Sweet potato leaf curl Henan virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4VLV7|S4VLV7_9GEMI Replication-associated protein OS=Sweet potato leaf curl Henan virus OX=1489311 PE=3 SV=1
MM1 pKa = 6.84VRR3 pKa = 11.84APGFRR8 pKa = 11.84LQAKK12 pKa = 9.92NIFLTYY18 pKa = 9.22PKK20 pKa = 10.38CPLSKK25 pKa = 10.35DD26 pKa = 3.47TVLEE30 pKa = 3.98LLKK33 pKa = 10.92GIQCPSDD40 pKa = 3.11KK41 pKa = 11.03LFIRR45 pKa = 11.84VAQEE49 pKa = 3.46KK50 pKa = 9.49HH51 pKa = 5.14QDD53 pKa = 3.46GSLHH57 pKa = 5.77LHH59 pKa = 6.73ALIQFKK65 pKa = 10.38GKK67 pKa = 9.96CQFTNPRR74 pKa = 11.84HH75 pKa = 6.27FDD77 pKa = 3.26LTHH80 pKa = 6.85PNSSTQYY87 pKa = 10.01HH88 pKa = 7.03PNFQSAKK95 pKa = 9.43SSSDD99 pKa = 2.98VKK101 pKa = 11.12AYY103 pKa = 9.77IEE105 pKa = 4.23KK106 pKa = 10.83DD107 pKa = 3.01QDD109 pKa = 3.85FVDD112 pKa = 3.69SGVFQVDD119 pKa = 3.07GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.4AYY134 pKa = 10.67AQALNTGCKK143 pKa = 9.76SEE145 pKa = 3.99ALQIIKK151 pKa = 9.12EE152 pKa = 4.21LCPKK156 pKa = 10.74DD157 pKa = 4.55FVLQFHH163 pKa = 7.32NINNNLDD170 pKa = 4.28RR171 pKa = 11.84IFSPPPSVYY180 pKa = 10.44SSPFSLSSFNNVPDD194 pKa = 5.62IISDD198 pKa = 3.84WAAEE202 pKa = 4.12NVMDD206 pKa = 5.53AAARR210 pKa = 11.84PDD212 pKa = 3.59RR213 pKa = 11.84PISIVIEE220 pKa = 4.52GPSRR224 pKa = 11.84IGKK227 pKa = 6.21TVWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.09VYY251 pKa = 10.85SNSAWYY257 pKa = 10.34NVIDD261 pKa = 4.95DD262 pKa = 4.8VNPQYY267 pKa = 11.15LKK269 pKa = 10.65HH270 pKa = 6.26FKK272 pKa = 10.69EE273 pKa = 4.43FMGAQKK279 pKa = 10.56DD280 pKa = 3.62WQSNCKK286 pKa = 9.38YY287 pKa = 10.21GKK289 pKa = 9.18PVQIKK294 pKa = 10.41GGIPTIFLCNPGEE307 pKa = 4.28GSSFKK312 pKa = 10.83LWLDD316 pKa = 3.34KK317 pKa = 11.07PEE319 pKa = 4.01QGALKK324 pKa = 10.11IWATANALFCDD335 pKa = 4.01VQSPFWVQEE344 pKa = 4.25EE345 pKa = 4.74VSHH348 pKa = 7.21SGATTHH354 pKa = 7.05RR355 pKa = 11.84GEE357 pKa = 4.33EE358 pKa = 4.34GQEE361 pKa = 3.89EE362 pKa = 4.85SSS364 pKa = 3.48

Molecular weight: 40.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4VLV7|S4VLV7_9GEMI Replication-associated protein OS=Sweet potato leaf curl Henan virus OX=1489311 PE=3 SV=1
MM1 pKa = 7.34VGRR4 pKa = 11.84IPVSRR9 pKa = 11.84RR10 pKa = 11.84FHH12 pKa = 6.81PYY14 pKa = 8.37GGRR17 pKa = 11.84PVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84LNFEE26 pKa = 3.93TAIVPYY32 pKa = 9.22TGNAVPIAARR42 pKa = 11.84SYY44 pKa = 9.09VPVSRR49 pKa = 11.84SVQMKK54 pKa = 9.24RR55 pKa = 11.84RR56 pKa = 11.84KK57 pKa = 9.73GDD59 pKa = 4.2RR60 pKa = 11.84IPKK63 pKa = 9.86GCVGPCKK70 pKa = 10.08VQDD73 pKa = 3.86YY74 pKa = 8.72EE75 pKa = 4.55FKK77 pKa = 10.32MDD79 pKa = 3.61VPHH82 pKa = 7.03SGTFVCVSDD91 pKa = 3.96FTRR94 pKa = 11.84GTGLTHH100 pKa = 7.28RR101 pKa = 11.84LGKK104 pKa = 9.86RR105 pKa = 11.84VCIKK109 pKa = 11.05SMGIDD114 pKa = 3.27GKK116 pKa = 11.25VWMDD120 pKa = 3.75DD121 pKa = 3.28NVAKK125 pKa = 10.24RR126 pKa = 11.84DD127 pKa = 3.48HH128 pKa = 6.56TNIITYY134 pKa = 9.02WLIRR138 pKa = 11.84DD139 pKa = 3.65RR140 pKa = 11.84RR141 pKa = 11.84PNKK144 pKa = 10.43DD145 pKa = 3.23PLTFAQAFTMYY156 pKa = 10.75DD157 pKa = 3.66NEE159 pKa = 4.15PTTAKK164 pKa = 10.17IRR166 pKa = 11.84MDD168 pKa = 3.48LRR170 pKa = 11.84DD171 pKa = 3.42RR172 pKa = 11.84MQVLKK177 pKa = 10.39KK178 pKa = 10.29FSVTVSGGPYY188 pKa = 8.51NHH190 pKa = 7.45KK191 pKa = 10.06EE192 pKa = 3.61QALVRR197 pKa = 11.84KK198 pKa = 8.79FFKK201 pKa = 10.63GLYY204 pKa = 8.96NHH206 pKa = 5.46VTYY209 pKa = 10.87NHH211 pKa = 6.65KK212 pKa = 10.86EE213 pKa = 3.59EE214 pKa = 4.51AKK216 pKa = 10.79YY217 pKa = 10.68EE218 pKa = 4.05NHH220 pKa = 7.03LEE222 pKa = 4.07NALMLYY228 pKa = 9.88SASSHH233 pKa = 6.23ASNPVYY239 pKa = 9.46QTLRR243 pKa = 11.84CRR245 pKa = 11.84AYY247 pKa = 10.58FYY249 pKa = 10.72DD250 pKa = 3.51SHH252 pKa = 7.95NNN254 pKa = 3.3

Molecular weight: 29.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1119

85

364

186.5

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.004 ± 0.724

2.949 ± 0.562

5.094 ± 0.53

4.826 ± 0.593

4.111 ± 0.641

6.166 ± 0.475

3.396 ± 0.291

5.094 ± 0.577

6.166 ± 0.58

8.132 ± 1.324

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.966 ± 0.5

4.379 ± 0.589

6.613 ± 0.829

4.558 ± 0.805

6.792 ± 1.256

8.132 ± 0.96

5.987 ± 1.244

5.63 ± 0.767

1.609 ± 0.235

3.396 ± 0.564
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski