Synechococcus phage S-H1
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G6VAE0|A0A3G6VAE0_9CAUD Uncharacterized protein OS=Synechococcus phage S-H1 OX=2488632 PE=4 SV=1
MM1 pKa = 7.48 AFYY4 pKa = 10.25 RR5 pKa = 11.84 GEE7 pKa = 4.07 EE8 pKa = 4.35 GSVKK12 pKa = 10.2 FDD14 pKa = 3.91 DD15 pKa = 4.76 AGASASAITSTRR27 pKa = 11.84 SWNLTLDD34 pKa = 3.64 KK35 pKa = 11.01 AVLEE39 pKa = 4.39 TTSMGDD45 pKa = 3.12 SYY47 pKa = 11.59 AGNIGSIISGSGSCEE62 pKa = 3.89 VIYY65 pKa = 9.25 TASSADD71 pKa = 3.33 EE72 pKa = 3.73 TAAFVDD78 pKa = 5.09 HH79 pKa = 7.1 INTAADD85 pKa = 3.67 DD86 pKa = 4.01 GTALFEE92 pKa = 5.36 LYY94 pKa = 10.7 LDD96 pKa = 3.8 TSGSKK101 pKa = 10.38 SISFDD106 pKa = 3.41 GVVTSAEE113 pKa = 3.98 YY114 pKa = 9.11 TATVGEE120 pKa = 4.54 LEE122 pKa = 4.6 IITINFVTNGTITTTLL138 pKa = 3.31
Molecular weight: 14.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.567
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 4.075
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.897
Patrickios 1.875
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|A0A3G6VA93|A0A3G6VA93_9CAUD Uncharacterized protein OS=Synechococcus phage S-H1 OX=2488632 PE=4 SV=1
MM1 pKa = 6.82 VASGKK6 pKa = 9.31 SANWSYY12 pKa = 11.77 LLIRR16 pKa = 11.84 LIRR19 pKa = 11.84 IEE21 pKa = 4.13 RR22 pKa = 11.84 DD23 pKa = 2.96 LRR25 pKa = 11.84 RR26 pKa = 11.84 PPPIGLRR33 pKa = 11.84 RR34 pKa = 11.84 YY35 pKa = 9.27 PEE37 pKa = 3.98 HH38 pKa = 6.34 TLATSMEE45 pKa = 4.29 TDD47 pKa = 3.85 FLTTEE52 pKa = 3.8 QLAARR57 pKa = 11.84 LGLKK61 pKa = 9.66 PATIKK66 pKa = 9.64 GWRR69 pKa = 11.84 YY70 pKa = 9.95 RR71 pKa = 11.84 GIGPEE76 pKa = 3.53 WHH78 pKa = 6.49 EE79 pKa = 4.19 LPRR82 pKa = 11.84 IGRR85 pKa = 11.84 SPRR88 pKa = 11.84 QSRR91 pKa = 11.84 VRR93 pKa = 11.84 YY94 pKa = 9.36 RR95 pKa = 11.84 LEE97 pKa = 3.91 DD98 pKa = 3.42 VKK100 pKa = 10.66 RR101 pKa = 11.84 WEE103 pKa = 5.18 SIGDD107 pKa = 3.68 DD108 pKa = 3.36
Molecular weight: 12.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.706
IPC_protein 10.76
Toseland 10.687
ProMoST 10.57
Dawson 10.804
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 10.818
Grimsley 10.862
Solomon 10.979
Lehninger 10.935
Nozaki 10.657
DTASelect 10.584
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.73
Patrickios 10.54
IPC_peptide 10.979
IPC2_peptide 9.604
IPC2.peptide.svr19 8.578
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
64
0
64
11640
35
863
181.9
19.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.919 ± 0.403
1.555 ± 0.219
6.117 ± 0.205
5.833 ± 0.317
3.179 ± 0.154
7.895 ± 0.513
1.907 ± 0.183
5.344 ± 0.216
3.978 ± 0.227
8.531 ± 0.258
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.959 ± 0.15
3.479 ± 0.201
4.734 ± 0.319
4.639 ± 0.237
6.418 ± 0.388
6.598 ± 0.306
6.435 ± 0.337
6.186 ± 0.331
1.521 ± 0.207
2.775 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here