Bacillus sp. HNG

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371S6T7|A0A371S6T7_9BACI Alpha/beta hydrolase OS=Bacillus sp. HNG OX=2293325 GN=DZB84_17255 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.17SISVSFHH9 pKa = 6.6KK10 pKa = 10.56EE11 pKa = 3.58DD12 pKa = 3.19AVEE15 pKa = 4.04AMKK18 pKa = 10.82VDD20 pKa = 3.26ILEE23 pKa = 4.45EE24 pKa = 3.92EE25 pKa = 4.26DD26 pKa = 3.77FNRR29 pKa = 11.84VTEE32 pKa = 4.16GGTRR36 pKa = 11.84HH37 pKa = 6.61LFEE40 pKa = 6.96LDD42 pKa = 3.3TNIGFFVFFDD52 pKa = 4.19GEE54 pKa = 4.12DD55 pKa = 3.49AEE57 pKa = 5.46GNIHH61 pKa = 6.46YY62 pKa = 9.93LALQYY67 pKa = 10.69EE68 pKa = 4.88EE69 pKa = 5.04EE70 pKa = 4.69SEE72 pKa = 4.18DD73 pKa = 3.39PSYY76 pKa = 11.4CFEE79 pKa = 4.56FEE81 pKa = 4.89LKK83 pKa = 10.65DD84 pKa = 4.0FYY86 pKa = 11.58EE87 pKa = 5.48FMALYY92 pKa = 10.86LNDD95 pKa = 4.88LEE97 pKa = 5.29FSDD100 pKa = 4.72EE101 pKa = 4.02EE102 pKa = 4.81SEE104 pKa = 5.12GEE106 pKa = 4.0EE107 pKa = 3.95QEE109 pKa = 4.39YY110 pKa = 11.05GPIHH114 pKa = 6.48HH115 pKa = 7.27LAHH118 pKa = 6.89LLYY121 pKa = 10.71HH122 pKa = 6.17IVEE125 pKa = 4.66DD126 pKa = 4.29AKK128 pKa = 11.03QAA130 pKa = 3.42

Molecular weight: 15.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371RYP9|A0A371RYP9_9BACI Ribosomal RNA small subunit methyltransferase G OS=Bacillus sp. HNG OX=2293325 GN=rsmG PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.1RR10 pKa = 11.84KK11 pKa = 9.07HH12 pKa = 5.9SKK14 pKa = 8.51VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.57NGRR28 pKa = 11.84KK29 pKa = 8.81VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4747

0

4747

1377384

14

1663

290.2

32.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.556 ± 0.04

0.677 ± 0.01

5.049 ± 0.025

7.556 ± 0.043

4.74 ± 0.033

6.937 ± 0.039

2.068 ± 0.015

8.168 ± 0.039

6.966 ± 0.033

9.67 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.676 ± 0.017

4.623 ± 0.023

3.621 ± 0.021

3.57 ± 0.021

3.843 ± 0.023

5.95 ± 0.022

5.514 ± 0.024

7.12 ± 0.034

1.063 ± 0.015

3.633 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski