Neorickettsia sennetsu (strain ATCC VR-367 / Miyayama) (Ehrlichia sennetsu)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Neorickettsia; Neorickettsia sennetsu

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 932 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2GDV5|Q2GDV5_NEOSM IAT_beta domain-containing protein OS=Neorickettsia sennetsu (strain ATCC VR-367 / Miyayama) OX=222891 GN=NSE_0456 PE=3 SV=1
MM1 pKa = 7.09QNEE4 pKa = 4.29AYY6 pKa = 9.21IVQGFRR12 pKa = 11.84VGFFLQAMVSNDD24 pKa = 3.2ADD26 pKa = 3.83VILYY30 pKa = 9.79

Molecular weight: 3.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2GCY5|Q2GCY5_NEOSM Uncharacterized protein OS=Neorickettsia sennetsu (strain ATCC VR-367 / Miyayama) OX=222891 GN=NSE_0786 PE=4 SV=1
MM1 pKa = 7.71ANTASAKK8 pKa = 10.45KK9 pKa = 8.76MVRR12 pKa = 11.84KK13 pKa = 9.88IKK15 pKa = 10.53RR16 pKa = 11.84RR17 pKa = 11.84TLVNKK22 pKa = 9.21MRR24 pKa = 11.84MSRR27 pKa = 11.84IRR29 pKa = 11.84TFVRR33 pKa = 11.84KK34 pKa = 8.86VRR36 pKa = 11.84KK37 pKa = 9.73AIAVGPKK44 pKa = 9.52SAAAEE49 pKa = 4.21ALRR52 pKa = 11.84VAQPEE57 pKa = 3.78IHH59 pKa = 6.73RR60 pKa = 11.84GVTKK64 pKa = 10.62GVLHH68 pKa = 6.98KK69 pKa = 10.8NRR71 pKa = 11.84AARR74 pKa = 11.84IVSRR78 pKa = 11.84LSGHH82 pKa = 5.71IKK84 pKa = 10.33KK85 pKa = 8.71MAA87 pKa = 4.09

Molecular weight: 9.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

932

0

932

250202

29

1921

268.5

29.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.94 ± 0.08

1.718 ± 0.037

4.727 ± 0.061

6.456 ± 0.086

5.019 ± 0.082

6.632 ± 0.074

2.025 ± 0.034

7.359 ± 0.078

6.657 ± 0.075

10.518 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.034

4.149 ± 0.052

3.457 ± 0.049

2.765 ± 0.045

5.03 ± 0.06

7.97 ± 0.08

4.784 ± 0.063

7.641 ± 0.08

0.701 ± 0.024

3.122 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski