Gordonia phage RogerDodger

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wizardvirus; unclassified Wizardvirus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A515MHN5|A0A515MHN5_9CAUD Uncharacterized protein OS=Gordonia phage RogerDodger OX=2591116 GN=2 PE=4 SV=1
MM1 pKa = 7.26IRR3 pKa = 11.84KK4 pKa = 9.62LSTALATLAILAAPVAVAMANPTPAPAQVPAPCVIQLGNGGTAPCPPPIVSTDD57 pKa = 3.04GSSIGGGANTDD68 pKa = 3.75PVNMGDD74 pKa = 4.63LPRR77 pKa = 11.84TGPDD81 pKa = 3.25YY82 pKa = 11.07SYY84 pKa = 10.78EE85 pKa = 4.21APTWTPDD92 pKa = 3.15EE93 pKa = 4.69EE94 pKa = 4.7EE95 pKa = 5.07PDD97 pKa = 3.7TEE99 pKa = 4.46EE100 pKa = 3.96PTEE103 pKa = 4.21TEE105 pKa = 4.06EE106 pKa = 4.15PAPEE110 pKa = 4.82AEE112 pKa = 4.19

Molecular weight: 11.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A515MHT8|A0A515MHT8_9CAUD Site-specific DNA-methyltransferase (cytosine-N(4)-specific) OS=Gordonia phage RogerDodger OX=2591116 GN=62 PE=3 SV=1
MM1 pKa = 7.78HH2 pKa = 7.55DD3 pKa = 3.97HH4 pKa = 6.19ATKK7 pKa = 9.97TLSASAASTEE17 pKa = 3.98SGIPKK22 pKa = 8.89RR23 pKa = 11.84TILYY27 pKa = 9.85AIQTRR32 pKa = 11.84KK33 pKa = 9.7LRR35 pKa = 11.84AEE37 pKa = 4.15KK38 pKa = 10.64LPGAGYY44 pKa = 9.75VIRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84DD50 pKa = 3.51FEE52 pKa = 3.82RR53 pKa = 11.84WRR55 pKa = 11.84EE56 pKa = 3.86SRR58 pKa = 11.84EE59 pKa = 3.91QVASS63 pKa = 3.62

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

18908

29

1837

194.9

21.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.355 ± 0.499

0.82 ± 0.113

7.209 ± 0.274

5.553 ± 0.358

2.449 ± 0.172

8.578 ± 0.45

2.047 ± 0.188

4.173 ± 0.174

2.845 ± 0.179

8.087 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.041 ± 0.123

2.782 ± 0.167

6.235 ± 0.347

3.501 ± 0.154

7.415 ± 0.388

5.215 ± 0.223

7.066 ± 0.346

7.288 ± 0.187

2.264 ± 0.131

2.078 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski