Po-Circo-like virus 41
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8E3Y2|G8E3Y2_9CIRC Uncharacterized protein OS=Po-Circo-like virus 41 OX=1105385 PE=4 SV=1
MM1 pKa = 7.21 SRR3 pKa = 11.84 IRR5 pKa = 11.84 GTRR8 pKa = 11.84 WCFTINNFTQEE19 pKa = 3.97 EE20 pKa = 4.59 EE21 pKa = 4.23 EE22 pKa = 4.67 LVMNMPSTGWVKK34 pKa = 11.3 SMIAEE39 pKa = 4.11 EE40 pKa = 3.94 EE41 pKa = 4.27 HH42 pKa = 7.39 LFEE45 pKa = 4.66 GTPHH49 pKa = 5.35 IQGYY53 pKa = 7.13 FTTTKK58 pKa = 9.76 RR59 pKa = 11.84 TDD61 pKa = 3.55 FTALTKK67 pKa = 10.19 FFNGRR72 pKa = 11.84 AHH74 pKa = 7.45 LEE76 pKa = 3.88 QAKK79 pKa = 10.58 GSTRR83 pKa = 11.84 DD84 pKa = 2.9 NWNYY88 pKa = 9.98 CSKK91 pKa = 9.81 EE92 pKa = 3.74 GNVIVEE98 pKa = 4.73 FNRR101 pKa = 11.84 PSTVDD106 pKa = 3.48 RR107 pKa = 11.84 VPHH110 pKa = 6.63 RR111 pKa = 11.84 AHH113 pKa = 7.43 EE114 pKa = 4.36 DD115 pKa = 3.71 PKK117 pKa = 11.51 DD118 pKa = 3.34 EE119 pKa = 4.39 WADD122 pKa = 4.76 LMIQDD127 pKa = 4.9 CEE129 pKa = 4.29 QMDD132 pKa = 3.84 RR133 pKa = 11.84 EE134 pKa = 4.71 TFRR137 pKa = 11.84 SEE139 pKa = 3.96 HH140 pKa = 6.63 PNFFLNQRR148 pKa = 11.84 DD149 pKa = 3.75 KK150 pKa = 11.6 YY151 pKa = 10.36 EE152 pKa = 4.72 RR153 pKa = 11.84 IHH155 pKa = 7.16 NEE157 pKa = 3.34 YY158 pKa = 10.2 LARR161 pKa = 11.84 TTTVFDD167 pKa = 4.51 GRR169 pKa = 11.84 LHH171 pKa = 7.42 DD172 pKa = 4.25 KK173 pKa = 10.79 NIWIYY178 pKa = 10.8 GPPGSGKK185 pKa = 8.61 STLARR190 pKa = 11.84 SDD192 pKa = 3.45 TPIDD196 pKa = 3.87 HH197 pKa = 7.38 IYY199 pKa = 10.48 FKK201 pKa = 10.78 PVDD204 pKa = 3.66 KK205 pKa = 10.17 WWDD208 pKa = 3.37 GFDD211 pKa = 3.55 PSKK214 pKa = 10.86 HH215 pKa = 5.28 NRR217 pKa = 11.84 IVMDD221 pKa = 4.89 DD222 pKa = 4.06 YY223 pKa = 11.57 PNEE226 pKa = 4.12 ATGGAIFTQRR236 pKa = 11.84 MKK238 pKa = 10.35 IWGDD242 pKa = 2.94 RR243 pKa = 11.84 TTEE246 pKa = 3.76 PRR248 pKa = 11.84 PVKK251 pKa = 10.53 GGTITVDD258 pKa = 3.2 PNIPFIVTSNFPIDD272 pKa = 3.42 EE273 pKa = 4.47 CFSNDD278 pKa = 2.85 VDD280 pKa = 3.75 RR281 pKa = 11.84 EE282 pKa = 4.28 AIHH285 pKa = 7.16 RR286 pKa = 11.84 RR287 pKa = 11.84 FQEE290 pKa = 3.78 IYY292 pKa = 10.97 LDD294 pKa = 3.97 GDD296 pKa = 3.32 KK297 pKa = 11.37 SKK299 pKa = 11.27 LDD301 pKa = 3.21 RR302 pKa = 11.84 FMHH305 pKa = 6.42 LKK307 pKa = 10.61 NLINPEE313 pKa = 4.48 DD314 pKa = 4.63 PLDD317 pKa = 4.58 CDD319 pKa = 4.6 DD320 pKa = 6.25 SIVSDD325 pKa = 3.69
Molecular weight: 38.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.221
IPC2_protein 5.181
IPC_protein 5.169
Toseland 5.258
ProMoST 5.296
Dawson 5.245
Bjellqvist 5.347
Wikipedia 5.169
Rodwell 5.169
Grimsley 5.232
Solomon 5.245
Lehninger 5.207
Nozaki 5.41
DTASelect 5.588
Thurlkill 5.308
EMBOSS 5.283
Sillero 5.474
Patrickios 4.228
IPC_peptide 5.258
IPC2_peptide 5.474
IPC2.peptide.svr19 5.443
Protein with the highest isoelectric point:
>tr|G8E3Y3|G8E3Y3_9CIRC Uncharacterized protein OS=Po-Circo-like virus 41 OX=1105385 PE=4 SV=1
MM1 pKa = 7.61 FGICNRR7 pKa = 11.84 GSIIFLMVYY16 pKa = 9.1 RR17 pKa = 11.84 RR18 pKa = 11.84 SYY20 pKa = 8.99 RR21 pKa = 11.84 RR22 pKa = 11.84 SYY24 pKa = 9.49 SRR26 pKa = 11.84 SYY28 pKa = 10.43 SRR30 pKa = 11.84 PVQPVRR36 pKa = 11.84 YY37 pKa = 9.88 SNEE40 pKa = 3.99 TYY42 pKa = 10.95 NVTFQYY48 pKa = 10.8 QWTATPTSATVTMIPASEE66 pKa = 4.12 VQGLRR71 pKa = 11.84 KK72 pKa = 8.21 TKK74 pKa = 10.68 NFTLNISQSPTTNAQGVVQSTSSFIYY100 pKa = 10.34 ALVYY104 pKa = 10.58 VPAGTVAGAITIGNGNAAASLYY126 pKa = 10.35 EE127 pKa = 4.05 PNQNVICSGVCDD139 pKa = 3.84 SSSGQLRR146 pKa = 11.84 ISSRR150 pKa = 11.84 LARR153 pKa = 11.84 NLNSGDD159 pKa = 3.49 SVALVLRR166 pKa = 11.84 PTYY169 pKa = 8.1 TTGTEE174 pKa = 4.24 GNITRR179 pKa = 11.84 ASVTLNFAIAYY190 pKa = 8.25
Molecular weight: 20.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.5
IPC2_protein 9.853
IPC_protein 10.467
Toseland 9.589
ProMoST 9.75
Dawson 10.014
Bjellqvist 9.897
Wikipedia 10.335
Rodwell 10.014
Grimsley 10.175
Solomon 10.058
Lehninger 9.984
Nozaki 9.706
DTASelect 9.882
Thurlkill 9.794
EMBOSS 10.058
Sillero 9.955
Patrickios 3.808
IPC_peptide 10.043
IPC2_peptide 9.18
IPC2.peptide.svr19 8.339
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
706
190
325
235.3
26.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.674 ± 1.343
1.7 ± 0.145
5.807 ± 2.37
4.958 ± 1.518
4.958 ± 0.798
6.799 ± 0.879
2.125 ± 1.011
6.799 ± 0.209
3.966 ± 1.08
5.524 ± 0.808
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.125 ± 0.394
6.799 ± 0.591
5.524 ± 0.47
3.399 ± 0.614
5.807 ± 1.533
8.782 ± 2.369
8.074 ± 0.982
5.949 ± 1.096
1.275 ± 0.733
4.958 ± 1.557
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here