Avon-Heathcote Estuary associated circular virus 13

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IB23|A0A0C5IB23_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 13 OX=1618236 PE=3 SV=1
MM1 pKa = 7.52TCRR4 pKa = 11.84NWCLTLNNFTPEE16 pKa = 3.87EE17 pKa = 4.12EE18 pKa = 4.21EE19 pKa = 4.09VLKK22 pKa = 10.91EE23 pKa = 3.84EE24 pKa = 4.32KK25 pKa = 9.91PWIKK29 pKa = 10.1FVVYY33 pKa = 9.22QVEE36 pKa = 4.43MGEE39 pKa = 4.12SGTPHH44 pKa = 5.61LQGYY48 pKa = 9.5IEE50 pKa = 4.51TNGGVRR56 pKa = 11.84MSKK59 pKa = 8.9MKK61 pKa = 10.64RR62 pKa = 11.84MLEE65 pKa = 4.02RR66 pKa = 11.84GHH68 pKa = 6.17WEE70 pKa = 3.45QRR72 pKa = 11.84LGSRR76 pKa = 11.84AQALTYY82 pKa = 10.08CLKK85 pKa = 10.09EE86 pKa = 4.2DD87 pKa = 3.48SRR89 pKa = 11.84VRR91 pKa = 11.84GPYY94 pKa = 10.41LIGLSQEE101 pKa = 4.2EE102 pKa = 4.38LNSYY106 pKa = 9.51LKK108 pKa = 10.67KK109 pKa = 10.56NPKK112 pKa = 8.85STDD115 pKa = 3.22LNVIKK120 pKa = 10.69DD121 pKa = 3.87KK122 pKa = 11.04IKK124 pKa = 10.98AGASEE129 pKa = 5.29LDD131 pKa = 3.39VADD134 pKa = 3.54AHH136 pKa = 6.21FGSWVRR142 pKa = 11.84YY143 pKa = 7.95YY144 pKa = 10.98KK145 pKa = 10.61AFEE148 pKa = 4.31RR149 pKa = 11.84YY150 pKa = 9.04RR151 pKa = 11.84CLNTKK156 pKa = 9.61KK157 pKa = 10.38RR158 pKa = 11.84STIDD162 pKa = 3.0KK163 pKa = 10.09VIIIVGPTGTGKK175 pKa = 10.38SRR177 pKa = 11.84FCMEE181 pKa = 4.71QYY183 pKa = 10.85PDD185 pKa = 4.6AYY187 pKa = 9.38WKK189 pKa = 10.07QRR191 pKa = 11.84SNWWCGYY198 pKa = 9.49SGEE201 pKa = 4.29STIIIDD207 pKa = 4.7EE208 pKa = 4.71FYY210 pKa = 11.05GWIPFDD216 pKa = 3.5MLLRR220 pKa = 11.84LCDD223 pKa = 4.2RR224 pKa = 11.84YY225 pKa = 10.39PLLLEE230 pKa = 4.37TKK232 pKa = 10.18GGQCQCVATTIVITSNHH249 pKa = 6.39HH250 pKa = 5.7PNTWYY255 pKa = 10.41KK256 pKa = 10.17NSYY259 pKa = 9.69VEE261 pKa = 3.78SLYY264 pKa = 10.91RR265 pKa = 11.84RR266 pKa = 11.84FTNIIYY272 pKa = 9.25MPALGVSEE280 pKa = 4.57HH281 pKa = 6.61LNSIEE286 pKa = 4.68DD287 pKa = 3.32LHH289 pKa = 8.32LNYY292 pKa = 10.26FNFPPNN298 pKa = 3.17

Molecular weight: 34.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IB23|A0A0C5IB23_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 13 OX=1618236 PE=3 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84LLKK5 pKa = 10.37PKK7 pKa = 9.92EE8 pKa = 3.75YY9 pKa = 10.5SQGKK13 pKa = 9.75RR14 pKa = 11.84IFGQAFATAGAAAMRR29 pKa = 11.84ARR31 pKa = 11.84KK32 pKa = 9.46RR33 pKa = 11.84SRR35 pKa = 11.84GYY37 pKa = 10.69SRR39 pKa = 11.84TNTGRR44 pKa = 11.84RR45 pKa = 11.84TGMRR49 pKa = 11.84SKK51 pKa = 10.79KK52 pKa = 10.2RR53 pKa = 11.84IGLGRR58 pKa = 11.84TLTTRR63 pKa = 11.84QKK65 pKa = 10.9DD66 pKa = 3.76FSRR69 pKa = 11.84TYY71 pKa = 9.68VRR73 pKa = 11.84KK74 pKa = 9.65PMPYY78 pKa = 8.13RR79 pKa = 11.84KK80 pKa = 9.31KK81 pKa = 10.64RR82 pKa = 11.84KK83 pKa = 8.2WVQFKK88 pKa = 11.28NKK90 pKa = 9.29VNTVAEE96 pKa = 4.72DD97 pKa = 3.63EE98 pKa = 5.29LGTQTVIFNNQDD110 pKa = 2.97TAGITEE116 pKa = 4.73GVAGGQGTLEE126 pKa = 3.92ASLYY130 pKa = 10.13GVAGRR135 pKa = 11.84PDD137 pKa = 3.39RR138 pKa = 11.84HH139 pKa = 6.34NDD141 pKa = 3.19LAIVRR146 pKa = 11.84GMLNDD151 pKa = 4.17GDD153 pKa = 3.94PTAAAGINVDD163 pKa = 4.2DD164 pKa = 3.63TTKK167 pKa = 10.89YY168 pKa = 9.71IFHH171 pKa = 6.9SGVLDD176 pKa = 3.09ITLRR180 pKa = 11.84NTSKK184 pKa = 10.37QRR186 pKa = 11.84EE187 pKa = 4.37TANDD191 pKa = 4.11PYY193 pKa = 10.84IPWNEE198 pKa = 3.97CSLEE202 pKa = 3.82VDD204 pKa = 4.55LYY206 pKa = 10.76IISSNKK212 pKa = 8.68KK213 pKa = 7.16WQPDD217 pKa = 3.58TLSIRR222 pKa = 11.84QCLDD226 pKa = 2.9SGFATTKK233 pKa = 10.83GLDD236 pKa = 3.43GAGNQLTRR244 pKa = 11.84SKK246 pKa = 10.84RR247 pKa = 11.84GVTVFDD253 pKa = 4.52CPDD256 pKa = 4.51GISKK260 pKa = 10.9YY261 pKa = 10.6GLQVQKK267 pKa = 9.33KK268 pKa = 6.23TKK270 pKa = 10.0YY271 pKa = 9.8FIPYY275 pKa = 8.01GQQVTYY281 pKa = 9.71QMRR284 pKa = 11.84DD285 pKa = 3.44PKK287 pKa = 10.17RR288 pKa = 11.84HH289 pKa = 3.83VMRR292 pKa = 11.84RR293 pKa = 11.84GNMSTGNYY301 pKa = 9.6GNTPNKK307 pKa = 9.83PGLTHH312 pKa = 6.32YY313 pKa = 10.8VFISYY318 pKa = 10.33KK319 pKa = 10.21PVTGLPNLADD329 pKa = 3.63INNIPQLSMGNTRR342 pKa = 11.84KK343 pKa = 10.32YY344 pKa = 7.99MFKK347 pKa = 10.68VEE349 pKa = 4.18GMNDD353 pKa = 2.82TRR355 pKa = 11.84DD356 pKa = 3.66EE357 pKa = 4.35YY358 pKa = 11.44KK359 pKa = 10.64SGSIVDD365 pKa = 4.17FNN367 pKa = 4.11

Molecular weight: 41.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

665

298

367

332.5

38.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.511 ± 0.759

1.805 ± 0.798

4.962 ± 0.834

4.962 ± 1.809

3.308 ± 0.031

8.421 ± 1.122

1.654 ± 0.456

5.414 ± 0.411

7.368 ± 0.431

7.218 ± 0.989

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.113

6.015 ± 0.204

4.361 ± 0.001

3.91 ± 0.584

7.368 ± 0.872

5.865 ± 0.336

7.669 ± 1.069

5.263 ± 0.151

1.805 ± 0.798

5.263 ± 0.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski