Phytophthora nicotianae (Buckeye rot agent)
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15887 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W8C4X9|A0A0W8C4X9_PHYNI Uncharacterized protein OS=Phytophthora nicotianae OX=4790 GN=AM587_10000106 PE=4 SV=1
MM1 pKa = 7.65 LSRR4 pKa = 11.84 YY5 pKa = 8.89 AVQLALIAASIAVFSSSSDD24 pKa = 3.06 ACLTGLFTGNDD35 pKa = 3.94 CDD37 pKa = 4.77 SDD39 pKa = 3.98 STSISLLGGTVDD51 pKa = 4.5 APCDD55 pKa = 3.64 EE56 pKa = 4.87 DD57 pKa = 4.08 TPSILSGGLLGGGLTGGLSGGITADD82 pKa = 4.07 APCDD86 pKa = 3.95 DD87 pKa = 4.9 DD88 pKa = 3.92 TPSIVSSGLTSGLTSGLSSTGLSGVTADD116 pKa = 4.67 IPCDD120 pKa = 3.59 DD121 pKa = 4.29 TPSVISSGLPSTGASTGVSTSTGTQQASATNSGTSATASIPSALTSSSPSTATGASTTASGTATSTATATSTAQGTMTSSAVDD204 pKa = 3.67 PSTAAQTSTAADD216 pKa = 3.92 PATKK220 pKa = 10.19 AGVSSAVSTDD230 pKa = 3.2 PVTKK234 pKa = 10.3 SGTSASTTLNSAAQHH249 pKa = 5.76 LRR251 pKa = 11.84 HH252 pKa = 6.1
Molecular weight: 23.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.592
IPC_protein 3.605
Toseland 3.363
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.656
Rodwell 3.439
Grimsley 3.287
Solomon 3.617
Lehninger 3.579
Nozaki 3.77
DTASelect 4.113
Thurlkill 3.465
EMBOSS 3.656
Sillero 3.745
Patrickios 0.846
IPC_peptide 3.605
IPC2_peptide 3.706
IPC2.peptide.svr19 3.686
Protein with the highest isoelectric point:
>tr|A0A0W8CN50|A0A0W8CN50_PHYNI HEAT repeat-containing protein 5B OS=Phytophthora nicotianae OX=4790 GN=AM587_10010541 PE=4 SV=1
MM1 pKa = 7.38 VNDD4 pKa = 4.39 GARR7 pKa = 11.84 RR8 pKa = 11.84 AKK10 pKa = 9.98 KK11 pKa = 10.18 RR12 pKa = 11.84 PLPLPLAPVVPIAARR27 pKa = 11.84 VSTNHH32 pKa = 6.3 KK33 pKa = 10.45 DD34 pKa = 3.54 AVRR37 pKa = 11.84 TNASSNEE44 pKa = 3.85 YY45 pKa = 9.88 RR46 pKa = 11.84 SSSAKK51 pKa = 9.44 AHH53 pKa = 5.67 EE54 pKa = 4.59 RR55 pKa = 11.84 NLAKK59 pKa = 10.51 LQLHH63 pKa = 6.68 LPRR66 pKa = 11.84 DD67 pKa = 3.87 VVFFDD72 pKa = 3.19 PRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 VYY78 pKa = 10.39 HH79 pKa = 5.0 SHH81 pKa = 6.36 VDD83 pKa = 3.5 LSGWRR88 pKa = 11.84 VTDD91 pKa = 4.22 ASLQQLQLNNDD102 pKa = 3.46 RR103 pKa = 11.84 DD104 pKa = 4.3 DD105 pKa = 4.62 NPVKK109 pKa = 10.74 SSFHH113 pKa = 6.15 TLTVDD118 pKa = 3.8 GSAHH122 pKa = 5.61 ISARR126 pKa = 11.84 GLLLMLEE133 pKa = 4.41 VGTAGGGMFRR143 pKa = 11.84 ALRR146 pKa = 11.84 LPHH149 pKa = 6.39 CQLLSRR155 pKa = 11.84 GGGEE159 pKa = 3.89 ILDD162 pKa = 4.35 GGSLPRR168 pKa = 11.84 SLTEE172 pKa = 4.48 LDD174 pKa = 3.57 VSSCEE179 pKa = 4.04 WVDD182 pKa = 4.17 DD183 pKa = 3.94 KK184 pKa = 11.32 FLRR187 pKa = 11.84 TLARR191 pKa = 11.84 HH192 pKa = 5.97 CSVLAQVTLAHH203 pKa = 6.58 CRR205 pKa = 11.84 RR206 pKa = 11.84 VTDD209 pKa = 3.36 YY210 pKa = 11.2 GVAAFGEE217 pKa = 5.03 SYY219 pKa = 10.66 AASLTSLDD227 pKa = 3.59 VSFCTKK233 pKa = 9.82 LTDD236 pKa = 3.52 TALLALLVGSSSSPAVPGGAQMATLSASARR266 pKa = 11.84 IRR268 pKa = 11.84 VLNVAGLPLVDD279 pKa = 4.58 GLTLLGLRR287 pKa = 11.84 GPCASRR293 pKa = 11.84 LEE295 pKa = 4.1 VLNMSGCTVLRR306 pKa = 11.84 VAALQRR312 pKa = 11.84 LAQVRR317 pKa = 11.84 ALIRR321 pKa = 11.84 LTKK324 pKa = 10.26 LDD326 pKa = 4.09 LSRR329 pKa = 11.84 CSLVDD334 pKa = 3.81 DD335 pKa = 5.32 QILTALGLACPKK347 pKa = 10.51 LAMLLLAFCSNITDD361 pKa = 3.33 VGIRR365 pKa = 11.84 RR366 pKa = 11.84 LVGEE370 pKa = 4.17 TAIVRR375 pKa = 11.84 ADD377 pKa = 3.5 QNDD380 pKa = 4.48 GAGHH384 pKa = 6.62 EE385 pKa = 4.26 PTIEE389 pKa = 4.12 DD390 pKa = 3.45 QEE392 pKa = 4.72 DD393 pKa = 3.58 FGLEE397 pKa = 3.94 NTSRR401 pKa = 11.84 AIPRR405 pKa = 11.84 NGEE408 pKa = 3.85 SGCRR412 pKa = 11.84 HH413 pKa = 6.89 LEE415 pKa = 3.93 TLDD418 pKa = 3.13 ITGCFQVTTRR428 pKa = 11.84 GISALGIRR436 pKa = 11.84 CPQLRR441 pKa = 11.84 SVTLDD446 pKa = 3.02 GVRR449 pKa = 11.84 RR450 pKa = 11.84 LDD452 pKa = 3.39 SSGIRR457 pKa = 11.84 ALLHH461 pKa = 5.66 GCRR464 pKa = 11.84 KK465 pKa = 9.61 LRR467 pKa = 11.84 TIRR470 pKa = 11.84 WSGILVRR477 pKa = 11.84 NSQDD481 pKa = 2.91 EE482 pKa = 4.13 AAVPGACAAFFSAPQLSDD500 pKa = 3.01 TTIAALTSSPLKK512 pKa = 9.19 TLHH515 pKa = 7.05 IGTTQCDD522 pKa = 3.94 ADD524 pKa = 4.2 TLASTLLNTRR534 pKa = 11.84 KK535 pKa = 9.97 SSPLANSLTDD545 pKa = 4.18 LDD547 pKa = 4.1 VTYY550 pKa = 10.74 LATDD554 pKa = 4.27 ALCEE558 pKa = 3.94 ALGNCCVNLRR568 pKa = 11.84 ILRR571 pKa = 11.84 LSRR574 pKa = 11.84 SRR576 pKa = 11.84 YY577 pKa = 9.08 FSVEE581 pKa = 3.59 SLLAVLRR588 pKa = 11.84 GCPRR592 pKa = 11.84 LRR594 pKa = 11.84 VLEE597 pKa = 4.6 LEE599 pKa = 4.3 SCEE602 pKa = 4.18 QICDD606 pKa = 3.33 EE607 pKa = 4.59 SLIAVSKK614 pKa = 10.8 APCCPHH620 pKa = 7.17 LGTLILANDD629 pKa = 3.77 WQLTDD634 pKa = 3.46 TGIASLLRR642 pKa = 11.84 PATSLVRR649 pKa = 11.84 LDD651 pKa = 3.95 VRR653 pKa = 11.84 HH654 pKa = 6.2 CPEE657 pKa = 3.61 ISLPVLKK664 pKa = 10.62 SLAAARR670 pKa = 11.84 GHH672 pKa = 6.06 ISEE675 pKa = 4.31 ATRR678 pKa = 11.84 DD679 pKa = 3.8 GLTPRR684 pKa = 11.84 HH685 pKa = 5.95 PNVVAFLRR693 pKa = 11.84 RR694 pKa = 11.84 EE695 pKa = 3.84 RR696 pKa = 11.84 KK697 pKa = 9.15 RR698 pKa = 11.84 RR699 pKa = 11.84 VAARR703 pKa = 11.84 TITRR707 pKa = 11.84 WLRR710 pKa = 11.84 CKK712 pKa = 10.56 LDD714 pKa = 3.32 ARR716 pKa = 11.84 VSAKK720 pKa = 9.34 STLEE724 pKa = 3.37 RR725 pKa = 11.84 ALAHH729 pKa = 6.51 FRR731 pKa = 11.84 RR732 pKa = 11.84 QKK734 pKa = 8.67 VTAARR739 pKa = 11.84 IQRR742 pKa = 11.84 WYY744 pKa = 10.08 RR745 pKa = 11.84 CKK747 pKa = 10.51 ADD749 pKa = 3.13 QRR751 pKa = 11.84 MQRR754 pKa = 11.84 RR755 pKa = 11.84 LIAEE759 pKa = 4.01 ARR761 pKa = 11.84 RR762 pKa = 11.84 LRR764 pKa = 11.84 VEE766 pKa = 3.53 QCEE769 pKa = 4.47 KK770 pKa = 8.37 YY771 pKa = 9.54 WRR773 pKa = 11.84 WIQALCVLSRR783 pKa = 11.84 RR784 pKa = 11.84 LRR786 pKa = 11.84 IFIHH790 pKa = 5.47 MFIAARR796 pKa = 11.84 QRR798 pKa = 11.84 AVFEE802 pKa = 4.13 KK803 pKa = 10.8 AEE805 pKa = 4.08 QLRR808 pKa = 11.84 MLRR811 pKa = 11.84 DD812 pKa = 3.29 KK813 pKa = 11.06 AATEE817 pKa = 3.75 IQRR820 pKa = 11.84 IARR823 pKa = 11.84 GWLGRR828 pKa = 11.84 RR829 pKa = 11.84 RR830 pKa = 11.84 AVDD833 pKa = 3.02 ARR835 pKa = 11.84 HH836 pKa = 6.78 ARR838 pKa = 11.84 EE839 pKa = 3.74 LWNKK843 pKa = 9.22 RR844 pKa = 11.84 RR845 pKa = 11.84 AEE847 pKa = 3.99 AATDD851 pKa = 3.39 VQRR854 pKa = 11.84 VFRR857 pKa = 11.84 GHH859 pKa = 5.45 QSRR862 pKa = 11.84 KK863 pKa = 8.14 GTAQLRR869 pKa = 11.84 RR870 pKa = 11.84 EE871 pKa = 4.14 YY872 pKa = 10.75 EE873 pKa = 3.87 AVIQKK878 pKa = 10.26 AIQLHH883 pKa = 4.84 GTIFLAGLHH892 pKa = 5.3 IQRR895 pKa = 11.84 LIRR898 pKa = 11.84 GFLGRR903 pKa = 11.84 QYY905 pKa = 11.74 AKK907 pKa = 9.63 RR908 pKa = 11.84 CAVAAYY914 pKa = 8.84 EE915 pKa = 4.12 LLQSKK920 pKa = 9.76 IARR923 pKa = 11.84 AKK925 pKa = 9.9 QIQRR929 pKa = 11.84 AYY931 pKa = 8.55 RR932 pKa = 11.84 AHH934 pKa = 7.02 IARR937 pKa = 11.84 VSLRR941 pKa = 11.84 RR942 pKa = 11.84 ILFRR946 pKa = 11.84 GATALQKK953 pKa = 10.28 VFRR956 pKa = 11.84 GFRR959 pKa = 11.84 GRR961 pKa = 11.84 RR962 pKa = 11.84 DD963 pKa = 3.0 ARR965 pKa = 11.84 VIVLGRR971 pKa = 11.84 GYY973 pKa = 10.83 ASHH976 pKa = 7.07 PRR978 pKa = 11.84 ILILMKK984 pKa = 10.39 YY985 pKa = 10.1 SIYY988 pKa = 9.92 TQDD991 pKa = 3.43 IEE993 pKa = 5.86 DD994 pKa = 3.92 FVDD997 pKa = 4.13 DD998 pKa = 4.44 VRR1000 pKa = 11.84 HH1001 pKa = 5.82 AYY1003 pKa = 9.6 AWLVSDD1009 pKa = 5.49 SAGIWKK1015 pKa = 8.67 TLEE1018 pKa = 4.13 HH1019 pKa = 6.42 EE1020 pKa = 4.32 RR1021 pKa = 11.84 SSISSAVSLRR1031 pKa = 11.84 LARR1034 pKa = 11.84 FEE1036 pKa = 5.17 ALLCLRR1042 pKa = 11.84 RR1043 pKa = 11.84 RR1044 pKa = 11.84 SATKK1048 pKa = 9.96 IQATWRR1054 pKa = 11.84 MWLSKK1059 pKa = 10.82 ALATARR1065 pKa = 11.84 RR1066 pKa = 11.84 LRR1068 pKa = 11.84 NDD1070 pKa = 2.92 RR1071 pKa = 11.84 HH1072 pKa = 5.56 RR1073 pKa = 11.84 QHH1075 pKa = 6.27 TAILDD1080 pKa = 4.79 AIASPQQDD1088 pKa = 2.48 RR1089 pKa = 11.84 RR1090 pKa = 11.84 LFLHH1094 pKa = 6.75 RR1095 pKa = 11.84 GAVHH1099 pKa = 6.8 SIVSLYY1105 pKa = 10.24 RR1106 pKa = 11.84 ASLTTRR1112 pKa = 11.84 GWLTPTYY1119 pKa = 10.24 IKK1121 pKa = 10.49 LLHH1124 pKa = 6.71 RR1125 pKa = 11.84 SATQIQALVRR1135 pKa = 11.84 GHH1137 pKa = 6.81 FGRR1140 pKa = 11.84 VYY1142 pKa = 9.21 TKK1144 pKa = 8.89 WFRR1147 pKa = 11.84 YY1148 pKa = 8.94 EE1149 pKa = 3.67 LTRR1152 pKa = 11.84 AAAIVQRR1159 pKa = 11.84 VWRR1162 pKa = 11.84 GKK1164 pKa = 9.26 LGKK1167 pKa = 9.75 MMWRR1171 pKa = 11.84 SLMEE1175 pKa = 3.61 EE1176 pKa = 3.99 RR1177 pKa = 11.84 QQLRR1181 pKa = 11.84 RR1182 pKa = 11.84 VQEE1185 pKa = 4.13 EE1186 pKa = 4.04 GDD1188 pKa = 3.53 RR1189 pKa = 11.84 AALIAQKK1196 pKa = 8.67 QTAHH1200 pKa = 6.68 HH1201 pKa = 6.25 ALEE1204 pKa = 4.23 AFEE1207 pKa = 6.0 RR1208 pKa = 11.84 EE1209 pKa = 4.26 TQHH1212 pKa = 6.78 VVVLQRR1218 pKa = 11.84 WYY1220 pKa = 8.97 RR1221 pKa = 11.84 TLKK1224 pKa = 9.02 NRR1226 pKa = 11.84 QVFRR1230 pKa = 11.84 EE1231 pKa = 4.2 ARR1233 pKa = 11.84 DD1234 pKa = 3.47 HH1235 pKa = 7.23 RR1236 pKa = 11.84 DD1237 pKa = 3.29 RR1238 pKa = 11.84 EE1239 pKa = 4.11 AHH1241 pKa = 5.37 TRR1243 pKa = 11.84 AEE1245 pKa = 4.12 NKK1247 pKa = 9.78 VDD1249 pKa = 3.46 GVLRR1253 pKa = 11.84 NATGSVVFQARR1264 pKa = 11.84 VWRR1267 pKa = 11.84 DD1268 pKa = 3.1 CVDD1271 pKa = 3.24 RR1272 pKa = 11.84 KK1273 pKa = 9.16 TEE1275 pKa = 3.97 LVALEE1280 pKa = 4.44 EE1281 pKa = 4.53 GQCVAMEE1288 pKa = 4.17 KK1289 pKa = 10.37 EE1290 pKa = 3.8 IEE1292 pKa = 4.15 EE1293 pKa = 4.14 LKK1295 pKa = 10.45 KK1296 pKa = 10.96 ACIEE1300 pKa = 3.92 AHH1302 pKa = 5.95 TGSTQASRR1310 pKa = 11.84 EE1311 pKa = 3.83 FVEE1314 pKa = 4.12 LSKK1317 pKa = 10.91 RR1318 pKa = 11.84 KK1319 pKa = 10.02 NEE1321 pKa = 4.02 FEE1323 pKa = 3.97 RR1324 pKa = 11.84 SRR1326 pKa = 11.84 TRR1328 pKa = 11.84 RR1329 pKa = 11.84 KK1330 pKa = 9.96 KK1331 pKa = 8.94 ATEE1334 pKa = 3.69 AVKK1337 pKa = 10.65 QRR1339 pKa = 11.84 IQPFAVRR1346 pKa = 11.84 AKK1348 pKa = 9.85 QLTMEE1353 pKa = 4.63 SARR1356 pKa = 11.84 EE1357 pKa = 3.85 LNANRR1362 pKa = 11.84 QLQVEE1367 pKa = 4.55 LRR1369 pKa = 11.84 RR1370 pKa = 11.84 IRR1372 pKa = 11.84 TEE1374 pKa = 3.38 LRR1376 pKa = 11.84 KK1377 pKa = 9.59 FHH1379 pKa = 6.31 VNLRR1383 pKa = 11.84 GRR1385 pKa = 11.84 LPMEE1389 pKa = 4.39 PLLLSGDD1396 pKa = 3.89 IEE1398 pKa = 4.46 SLLAGLTGEE1407 pKa = 4.47 GTSDD1411 pKa = 5.31 DD1412 pKa = 4.37 EE1413 pKa = 5.03 DD1414 pKa = 4.02 TTTTT1418 pKa = 3.6
Molecular weight: 159.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 9.487
IPC_protein 10.35
Toseland 10.672
ProMoST 10.423
Dawson 10.745
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.847
Grimsley 10.789
Solomon 10.906
Lehninger 10.877
Nozaki 10.687
DTASelect 10.482
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.701
Patrickios 10.511
IPC_peptide 10.921
IPC2_peptide 9.78
IPC2.peptide.svr19 8.671
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15887
0
15887
8238870
36
8814
518.6
57.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.663 ± 0.021
1.595 ± 0.008
5.716 ± 0.012
6.668 ± 0.022
3.778 ± 0.014
5.949 ± 0.019
2.287 ± 0.008
4.303 ± 0.014
5.24 ± 0.019
9.355 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.442 ± 0.008
3.73 ± 0.01
4.624 ± 0.016
4.208 ± 0.015
6.069 ± 0.019
8.288 ± 0.024
6.043 ± 0.016
7.058 ± 0.014
1.204 ± 0.007
2.666 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here