Spinach amalgavirus 1
Average proteome isoelectric point is 7.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6S3T3|A0A1W6S3T3_9VIRU Putative coat protein OS=Spinach amalgavirus 1 OX=1985164 PE=4 SV=1
MM1 pKa = 7.7 AGLEE5 pKa = 4.4 GEE7 pKa = 4.43 GSEE10 pKa = 5.28 GIHH13 pKa = 6.25 LVEE16 pKa = 4.4 KK17 pKa = 10.0 TPKK20 pKa = 9.84 QEE22 pKa = 3.94 QEE24 pKa = 4.13 EE25 pKa = 4.9 LFTASEE31 pKa = 4.42 KK32 pKa = 10.67 LRR34 pKa = 11.84 EE35 pKa = 4.09 AGIPLGAFDD44 pKa = 4.75 RR45 pKa = 11.84 NAIIKK50 pKa = 10.1 AGHH53 pKa = 5.96 SFNGYY58 pKa = 8.1 MKK60 pKa = 9.83 MIKK63 pKa = 10.25 YY64 pKa = 6.31 VTNYY68 pKa = 9.68 TEE70 pKa = 5.41 GGFFDD75 pKa = 4.06 TLLVMGGSKK84 pKa = 10.31 KK85 pKa = 10.51 LYY87 pKa = 8.9 PIHH90 pKa = 7.34 SKK92 pKa = 9.41 MDD94 pKa = 3.05 HH95 pKa = 6.13 FGFVRR100 pKa = 11.84 FARR103 pKa = 11.84 WLQSKK108 pKa = 9.76 EE109 pKa = 4.15 GQDD112 pKa = 3.97 EE113 pKa = 4.86 IYY115 pKa = 10.34 TLQRR119 pKa = 11.84 EE120 pKa = 4.39 QKK122 pKa = 9.29 LLRR125 pKa = 11.84 KK126 pKa = 9.64 AGEE129 pKa = 4.43 SLTPTQMIKK138 pKa = 10.71 NDD140 pKa = 3.54 VFNLIRR146 pKa = 11.84 TEE148 pKa = 4.18 FSRR151 pKa = 11.84 AMKK154 pKa = 10.53 AEE156 pKa = 3.91 RR157 pKa = 11.84 EE158 pKa = 4.1 KK159 pKa = 11.18 FEE161 pKa = 4.04 QEE163 pKa = 3.3 KK164 pKa = 11.34 DD165 pKa = 3.09 EE166 pKa = 4.13 LRR168 pKa = 11.84 RR169 pKa = 11.84 LLRR172 pKa = 11.84 QKK174 pKa = 10.43 EE175 pKa = 4.12 RR176 pKa = 11.84 EE177 pKa = 3.67 EE178 pKa = 3.77 RR179 pKa = 11.84 QAFKK183 pKa = 11.09 NLQEE187 pKa = 4.12 KK188 pKa = 8.96 FAPISFYY195 pKa = 10.88 RR196 pKa = 11.84 EE197 pKa = 3.81 PTDD200 pKa = 3.47 EE201 pKa = 4.17 EE202 pKa = 4.37 VGIAAYY208 pKa = 10.04 EE209 pKa = 4.09 MYY211 pKa = 10.67 EE212 pKa = 3.86 NEE214 pKa = 3.6 ARR216 pKa = 11.84 KK217 pKa = 9.86 NGKK220 pKa = 7.46 TPMSRR225 pKa = 11.84 YY226 pKa = 9.72 HH227 pKa = 6.97 GGDD230 pKa = 2.89 VYY232 pKa = 11.34 ARR234 pKa = 11.84 QHH236 pKa = 6.27 FSQAARR242 pKa = 11.84 EE243 pKa = 3.93 LAQVYY248 pKa = 8.03 FASNPEE254 pKa = 3.88 NQDD257 pKa = 2.56 IMEE260 pKa = 4.59 RR261 pKa = 11.84 FMKK264 pKa = 10.29 EE265 pKa = 2.97 RR266 pKa = 11.84 FLFFRR271 pKa = 11.84 KK272 pKa = 7.71 TADD275 pKa = 3.14 EE276 pKa = 4.3 SGEE279 pKa = 3.98 RR280 pKa = 11.84 TARR283 pKa = 11.84 MQLVGIGGEE292 pKa = 4.18 KK293 pKa = 10.21 AVEE296 pKa = 3.87 DD297 pKa = 4.19 ALAEE301 pKa = 4.0 EE302 pKa = 4.51 AKK304 pKa = 10.62 AYY306 pKa = 10.6 GDD308 pKa = 4.43 DD309 pKa = 4.12 PDD311 pKa = 5.2 RR312 pKa = 11.84 DD313 pKa = 3.93 SAGAGAAGEE322 pKa = 4.21 VQGASKK328 pKa = 10.22 KK329 pKa = 10.01 AKK331 pKa = 9.19 PRR333 pKa = 11.84 FVSKK337 pKa = 10.55 PPTGRR342 pKa = 11.84 GKK344 pKa = 10.27 AAAGDD349 pKa = 3.97 QPEE352 pKa = 4.82 GGADD356 pKa = 3.44 QPGSEE361 pKa = 4.45 DD362 pKa = 5.7 AEE364 pKa = 4.44 DD365 pKa = 3.87 QSDD368 pKa = 3.81 TSSTLSRR375 pKa = 11.84 PRR377 pKa = 11.84 KK378 pKa = 6.11 TARR381 pKa = 11.84 NTRR384 pKa = 11.84 SKK386 pKa = 10.8 KK387 pKa = 10.2
Molecular weight: 43.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.66
IPC2_protein 6.634
IPC_protein 7.044
Toseland 7.673
ProMoST 7.541
Dawson 7.483
Bjellqvist 7.059
Wikipedia 7.512
Rodwell 7.439
Grimsley 7.629
Solomon 7.746
Lehninger 7.717
Nozaki 7.366
DTASelect 7.614
Thurlkill 7.658
EMBOSS 7.863
Sillero 7.658
Patrickios 4.851
IPC_peptide 7.746
IPC2_peptide 6.883
IPC2.peptide.svr19 7.025
Protein with the highest isoelectric point:
>tr|A0A1W6S3T3|A0A1W6S3T3_9VIRU Putative coat protein OS=Spinach amalgavirus 1 OX=1985164 PE=4 SV=1
MM1 pKa = 7.7 AGLEE5 pKa = 4.4 GEE7 pKa = 4.43 GSEE10 pKa = 5.28 GIHH13 pKa = 6.25 LVEE16 pKa = 4.4 KK17 pKa = 10.0 TPKK20 pKa = 9.84 QEE22 pKa = 3.94 QEE24 pKa = 4.13 EE25 pKa = 4.9 LFTASEE31 pKa = 4.42 KK32 pKa = 10.67 LRR34 pKa = 11.84 EE35 pKa = 4.09 AGIPLGAFDD44 pKa = 4.75 RR45 pKa = 11.84 NAIIKK50 pKa = 10.1 AGHH53 pKa = 5.96 SFNGYY58 pKa = 8.1 MKK60 pKa = 9.83 MIKK63 pKa = 10.25 YY64 pKa = 6.31 VTNYY68 pKa = 9.68 TEE70 pKa = 5.41 GGFFDD75 pKa = 4.06 TLLVMGGSKK84 pKa = 10.31 KK85 pKa = 10.51 LYY87 pKa = 8.9 PIHH90 pKa = 7.34 SKK92 pKa = 9.41 MDD94 pKa = 3.05 HH95 pKa = 6.13 FGFVRR100 pKa = 11.84 FARR103 pKa = 11.84 WLQSKK108 pKa = 9.76 EE109 pKa = 4.15 GQDD112 pKa = 3.97 EE113 pKa = 4.86 IYY115 pKa = 10.34 TLQRR119 pKa = 11.84 EE120 pKa = 4.39 QKK122 pKa = 9.29 LLRR125 pKa = 11.84 KK126 pKa = 9.64 AGEE129 pKa = 4.43 SLTPTQMIKK138 pKa = 10.71 NDD140 pKa = 3.54 VFNLIRR146 pKa = 11.84 TEE148 pKa = 4.18 FSRR151 pKa = 11.84 AMKK154 pKa = 10.53 AEE156 pKa = 3.91 RR157 pKa = 11.84 EE158 pKa = 4.1 KK159 pKa = 