Phytophthora nicotianae (Buckeye rot agent)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Phytophthora

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15887 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0W8C4X9|A0A0W8C4X9_PHYNI Uncharacterized protein OS=Phytophthora nicotianae OX=4790 GN=AM587_10000106 PE=4 SV=1
MM1 pKa = 7.65LSRR4 pKa = 11.84YY5 pKa = 8.89AVQLALIAASIAVFSSSSDD24 pKa = 3.06ACLTGLFTGNDD35 pKa = 3.94CDD37 pKa = 4.77SDD39 pKa = 3.98STSISLLGGTVDD51 pKa = 4.5APCDD55 pKa = 3.64EE56 pKa = 4.87DD57 pKa = 4.08TPSILSGGLLGGGLTGGLSGGITADD82 pKa = 4.07APCDD86 pKa = 3.95DD87 pKa = 4.9DD88 pKa = 3.92TPSIVSSGLTSGLTSGLSSTGLSGVTADD116 pKa = 4.67IPCDD120 pKa = 3.59DD121 pKa = 4.29TPSVISSGLPSTGASTGVSTSTGTQQASATNSGTSATASIPSALTSSSPSTATGASTTASGTATSTATATSTAQGTMTSSAVDD204 pKa = 3.67PSTAAQTSTAADD216 pKa = 3.92PATKK220 pKa = 10.19AGVSSAVSTDD230 pKa = 3.2PVTKK234 pKa = 10.3SGTSASTTLNSAAQHH249 pKa = 5.76LRR251 pKa = 11.84HH252 pKa = 6.1

Molecular weight:
23.72 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0W8CN50|A0A0W8CN50_PHYNI HEAT repeat-containing protein 5B OS=Phytophthora nicotianae OX=4790 GN=AM587_10010541 PE=4 SV=1
MM1 pKa = 7.38VNDD4 pKa = 4.39GARR7 pKa = 11.84RR8 pKa = 11.84AKK10 pKa = 9.98KK11 pKa = 10.18RR12 pKa = 11.84PLPLPLAPVVPIAARR27 pKa = 11.84VSTNHH32 pKa = 6.3KK33 pKa = 10.45DD34 pKa = 3.54AVRR37 pKa = 11.84TNASSNEE44 pKa = 3.85YY45 pKa = 9.88RR46 pKa = 11.84SSSAKK51 pKa = 9.44AHH53 pKa = 5.67EE54 pKa = 4.59RR55 pKa = 11.84NLAKK59 pKa = 10.51LQLHH63 pKa = 6.68LPRR66 pKa = 11.84DD67 pKa = 3.87VVFFDD72 pKa = 3.19PRR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84VYY78 pKa = 10.39HH79 pKa = 5.0SHH81 pKa = 6.36VDD83 pKa = 3.5LSGWRR88 pKa = 11.84VTDD91 pKa = 4.22ASLQQLQLNNDD102 pKa = 3.46RR103 pKa = 11.84DD104 pKa = 4.3DD105 pKa = 4.62NPVKK109 pKa = 10.74SSFHH113 pKa = 6.15TLTVDD118 pKa = 3.8GSAHH122 pKa = 5.61ISARR126 pKa = 11.84GLLLMLEE133 pKa = 4.41VGTAGGGMFRR143 pKa = 11.84ALRR146 pKa = 11.84LPHH149 pKa = 6.39CQLLSRR155 pKa = 11.84GGGEE159 pKa = 3.89ILDD162 pKa = 4.35GGSLPRR168 pKa = 11.84SLTEE172 pKa = 4.48LDD174 pKa = 3.57VSSCEE179 pKa = 4.04WVDD182 pKa = 4.17DD183 pKa = 3.94KK184 pKa = 11.32FLRR187 pKa = 11.84TLARR191 pKa = 11.84HH192 pKa = 5.97CSVLAQVTLAHH203 pKa = 6.58CRR205 pKa = 11.84RR206 pKa = 11.84VTDD209 pKa = 3.36YY210 pKa = 11.2GVAAFGEE217 pKa = 5.03SYY219 pKa = 10.66AASLTSLDD227 pKa = 3.59VSFCTKK233 pKa = 9.82LTDD236 pKa = 3.52TALLALLVGSSSSPAVPGGAQMATLSASARR266 pKa = 11.84IRR268 pKa = 11.84VLNVAGLPLVDD279 pKa = 4.58GLTLLGLRR287 pKa = 11.84GPCASRR293 pKa = 11.84LEE295 pKa = 4.1VLNMSGCTVLRR306 pKa = 11.84VAALQRR312 pKa = 11.84LAQVRR317 pKa = 11.84ALIRR321 pKa = 11.84LTKK324 pKa = 10.26LDD326 pKa = 4.09LSRR329 pKa = 11.84CSLVDD334 pKa = 3.81DD335 pKa = 5.32QILTALGLACPKK347 pKa = 10.51LAMLLLAFCSNITDD361 pKa = 3.33VGIRR365 pKa = 11.84RR366 pKa = 11.84LVGEE370 pKa = 4.17TAIVRR375 pKa = 11.84ADD377 pKa = 3.5QNDD380 pKa = 4.48GAGHH384 pKa = 6.62EE385 pKa = 4.26PTIEE389 pKa = 4.12DD390 pKa = 3.45QEE392 pKa = 4.72DD393 pKa = 3.58FGLEE397 pKa = 3.94NTSRR401 pKa = 11.84AIPRR405 pKa = 11.84NGEE408 pKa = 3.85SGCRR412 pKa = 11.84HH413 pKa = 6.89LEE415 pKa = 3.93TLDD418 pKa = 3.13ITGCFQVTTRR428 pKa = 11.84GISALGIRR436 pKa = 11.84CPQLRR441 pKa = 11.84SVTLDD446 pKa = 3.02GVRR449 pKa = 11.84RR450 pKa = 11.84LDD452 pKa = 3.39SSGIRR457 pKa = 11.84ALLHH461 pKa = 5.66GCRR464 pKa = 11.84KK465 pKa = 9.61LRR467 pKa = 11.84TIRR470 pKa = 11.84WSGILVRR477 pKa = 11.84NSQDD481 pKa = 2.91EE482 pKa = 4.13AAVPGACAAFFSAPQLSDD500 pKa = 3.01TTIAALTSSPLKK512 pKa = 9.19TLHH515 pKa = 7.05IGTTQCDD522 pKa = 3.94ADD524 pKa = 4.2TLASTLLNTRR534 pKa = 11.84KK535 pKa = 9.97SSPLANSLTDD545 pKa = 4.18LDD547 pKa = 4.1VTYY550 pKa = 10.74LATDD554 pKa = 4.27ALCEE558 pKa = 3.94ALGNCCVNLRR568 pKa = 11.84ILRR571 pKa = 11.84LSRR574 pKa = 11.84SRR576 pKa = 11.84YY577 pKa = 9.08FSVEE581 pKa = 3.59SLLAVLRR588 pKa = 11.84GCPRR592 pKa = 11.84LRR594 pKa = 11.84VLEE597 pKa = 4.6LEE599 pKa = 4.3SCEE602 pKa = 4.18QICDD606 pKa = 3.33EE607 pKa = 4.59SLIAVSKK614 pKa = 10.8APCCPHH620 pKa = 7.17LGTLILANDD629 pKa = 3.77WQLTDD634 pKa = 3.46TGIASLLRR642 pKa = 11.84PATSLVRR649 pKa = 11.84LDD651 pKa = 3.95VRR653 pKa = 11.84HH654 pKa = 6.2CPEE657 pKa = 3.61ISLPVLKK664 pKa = 10.62SLAAARR670 pKa = 11.84GHH672 pKa = 6.06ISEE675 pKa = 4.31ATRR678 pKa = 11.84DD679 pKa = 3.8GLTPRR684 pKa = 11.84HH685 pKa = 5.95PNVVAFLRR693 pKa = 11.84RR694 pKa = 11.84EE695 pKa = 3.84RR696 pKa = 11.84KK697 pKa = 9.15RR698 pKa = 11.84RR699 pKa = 11.84VAARR703 pKa = 11.84TITRR707 pKa = 11.84WLRR710 pKa = 11.84CKK712 pKa = 10.56LDD714 pKa = 3.32ARR716 pKa = 11.84VSAKK720 pKa = 9.34STLEE724 pKa = 3.37RR725 pKa = 11.84ALAHH729 pKa = 6.51FRR731 pKa = 11.84RR732 pKa = 11.84QKK734 pKa = 8.67VTAARR739 pKa = 11.84IQRR742 pKa = 11.84WYY744 pKa = 10.08RR745 pKa = 11.84CKK747 pKa = 10.51ADD749 pKa = 3.13QRR751 pKa = 11.84MQRR754 pKa = 11.84RR755 pKa = 11.84LIAEE759 pKa = 4.01ARR761 pKa = 11.84RR762 pKa = 11.84LRR764 pKa = 11.84VEE766 pKa = 3.53QCEE769 pKa = 4.47KK770 pKa = 8.37YY771 pKa = 9.54WRR773 pKa = 11.84WIQALCVLSRR783 pKa = 11.84RR784 pKa = 11.84LRR786 pKa = 11.84IFIHH790 pKa = 5.47MFIAARR796 pKa = 11.84QRR798 pKa = 11.84AVFEE802 pKa = 4.13KK803 pKa = 10.8AEE805 pKa = 4.08QLRR808 pKa = 11.84MLRR811 pKa = 11.84DD812 pKa = 3.29KK813 pKa = 11.06AATEE817 pKa = 3.75IQRR820 pKa = 11.84IARR823 pKa = 11.84GWLGRR828 pKa = 11.84RR829 pKa = 11.84RR830 pKa = 11.84AVDD833 pKa = 3.02ARR835 pKa = 11.84HH836 pKa = 6.78ARR838 pKa = 11.84EE839 pKa = 3.74LWNKK843 pKa = 9.22RR844 pKa = 11.84RR845 pKa = 11.84AEE847 pKa = 3.99AATDD851 pKa = 3.39VQRR854 pKa = 11.84VFRR857 pKa = 11.84GHH859 pKa = 5.45QSRR862 pKa = 11.84KK863 pKa = 8.14GTAQLRR869 pKa = 11.84RR870 pKa = 11.84EE871 pKa = 4.14YY872 pKa = 10.75EE873 pKa = 3.87AVIQKK878 pKa = 10.26AIQLHH883 pKa = 4.84GTIFLAGLHH892 pKa = 5.3IQRR895 pKa = 11.84LIRR898 pKa = 11.84GFLGRR903 pKa = 11.84QYY905 pKa = 11.74AKK907 pKa = 9.63RR908 pKa = 11.84CAVAAYY914 pKa = 8.84EE915 pKa = 4.12LLQSKK920 pKa = 9.76IARR923 pKa = 11.84AKK925 pKa = 9.9QIQRR929 pKa = 11.84AYY931 pKa = 8.55RR932 pKa = 11.84AHH934 pKa = 7.02IARR937 pKa = 11.84VSLRR941 pKa = 11.84RR942 pKa = 11.84ILFRR946 pKa = 11.84GATALQKK953 pKa = 10.28VFRR956 pKa = 11.84GFRR959 pKa = 11.84GRR961 pKa = 11.84RR962 pKa = 11.84DD963 pKa = 3.0ARR965 pKa = 11.84VIVLGRR971 pKa = 11.84GYY973 pKa = 10.83ASHH976 pKa = 7.07PRR978 pKa = 11.84ILILMKK984 pKa = 10.39YY985 pKa = 10.1SIYY988 pKa = 9.92TQDD991 pKa = 3.43IEE993 pKa = 5.86DD994 pKa = 3.92FVDD997 pKa = 4.13DD998 pKa = 4.44VRR1000 pKa = 11.84HH1001 pKa = 5.82AYY1003 pKa = 9.6AWLVSDD1009 pKa = 5.49SAGIWKK1015 pKa = 8.67TLEE1018 pKa = 4.13HH1019 pKa = 6.42EE1020 pKa = 4.32RR1021 pKa = 11.84SSISSAVSLRR1031 pKa = 11.84LARR1034 pKa = 11.84FEE1036 pKa = 5.17ALLCLRR1042 pKa = 11.84RR1043 pKa = 11.84RR1044 pKa = 11.84SATKK1048 pKa = 9.96IQATWRR1054 pKa = 11.84MWLSKK1059 pKa = 10.82ALATARR1065 pKa = 11.84RR1066 pKa = 11.84LRR1068 pKa = 11.84NDD1070 pKa = 2.92RR1071 pKa = 11.84HH1072 pKa = 5.56RR1073 pKa = 11.84QHH1075 pKa = 6.27TAILDD1080 pKa = 4.79AIASPQQDD1088 pKa = 2.48RR1089 pKa = 11.84RR1090 pKa = 11.84LFLHH1094 pKa = 6.75RR1095 pKa = 11.84GAVHH1099 pKa = 6.8SIVSLYY1105 pKa = 10.24RR1106 pKa = 11.84ASLTTRR1112 pKa = 11.84GWLTPTYY1119 pKa = 10.24IKK1121 pKa = 10.49LLHH1124 pKa = 6.71RR1125 pKa = 11.84SATQIQALVRR1135 pKa = 11.84GHH1137 pKa = 6.81FGRR1140 pKa = 11.84VYY1142 pKa = 9.21TKK1144 pKa = 8.89WFRR1147 pKa = 11.84YY1148 pKa = 8.94EE1149 pKa = 3.67LTRR1152 pKa = 11.84AAAIVQRR1159 pKa = 11.84VWRR1162 pKa = 11.84GKK1164 pKa = 9.26LGKK1167 pKa = 9.75MMWRR1171 pKa = 11.84SLMEE1175 pKa = 3.61EE1176 pKa = 3.99RR1177 pKa = 11.84QQLRR1181 pKa = 11.84RR1182 pKa = 11.84VQEE1185 pKa = 4.13EE1186 pKa = 4.04GDD1188 pKa = 3.53RR1189 pKa = 11.84AALIAQKK1196 pKa = 8.67QTAHH1200 pKa = 6.68HH1201 pKa = 6.25ALEE1204 pKa = 4.23AFEE1207 pKa = 6.0RR1208 pKa = 11.84EE1209 pKa = 4.26TQHH1212 pKa = 6.78VVVLQRR1218 pKa = 11.84WYY1220 pKa = 8.97RR1221 pKa = 11.84TLKK1224 pKa = 9.02NRR1226 pKa = 11.84QVFRR1230 pKa = 11.84EE1231 pKa = 4.2ARR1233 pKa = 11.84DD1234 pKa = 3.47HH1235 pKa = 7.23RR1236 pKa = 11.84DD1237 pKa = 3.29RR1238 pKa = 11.84EE1239 pKa = 4.11AHH1241 pKa = 5.37TRR1243 pKa = 11.84AEE1245 pKa = 4.12NKK1247 pKa = 9.78VDD1249 pKa = 3.46GVLRR1253 pKa = 11.84NATGSVVFQARR1264 pKa = 11.84VWRR1267 pKa = 11.84DD1268 pKa = 3.1CVDD1271 pKa = 3.24RR1272 pKa = 11.84KK1273 pKa = 9.16TEE1275 pKa = 3.97LVALEE1280 pKa = 4.44EE1281 pKa = 4.53GQCVAMEE1288 pKa = 4.17KK1289 pKa = 10.37EE1290 pKa = 3.8IEE1292 pKa = 4.15EE1293 pKa = 4.14LKK1295 pKa = 10.45KK1296 pKa = 10.96ACIEE1300 pKa = 3.92AHH1302 pKa = 5.95TGSTQASRR1310 pKa = 11.84EE1311 pKa = 3.83FVEE1314 pKa = 4.12LSKK1317 pKa = 10.91RR1318 pKa = 11.84KK1319 pKa = 10.02NEE1321 pKa = 4.02FEE1323 pKa = 3.97RR1324 pKa = 11.84SRR1326 pKa = 11.84TRR1328 pKa = 11.84RR1329 pKa = 11.84KK1330 pKa = 9.96KK1331 pKa = 8.94ATEE1334 pKa = 3.69AVKK1337 pKa = 10.65QRR1339 pKa = 11.84IQPFAVRR1346 pKa = 11.84AKK1348 pKa = 9.85QLTMEE1353 pKa = 4.63SARR1356 pKa = 11.84EE1357 pKa = 3.85LNANRR1362 pKa = 11.84QLQVEE1367 pKa = 4.55LRR1369 pKa = 11.84RR1370 pKa = 11.84IRR1372 pKa = 11.84TEE1374 pKa = 3.38LRR1376 pKa = 11.84KK1377 pKa = 9.59FHH1379 pKa = 6.31VNLRR1383 pKa = 11.84GRR1385 pKa = 11.84LPMEE1389 pKa = 4.39PLLLSGDD1396 pKa = 3.89IEE1398 pKa = 4.46SLLAGLTGEE1407 pKa = 4.47GTSDD1411 pKa = 5.31DD1412 pKa = 4.37EE1413 pKa = 5.03DD1414 pKa = 4.02TTTTT1418 pKa = 3.6

Molecular weight:
159.8 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15887

0

15887

8238870

36

8814

518.6

57.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.663 ± 0.021

1.595 ± 0.008

5.716 ± 0.012

6.668 ± 0.022

3.778 ± 0.014

5.949 ± 0.019

2.287 ± 0.008

4.303 ± 0.014

5.24 ± 0.019

9.355 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.442 ± 0.008

3.73 ± 0.01

4.624 ± 0.016

4.208 ± 0.015

6.069 ± 0.019

8.288 ± 0.024

6.043 ± 0.016

7.058 ± 0.014

1.204 ± 0.007

2.666 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski