 pKa = 3.78 FEE50 pKa = 4.63 MIEE53 pKa = 4.07 VV54 pKa = 3.62
Molecular weight: 6.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.82
IPC2_protein 3.681
IPC_protein 3.592
Toseland 3.389
ProMoST 3.821
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.617
Rodwell 3.439
Grimsley 3.312
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.999
Thurlkill 3.49
EMBOSS 3.617
Sillero 3.732
Patrickios 0.299
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.697
Protein with the highest isoelectric point:
>tr|A0A345PAM8|A0A345PAM8_9GAMM Ribosome maturation factor RimP OS=Moraxellaceae bacterium HYN0046 OX=2283318 GN=rimP PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.53 RR3 pKa = 11.84 TFQPSSLKK11 pKa = 10.08 RR12 pKa = 11.84 KK13 pKa = 8.62 RR14 pKa = 11.84 VHH16 pKa = 6.43 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.35 NGRR28 pKa = 11.84 LVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVV44 pKa = 3.04
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3297
0
3297
1068716
22
2422
324.1
35.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.69 ± 0.043
0.823 ± 0.014
5.331 ± 0.035
5.095 ± 0.047
3.938 ± 0.031
7.152 ± 0.04
2.439 ± 0.026
6.262 ± 0.036
4.471 ± 0.04
10.574 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.365 ± 0.021
3.908 ± 0.034
4.57 ± 0.027
4.626 ± 0.034
5.016 ± 0.036
6.577 ± 0.076
5.943 ± 0.04
7.014 ± 0.036
1.32 ± 0.017
2.887 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here