11.18 FEE161 pKa = 4.04 QEE163 pKa = 3.3 KK164 pKa = 11.34 DD165 pKa = 3.09 EE166 pKa = 4.13 LRR168 pKa = 11.84 RR169 pKa = 11.84 LLRR172 pKa = 11.84 QKK174 pKa = 10.43 EE175 pKa = 4.12 RR176 pKa = 11.84 EE177 pKa = 3.67 EE178 pKa = 3.77 RR179 pKa = 11.84 QAFKK183 pKa = 11.09 NLQEE187 pKa = 4.12 KK188 pKa = 8.96 FAPISFYY195 pKa = 10.88 RR196 pKa = 11.84 EE197 pKa = 3.81 PTDD200 pKa = 3.47 EE201 pKa = 4.17 EE202 pKa = 4.37 VGIAAYY208 pKa = 10.04 EE209 pKa = 4.09 MYY211 pKa = 10.67 EE212 pKa = 3.86 NEE214 pKa = 3.6 ARR216 pKa = 11.84 KK217 pKa = 9.86 NGKK220 pKa = 7.46 TPMSRR225 pKa = 11.84 YY226 pKa = 9.72 HH227 pKa = 6.97 GGDD230 pKa = 2.89 VYY232 pKa = 11.34 ARR234 pKa = 11.84 QHH236 pKa = 6.27 FSQAARR242 pKa = 11.84 EE243 pKa = 3.93 LAQVYY248 pKa = 8.03 FASNPEE254 pKa = 3.88 NQDD257 pKa = 2.56 IMEE260 pKa = 4.59 RR261 pKa = 11.84 FMKK264 pKa = 10.3 EE265 pKa = 3.01 RR266 pKa = 11.84 FLFFGRR272 pKa = 11.84 PPMNLGSAQQEE283 pKa = 4.61 CSWLALVEE291 pKa = 4.16 RR292 pKa = 11.84 KK293 pKa = 8.72 LWKK296 pKa = 9.69 MPLQRR301 pKa = 11.84 RR302 pKa = 11.84 LRR304 pKa = 11.84 LTGMIPIGILQAPGRR319 pKa = 11.84 RR320 pKa = 11.84 GRR322 pKa = 11.84 CRR324 pKa = 11.84 EE325 pKa = 3.98 LRR327 pKa = 11.84 RR328 pKa = 11.84 KK329 pKa = 10.17 LSPDD333 pKa = 3.26 LLASPRR339 pKa = 11.84 QVGVKK344 pKa = 9.43 LQQGISRR351 pKa = 11.84 KK352 pKa = 9.74 VEE354 pKa = 3.92 LTSLGPKK361 pKa = 8.44 MLRR364 pKa = 11.84 TSRR367 pKa = 11.84 IRR369 pKa = 11.84 VLRR372 pKa = 11.84 SLGPEE377 pKa = 3.84 RR378 pKa = 11.84 PRR380 pKa = 11.84 AIPVARR386 pKa = 11.84 SRR388 pKa = 11.84 FEE390 pKa = 3.96 AGVRR394 pKa = 11.84 KK395 pKa = 10.21 VIGGGAMRR403 pKa = 11.84 SWEE406 pKa = 4.43 VDD408 pKa = 2.97 SQMYY412 pKa = 10.23 RR413 pKa = 11.84 GGGNSADD420 pKa = 3.82 ALRR423 pKa = 11.84 LLGQARR429 pKa = 11.84 DD430 pKa = 3.89 DD431 pKa = 3.73 RR432 pKa = 11.84 PGAFLSGKK440 pKa = 8.03 FTQASARR447 pKa = 11.84 LALLLPNNLDD457 pKa = 3.5 VPDD460 pKa = 4.4 GAKK463 pKa = 8.35 STRR466 pKa = 11.84 MKK468 pKa = 10.89 NFNNDD473 pKa = 2.39 ATAGPFLRR481 pKa = 11.84 SFGIKK486 pKa = 9.75 GKK488 pKa = 10.75 YY489 pKa = 7.62 GLKK492 pKa = 10.15 RR493 pKa = 11.84 KK494 pKa = 10.1 LEE496 pKa = 4.14 EE497 pKa = 4.18 EE498 pKa = 3.56 MWRR501 pKa = 11.84 YY502 pKa = 9.62 YY503 pKa = 11.08 DD504 pKa = 4.59 DD505 pKa = 4.21 YY506 pKa = 11.34 GAGRR510 pKa = 11.84 IDD512 pKa = 3.73 SSGLPFFTARR522 pKa = 11.84 VGFRR526 pKa = 11.84 TKK528 pKa = 10.47 LVSVEE533 pKa = 3.76 KK534 pKa = 10.93 AEE536 pKa = 4.48 EE537 pKa = 4.14 KK538 pKa = 10.74 FKK540 pKa = 10.52 TGQPFGRR547 pKa = 11.84 AVMMLDD553 pKa = 3.64 ALEE556 pKa = 4.0 QAAASPLYY564 pKa = 10.4 NVLSHH569 pKa = 5.37 YY570 pKa = 9.11 TFEE573 pKa = 4.01 RR574 pKa = 11.84 RR575 pKa = 11.84 LRR577 pKa = 11.84 RR578 pKa = 11.84 DD579 pKa = 3.25 CGFKK583 pKa = 10.55 NAVIRR588 pKa = 11.84 ASSDD592 pKa = 2.65 WNEE595 pKa = 3.1 IWKK598 pKa = 9.95 GVRR601 pKa = 11.84 EE602 pKa = 4.11 AEE604 pKa = 5.06 AIIEE608 pKa = 4.16 LDD610 pKa = 2.75 WSKK613 pKa = 10.98 FDD615 pKa = 3.97 RR616 pKa = 11.84 EE617 pKa = 4.2 RR618 pKa = 11.84 PAEE621 pKa = 4.18 DD622 pKa = 3.94 LEE624 pKa = 4.88 FIVDD628 pKa = 3.7 VVISCFKK635 pKa = 10.07 PRR637 pKa = 11.84 NEE639 pKa = 3.86 RR640 pKa = 11.84 EE641 pKa = 3.75 RR642 pKa = 11.84 RR643 pKa = 11.84 LLRR646 pKa = 11.84 AYY648 pKa = 10.34 GIMMRR653 pKa = 11.84 RR654 pKa = 11.84 ALIEE658 pKa = 4.04 RR659 pKa = 11.84 LLVMDD664 pKa = 4.42 GGGVFGIEE672 pKa = 3.97 GMVPSGSLWTGWIDD686 pKa = 3.17 TALNVLYY693 pKa = 10.56 LRR695 pKa = 11.84 SACLEE700 pKa = 3.91 VGLPSQRR707 pKa = 11.84 YY708 pKa = 8.54 LPMCAGDD715 pKa = 4.88 DD716 pKa = 3.76 NLTLFWSDD724 pKa = 3.06 PGEE727 pKa = 4.2 VKK729 pKa = 10.5 LLKK732 pKa = 10.05 IRR734 pKa = 11.84 EE735 pKa = 4.45 LLNEE739 pKa = 4.6 WYY741 pKa = 10.28 RR742 pKa = 11.84 AGIDD746 pKa = 3.45 DD747 pKa = 4.78 ADD749 pKa = 4.24 FFIHH753 pKa = 6.76 RR754 pKa = 11.84 PPYY757 pKa = 10.21 HH758 pKa = 5.19 VVKK761 pKa = 10.43 RR762 pKa = 11.84 QACFPPGTDD771 pKa = 3.32 LKK773 pKa = 10.97 GGTSMLMKK781 pKa = 10.1 DD782 pKa = 3.8 AEE784 pKa = 4.23 WVEE787 pKa = 4.25 FEE789 pKa = 4.08 GEE791 pKa = 4.16 LRR793 pKa = 11.84 VDD795 pKa = 3.79 EE796 pKa = 4.49 AAGRR800 pKa = 11.84 SHH802 pKa = 7.02 RR803 pKa = 11.84 WQYY806 pKa = 10.95 LFKK809 pKa = 10.77 GRR811 pKa = 11.84 PKK813 pKa = 10.46 FLSCYY818 pKa = 4.9 WTRR821 pKa = 11.84 DD822 pKa = 3.37 GLPIRR827 pKa = 11.84 PTSDD831 pKa = 3.16 NLEE834 pKa = 4.07 KK835 pKa = 10.94 LLWPEE840 pKa = 5.26 GIHH843 pKa = 7.21 KK844 pKa = 10.88 SIDD847 pKa = 3.44 DD848 pKa = 3.97 YY849 pKa = 10.88 EE850 pKa = 5.84 ASVASMAVDD859 pKa = 3.42 NPFNHH864 pKa = 6.72 HH865 pKa = 6.04 NVNHH869 pKa = 5.88 MLMRR873 pKa = 11.84 YY874 pKa = 9.69 VIIQQIRR881 pKa = 11.84 RR882 pKa = 11.84 VSAGILSPEE891 pKa = 3.98 EE892 pKa = 3.81 CLAFCKK898 pKa = 10.2 FRR900 pKa = 11.84 SQEE903 pKa = 4.08 GEE905 pKa = 4.12 EE906 pKa = 4.03 IPYY909 pKa = 10.62 PMVAPWRR916 pKa = 11.84 RR917 pKa = 11.84 GMHH920 pKa = 5.5 EE921 pKa = 4.78 ARR923 pKa = 11.84 MEE925 pKa = 4.71 DD926 pKa = 3.84 YY927 pKa = 10.88 PEE929 pKa = 4.01 TKK931 pKa = 9.62 PWVKK935 pKa = 10.21 EE936 pKa = 3.62 FRR938 pKa = 11.84 DD939 pKa = 4.82 FISGVSSLYY948 pKa = 10.43 ARR950 pKa = 11.84 KK951 pKa = 8.27 PTGGVDD957 pKa = 3.51 AYY959 pKa = 11.16 RR960 pKa = 11.84 FMEE963 pKa = 4.95 IIRR966 pKa = 11.84 GEE968 pKa = 3.96 QSPGEE973 pKa = 4.12 GQFGSDD979 pKa = 3.12 LVKK982 pKa = 10.35 WVDD985 pKa = 3.39 WLRR988 pKa = 11.84 KK989 pKa = 9.53 HH990 pKa = 6.28 PVTKK994 pKa = 10.56 FLRR997 pKa = 11.84 ATGGRR1002 pKa = 11.84 HH1003 pKa = 4.68 HH1004 pKa = 6.68 QKK1006 pKa = 8.83 STARR1010 pKa = 11.84 LEE1012 pKa = 4.57 GEE1014 pKa = 4.39 DD1015 pKa = 3.6 LRR1017 pKa = 11.84 KK1018 pKa = 10.37 VEE1020 pKa = 4.4 DD1021 pKa = 3.21 AFMALRR1027 pKa = 11.84 EE1028 pKa = 4.14 RR1029 pKa = 11.84 LGSGQVGSTEE1039 pKa = 5.05 DD1040 pKa = 3.47 FSLWVSDD1047 pKa = 3.9 LLRR1050 pKa = 11.84 SGMM1053 pKa = 4.04
Molecular weight: 120.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.774
IPC2_protein 8.653
IPC_protein 8.58
Toseland 9.297
ProMoST 9.107
Dawson 9.589
Bjellqvist 9.341
Wikipedia 9.765
Rodwell 9.809
Grimsley 9.663
Solomon 9.619
Lehninger 9.575
Nozaki 9.443
DTASelect 9.297
Thurlkill 9.443
EMBOSS 9.736
Sillero 9.545
Patrickios 5.092
IPC_peptide 9.604
IPC2_peptide 7.936
IPC2.peptide.svr19 7.75
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1440
387
1053
720.0
82.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.917 ± 1.141
0.694 ± 0.367
5.0 ± 0.089
9.236 ± 1.126
5.208 ± 0.115
8.542 ± 0.129
1.667 ± 0.061
4.028 ± 0.353
6.597 ± 1.019
8.542 ± 1.372
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.403 ± 0.159
2.778 ± 0.034
4.514 ± 0.337
3.958 ± 0.639
9.375 ± 0.72
5.903 ± 0.115
3.611 ± 0.549
4.514 ± 0.746
1.528 ± 0.67
2.986 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here