Streptococcus satellite phage Javan160
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZFZ4|A0A4D5ZFZ4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan160 OX=2558549 GN=JavanS160_0009 PE=4 SV=1
MM1 pKa = 7.89 PEE3 pKa = 4.21 FIIVEE8 pKa = 4.28 GNNDD12 pKa = 3.55 LGEE15 pKa = 4.16 FFQVDD20 pKa = 3.89 GEE22 pKa = 4.42 LFSDD26 pKa = 4.66 VEE28 pKa = 4.26 LLGNLKK34 pKa = 10.09 KK35 pKa = 9.8 WDD37 pKa = 3.71 EE38 pKa = 4.18 WDD40 pKa = 3.31 VSIIIDD46 pKa = 4.15 DD47 pKa = 3.74 DD48 pKa = 4.17 TNRR51 pKa = 11.84 SISDD55 pKa = 3.67 DD56 pKa = 3.62 FSEE59 pKa = 4.71 IIYY62 pKa = 10.43 FPTHH66 pKa = 6.76 EE67 pKa = 5.34 DD68 pKa = 3.06 NMDD71 pKa = 3.94 YY72 pKa = 10.91 IRR74 pKa = 11.84 VNKK77 pKa = 9.93 GLEE80 pKa = 4.08 PLYY83 pKa = 9.8 HH84 pKa = 7.1 TINKK88 pKa = 9.51 PYY90 pKa = 8.92 VTISKK95 pKa = 10.3 SEE97 pKa = 3.86 WLEE100 pKa = 3.82 LLDD103 pKa = 4.57
Molecular weight: 12.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.184
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.681
ProMoST 3.973
Dawson 3.859
Bjellqvist 4.062
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.808
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.795
Sillero 3.999
Patrickios 3.478
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.933
Protein with the highest isoelectric point:
>tr|A0A4D5ZEZ0|A0A4D5ZEZ0_9VIRU Putative transcription regulator OS=Streptococcus satellite phage Javan160 OX=2558549 GN=JavanS160_0013 PE=4 SV=1
MM1 pKa = 7.93 LITLSLAEE9 pKa = 3.99 KK10 pKa = 10.58 VRR12 pKa = 11.84 IKK14 pKa = 10.5 RR15 pKa = 11.84 ARR17 pKa = 11.84 LQLTKK22 pKa = 10.44 KK23 pKa = 10.22 AASEE27 pKa = 3.99 QIGIQSNTLTKK38 pKa = 10.5 VEE40 pKa = 4.33 NGNYY44 pKa = 8.14 DD45 pKa = 3.37 APKK48 pKa = 10.04 RR49 pKa = 11.84 IYY51 pKa = 10.18 EE52 pKa = 4.36 SVMTWLVEE60 pKa = 4.75 KK61 pKa = 10.57 KK62 pKa = 10.5 FKK64 pKa = 10.72 SNKK67 pKa = 7.37 KK68 pKa = 9.49 AVYY71 pKa = 9.41 RR72 pKa = 11.84 RR73 pKa = 11.84 PNQRR77 pKa = 11.84 IRR79 pKa = 11.84 LRR81 pKa = 11.84 KK82 pKa = 9.03 NNKK85 pKa = 6.78 TQAKK89 pKa = 8.3 ARR91 pKa = 11.84 RR92 pKa = 11.84 VVLLGII98 pKa = 4.39
Molecular weight: 11.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.232
IPC2_protein 10.189
IPC_protein 11.199
Toseland 11.433
ProMoST 11.608
Dawson 11.462
Bjellqvist 11.301
Wikipedia 11.798
Rodwell 11.681
Grimsley 11.506
Solomon 11.784
Lehninger 11.711
Nozaki 11.418
DTASelect 11.301
Thurlkill 11.418
EMBOSS 11.886
Sillero 11.433
Patrickios 11.403
IPC_peptide 11.784
IPC2_peptide 10.57
IPC2.peptide.svr19 8.621
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2382
55
401
158.8
18.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.584 ± 0.606
0.42 ± 0.108
5.835 ± 0.652
7.389 ± 0.772
3.652 ± 0.384
4.114 ± 0.297
1.721 ± 0.264
8.312 ± 1.093
9.614 ± 0.783
9.95 ± 0.484
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.519 ± 0.291
6.087 ± 0.474
2.351 ± 0.205
4.618 ± 0.577
4.24 ± 0.497
7.137 ± 0.662
5.29 ± 0.642
6.045 ± 0.591
1.05 ± 0.205
4.072 ± 0.445
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here