Synechococcus phage S-H1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3G6VAE0|A0A3G6VAE0_9CAUD Uncharacterized protein OS=Synechococcus phage S-H1 OX=2488632 PE=4 SV=1
MM1 pKa = 7.48AFYY4 pKa = 10.25RR5 pKa = 11.84GEE7 pKa = 4.07EE8 pKa = 4.35GSVKK12 pKa = 10.2FDD14 pKa = 3.91DD15 pKa = 4.76AGASASAITSTRR27 pKa = 11.84SWNLTLDD34 pKa = 3.64KK35 pKa = 11.01AVLEE39 pKa = 4.39TTSMGDD45 pKa = 3.12SYY47 pKa = 11.59AGNIGSIISGSGSCEE62 pKa = 3.89VIYY65 pKa = 9.25TASSADD71 pKa = 3.33EE72 pKa = 3.73TAAFVDD78 pKa = 5.09HH79 pKa = 7.1INTAADD85 pKa = 3.67DD86 pKa = 4.01GTALFEE92 pKa = 5.36LYY94 pKa = 10.7LDD96 pKa = 3.8TSGSKK101 pKa = 10.38SISFDD106 pKa = 3.41GVVTSAEE113 pKa = 3.98YY114 pKa = 9.11TATVGEE120 pKa = 4.54LEE122 pKa = 4.6IITINFVTNGTITTTLL138 pKa = 3.31

Molecular weight:
14.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3G6VA93|A0A3G6VA93_9CAUD Uncharacterized protein OS=Synechococcus phage S-H1 OX=2488632 PE=4 SV=1
MM1 pKa = 6.82VASGKK6 pKa = 9.31SANWSYY12 pKa = 11.77LLIRR16 pKa = 11.84LIRR19 pKa = 11.84IEE21 pKa = 4.13RR22 pKa = 11.84DD23 pKa = 2.96LRR25 pKa = 11.84RR26 pKa = 11.84PPPIGLRR33 pKa = 11.84RR34 pKa = 11.84YY35 pKa = 9.27PEE37 pKa = 3.98HH38 pKa = 6.34TLATSMEE45 pKa = 4.29TDD47 pKa = 3.85FLTTEE52 pKa = 3.8QLAARR57 pKa = 11.84LGLKK61 pKa = 9.66PATIKK66 pKa = 9.64GWRR69 pKa = 11.84YY70 pKa = 9.95RR71 pKa = 11.84GIGPEE76 pKa = 3.53WHH78 pKa = 6.49EE79 pKa = 4.19LPRR82 pKa = 11.84IGRR85 pKa = 11.84SPRR88 pKa = 11.84QSRR91 pKa = 11.84VRR93 pKa = 11.84YY94 pKa = 9.36RR95 pKa = 11.84LEE97 pKa = 3.91DD98 pKa = 3.42VKK100 pKa = 10.66RR101 pKa = 11.84WEE103 pKa = 5.18SIGDD107 pKa = 3.68DD108 pKa = 3.36

Molecular weight:
12.68 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

11640

35

863

181.9

19.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.919 ± 0.403

1.555 ± 0.219

6.117 ± 0.205

5.833 ± 0.317

3.179 ± 0.154

7.895 ± 0.513

1.907 ± 0.183

5.344 ± 0.216

3.978 ± 0.227

8.531 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.959 ± 0.15

3.479 ± 0.201

4.734 ± 0.319

4.639 ± 0.237

6.418 ± 0.388

6.598 ± 0.306

6.435 ± 0.337

6.186 ± 0.331

1.521 ± 0.207

2.775 ± 0.195

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski