Rhodococcus aetherivorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5509 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059MHW6|A0A059MHW6_9NOCA Peptidase M13 OS=Rhodococcus aetherivorans OX=191292 GN=N505_0120845 PE=4 SV=1
MM1 pKa = 7.17NFIRR5 pKa = 11.84RR6 pKa = 11.84VSGTAVALAAAALVLGACGSDD27 pKa = 3.24VEE29 pKa = 4.47PRR31 pKa = 11.84AVPEE35 pKa = 4.46SGSEE39 pKa = 4.1TTSASASDD47 pKa = 3.27TRR49 pKa = 11.84TTTGRR54 pKa = 11.84PTTTTAPTSPGSSGLDD70 pKa = 2.76IDD72 pKa = 5.41VEE74 pKa = 4.2IGDD77 pKa = 4.46CVRR80 pKa = 11.84LGGTVDD86 pKa = 4.2DD87 pKa = 4.42ATIEE91 pKa = 4.09NATCGSMDD99 pKa = 3.67SNYY102 pKa = 10.56KK103 pKa = 10.63VIDD106 pKa = 3.54IVDD109 pKa = 3.64SSEE112 pKa = 4.1QCVSDD117 pKa = 4.42ADD119 pKa = 3.5QTYY122 pKa = 8.17YY123 pKa = 10.79EE124 pKa = 4.38SSAGVEE130 pKa = 4.2VGALCLDD137 pKa = 3.79VDD139 pKa = 4.21WVVDD143 pKa = 3.59SCMDD147 pKa = 3.4ISGEE151 pKa = 3.89DD152 pKa = 3.83PQRR155 pKa = 11.84VDD157 pKa = 3.64CAAPGPEE164 pKa = 4.17TEE166 pKa = 4.92KK167 pKa = 10.15VTEE170 pKa = 3.94ILQNTDD176 pKa = 2.8NVNDD180 pKa = 3.92CSVSEE185 pKa = 4.12GGFVYY190 pKa = 9.84SEE192 pKa = 3.9RR193 pKa = 11.84RR194 pKa = 11.84FVVCTDD200 pKa = 3.38TLL202 pKa = 3.7

Molecular weight: 20.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059MKB0|A0A059MKB0_9NOCA GABA permease OS=Rhodococcus aetherivorans OX=191292 GN=N505_0124105 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 10.08GRR42 pKa = 11.84TSLTAA47 pKa = 3.9

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5509

0

5509

1815698

32

7327

329.6

35.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.479 ± 0.054

0.758 ± 0.01

6.287 ± 0.026

5.652 ± 0.028

2.948 ± 0.019

9.126 ± 0.028

2.264 ± 0.015

3.724 ± 0.021

1.774 ± 0.021

10.19 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.831 ± 0.014

1.797 ± 0.015

5.859 ± 0.03

2.579 ± 0.018

7.948 ± 0.035

4.914 ± 0.023

6.192 ± 0.021

9.212 ± 0.035

1.449 ± 0.015

2.017 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski