Beihai sobemo-like virus 23

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KEA7|A0A1L3KEA7_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 23 OX=1922695 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 5.85DD3 pKa = 3.27AAKK6 pKa = 10.18RR7 pKa = 11.84QSLCYY12 pKa = 9.58HH13 pKa = 6.38GRR15 pKa = 11.84RR16 pKa = 11.84NYY18 pKa = 8.8QVSMNSIHH26 pKa = 7.06PNDD29 pKa = 3.63SLEE32 pKa = 4.03IVDD35 pKa = 4.48ILEE38 pKa = 4.04EE39 pKa = 4.7AYY41 pKa = 8.85TKK43 pKa = 10.77CRR45 pKa = 11.84FKK47 pKa = 11.1VPKK50 pKa = 10.43FNFSWFYY57 pKa = 11.48NEE59 pKa = 3.87VLHH62 pKa = 5.77TVEE65 pKa = 5.4FSSSPGYY72 pKa = 9.82PMNEE76 pKa = 3.99KK77 pKa = 10.69YY78 pKa = 9.17STNAEE83 pKa = 3.95LFGWDD88 pKa = 4.36GEE90 pKa = 4.27NLDD93 pKa = 3.38VDD95 pKa = 4.11KK96 pKa = 11.37VYY98 pKa = 10.91EE99 pKa = 4.14VYY101 pKa = 10.79KK102 pKa = 10.49KK103 pKa = 10.77VKK105 pKa = 9.59QRR107 pKa = 11.84LINLLHH113 pKa = 6.56GPEE116 pKa = 4.67ADD118 pKa = 4.81PINLFIKK125 pKa = 10.4DD126 pKa = 3.89EE127 pKa = 3.99PHH129 pKa = 6.46KK130 pKa = 10.61KK131 pKa = 10.15SKK133 pKa = 10.98EE134 pKa = 3.87EE135 pKa = 3.6VGAWRR140 pKa = 11.84LISGASIIDD149 pKa = 3.68VMVDD153 pKa = 3.34RR154 pKa = 11.84YY155 pKa = 11.19LFGTLSDD162 pKa = 3.51MMINKK167 pKa = 10.04CYY169 pKa = 10.31DD170 pKa = 3.27IPNKK174 pKa = 10.53AGWSPVSGGYY184 pKa = 9.92KK185 pKa = 9.34WMYY188 pKa = 10.33SVLGNSLKK196 pKa = 10.46MMADD200 pKa = 3.1KK201 pKa = 11.13SSWDD205 pKa = 2.96WTMQGWLVPVFICLTQRR222 pKa = 11.84LCLNTTEE229 pKa = 4.81SWMKK233 pKa = 9.96QLQNRR238 pKa = 11.84FAAVFLFARR247 pKa = 11.84FLNGKK252 pKa = 8.59EE253 pKa = 3.48IYY255 pKa = 7.86YY256 pKa = 10.23QKK258 pKa = 10.72VPGIMKK264 pKa = 10.28SGFLGTIMYY273 pKa = 10.7NSIAQVALHH282 pKa = 6.77IATKK286 pKa = 10.64LSLTGVVDD294 pKa = 3.95SDD296 pKa = 3.87GEE298 pKa = 4.25NYY300 pKa = 9.93ILPFAMGDD308 pKa = 3.53DD309 pKa = 4.69TIQLPEE315 pKa = 3.93NKK317 pKa = 9.5EE318 pKa = 3.94YY319 pKa = 11.19LEE321 pKa = 4.27KK322 pKa = 10.79LPKK325 pKa = 9.7FGCIIKK331 pKa = 10.1EE332 pKa = 4.07VAFSQVCEE340 pKa = 3.67FAGYY344 pKa = 10.88KK345 pKa = 8.35FTNKK349 pKa = 9.84YY350 pKa = 10.29CLPCYY355 pKa = 9.23EE356 pKa = 5.25DD357 pKa = 3.01KK358 pKa = 11.29HH359 pKa = 6.53KK360 pKa = 10.92FCLAHH365 pKa = 7.55LEE367 pKa = 4.04DD368 pKa = 5.25SYY370 pKa = 12.02TEE372 pKa = 4.04ALEE375 pKa = 4.37SYY377 pKa = 9.17MYY379 pKa = 10.46LYY381 pKa = 11.11SFDD384 pKa = 4.04PDD386 pKa = 3.09MYY388 pKa = 10.85QILRR392 pKa = 11.84EE393 pKa = 4.15KK394 pKa = 10.87LMEE397 pKa = 5.08ADD399 pKa = 3.56PSRR402 pKa = 11.84IKK404 pKa = 10.56SRR406 pKa = 11.84PYY408 pKa = 9.68LVRR411 pKa = 11.84WFNGYY416 pKa = 9.52

Molecular weight:
48.34 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KEA7|A0A1L3KEA7_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 23 OX=1922695 PE=4 SV=1
MM1 pKa = 7.71AEE3 pKa = 3.61VDD5 pKa = 3.94EE6 pKa = 5.21NILRR10 pKa = 11.84QRR12 pKa = 11.84WLMYY16 pKa = 9.84FFFYY20 pKa = 10.8SSGISIILHH29 pKa = 6.35IILFVYY35 pKa = 10.54LLYY38 pKa = 10.22LICMAAKK45 pKa = 9.99RR46 pKa = 11.84CICRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84DD53 pKa = 3.17RR54 pKa = 11.84STVEE58 pKa = 3.72TTLLRR63 pKa = 11.84EE64 pKa = 4.24GWSEE68 pKa = 4.38NRR70 pKa = 11.84ITTDD74 pKa = 2.51NSRR77 pKa = 11.84YY78 pKa = 8.69YY79 pKa = 10.7GAVPVSIPASPQRR92 pKa = 11.84TYY94 pKa = 10.55TVPLDD99 pKa = 4.52SSMSWDD105 pKa = 3.36AQAEE109 pKa = 4.14PGQYY113 pKa = 10.06VPEE116 pKa = 4.34TAWTHH121 pKa = 6.53DD122 pKa = 2.78KK123 pKa = 9.48VTRR126 pKa = 11.84VKK128 pKa = 9.71TDD130 pKa = 3.21NMPHH134 pKa = 6.4FSLRR138 pKa = 11.84VDD140 pKa = 3.39KK141 pKa = 10.89EE142 pKa = 3.91VLIEE146 pKa = 4.14GKK148 pKa = 8.19RR149 pKa = 11.84TRR151 pKa = 11.84CVAGNAVRR159 pKa = 11.84VEE161 pKa = 3.89NDD163 pKa = 2.79IVLPTHH169 pKa = 5.74MLEE172 pKa = 4.05DD173 pKa = 3.75AEE175 pKa = 5.55RR176 pKa = 11.84IFLMAPAKK184 pKa = 10.03RR185 pKa = 11.84VNGKK189 pKa = 9.24EE190 pKa = 4.15GEE192 pKa = 4.18WIQLEE197 pKa = 4.42IYY199 pKa = 10.58SDD201 pKa = 3.56EE202 pKa = 4.34TEE204 pKa = 4.27QLDD207 pKa = 3.49SDD209 pKa = 4.25LSVVRR214 pKa = 11.84SSRR217 pKa = 11.84FYY219 pKa = 9.87ITDD222 pKa = 3.21VFEE225 pKa = 4.76KK226 pKa = 10.74LGLTAPILGTVNSMVFVTVTSFCLVKK252 pKa = 10.36IEE254 pKa = 3.73GMQYY258 pKa = 10.46KK259 pKa = 9.76EE260 pKa = 3.3VDD262 pKa = 2.75GFYY265 pKa = 10.39RR266 pKa = 11.84GRR268 pKa = 11.84GDD270 pKa = 4.22LSPSKK275 pKa = 10.49RR276 pKa = 11.84KK277 pKa = 9.89LGYY280 pKa = 9.08TDD282 pKa = 5.58FNGSTYY288 pKa = 8.85PTFSGSPYY296 pKa = 9.93HH297 pKa = 7.7VGEE300 pKa = 4.2VLYY303 pKa = 10.34GIHH306 pKa = 6.93LSGSTRR312 pKa = 11.84VNMGLDD318 pKa = 3.31VQYY321 pKa = 11.22IRR323 pKa = 11.84ILLMKK328 pKa = 10.08VDD330 pKa = 4.19GQIQIPEE337 pKa = 4.24SSSEE341 pKa = 3.94WLEE344 pKa = 4.15KK345 pKa = 10.56LSKK348 pKa = 9.17QQKK351 pKa = 6.56QRR353 pKa = 11.84KK354 pKa = 5.52TRR356 pKa = 11.84LVVSRR361 pKa = 11.84HH362 pKa = 5.74ASDD365 pKa = 3.82PDD367 pKa = 3.69YY368 pKa = 11.44VIVRR372 pKa = 11.84QGGGYY377 pKa = 9.7KK378 pKa = 9.36VAHH381 pKa = 6.13YY382 pKa = 11.03KK383 pKa = 10.72DD384 pKa = 3.84FEE386 pKa = 4.41KK387 pKa = 10.72FFPSGTYY394 pKa = 10.26DD395 pKa = 2.96VGTDD399 pKa = 3.27TAYY402 pKa = 10.48MMPEE406 pKa = 4.14SPDD409 pKa = 3.24EE410 pKa = 4.02PVPTKK415 pKa = 10.67GKK417 pKa = 10.71SIDD420 pKa = 3.61DD421 pKa = 4.29LFVKK425 pKa = 9.63TCQDD429 pKa = 2.63ISLEE433 pKa = 4.07AKK435 pKa = 8.5TLKK438 pKa = 10.79LKK440 pKa = 10.32MPEE443 pKa = 4.17KK444 pKa = 10.37KK445 pKa = 10.17EE446 pKa = 3.58KK447 pKa = 9.32TAQEE451 pKa = 3.95KK452 pKa = 10.65AKK454 pKa = 10.79EE455 pKa = 3.64IDD457 pKa = 3.4QEE459 pKa = 4.43FKK461 pKa = 10.76KK462 pKa = 11.0ACDD465 pKa = 3.63TLDD468 pKa = 3.48KK469 pKa = 11.17YY470 pKa = 11.28SNKK473 pKa = 10.24LLDD476 pKa = 4.54KK477 pKa = 11.09EE478 pKa = 4.34NTDD481 pKa = 3.23KK482 pKa = 11.43QKK484 pKa = 10.76VVQSYY489 pKa = 7.12EE490 pKa = 3.93QEE492 pKa = 4.15VEE494 pKa = 4.05DD495 pKa = 4.2NYY497 pKa = 11.51SVVSEE502 pKa = 4.22MTSLSHH508 pKa = 7.28DD509 pKa = 3.83VPSKK513 pKa = 10.17EE514 pKa = 3.94VEE516 pKa = 3.84AGLEE520 pKa = 4.19HH521 pKa = 7.2KK522 pKa = 10.48LKK524 pKa = 11.04LMSDD528 pKa = 3.66NLAAMAEE535 pKa = 4.12ILKK538 pKa = 10.52QSAFVVEE545 pKa = 4.78DD546 pKa = 3.72KK547 pKa = 10.76VAKK550 pKa = 9.72PVSQVIYY557 pKa = 9.82RR558 pKa = 11.84HH559 pKa = 5.54NKK561 pKa = 9.1EE562 pKa = 3.22IDD564 pKa = 3.63EE565 pKa = 4.54IRR567 pKa = 11.84DD568 pKa = 3.31EE569 pKa = 4.87LIDD572 pKa = 3.73YY573 pKa = 10.81KK574 pKa = 11.15SITGRR579 pKa = 11.84AFTQLNQKK587 pKa = 9.42IIKK590 pKa = 9.32RR591 pKa = 11.84LPPEE595 pKa = 4.12EE596 pKa = 4.09VSVPEE601 pKa = 4.17SPMVPVEE608 pKa = 3.75VSYY611 pKa = 11.66EE612 pKa = 3.89EE613 pKa = 4.56DD614 pKa = 3.3QVHH617 pKa = 6.42SFDD620 pKa = 3.97PKK622 pKa = 10.7EE623 pKa = 4.04EE624 pKa = 4.37GFSEE628 pKa = 4.26PSEE631 pKa = 4.25NRR633 pKa = 11.84DD634 pKa = 3.35QEE636 pKa = 4.3LVEE639 pKa = 3.95MHH641 pKa = 7.09KK642 pKa = 10.37EE643 pKa = 3.65IQSFKK648 pKa = 10.82SQLGQLKK655 pKa = 10.11NLNQTLIAEE664 pKa = 4.15NLVLKK669 pKa = 8.14EE670 pKa = 4.08QKK672 pKa = 10.12EE673 pKa = 4.11KK674 pKa = 11.03ALEE677 pKa = 3.78NWRR680 pKa = 11.84LEE682 pKa = 4.17EE683 pKa = 4.86QKK685 pKa = 10.99LNQQKK690 pKa = 10.64EE691 pKa = 4.32IVNSHH696 pKa = 6.66RR697 pKa = 11.84EE698 pKa = 3.63LHH700 pKa = 6.14HH701 pKa = 5.96QVKK704 pKa = 9.12QQASDD709 pKa = 3.95LGHH712 pKa = 6.06QMNQVMEE719 pKa = 4.45QISSIDD725 pKa = 3.44KK726 pKa = 9.53MKK728 pKa = 11.08KK729 pKa = 9.18EE730 pKa = 4.05IQKK733 pKa = 10.75KK734 pKa = 8.64MEE736 pKa = 4.74DD737 pKa = 3.55LNKK740 pKa = 10.68EE741 pKa = 4.13MVNQKK746 pKa = 7.49QTQQSKK752 pKa = 10.71KK753 pKa = 10.33LYY755 pKa = 9.31NSQPKK760 pKa = 7.06MTPEE764 pKa = 4.08LEE766 pKa = 4.05IKK768 pKa = 10.41KK769 pKa = 10.21LLEE772 pKa = 4.03EE773 pKa = 5.14EE774 pKa = 4.36GLKK777 pKa = 8.21WTFFQVLYY785 pKa = 11.06NSALHH790 pKa = 6.18TNNISPYY797 pKa = 8.03VNGYY801 pKa = 10.11VMSQAKK807 pKa = 9.0KK808 pKa = 9.55VKK810 pKa = 9.74VNKK813 pKa = 9.97EE814 pKa = 3.44KK815 pKa = 10.86LLNPKK820 pKa = 9.32QVAHH824 pKa = 5.75GTIDD828 pKa = 3.69LQQLKK833 pKa = 9.06TNWLTFIEE841 pKa = 4.41TLKK844 pKa = 10.3MDD846 pKa = 3.78GSKK849 pKa = 10.57NLKK852 pKa = 9.45EE853 pKa = 4.03LEE855 pKa = 4.36KK856 pKa = 10.31IYY858 pKa = 11.1ALVVEE863 pKa = 4.88

Molecular weight:
99.39 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1279

416

863

639.5

73.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.378 ± 0.342

1.329 ± 0.548

5.629 ± 0.071

8.444 ± 0.996

3.753 ± 0.783

4.691 ± 0.427

2.111 ± 0.096

5.395 ± 0.068

8.991 ± 0.54

8.991 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.44 ± 0.33

4.378 ± 0.342

4.066 ± 0.133

4.926 ± 1.041

3.909 ± 0.277

7.428 ± 0.11

4.613 ± 0.636

7.349 ± 0.806

1.486 ± 0.468

4.691 ± 0.918

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski