Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanomicrobiales; Methanospirillaceae; Methanospirillum; Methanospirillum hungatei

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3087 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q2FS66|SYR_METHJ Arginine--tRNA ligase OS=Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1) OX=323259 GN=argS PE=3 SV=1
MM1 pKa = 7.43RR2 pKa = 11.84AAHH5 pKa = 6.56IFVLLLLLWITPAGMGEE22 pKa = 4.08EE23 pKa = 4.33TQDD26 pKa = 4.92DD27 pKa = 3.77ISPAQTHH34 pKa = 6.08NLLVIYY40 pKa = 8.28MIAGDD45 pKa = 5.22LEE47 pKa = 4.69TEE49 pKa = 4.38TQSATMNIAEE59 pKa = 4.64ILDD62 pKa = 4.55GYY64 pKa = 11.14GKK66 pKa = 9.05TSEE69 pKa = 4.13QNLQIVLGYY78 pKa = 10.23GGSKK82 pKa = 9.15TPGFGGITYY91 pKa = 7.72VTVKK95 pKa = 10.65EE96 pKa = 4.07LMEE99 pKa = 4.81DD100 pKa = 3.36ASDD103 pKa = 3.85GVIGNTEE110 pKa = 3.93DD111 pKa = 4.14VLYY114 pKa = 10.32HH115 pKa = 7.46DD116 pKa = 5.86PDD118 pKa = 5.31ADD120 pKa = 3.68MGDD123 pKa = 3.83KK124 pKa = 9.67KK125 pKa = 9.47TLEE128 pKa = 4.85HH129 pKa = 5.87FLSWIGEE136 pKa = 4.17NFPSDD141 pKa = 3.33RR142 pKa = 11.84KK143 pKa = 10.42ILVFWDD149 pKa = 3.13HH150 pKa = 6.48GGGYY154 pKa = 10.35DD155 pKa = 3.74GFGVDD160 pKa = 4.86EE161 pKa = 4.42VTDD164 pKa = 4.3GQLSLTDD171 pKa = 3.43ISQALEE177 pKa = 4.19ASGSTYY183 pKa = 11.4DD184 pKa = 4.72LIGYY188 pKa = 7.57DD189 pKa = 3.76ACLMGALEE197 pKa = 4.29VAKK200 pKa = 10.79AMEE203 pKa = 4.84PYY205 pKa = 10.38GILLIGSEE213 pKa = 4.27EE214 pKa = 4.34TEE216 pKa = 4.46PGTGWEE222 pKa = 4.13YY223 pKa = 9.81EE224 pKa = 4.15TWIKK228 pKa = 11.03ALGEE232 pKa = 3.91NPEE235 pKa = 4.08IGFEE239 pKa = 4.01EE240 pKa = 4.61LGQIIVDD247 pKa = 3.94TYY249 pKa = 10.51MNRR252 pKa = 11.84DD253 pKa = 3.58DD254 pKa = 3.73TGKK257 pKa = 7.73TLSVIDD263 pKa = 3.91LTKK266 pKa = 10.38IPALIQALDD275 pKa = 3.61RR276 pKa = 11.84LGTSMMPYY284 pKa = 8.58TEE286 pKa = 5.01SIEE289 pKa = 4.33GYY291 pKa = 9.35RR292 pKa = 11.84VVGKK296 pKa = 10.35AYY298 pKa = 9.66QVPARR303 pKa = 11.84YY304 pKa = 9.77GSDD307 pKa = 2.85NRR309 pKa = 11.84EE310 pKa = 4.02GGEE313 pKa = 3.97TSVDD317 pKa = 3.22LKK319 pKa = 11.45SFLKK323 pKa = 10.91AIGNQTPDD331 pKa = 3.59LADD334 pKa = 4.68EE335 pKa = 4.39IAVVTTRR342 pKa = 11.84IDD344 pKa = 3.41EE345 pKa = 4.34TVLYY349 pKa = 9.81HH350 pKa = 7.5RR351 pKa = 11.84NDD353 pKa = 3.25EE354 pKa = 4.3YY355 pKa = 11.66VPEE358 pKa = 4.64SGGLSIMSPSRR369 pKa = 11.84ITPDD373 pKa = 3.0LYY375 pKa = 11.35EE376 pKa = 4.44EE377 pKa = 4.98LGDD380 pKa = 4.47DD381 pKa = 5.05ARR383 pKa = 11.84ITPGWDD389 pKa = 2.98SFFVNLLEE397 pKa = 5.49ISGQDD402 pKa = 3.4TEE404 pKa = 4.75KK405 pKa = 10.92PEE407 pKa = 3.99IMRR410 pKa = 11.84SDD412 pKa = 3.36SGFLIEE418 pKa = 5.99DD419 pKa = 3.76PTNTASVYY427 pKa = 10.79AEE429 pKa = 4.17YY430 pKa = 10.9YY431 pKa = 9.52YY432 pKa = 11.49ADD434 pKa = 3.95GDD436 pKa = 4.06EE437 pKa = 5.81LILLGNEE444 pKa = 4.13PLDD447 pKa = 3.98PDD449 pKa = 3.77EE450 pKa = 4.84NGEE453 pKa = 4.2YY454 pKa = 9.94TLPEE458 pKa = 3.75WDD460 pKa = 3.59GRR462 pKa = 11.84WYY464 pKa = 10.86YY465 pKa = 10.17LQDD468 pKa = 3.47MNNEE472 pKa = 4.0DD473 pKa = 3.93NYY475 pKa = 11.86ALLGMNFEE483 pKa = 5.19SIGPSGSVLYY493 pKa = 10.16TSEE496 pKa = 3.77IDD498 pKa = 5.08LIRR501 pKa = 11.84EE502 pKa = 4.28SLNTTAVLNVYY513 pKa = 10.23INPQTGEE520 pKa = 4.1TRR522 pKa = 11.84LVACPYY528 pKa = 9.32TIRR531 pKa = 11.84PNGIIQFSRR540 pKa = 11.84QNLDD544 pKa = 3.64LEE546 pKa = 4.98PNDD549 pKa = 3.89TVYY552 pKa = 11.04SYY554 pKa = 11.22AWKK557 pKa = 10.34VDD559 pKa = 3.0EE560 pKa = 4.45DD561 pKa = 4.68TEE563 pKa = 4.68TGGEE567 pKa = 4.02WVEE570 pKa = 4.17IGALVISGNTKK581 pKa = 10.51LIYY584 pKa = 10.34DD585 pKa = 4.41ILPDD589 pKa = 3.51GTYY592 pKa = 10.82AQALYY597 pKa = 10.86AEE599 pKa = 4.95YY600 pKa = 10.92GNKK603 pKa = 9.81PGDD606 pKa = 3.63YY607 pKa = 10.93AGMQVFQIEE616 pKa = 4.34NGEE619 pKa = 3.97ITLVEE624 pKa = 4.31SEE626 pKa = 4.58TNITALQEE634 pKa = 4.15PEE636 pKa = 4.51EE637 pKa = 4.28NNQQ640 pKa = 3.05

Molecular weight: 70.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2FTM5|Q2FTM5_METHJ Oligosaccharyl transferase OS=Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1) OX=323259 GN=Mhun_2859 PE=3 SV=1
MM1 pKa = 7.72SKK3 pKa = 8.9LTKK6 pKa = 9.67CRR8 pKa = 11.84KK9 pKa = 8.99IRR11 pKa = 11.84LSKK14 pKa = 9.95KK15 pKa = 5.62TTQNRR20 pKa = 11.84RR21 pKa = 11.84VPQWVMVKK29 pKa = 7.88TARR32 pKa = 11.84AVMAHH37 pKa = 6.04PQRR40 pKa = 11.84HH41 pKa = 4.16SWRR44 pKa = 11.84RR45 pKa = 11.84STLKK49 pKa = 10.66VV50 pKa = 3.07

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3087

0

3087

996237

41

2353

322.7

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.034 ± 0.047

1.366 ± 0.022

5.781 ± 0.033

6.755 ± 0.056

3.981 ± 0.034

7.352 ± 0.042

2.202 ± 0.021

8.521 ± 0.051

4.99 ± 0.041

9.18 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.02

3.579 ± 0.033

4.686 ± 0.041

3.185 ± 0.025

5.179 ± 0.039

6.687 ± 0.043

5.803 ± 0.049

6.584 ± 0.037

1.06 ± 0.017

3.438 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski