Choristoneura rosaceana entomopoxvirus L
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 286 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4ZDY2|R4ZDY2_9POXV N1R/p28-like protein OS=Choristoneura rosaceana entomopoxvirus 'L' OX=1293539 GN=CHREV_138 PE=4 SV=1
MM1 pKa = 7.67 TDD3 pKa = 2.74 STTEE7 pKa = 3.4 IMEE10 pKa = 4.31 FDD12 pKa = 4.12 VEE14 pKa = 4.36 EE15 pKa = 4.68 NDD17 pKa = 3.69 DD18 pKa = 4.18 KK19 pKa = 11.74 YY20 pKa = 11.75 VIAIDD25 pKa = 4.18 TNIQNDD31 pKa = 3.5 NDD33 pKa = 4.12 DD34 pKa = 4.53 NIMMEE39 pKa = 4.25 RR40 pKa = 11.84 EE41 pKa = 3.5 NVYY44 pKa = 10.85 YY45 pKa = 8.5 YY46 pKa = 10.52 TNHH49 pKa = 5.7 EE50 pKa = 4.25 NKK52 pKa = 10.14 VIII55 pKa = 4.51
Molecular weight: 6.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.745
IPC_protein 3.668
Toseland 3.478
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.389
Solomon 3.63
Lehninger 3.592
Nozaki 3.795
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.617
Sillero 3.795
Patrickios 1.85
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|R4ZEA1|R4ZEA1_9POXV Uncharacterized protein OS=Choristoneura rosaceana entomopoxvirus 'L' OX=1293539 GN=CHREV_250 PE=4 SV=1
MM1 pKa = 7.63 NIFGLIVSSLALISSIIVVIFNIFRR26 pKa = 11.84 LRR28 pKa = 11.84 GLLLTKK34 pKa = 10.66 LILYY38 pKa = 8.52 VILVGSIIIIIFIFTT53 pKa = 3.6
Molecular weight: 5.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.282
IPC2_protein 10.248
IPC_protein 11.155
Toseland 11.096
ProMoST 11.155
Dawson 11.169
Bjellqvist 10.994
Wikipedia 11.491
Rodwell 11.242
Grimsley 11.228
Solomon 11.447
Lehninger 11.389
Nozaki 11.067
DTASelect 11.008
Thurlkill 11.096
EMBOSS 11.535
Sillero 11.125
Patrickios 11.199
IPC_peptide 11.447
IPC2_peptide 10.204
IPC2.peptide.svr19 8.012
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
286
0
286
79222
49
1300
277.0
32.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.747 ± 0.077
1.829 ± 0.081
6.542 ± 0.106
5.124 ± 0.125
4.673 ± 0.097
2.296 ± 0.111
1.511 ± 0.047
14.536 ± 0.219
9.977 ± 0.208
8.674 ± 0.129
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.861 ± 0.065
12.874 ± 0.224
2.071 ± 0.08
1.944 ± 0.061
2.422 ± 0.095
5.865 ± 0.114
4.516 ± 0.095
3.321 ± 0.101
0.587 ± 0.044
7.629 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here