Sida golden yellow vein virus-[A11]
Average proteome isoelectric point is 7.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9YNW3|Q9YNW3_9GEMI Capsid protein OS=Sida golden yellow vein virus-[A11] OX=223318 GN=AR1 PE=3 SV=1
MM1 pKa = 7.45 IVNLPEE7 pKa = 5.37 SPDD10 pKa = 3.39 QSLLVAGILTPSDD23 pKa = 3.45 FTIEE27 pKa = 4.04 PVHH30 pKa = 6.62 NLITITEE37 pKa = 4.09 IVLNRR42 pKa = 11.84 GGARR46 pKa = 11.84 LVVIHH51 pKa = 6.24 VKK53 pKa = 10.41 HH54 pKa = 5.8 LAKK57 pKa = 10.58 VHH59 pKa = 5.92 WSAIGSTIPDD69 pKa = 3.5 QPEE72 pKa = 4.34 HH73 pKa = 5.05 YY74 pKa = 8.47 TVRR77 pKa = 11.84 VVLEE81 pKa = 4.49 LDD83 pKa = 3.81 VLIHH87 pKa = 6.74 PNLPP91 pKa = 3.52
Molecular weight: 10.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.664
IPC2_protein 5.486
IPC_protein 5.486
Toseland 6.059
ProMoST 5.855
Dawson 5.766
Bjellqvist 5.766
Wikipedia 5.792
Rodwell 5.753
Grimsley 6.3
Solomon 5.766
Lehninger 5.766
Nozaki 6.033
DTASelect 6.237
Thurlkill 6.249
EMBOSS 6.211
Sillero 6.148
Patrickios 4.202
IPC_peptide 5.779
IPC2_peptide 6.173
IPC2.peptide.svr19 6.064
Protein with the highest isoelectric point:
>tr|Q9YNW3|Q9YNW3_9GEMI Capsid protein OS=Sida golden yellow vein virus-[A11] OX=223318 GN=AR1 PE=3 SV=1
MM1 pKa = 7.52 TKK3 pKa = 9.87 RR4 pKa = 11.84 DD5 pKa = 3.7 SSWRR9 pKa = 11.84 SMAGTTKK16 pKa = 9.91 VRR18 pKa = 11.84 RR19 pKa = 11.84 TLNFSPRR26 pKa = 11.84 GGGGPKK32 pKa = 7.64 QTRR35 pKa = 11.84 ASEE38 pKa = 4.1 WVNRR42 pKa = 11.84 PMYY45 pKa = 9.91 RR46 pKa = 11.84 KK47 pKa = 8.86 PLIYY51 pKa = 9.17 RR52 pKa = 11.84 TLRR55 pKa = 11.84 TPDD58 pKa = 3.21 VPRR61 pKa = 11.84 GCEE64 pKa = 4.34 GPCKK68 pKa = 9.32 VQSYY72 pKa = 6.94 EE73 pKa = 3.74 QRR75 pKa = 11.84 HH76 pKa = 6.38 DD77 pKa = 3.16 ISHH80 pKa = 6.13 VGKK83 pKa = 10.55 VMCISDD89 pKa = 3.56 VTRR92 pKa = 11.84 GNGITHH98 pKa = 6.78 RR99 pKa = 11.84 VGKK102 pKa = 9.44 RR103 pKa = 11.84 FCVKK107 pKa = 10.05 SVYY110 pKa = 9.82 TLGKK114 pKa = 9.18 IWMDD118 pKa = 3.36 EE119 pKa = 4.0 NIKK122 pKa = 10.61 LKK124 pKa = 10.73 NHH126 pKa = 5.95 TNSVMFWLVRR136 pKa = 11.84 DD137 pKa = 3.7 RR138 pKa = 11.84 RR139 pKa = 11.84 PYY141 pKa = 8.05 GTPMDD146 pKa = 4.6 FGQVFNMYY154 pKa = 10.66 DD155 pKa = 3.86 NEE157 pKa = 4.36 PSTATIKK164 pKa = 10.89 NDD166 pKa = 3.0 LRR168 pKa = 11.84 DD169 pKa = 3.83 RR170 pKa = 11.84 YY171 pKa = 10.16 QVMHH175 pKa = 6.95 RR176 pKa = 11.84 FYY178 pKa = 11.43 GKK180 pKa = 8.6 VTGGQYY186 pKa = 11.1 ASNEE190 pKa = 3.84 QALVRR195 pKa = 11.84 RR196 pKa = 11.84 FWKK199 pKa = 10.36 INNHH203 pKa = 4.29 VVYY206 pKa = 10.5 NHH208 pKa = 5.8 QEE210 pKa = 3.47 AGKK213 pKa = 10.41 YY214 pKa = 8.39 EE215 pKa = 4.09 NHH217 pKa = 6.52 TEE219 pKa = 3.99 NALLLYY225 pKa = 7.29 MACTHH230 pKa = 7.07 ASNPVYY236 pKa = 9.86 ATLKK240 pKa = 9.47 IRR242 pKa = 11.84 IYY244 pKa = 10.59 FYY246 pKa = 11.38 DD247 pKa = 4.2 SITNN251 pKa = 3.6
Molecular weight: 29.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.177
IPC2_protein 9.487
IPC_protein 9.706
Toseland 9.926
ProMoST 9.736
Dawson 10.204
Bjellqvist 9.94
Wikipedia 10.409
Rodwell 10.467
Grimsley 10.292
Solomon 10.218
Lehninger 10.175
Nozaki 9.984
DTASelect 9.911
Thurlkill 10.028
EMBOSS 10.365
Sillero 10.116
Patrickios 7.556
IPC_peptide 10.218
IPC2_peptide 8.843
IPC2.peptide.svr19 8.469
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1048
85
360
174.7
19.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.725 ± 0.916
1.622 ± 0.376
4.771 ± 0.657
5.439 ± 0.724
3.435 ± 0.575
5.821 ± 0.521
4.008 ± 0.514
6.202 ± 0.759
5.534 ± 1.078
7.729 ± 0.802
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.29 ± 0.566
5.725 ± 0.787
5.63 ± 0.864
4.198 ± 0.632
6.87 ± 1.447
7.729 ± 1.122
5.439 ± 0.865
6.011 ± 1.133
1.813 ± 0.09
4.008 ± 0.745
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here