Soil-borne cereal mosaic virus
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9QCE7|Q9QCE7_9VIRU Putative movement protein OS=Soil-borne cereal mosaic virus OX=100887 GN=ORF3 PE=4 SV=1
MM1 pKa = 8.27 DD2 pKa = 5.47 KK3 pKa = 10.11 LTRR6 pKa = 11.84 LKK8 pKa = 11.35 GEE10 pKa = 4.17 VDD12 pKa = 3.63 RR13 pKa = 11.84 SLQKK17 pKa = 10.96 AADD20 pKa = 3.68 KK21 pKa = 9.92 VTAEE25 pKa = 4.39 STEE28 pKa = 4.14 SLQSKK33 pKa = 8.96 VVVQLPQMAVKK44 pKa = 10.55 NGYY47 pKa = 7.5 TGYY50 pKa = 10.76 NKK52 pKa = 9.98 EE53 pKa = 4.34 LNLMASIHH61 pKa = 5.47 PFIRR65 pKa = 11.84 LGTLISQIEE74 pKa = 4.46 GWQATRR80 pKa = 11.84 ASILTHH86 pKa = 6.51 LGVMLNGVSKK96 pKa = 10.86 LGEE99 pKa = 3.82 RR100 pKa = 11.84 NFFSRR105 pKa = 11.84 QKK107 pKa = 10.75 RR108 pKa = 11.84 FGTHH112 pKa = 4.74 TQDD115 pKa = 3.63 GDD117 pKa = 4.7 EE118 pKa = 4.48 IFCDD122 pKa = 3.66 LGGEE126 pKa = 3.95 AVMQIITRR134 pKa = 11.84 LTVSLQSAKK143 pKa = 11.07 GEE145 pKa = 4.16 GSQTRR150 pKa = 11.84 NAMIGTTPTTNQFEE164 pKa = 4.61 GEE166 pKa = 4.22 EE167 pKa = 3.99 QGQTDD172 pKa = 3.5 QTLAISNALAEE183 pKa = 4.56 FMTFIHH189 pKa = 6.29 TKK191 pKa = 10.66 DD192 pKa = 3.26 FTTNEE197 pKa = 5.1 CYY199 pKa = 9.71 TQDD202 pKa = 3.26 SFEE205 pKa = 4.94 AKK207 pKa = 10.26 FNLKK211 pKa = 9.68 WEE213 pKa = 4.57 GTSS216 pKa = 3.25
Molecular weight: 23.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.697
IPC2_protein 5.664
IPC_protein 5.639
Toseland 5.944
ProMoST 5.893
Dawson 5.817
Bjellqvist 5.842
Wikipedia 5.779
Rodwell 5.779
Grimsley 6.071
Solomon 5.817
Lehninger 5.792
Nozaki 6.033
DTASelect 6.211
Thurlkill 6.173
EMBOSS 6.122
Sillero 6.135
Patrickios 4.558
IPC_peptide 5.83
IPC2_peptide 6.122
IPC2.peptide.svr19 6.197
Protein with the highest isoelectric point:
>tr|Q9QCE8|Q9QCE8_9VIRU Replicase OS=Soil-borne cereal mosaic virus OX=100887 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.51 GSKK4 pKa = 9.97 DD5 pKa = 3.5 VKK7 pKa = 10.59 GDD9 pKa = 3.58 SFEE12 pKa = 4.35 SVYY15 pKa = 11.47 NKK17 pKa = 10.12 ILDD20 pKa = 4.02 LQTEE24 pKa = 4.54 GAVGADD30 pKa = 3.11 SLKK33 pKa = 10.16 TNRR36 pKa = 11.84 QRR38 pKa = 11.84 SFNIEE43 pKa = 3.42 NKK45 pKa = 9.46 YY46 pKa = 9.71 VEE48 pKa = 4.41 KK49 pKa = 10.92 ALVQQDD55 pKa = 3.11 IATKK59 pKa = 9.51 MRR61 pKa = 11.84 EE62 pKa = 3.92 GWTTWTKK69 pKa = 8.77 TNKK72 pKa = 10.23 EE73 pKa = 3.79 EE74 pKa = 4.04 GTPYY78 pKa = 10.91 NMSYY82 pKa = 10.83 SCVLLNVIPTVPRR95 pKa = 11.84 GYY97 pKa = 10.36 SGTVEE102 pKa = 3.61 ISLIDD107 pKa = 3.63 SGLSPLEE114 pKa = 3.86 NVIPDD119 pKa = 3.58 QTQMMEE125 pKa = 4.71 LGNGPHH131 pKa = 5.95 VMCFFMHH138 pKa = 6.43 YY139 pKa = 10.1 GIPLNDD145 pKa = 3.69 EE146 pKa = 3.75 GRR148 pKa = 11.84 VIKK151 pKa = 10.72 LSFKK155 pKa = 10.5 IEE157 pKa = 3.67 AEE159 pKa = 4.09 MASKK163 pKa = 10.55 KK164 pKa = 9.47 MSVMNVYY171 pKa = 10.5 SYY173 pKa = 7.61 WTQKK177 pKa = 9.95 QGHH180 pKa = 6.52 LSVYY184 pKa = 9.15 TEE186 pKa = 4.3 PQRR189 pKa = 11.84 STCSKK194 pKa = 10.8 LLLGYY199 pKa = 10.47 DD200 pKa = 3.5 KK201 pKa = 10.98 SLKK204 pKa = 10.61 LKK206 pKa = 9.66 TRR208 pKa = 11.84 DD209 pKa = 3.25 DD210 pKa = 3.34 VRR212 pKa = 11.84 RR213 pKa = 11.84 FVPRR217 pKa = 11.84 SLATNNLEE225 pKa = 3.93 QSVPKK230 pKa = 9.86 MLPNSINILKK240 pKa = 10.53 EE241 pKa = 3.7 NVPLFKK247 pKa = 10.61 KK248 pKa = 10.67 EE249 pKa = 4.1 SVLDD253 pKa = 3.5 LTEE256 pKa = 4.21 QEE258 pKa = 4.26 RR259 pKa = 11.84 EE260 pKa = 4.09 KK261 pKa = 10.86 QKK263 pKa = 11.07 KK264 pKa = 10.14 LEE266 pKa = 4.07 TLRR269 pKa = 11.84 SVNEE273 pKa = 3.57 AQRR276 pKa = 11.84 RR277 pKa = 11.84 KK278 pKa = 9.71 SAQEE282 pKa = 3.55 MQKK285 pKa = 10.7 RR286 pKa = 11.84 NDD288 pKa = 3.52 DD289 pKa = 4.4 LIRR292 pKa = 11.84 ANVQKK297 pKa = 10.74 QKK299 pKa = 11.03 AKK301 pKa = 10.36 QSEE304 pKa = 3.79 IDD306 pKa = 3.63 EE307 pKa = 4.33 NRR309 pKa = 11.84 KK310 pKa = 8.43 NHH312 pKa = 6.09 YY313 pKa = 9.6 DD314 pKa = 3.29 VSGVKK319 pKa = 10.27 FGSVGSDD326 pKa = 2.64
Molecular weight: 37.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.437
IPC2_protein 8.346
IPC_protein 8.244
Toseland 9.019
ProMoST 8.785
Dawson 9.282
Bjellqvist 9.063
Wikipedia 9.428
Rodwell 9.56
Grimsley 9.311
Solomon 9.355
Lehninger 9.326
Nozaki 9.194
DTASelect 8.99
Thurlkill 9.165
EMBOSS 9.458
Sillero 9.282
Patrickios 4.914
IPC_peptide 9.355
IPC2_peptide 7.585
IPC2.peptide.svr19 7.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4625
165
1816
770.8
87.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.681 ± 0.475
1.751 ± 0.283
6.724 ± 0.552
7.157 ± 0.207
4.13 ± 0.349
5.514 ± 0.561
2.097 ± 0.173
4.822 ± 0.216
7.222 ± 0.687
8.951 ± 0.225
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.789 ± 0.17
4.411 ± 0.342
2.789 ± 0.225
3.308 ± 0.614
6.054 ± 0.343
6.876 ± 0.431
6.811 ± 0.465
7.286 ± 0.36
1.189 ± 0.086
3.395 ± 0.366
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here