Plasmodium ovale (malaria parasite P. ovale)
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6625 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D3TGC1|A0A1D3TGC1_9APIC Transcription elongation factor SPT4 putative OS=Plasmodium ovale OX=36330 GN=SPT4 PE=3 SV=1
MM1 pKa = 6.48 NTHH4 pKa = 6.75 VILSTEE10 pKa = 3.95 EE11 pKa = 3.84 GGIVYY16 pKa = 10.08 QATDD20 pKa = 3.42 SRR22 pKa = 11.84 DD23 pKa = 3.59 LTDD26 pKa = 3.42 MSEE29 pKa = 4.06 PTDD32 pKa = 3.88 LGEE35 pKa = 4.55 SADD38 pKa = 4.08 LGEE41 pKa = 4.46 LTDD44 pKa = 3.57 VGEE47 pKa = 4.24 VTEE50 pKa = 4.15 VGEE53 pKa = 4.1 MTEE56 pKa = 3.74 ASEE59 pKa = 4.32 VIEE62 pKa = 4.51 VGDD65 pKa = 3.58 ATEE68 pKa = 4.05 PTEE71 pKa = 4.05 VGDD74 pKa = 3.58 IGEE77 pKa = 3.98 IAYY80 pKa = 10.15 ASEE83 pKa = 3.94 IGHH86 pKa = 5.95 VGEE89 pKa = 5.07 IGHH92 pKa = 6.81 AGEE95 pKa = 4.81 IGHH98 pKa = 6.92 AGEE101 pKa = 4.81 IGHH104 pKa = 6.95 AGEE107 pKa = 4.08 IGYY110 pKa = 10.53 VGDD113 pKa = 3.23 IAYY116 pKa = 9.94 AHH118 pKa = 7.38 DD119 pKa = 3.51 IAYY122 pKa = 10.49 VDD124 pKa = 5.24 DD125 pKa = 4.08 IAYY128 pKa = 10.41 ADD130 pKa = 5.4 DD131 pKa = 3.89 IAHH134 pKa = 7.28 ADD136 pKa = 4.47 DD137 pKa = 4.39 IAHH140 pKa = 7.27 ADD142 pKa = 4.11 DD143 pKa = 3.72 IAYY146 pKa = 10.75 SGDD149 pKa = 3.1 ISNADD154 pKa = 3.81 EE155 pKa = 3.84 IAYY158 pKa = 8.26 TSNVGYY164 pKa = 10.07 ADD166 pKa = 3.34 NMGYY170 pKa = 8.22 TEE172 pKa = 4.24 NLSYY176 pKa = 10.11 TNNMDD181 pKa = 4.11 HH182 pKa = 7.28 IGDD185 pKa = 3.43 EE186 pKa = 4.14 RR187 pKa = 11.84 YY188 pKa = 8.19 YY189 pKa = 10.47 TFNVDD194 pKa = 3.16 YY195 pKa = 10.66 TGDD198 pKa = 3.07 QRR200 pKa = 11.84 YY201 pKa = 6.25 TVNVDD206 pKa = 3.12 YY207 pKa = 11.08 TGDD210 pKa = 3.52 TRR212 pKa = 11.84 HH213 pKa = 5.88 VRR215 pKa = 11.84 DD216 pKa = 3.42 TRR218 pKa = 11.84 YY219 pKa = 8.73 TGDD222 pKa = 3.23 TRR224 pKa = 11.84 CTVNVGYY231 pKa = 8.58 TRR233 pKa = 11.84 NAGYY237 pKa = 8.66 TGDD240 pKa = 3.55 EE241 pKa = 4.9 GYY243 pKa = 9.09 TINVGYY249 pKa = 10.65 VGDD252 pKa = 3.86 AGYY255 pKa = 8.11 TVNVGYY261 pKa = 10.51 RR262 pKa = 11.84 GDD264 pKa = 3.16 ARR266 pKa = 11.84 YY267 pKa = 7.33 TVNVDD272 pKa = 3.17 YY273 pKa = 10.55 TVDD276 pKa = 3.53 ADD278 pKa = 3.7 HH279 pKa = 7.04 TGGARR284 pKa = 11.84 YY285 pKa = 7.25 TLNVGYY291 pKa = 8.58 TGDD294 pKa = 3.26 GGYY297 pKa = 8.35 TRR299 pKa = 11.84 NSDD302 pKa = 3.26 YY303 pKa = 10.83 RR304 pKa = 11.84 RR305 pKa = 11.84 NSGYY309 pKa = 8.7 TRR311 pKa = 11.84 NARR314 pKa = 11.84 YY315 pKa = 10.18 SGDD318 pKa = 3.44 VRR320 pKa = 11.84 EE321 pKa = 4.41 MATLTTASDD330 pKa = 4.31 WIEE333 pKa = 3.84 MADD336 pKa = 3.63 VVNSGGMTEE345 pKa = 3.87 MGEE348 pKa = 4.18 VAEE351 pKa = 4.4 EE352 pKa = 4.46 TEE354 pKa = 4.27 GTDD357 pKa = 3.55 VIEE360 pKa = 4.64 SNHH363 pKa = 4.86 VAEE366 pKa = 4.65 VTDD369 pKa = 3.79 VTQLSDD375 pKa = 3.25 TTYY378 pKa = 9.48 DD379 pKa = 3.27 TDD381 pKa = 3.48 VTEE384 pKa = 5.2 VIDD387 pKa = 3.81 ATRR390 pKa = 11.84 LTHH393 pKa = 5.85 EE394 pKa = 4.45 TDD396 pKa = 3.2 VTQLSDD402 pKa = 3.66 DD403 pKa = 3.89 TDD405 pKa = 3.71 VTDD408 pKa = 3.43 VTEE411 pKa = 5.1 VIDD414 pKa = 3.72 ATQLSDD420 pKa = 3.26 ATEE423 pKa = 4.09 ATQFSDD429 pKa = 4.31 VIEE432 pKa = 4.48 EE433 pKa = 4.32 NPSHH437 pKa = 7.32 FSLL440 pKa = 5.2
Molecular weight: 47.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.789
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.126
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.91
Patrickios 0.375
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A1D3TIQ4|A0A1D3TIQ4_9APIC Uncharacterized protein OS=Plasmodium ovale OX=36330 GN=PocGH01_11012700 PE=4 SV=1
MM1 pKa = 7.28 AHH3 pKa = 6.31 GASRR7 pKa = 11.84 YY8 pKa = 8.75 KK9 pKa = 10.48 KK10 pKa = 10.3 SRR12 pKa = 11.84 AKK14 pKa = 9.88 MRR16 pKa = 11.84 WKK18 pKa = 9.16 WKK20 pKa = 9.29 KK21 pKa = 9.72 KK22 pKa = 6.67 RR23 pKa = 11.84 TRR25 pKa = 11.84 RR26 pKa = 11.84 LQKK29 pKa = 9.69 KK30 pKa = 7.67 RR31 pKa = 11.84 RR32 pKa = 11.84 KK33 pKa = 8.24 MRR35 pKa = 11.84 QRR37 pKa = 11.84 SRR39 pKa = 3.22
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.486
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.193
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6625
0
6625
4221578
29
9915
637.2
73.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.14 ± 0.023
2.078 ± 0.013
5.733 ± 0.023
7.922 ± 0.035
4.675 ± 0.025
4.739 ± 0.033
2.396 ± 0.012
7.586 ± 0.03
10.497 ± 0.038
7.939 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.125 ± 0.009
9.423 ± 0.041
2.423 ± 0.019
2.658 ± 0.015
4.019 ± 0.021
7.784 ± 0.03
4.534 ± 0.017
4.713 ± 0.019
0.623 ± 0.007
4.988 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here