Primnoa pacifica coral associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RL89|A0A0K1RL89_9CIRC Replication-associated protein OS=Primnoa pacifica coral associated circular virus OX=1692262 PE=4 SV=1
MM1 pKa = 7.57DD2 pKa = 5.71TINGRR7 pKa = 11.84TASMSCPARR16 pKa = 11.84CITRR20 pKa = 11.84RR21 pKa = 11.84VLYY24 pKa = 10.71SEE26 pKa = 4.61GANSVAVADD35 pKa = 5.47EE36 pKa = 4.31IPITTTTKK44 pKa = 10.13FPWMSLEE51 pKa = 4.14RR52 pKa = 11.84DD53 pKa = 3.57LSKK56 pKa = 10.88LPQCGEE62 pKa = 4.11VNTFIPMVVIRR73 pKa = 11.84LDD75 pKa = 3.7SNTNLPTTSTILEE88 pKa = 4.81DD89 pKa = 3.77PQTWKK94 pKa = 10.41PFLRR98 pKa = 11.84EE99 pKa = 3.84QIQKK103 pKa = 10.56FGWFYY108 pKa = 10.52RR109 pKa = 11.84ANVSWSVRR117 pKa = 11.84GKK119 pKa = 10.04HH120 pKa = 6.35LEE122 pKa = 3.95AQLAVDD128 pKa = 3.91PAVAPEE134 pKa = 5.09IITIDD139 pKa = 3.23

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RL89|A0A0K1RL89_9CIRC Replication-associated protein OS=Primnoa pacifica coral associated circular virus OX=1692262 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 9.56GTIAQNLEE10 pKa = 4.19YY11 pKa = 9.64TGKK14 pKa = 10.18EE15 pKa = 3.74ATEE18 pKa = 4.85DD19 pKa = 3.46PTKK22 pKa = 10.12LHH24 pKa = 5.88EE25 pKa = 4.7WGVKK29 pKa = 9.64PLTNTEE35 pKa = 4.15RR36 pKa = 11.84NKK38 pKa = 10.66KK39 pKa = 8.88GAAASKK45 pKa = 8.7EE46 pKa = 4.31TMKK49 pKa = 10.8EE50 pKa = 3.74VIAAAEE56 pKa = 4.15RR57 pKa = 11.84GDD59 pKa = 3.97LEE61 pKa = 4.78WIKK64 pKa = 10.79SEE66 pKa = 4.43HH67 pKa = 6.32PGHH70 pKa = 6.16YY71 pKa = 9.49LRR73 pKa = 11.84YY74 pKa = 7.96MKK76 pKa = 10.37QIEE79 pKa = 4.58KK80 pKa = 10.37VRR82 pKa = 11.84MLALVDD88 pKa = 3.64QTTEE92 pKa = 3.81THH94 pKa = 5.86STLEE98 pKa = 4.27NYY100 pKa = 8.06WIHH103 pKa = 7.03GDD105 pKa = 3.39TGSGKK110 pKa = 7.95TRR112 pKa = 11.84AVRR115 pKa = 11.84EE116 pKa = 4.17KK117 pKa = 10.64IPDD120 pKa = 3.26KK121 pKa = 10.99DD122 pKa = 4.38LYY124 pKa = 11.55LKK126 pKa = 10.23GQNKK130 pKa = 7.82WWDD133 pKa = 3.31HH134 pKa = 5.35YY135 pKa = 11.11EE136 pKa = 3.99GEE138 pKa = 4.56KK139 pKa = 10.79YY140 pKa = 10.98VLIDD144 pKa = 4.38DD145 pKa = 5.09YY146 pKa = 11.7SPEE149 pKa = 3.8WAEE152 pKa = 5.74KK153 pKa = 10.85YY154 pKa = 10.09FLKK157 pKa = 10.68NWADD161 pKa = 3.51HH162 pKa = 6.05YY163 pKa = 11.42SFSAEE168 pKa = 4.04YY169 pKa = 10.36KK170 pKa = 9.85GGSLKK175 pKa = 10.6RR176 pKa = 11.84LNPKK180 pKa = 9.25TIIVTSNYY188 pKa = 9.74KK189 pKa = 9.9IEE191 pKa = 3.9EE192 pKa = 4.08AGFNEE197 pKa = 4.57KK198 pKa = 10.46DD199 pKa = 3.22IEE201 pKa = 4.09PMMRR205 pKa = 11.84RR206 pKa = 11.84FKK208 pKa = 10.71RR209 pKa = 11.84GNAQWFIDD217 pKa = 3.88TFKK220 pKa = 11.48VEE222 pKa = 3.92

Molecular weight: 25.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

361

139

222

180.5

20.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.925 ± 0.139

0.831 ± 0.687

5.263 ± 0.118

8.864 ± 1.609

3.047 ± 0.285

5.54 ± 1.006

2.216 ± 0.775

6.371 ± 0.798

8.587 ± 2.582

6.648 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.056

4.432 ± 0.06

4.709 ± 1.286

2.77 ± 0.428

5.263 ± 0.627

5.263 ± 0.999

8.31 ± 0.912

4.986 ± 1.143

3.324 ± 0.231

3.878 ± 1.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski