Oscillibacter sp. CAG:155
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2148 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R6UAW7|R6UAW7_9FIRM HTH cro/C1-type domain-containing protein OS=Oscillibacter sp. CAG:155 OX=1262910 GN=BN503_00927 PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.38 KK3 pKa = 10.2 KK4 pKa = 10.55 LLSLVMAAAMVLGLAACGSNSDD26 pKa = 3.72 TADD29 pKa = 3.14 TSGSDD34 pKa = 3.73 DD35 pKa = 3.8 SASGTTFKK43 pKa = 10.59 IGTIGPLTGEE53 pKa = 3.81 NAIYY57 pKa = 10.57 GLAVANGAKK66 pKa = 9.9 IAVEE70 pKa = 4.56 EE71 pKa = 4.43 INASDD76 pKa = 3.68 SAIKK80 pKa = 10.59 FEE82 pKa = 4.94 LQSQDD87 pKa = 3.43 DD88 pKa = 4.14 VADD91 pKa = 4.27 GEE93 pKa = 4.83 TSVNAYY99 pKa = 9.81 NKK101 pKa = 10.51 LMDD104 pKa = 3.44 WGMQLLVGPTTTGAAQAVSAVTNTDD129 pKa = 2.91 RR130 pKa = 11.84 TFMLTPSASSTDD142 pKa = 3.8 VIDD145 pKa = 4.73 GKK147 pKa = 10.99 DD148 pKa = 3.27 NVFQVCFTDD157 pKa = 4.09 PNQGSSSADD166 pKa = 3.12 YY167 pKa = 9.31 MAEE170 pKa = 3.84 NMPDD174 pKa = 3.11 AKK176 pKa = 10.42 IAIIYY181 pKa = 10.25 RR182 pKa = 11.84 NDD184 pKa = 3.28 DD185 pKa = 3.68 AYY187 pKa = 11.59 SQGIHH192 pKa = 6.11 DD193 pKa = 4.31 TFVTEE198 pKa = 4.68 AAAKK202 pKa = 9.04 GLNVVYY208 pKa = 10.32 EE209 pKa = 4.41 GTFTKK214 pKa = 9.93 DD215 pKa = 2.43 TATDD219 pKa = 3.9 FSVQLTGAQSAGADD233 pKa = 3.57 LVFLPIYY240 pKa = 7.46 YY241 pKa = 10.04 QPASVILNQANAMGYY256 pKa = 9.92 APTFFGVDD264 pKa = 3.29 GMDD267 pKa = 5.2 GILTMEE273 pKa = 4.66 GFDD276 pKa = 3.28 TSLAEE281 pKa = 4.23 GVMLLTPFSATLEE294 pKa = 4.25 EE295 pKa = 4.25 NQSFVQAYY303 pKa = 8.89 QDD305 pKa = 3.68 EE306 pKa = 4.97 FGDD309 pKa = 4.0 TPNQFAADD317 pKa = 4.08 AYY319 pKa = 10.35 DD320 pKa = 3.21 AVYY323 pKa = 10.23 ILKK326 pKa = 10.32 AALEE330 pKa = 4.29 AAEE333 pKa = 4.81 CTPDD337 pKa = 3.41 MSSADD342 pKa = 3.22 ICEE345 pKa = 3.9 ALIPVMPTLSYY356 pKa = 11.35 DD357 pKa = 3.4 GLTGKK362 pKa = 10.7 GMTWDD367 pKa = 3.17 ASGAVSKK374 pKa = 11.11 DD375 pKa = 2.84 PTAFVIEE382 pKa = 4.28 NGTYY386 pKa = 10.39 VLPP389 pKa = 4.73
Molecular weight: 40.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.694
IPC_protein 3.732
Toseland 3.49
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.846
Patrickios 1.227
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|R6UBB9|R6UBB9_9FIRM ABC transporter permease protein OS=Oscillibacter sp. CAG:155 OX=1262910 GN=BN503_01035 PE=3 SV=1
MM1 pKa = 7.29 GAFVKK6 pKa = 10.35 KK7 pKa = 10.02 IHH9 pKa = 7.05 LSTLLSNGLWSLLGLALLLWPAPSARR35 pKa = 11.84 AVCLALGAVLLLCGAGNLAAALPNRR60 pKa = 11.84 DD61 pKa = 3.29 EE62 pKa = 4.41 TLYY65 pKa = 10.17 TALRR69 pKa = 11.84 LGSGVILAVIGLWLLFRR86 pKa = 11.84 PLLAAALIPKK96 pKa = 8.55 LTGILLCIHH105 pKa = 6.96 GGSSLGDD112 pKa = 3.2 AMLLRR117 pKa = 11.84 RR118 pKa = 11.84 GGDD121 pKa = 3.63 ARR123 pKa = 11.84 WSAAVLGGAISLALGLLLFFLAVPAFLIAARR154 pKa = 11.84 IVGACLLWDD163 pKa = 4.13 GVSGLWIASRR173 pKa = 11.84 VGHH176 pKa = 7.12 LPRR179 pKa = 11.84 QDD181 pKa = 3.1 PSGRR185 pKa = 3.58
Molecular weight: 19.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.385
IPC_protein 10.101
Toseland 10.57
ProMoST 10.804
Dawson 10.657
Bjellqvist 10.409
Wikipedia 10.862
Rodwell 10.76
Grimsley 10.687
Solomon 10.804
Lehninger 10.789
Nozaki 10.643
DTASelect 10.365
Thurlkill 10.57
EMBOSS 10.979
Sillero 10.613
Patrickios 10.599
IPC_peptide 10.818
IPC2_peptide 9.897
IPC2.peptide.svr19 8.778
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2148
0
2148
634855
29
1836
295.6
32.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.915 ± 0.055
1.711 ± 0.024
5.592 ± 0.044
6.689 ± 0.048
3.658 ± 0.033
8.156 ± 0.053
1.929 ± 0.028
5.388 ± 0.042
4.324 ± 0.053
10.219 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.741 ± 0.027
3.012 ± 0.033
4.23 ± 0.034
3.632 ± 0.041
5.739 ± 0.057
5.442 ± 0.038
5.802 ± 0.042
7.527 ± 0.057
1.023 ± 0.021
3.267 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here