Streptococcus phage Javan515

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AGY8|A0A4D6AGY8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan515 OX=2548229 GN=Javan515_0017 PE=4 SV=1
MM1 pKa = 7.13ATVSEE6 pKa = 4.8VISYY10 pKa = 8.69WRR12 pKa = 11.84GLADD16 pKa = 3.36TGQGYY21 pKa = 9.04DD22 pKa = 5.26ADD24 pKa = 5.1LAWGWQCADD33 pKa = 3.49VTNGTTTNFFGVTLWGNAIDD53 pKa = 5.23LLDD56 pKa = 3.77SAKK59 pKa = 10.87AQGLEE64 pKa = 4.26VIYY67 pKa = 9.61DD68 pKa = 3.63APGINPKK75 pKa = 10.66AGDD78 pKa = 3.99LFVMFTYY85 pKa = 9.14GHH87 pKa = 7.42PYY89 pKa = 9.06GHH91 pKa = 6.89TGIIIADD98 pKa = 3.49SDD100 pKa = 4.4GYY102 pKa = 9.39TIQTIEE108 pKa = 4.14QNVDD112 pKa = 2.94GYY114 pKa = 11.54SDD116 pKa = 3.84NNGDD120 pKa = 4.17GINDD124 pKa = 3.52QFQVGGPARR133 pKa = 11.84YY134 pKa = 7.58VTRR137 pKa = 11.84AFSDD141 pKa = 3.37GDD143 pKa = 3.96GYY145 pKa = 10.51IVGWIRR151 pKa = 11.84PPYY154 pKa = 10.36SDD156 pKa = 3.58TSSEE160 pKa = 4.17KK161 pKa = 10.12QSQSQAPSGFRR172 pKa = 11.84KK173 pKa = 10.24LKK175 pKa = 10.89DD176 pKa = 3.35EE177 pKa = 4.36VGTFEE182 pKa = 5.09VMVPALNVRR191 pKa = 11.84RR192 pKa = 11.84EE193 pKa = 3.77PSLNGEE199 pKa = 3.97IVACYY204 pKa = 9.54QYY206 pKa = 11.74GMTGTYY212 pKa = 10.62DD213 pKa = 3.12SVYY216 pKa = 11.08VGDD219 pKa = 4.08GYY221 pKa = 10.78IWVSYY226 pKa = 10.44VGASGMRR233 pKa = 11.84NYY235 pKa = 9.76MAVGDD240 pKa = 4.48ADD242 pKa = 3.22GDD244 pKa = 4.55YY245 pKa = 11.31NVNPYY250 pKa = 10.72CKK252 pKa = 10.02FYY254 pKa = 11.49

Molecular weight: 27.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AKM1|A0A4D6AKM1_9CAUD Terminase large subunit OS=Streptococcus phage Javan515 OX=2548229 GN=Javan515_0030 PE=3 SV=1
MM1 pKa = 7.5TGMPALEE8 pKa = 4.07VKK10 pKa = 9.9LRR12 pKa = 11.84SMSEE16 pKa = 3.93KK17 pKa = 10.26RR18 pKa = 11.84WDD20 pKa = 3.33RR21 pKa = 11.84VANKK25 pKa = 10.23NLTEE29 pKa = 4.05MFNRR33 pKa = 11.84AARR36 pKa = 11.84PPGTPIGKK44 pKa = 7.18NTKK47 pKa = 7.24RR48 pKa = 11.84HH49 pKa = 5.65KK50 pKa = 10.69SGEE53 pKa = 4.02LLRR56 pKa = 11.84SRR58 pKa = 11.84RR59 pKa = 11.84LKK61 pKa = 10.52KK62 pKa = 10.55VNSSKK67 pKa = 11.17DD68 pKa = 3.54VITGNFGYY76 pKa = 10.24IKK78 pKa = 10.19DD79 pKa = 4.04YY80 pKa = 10.93APHH83 pKa = 6.57VEE85 pKa = 4.05YY86 pKa = 10.65GHH88 pKa = 6.96RR89 pKa = 11.84IVRR92 pKa = 11.84NGKK95 pKa = 8.3QVGYY99 pKa = 11.31ANGTKK104 pKa = 10.56YY105 pKa = 10.86LFNNVKK111 pKa = 9.38KK112 pKa = 10.39QRR114 pKa = 11.84EE115 pKa = 4.3IYY117 pKa = 9.23RR118 pKa = 11.84QDD120 pKa = 3.14MLNEE124 pKa = 3.91LRR126 pKa = 11.84RR127 pKa = 3.78

Molecular weight: 14.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11371

40

1146

192.7

21.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.596 ± 0.366

0.413 ± 0.069

6.613 ± 0.178

7.018 ± 0.421

3.887 ± 0.223

6.103 ± 0.428

1.275 ± 0.127

7.203 ± 0.242

8.416 ± 0.401

8.126 ± 0.408

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.196

5.892 ± 0.217

2.92 ± 0.171

3.966 ± 0.202

4.01 ± 0.265

6.701 ± 0.467

6.499 ± 0.418

6.455 ± 0.313

1.24 ± 0.11

4.406 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski