Beihai sobemo-like virus 23
Average proteome isoelectric point is 5.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KEA7|A0A1L3KEA7_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 23 OX=1922695 PE=4 SV=1
MM1 pKa = 8.06 DD2 pKa = 5.85 DD3 pKa = 3.27 AAKK6 pKa = 10.18 RR7 pKa = 11.84 QSLCYY12 pKa = 9.58 HH13 pKa = 6.38 GRR15 pKa = 11.84 RR16 pKa = 11.84 NYY18 pKa = 8.8 QVSMNSIHH26 pKa = 7.06 PNDD29 pKa = 3.63 SLEE32 pKa = 4.03 IVDD35 pKa = 4.48 ILEE38 pKa = 4.04 EE39 pKa = 4.7 AYY41 pKa = 8.85 TKK43 pKa = 10.77 CRR45 pKa = 11.84 FKK47 pKa = 11.1 VPKK50 pKa = 10.43 FNFSWFYY57 pKa = 11.48 NEE59 pKa = 3.87 VLHH62 pKa = 5.77 TVEE65 pKa = 5.4 FSSSPGYY72 pKa = 9.82 PMNEE76 pKa = 3.99 KK77 pKa = 10.69 YY78 pKa = 9.17 STNAEE83 pKa = 3.95 LFGWDD88 pKa = 4.36 GEE90 pKa = 4.27 NLDD93 pKa = 3.38 VDD95 pKa = 4.11 KK96 pKa = 11.37 VYY98 pKa = 10.91 EE99 pKa = 4.14 VYY101 pKa = 10.79 KK102 pKa = 10.49 KK103 pKa = 10.77 VKK105 pKa = 9.59 QRR107 pKa = 11.84 LINLLHH113 pKa = 6.56 GPEE116 pKa = 4.67 ADD118 pKa = 4.81 PINLFIKK125 pKa = 10.4 DD126 pKa = 3.89 EE127 pKa = 3.99 PHH129 pKa = 6.46 KK130 pKa = 10.61 KK131 pKa = 10.15 SKK133 pKa = 10.98 EE134 pKa = 3.87 EE135 pKa = 3.6 VGAWRR140 pKa = 11.84 LISGASIIDD149 pKa = 3.68 VMVDD153 pKa = 3.34 RR154 pKa = 11.84 YY155 pKa = 11.19 LFGTLSDD162 pKa = 3.51 MMINKK167 pKa = 10.04 CYY169 pKa = 10.31 DD170 pKa = 3.27 IPNKK174 pKa = 10.53 AGWSPVSGGYY184 pKa = 9.92 KK185 pKa = 9.34 WMYY188 pKa = 10.33 SVLGNSLKK196 pKa = 10.46 MMADD200 pKa = 3.1 KK201 pKa = 11.13 SSWDD205 pKa = 2.96 WTMQGWLVPVFICLTQRR222 pKa = 11.84 LCLNTTEE229 pKa = 4.81 SWMKK233 pKa = 9.96 QLQNRR238 pKa = 11.84 FAAVFLFARR247 pKa = 11.84 FLNGKK252 pKa = 8.59 EE253 pKa = 3.48 IYY255 pKa = 7.86 YY256 pKa = 10.23 QKK258 pKa = 10.72 VPGIMKK264 pKa = 10.28 SGFLGTIMYY273 pKa = 10.7 NSIAQVALHH282 pKa = 6.77 IATKK286 pKa = 10.64 LSLTGVVDD294 pKa = 3.95 SDD296 pKa = 3.87 GEE298 pKa = 4.25 NYY300 pKa = 9.93 ILPFAMGDD308 pKa = 3.53 DD309 pKa = 4.69 TIQLPEE315 pKa = 3.93 NKK317 pKa = 9.5 EE318 pKa = 3.94 YY319 pKa = 11.19 LEE321 pKa = 4.27 KK322 pKa = 10.79 LPKK325 pKa = 9.7 FGCIIKK331 pKa = 10.1 EE332 pKa = 4.07 VAFSQVCEE340 pKa = 3.67 FAGYY344 pKa = 10.88 KK345 pKa = 8.35 FTNKK349 pKa = 9.84 YY350 pKa = 10.29 CLPCYY355 pKa = 9.23 EE356 pKa = 5.25 DD357 pKa = 3.01 KK358 pKa = 11.29 HH359 pKa = 6.53 KK360 pKa = 10.92 FCLAHH365 pKa = 7.55 LEE367 pKa = 4.04 DD368 pKa = 5.25 SYY370 pKa = 12.02 TEE372 pKa = 4.04 ALEE375 pKa = 4.37 SYY377 pKa = 9.17 MYY379 pKa = 10.46 LYY381 pKa = 11.11 SFDD384 pKa = 4.04 PDD386 pKa = 3.09 MYY388 pKa = 10.85 QILRR392 pKa = 11.84 EE393 pKa = 4.15 KK394 pKa = 10.87 LMEE397 pKa = 5.08 ADD399 pKa = 3.56 PSRR402 pKa = 11.84 IKK404 pKa = 10.56 SRR406 pKa = 11.84 PYY408 pKa = 9.68 LVRR411 pKa = 11.84 WFNGYY416 pKa = 9.52
Molecular weight: 48.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.653
IPC2_protein 5.817
IPC_protein 5.855
Toseland 6.109
ProMoST 6.198
Dawson 6.109
Bjellqvist 6.122
Wikipedia 6.109
Rodwell 6.084
Grimsley 6.198
Solomon 6.109
Lehninger 6.097
Nozaki 6.364
DTASelect 6.547
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.465
Patrickios 3.63
IPC_peptide 6.122
IPC2_peptide 6.389
IPC2.peptide.svr19 6.456
Protein with the highest isoelectric point:
>tr|A0A1L3KEA7|A0A1L3KEA7_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 23 OX=1922695 PE=4 SV=1
MM1 pKa = 7.71 AEE3 pKa = 3.61 VDD5 pKa = 3.94 EE6 pKa = 5.21 NILRR10 pKa = 11.84 QRR12 pKa = 11.84 WLMYY16 pKa = 9.84 FFFYY20 pKa = 10.8 SSGISIILHH29 pKa = 6.35 IILFVYY35 pKa = 10.54 LLYY38 pKa = 10.22 LICMAAKK45 pKa = 9.99 RR46 pKa = 11.84 CICRR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 DD53 pKa = 3.17 RR54 pKa = 11.84 STVEE58 pKa = 3.72 TTLLRR63 pKa = 11.84 EE64 pKa = 4.24 GWSEE68 pKa = 4.38 NRR70 pKa = 11.84 ITTDD74 pKa = 2.51 NSRR77 pKa = 11.84 YY78 pKa = 8.69 YY79 pKa = 10.7 GAVPVSIPASPQRR92 pKa = 11.84 TYY94 pKa = 10.55 TVPLDD99 pKa = 4.52 SSMSWDD105 pKa = 3.36 AQAEE109 pKa = 4.14 PGQYY113 pKa = 10.06 VPEE116 pKa = 4.34 TAWTHH121 pKa = 6.53 DD122 pKa = 2.78 KK123 pKa = 9.48 VTRR126 pKa = 11.84 VKK128 pKa = 9.71 TDD130 pKa = 3.21 NMPHH134 pKa = 6.4 FSLRR138 pKa = 11.84 VDD140 pKa = 3.39 KK141 pKa = 10.89 EE142 pKa = 3.91 VLIEE146 pKa = 4.14 GKK148 pKa = 8.19 RR149 pKa = 11.84 TRR151 pKa = 11.84 CVAGNAVRR159 pKa = 11.84 VEE161 pKa = 3.89 NDD163 pKa = 2.79 IVLPTHH169 pKa = 5.74 MLEE172 pKa = 4.05 DD173 pKa = 3.75 AEE175 pKa = 5.55 RR176 pKa = 11.84 IFLMAPAKK184 pKa = 10.03 RR185 pKa = 11.84 VNGKK189 pKa = 9.24 EE190 pKa = 4.15 GEE192 pKa = 4.18 WIQLEE197 pKa = 4.42 IYY199 pKa = 10.58 SDD201 pKa = 3.56 EE202 pKa = 4.34 TEE204 pKa = 4.27 QLDD207 pKa = 3.49 SDD209 pKa = 4.25 LSVVRR214 pKa = 11.84 SSRR217 pKa = 11.84 FYY219 pKa = 9.87 ITDD222 pKa = 3.21 VFEE225 pKa = 4.76 KK226 pKa = 10.74 LGLTAPILGTVNSMVFVTVTSFCLVKK252 pKa = 10.36 IEE254 pKa = 3.73 GMQYY258 pKa = 10.46 KK259 pKa = 9.76 EE260 pKa = 3.3 VDD262 pKa = 2.75 GFYY265 pKa = 10.39 RR266 pKa = 11.84 GRR268 pKa = 11.84 GDD270 pKa = 4.22 LSPSKK275 pKa = 10.49 RR276 pKa = 11.84 KK277 pKa = 9.89 LGYY280 pKa = 9.08 TDD282 pKa = 5.58 FNGSTYY288 pKa = 8.85 PTFSGSPYY296 pKa = 9.93 HH297 pKa = 7.7 VGEE300 pKa = 4.2 VLYY303 pKa = 10.34 GIHH306 pKa = 6.93 LSGSTRR312 pKa = 11.84 VNMGLDD318 pKa = 3.31 VQYY321 pKa = 11.22 IRR323 pKa = 11.84 ILLMKK328 pKa = 10.08 VDD330 pKa = 4.19 GQIQIPEE337 pKa = 4.24 SSSEE341 pKa = 3.94 WLEE344 pKa = 4.15 KK345 pKa = 10.56 LSKK348 pKa = 9.17 QQKK351 pKa = 6.56 QRR353 pKa = 11.84 KK354 pKa = 5.52 TRR356 pKa = 11.84 LVVSRR361 pKa = 11.84 HH362 pKa = 5.74 ASDD365 pKa = 3.82 PDD367 pKa = 3.69 YY368 pKa = 11.44 VIVRR372 pKa = 11.84 QGGGYY377 pKa = 9.7 KK378 pKa = 9.36 VAHH381 pKa = 6.13 YY382 pKa = 11.03 KK383 pKa = 10.72 DD384 pKa = 3.84 FEE386 pKa = 4.41 KK387 pKa = 10.72 FFPSGTYY394 pKa = 10.26 DD395 pKa = 2.96 VGTDD399 pKa = 3.27 TAYY402 pKa = 10.48 MMPEE406 pKa = 4.14 SPDD409 pKa = 3.24 EE410 pKa = 4.02 PVPTKK415 pKa = 10.67 GKK417 pKa = 10.71 SIDD420 pKa = 3.61 DD421 pKa = 4.29 LFVKK425 pKa = 9.63 TCQDD429 pKa = 2.63 ISLEE433 pKa = 4.07 AKK435 pKa = 8.5 TLKK438 pKa = 10.79 LKK440 pKa = 10.32 MPEE443 pKa = 4.17 KK444 pKa = 10.37 KK445 pKa = 10.17 EE446 pKa = 3.58 KK447 pKa = 9.32 TAQEE451 pKa = 3.95 KK452 pKa = 10.65 AKK454 pKa = 10.79 EE455 pKa = 3.64 IDD457 pKa = 3.4 QEE459 pKa = 4.43 FKK461 pKa = 10.76 KK462 pKa = 11.0 ACDD465 pKa = 3.63 TLDD468 pKa = 3.48 KK469 pKa = 11.17 YY470 pKa = 11.28 SNKK473 pKa = 10.24 LLDD476 pKa = 4.54 KK477 pKa = 11.09 EE478 pKa = 4.34 NTDD481 pKa = 3.23 KK482 pKa = 11.43 QKK484 pKa = 10.76 VVQSYY489 pKa = 7.12 EE490 pKa = 3.93 QEE492 pKa = 4.15 VEE494 pKa = 4.05 DD495 pKa = 4.2 NYY497 pKa = 11.51 SVVSEE502 pKa = 4.22 MTSLSHH508 pKa = 7.28 DD509 pKa = 3.83 VPSKK513 pKa = 10.17 EE514 pKa = 3.94 VEE516 pKa = 3.84 AGLEE520 pKa = 4.19 HH521 pKa = 7.2 KK522 pKa = 10.48 LKK524 pKa = 11.04 LMSDD528 pKa = 3.66 NLAAMAEE535 pKa = 4.12 ILKK538 pKa = 10.52 QSAFVVEE545 pKa = 4.78 DD546 pKa = 3.72 KK547 pKa = 10.76 VAKK550 pKa = 9.72 PVSQVIYY557 pKa = 9.82 RR558 pKa = 11.84 HH559 pKa = 5.54 NKK561 pKa = 9.1 EE562 pKa = 3.22 IDD564 pKa = 3.63 EE565 pKa = 4.54 IRR567 pKa = 11.84 DD568 pKa = 3.31 EE569 pKa = 4.87 LIDD572 pKa = 3.73 YY573 pKa = 10.81 KK574 pKa = 11.15 SITGRR579 pKa = 11.84 AFTQLNQKK587 pKa = 9.42 IIKK590 pKa = 9.32 RR591 pKa = 11.84 LPPEE595 pKa = 4.12 EE596 pKa = 4.09 VSVPEE601 pKa = 4.17 SPMVPVEE608 pKa = 3.75 VSYY611 pKa = 11.66 EE612 pKa = 3.89 EE613 pKa = 4.56 DD614 pKa = 3.3 QVHH617 pKa = 6.42 SFDD620 pKa = 3.97 PKK622 pKa = 10.7 EE623 pKa = 4.04 EE624 pKa = 4.37 GFSEE628 pKa = 4.26 PSEE631 pKa = 4.25 NRR633 pKa = 11.84 DD634 pKa = 3.35 QEE636 pKa = 4.3 LVEE639 pKa = 3.95 MHH641 pKa = 7.09 KK642 pKa = 10.37 EE643 pKa = 3.65 IQSFKK648 pKa = 10.82 SQLGQLKK655 pKa = 10.11 NLNQTLIAEE664 pKa = 4.15 NLVLKK669 pKa = 8.14 EE670 pKa = 4.08 QKK672 pKa = 10.12 EE673 pKa = 4.11 KK674 pKa = 11.03 ALEE677 pKa = 3.78 NWRR680 pKa = 11.84 LEE682 pKa = 4.17 EE683 pKa = 4.86 QKK685 pKa = 10.99 LNQQKK690 pKa = 10.64 EE691 pKa = 4.32 IVNSHH696 pKa = 6.66 RR697 pKa = 11.84 EE698 pKa = 3.63 LHH700 pKa = 6.14 HH701 pKa = 5.96 QVKK704 pKa = 9.12 QQASDD709 pKa = 3.95 LGHH712 pKa = 6.06 QMNQVMEE719 pKa = 4.45 QISSIDD725 pKa = 3.44 KK726 pKa = 9.53 MKK728 pKa = 11.08 KK729 pKa = 9.18 EE730 pKa = 4.05 IQKK733 pKa = 10.75 KK734 pKa = 8.64 MEE736 pKa = 4.74 DD737 pKa = 3.55 LNKK740 pKa = 10.68 EE741 pKa = 4.13 MVNQKK746 pKa = 7.49 QTQQSKK752 pKa = 10.71 KK753 pKa = 10.33 LYY755 pKa = 9.31 NSQPKK760 pKa = 7.06 MTPEE764 pKa = 4.08 LEE766 pKa = 4.05 IKK768 pKa = 10.41 KK769 pKa = 10.21 LLEE772 pKa = 4.03 EE773 pKa = 5.14 EE774 pKa = 4.36 GLKK777 pKa = 8.21 WTFFQVLYY785 pKa = 11.06 NSALHH790 pKa = 6.18 TNNISPYY797 pKa = 8.03 VNGYY801 pKa = 10.11 VMSQAKK807 pKa = 9.0 KK808 pKa = 9.55 VKK810 pKa = 9.74 VNKK813 pKa = 9.97 EE814 pKa = 3.44 KK815 pKa = 10.86 LLNPKK820 pKa = 9.32 QVAHH824 pKa = 5.75 GTIDD828 pKa = 3.69 LQQLKK833 pKa = 9.06 TNWLTFIEE841 pKa = 4.41 TLKK844 pKa = 10.3 MDD846 pKa = 3.78 GSKK849 pKa = 10.57 NLKK852 pKa = 9.45 EE853 pKa = 4.03 LEE855 pKa = 4.36 KK856 pKa = 10.31 IYY858 pKa = 11.1 ALVVEE863 pKa = 4.88
Molecular weight: 99.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.804
IPC2_protein 5.779
IPC_protein 5.817
Toseland 6.186
ProMoST 6.071
Dawson 6.033
Bjellqvist 6.059
Wikipedia 6.02
Rodwell 6.008
Grimsley 6.338
Solomon 6.033
Lehninger 6.02
Nozaki 6.275
DTASelect 6.453
Thurlkill 6.453
EMBOSS 6.402
Sillero 6.376
Patrickios 4.393
IPC_peptide 6.046
IPC2_peptide 6.326
IPC2.peptide.svr19 6.416
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1279
416
863
639.5
73.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.378 ± 0.342
1.329 ± 0.548
5.629 ± 0.071
8.444 ± 0.996
3.753 ± 0.783
4.691 ± 0.427
2.111 ± 0.096
5.395 ± 0.068
8.991 ± 0.54
8.991 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.44 ± 0.33
4.378 ± 0.342
4.066 ± 0.133
4.926 ± 1.041
3.909 ± 0.277
7.428 ± 0.11
4.613 ± 0.636
7.349 ± 0.806
1.486 ± 0.468
4.691 ± 0.918
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here