Amino acid dipepetide frequency for Mycobacterium phage Pinnie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.956AlaAla: 18.956 ± 1.673
0.483AlaCys: 0.483 ± 0.204
8.134AlaAsp: 8.134 ± 0.88
8.823AlaGlu: 8.823 ± 1.443
4.481AlaPhe: 4.481 ± 0.714
14.2AlaGly: 14.2 ± 1.557
2.137AlaHis: 2.137 ± 0.388
7.169AlaIle: 7.169 ± 0.725
5.239AlaLys: 5.239 ± 0.65
12.132AlaLeu: 12.132 ± 1.191
3.24AlaMet: 3.24 ± 0.665
3.998AlaAsn: 3.998 ± 0.575
6.48AlaPro: 6.48 ± 0.724
5.032AlaGln: 5.032 ± 0.584
7.238AlaArg: 7.238 ± 0.852
4.894AlaSer: 4.894 ± 0.911
6.893AlaThr: 6.893 ± 0.726
7.858AlaVal: 7.858 ± 0.692
1.93AlaTrp: 1.93 ± 0.341
2.068AlaTyr: 2.068 ± 0.368
0.0AlaXaa: 0.0 ± 0.0
Cys
0.758CysAla: 0.758 ± 0.246
0.069CysCys: 0.069 ± 0.061
0.62CysAsp: 0.62 ± 0.245
0.827CysGlu: 0.827 ± 0.24
0.345CysPhe: 0.345 ± 0.132
1.448CysGly: 1.448 ± 0.406
0.138CysHis: 0.138 ± 0.098
0.414CysIle: 0.414 ± 0.15
0.138CysLys: 0.138 ± 0.085
0.414CysLeu: 0.414 ± 0.15
0.207CysMet: 0.207 ± 0.123
0.207CysAsn: 0.207 ± 0.093
0.827CysPro: 0.827 ± 0.31
0.414CysGln: 0.414 ± 0.167
1.517CysArg: 1.517 ± 0.324
0.345CysSer: 0.345 ± 0.152
0.62CysThr: 0.62 ± 0.28
0.62CysVal: 0.62 ± 0.237
0.207CysTrp: 0.207 ± 0.157
0.207CysTyr: 0.207 ± 0.125
0.0CysXaa: 0.0 ± 0.0
Asp
9.375AspAla: 9.375 ± 1.34
1.103AspCys: 1.103 ± 0.351
7.72AspAsp: 7.72 ± 0.905
5.032AspGlu: 5.032 ± 0.673
1.93AspPhe: 1.93 ± 0.445
6.893AspGly: 6.893 ± 0.724
1.448AspHis: 1.448 ± 0.3
0.827AspIle: 0.827 ± 0.24
1.448AspLys: 1.448 ± 0.394
4.55AspLeu: 4.55 ± 0.567
1.241AspMet: 1.241 ± 0.261
1.585AspAsn: 1.585 ± 0.338
4.412AspPro: 4.412 ± 0.687
3.378AspGln: 3.378 ± 0.527
5.239AspArg: 5.239 ± 0.635
1.861AspSer: 1.861 ± 0.44
3.86AspThr: 3.86 ± 0.48
5.652AspVal: 5.652 ± 0.621
1.861AspTrp: 1.861 ± 0.299
2.619AspTyr: 2.619 ± 0.463
0.0AspXaa: 0.0 ± 0.0
Glu
7.238GluAla: 7.238 ± 0.998
0.483GluCys: 0.483 ± 0.175
3.998GluAsp: 3.998 ± 0.672
3.722GluGlu: 3.722 ± 0.657
1.723GluPhe: 1.723 ± 0.387
3.447GluGly: 3.447 ± 0.352
2.137GluHis: 2.137 ± 0.355
1.999GluIle: 1.999 ± 0.384
2.55GluLys: 2.55 ± 0.425
3.86GluLeu: 3.86 ± 0.482
0.965GluMet: 0.965 ± 0.305
1.654GluAsn: 1.654 ± 0.325
3.86GluPro: 3.86 ± 0.778
2.895GluGln: 2.895 ± 0.495
5.239GluArg: 5.239 ± 0.959
1.448GluSer: 1.448 ± 0.242
2.757GluThr: 2.757 ± 0.431
4.136GluVal: 4.136 ± 0.625
1.172GluTrp: 1.172 ± 0.264
1.241GluTyr: 1.241 ± 0.31
0.0GluXaa: 0.0 ± 0.0
Phe
3.791PheAla: 3.791 ± 0.49
0.551PheCys: 0.551 ± 0.213
2.688PheAsp: 2.688 ± 0.49
2.826PheGlu: 2.826 ± 0.486
0.483PhePhe: 0.483 ± 0.173
3.309PheGly: 3.309 ± 0.508
0.551PheHis: 0.551 ± 0.217
0.62PheIle: 0.62 ± 0.194
1.172PheLys: 1.172 ± 0.331
1.861PheLeu: 1.861 ± 0.402
0.62PheMet: 0.62 ± 0.179
0.965PheAsn: 0.965 ± 0.262
1.379PhePro: 1.379 ± 0.284
0.896PheGln: 0.896 ± 0.252
1.31PheArg: 1.31 ± 0.283
1.861PheSer: 1.861 ± 0.305
1.585PheThr: 1.585 ± 0.461
2.275PheVal: 2.275 ± 0.436
0.62PheTrp: 0.62 ± 0.282
0.896PheTyr: 0.896 ± 0.286
0.0PheXaa: 0.0 ± 0.0
Gly
11.167GlyAla: 11.167 ± 1.563
0.758GlyCys: 0.758 ± 0.205
7.169GlyAsp: 7.169 ± 0.655
3.653GlyGlu: 3.653 ± 0.415
3.309GlyPhe: 3.309 ± 0.589
12.201GlyGly: 12.201 ± 3.576
1.93GlyHis: 1.93 ± 0.429
4.205GlyIle: 4.205 ± 0.542
4.274GlyLys: 4.274 ± 0.723
6.549GlyLeu: 6.549 ± 0.771
1.861GlyMet: 1.861 ± 0.336
3.791GlyAsn: 3.791 ± 0.572
3.791GlyPro: 3.791 ± 0.632
2.482GlyGln: 2.482 ± 0.462
5.721GlyArg: 5.721 ± 0.593
4.825GlySer: 4.825 ± 0.776
6.066GlyThr: 6.066 ± 0.746
5.584GlyVal: 5.584 ± 0.955
1.999GlyTrp: 1.999 ± 0.384
2.895GlyTyr: 2.895 ± 0.577
0.0GlyXaa: 0.0 ± 0.0
His
2.068HisAla: 2.068 ± 0.517
0.276HisCys: 0.276 ± 0.137
1.172HisAsp: 1.172 ± 0.288
0.551HisGlu: 0.551 ± 0.196
0.483HisPhe: 0.483 ± 0.167
2.137HisGly: 2.137 ± 0.375
0.138HisHis: 0.138 ± 0.112
0.689HisIle: 0.689 ± 0.264
0.276HisLys: 0.276 ± 0.123
1.241HisLeu: 1.241 ± 0.303
0.62HisMet: 0.62 ± 0.197
0.689HisAsn: 0.689 ± 0.218
1.654HisPro: 1.654 ± 0.399
0.965HisGln: 0.965 ± 0.232
2.137HisArg: 2.137 ± 0.457
1.172HisSer: 1.172 ± 0.326
1.103HisThr: 1.103 ± 0.287
1.999HisVal: 1.999 ± 0.326
0.345HisTrp: 0.345 ± 0.243
0.414HisTyr: 0.414 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
6.755IleAla: 6.755 ± 0.594
0.414IleCys: 0.414 ± 0.18
3.653IleAsp: 3.653 ± 0.505
2.344IleGlu: 2.344 ± 0.488
0.758IlePhe: 0.758 ± 0.25
4.756IleGly: 4.756 ± 0.917
0.965IleHis: 0.965 ± 0.245
1.517IleIle: 1.517 ± 0.341
0.896IleLys: 0.896 ± 0.315
2.55IleLeu: 2.55 ± 0.329
0.62IleMet: 0.62 ± 0.266
0.896IleAsn: 0.896 ± 0.35
3.033IlePro: 3.033 ± 0.504
1.241IleGln: 1.241 ± 0.256
3.516IleArg: 3.516 ± 0.505
1.517IleSer: 1.517 ± 0.372
2.068IleThr: 2.068 ± 0.432
2.826IleVal: 2.826 ± 0.329
0.896IleTrp: 0.896 ± 0.237
0.62IleTyr: 0.62 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
5.032LysAla: 5.032 ± 0.612
0.345LysCys: 0.345 ± 0.139
1.517LysAsp: 1.517 ± 0.415
0.896LysGlu: 0.896 ± 0.29
1.172LysPhe: 1.172 ± 0.253
2.206LysGly: 2.206 ± 0.437
0.827LysHis: 0.827 ± 0.255
1.861LysIle: 1.861 ± 0.358
0.965LysLys: 0.965 ± 0.315
2.55LysLeu: 2.55 ± 0.385
1.172LysMet: 1.172 ± 0.242
0.896LysAsn: 0.896 ± 0.257
2.757LysPro: 2.757 ± 0.443
0.758LysGln: 0.758 ± 0.258
3.447LysArg: 3.447 ± 0.637
1.723LysSer: 1.723 ± 0.417
2.619LysThr: 2.619 ± 0.543
2.275LysVal: 2.275 ± 0.473
0.483LysTrp: 0.483 ± 0.134
0.689LysTyr: 0.689 ± 0.236
0.0LysXaa: 0.0 ± 0.0
Leu
8.203LeuAla: 8.203 ± 1.028
0.62LeuCys: 0.62 ± 0.208
5.928LeuAsp: 5.928 ± 0.697
3.929LeuGlu: 3.929 ± 0.552
2.619LeuPhe: 2.619 ± 0.418
7.651LeuGly: 7.651 ± 1.637
1.034LeuHis: 1.034 ± 0.255
2.826LeuIle: 2.826 ± 0.431
2.55LeuLys: 2.55 ± 0.542
5.79LeuLeu: 5.79 ± 0.606
1.379LeuMet: 1.379 ± 0.45
2.275LeuAsn: 2.275 ± 0.363
4.067LeuPro: 4.067 ± 0.698
1.517LeuGln: 1.517 ± 0.301
5.515LeuArg: 5.515 ± 0.504
4.412LeuSer: 4.412 ± 0.551
5.584LeuThr: 5.584 ± 0.661
4.963LeuVal: 4.963 ± 0.691
1.31LeuTrp: 1.31 ± 0.332
1.792LeuTyr: 1.792 ± 0.43
0.0LeuXaa: 0.0 ± 0.0
Met
3.033MetAla: 3.033 ± 0.526
0.207MetCys: 0.207 ± 0.103
1.241MetAsp: 1.241 ± 0.306
0.827MetGlu: 0.827 ± 0.185
0.62MetPhe: 0.62 ± 0.183
1.379MetGly: 1.379 ± 0.313
0.345MetHis: 0.345 ± 0.137
1.172MetIle: 1.172 ± 0.299
0.827MetLys: 0.827 ± 0.179
1.517MetLeu: 1.517 ± 0.317
0.345MetMet: 0.345 ± 0.145
0.483MetAsn: 0.483 ± 0.162
1.448MetPro: 1.448 ± 0.313
0.689MetGln: 0.689 ± 0.218
1.172MetArg: 1.172 ± 0.26
1.448MetSer: 1.448 ± 0.308
2.55MetThr: 2.55 ± 0.38
1.172MetVal: 1.172 ± 0.307
0.207MetTrp: 0.207 ± 0.101
0.138MetTyr: 0.138 ± 0.094
0.0MetXaa: 0.0 ± 0.0
Asn
3.998AsnAla: 3.998 ± 0.592
0.414AsnCys: 0.414 ± 0.187
1.93AsnAsp: 1.93 ± 0.564
1.723AsnGlu: 1.723 ± 0.363
1.103AsnPhe: 1.103 ± 0.234
3.722AsnGly: 3.722 ± 0.59
0.689AsnHis: 0.689 ± 0.157
0.689AsnIle: 0.689 ± 0.207
0.758AsnLys: 0.758 ± 0.168
1.93AsnLeu: 1.93 ± 0.378
0.276AsnMet: 0.276 ± 0.135
0.896AsnAsn: 0.896 ± 0.25
4.067AsnPro: 4.067 ± 0.53
0.965AsnGln: 0.965 ± 0.353
2.826AsnArg: 2.826 ± 0.45
0.689AsnSer: 0.689 ± 0.285
1.93AsnThr: 1.93 ± 0.375
1.792AsnVal: 1.792 ± 0.456
0.758AsnTrp: 0.758 ± 0.192
0.483AsnTyr: 0.483 ± 0.153
0.0AsnXaa: 0.0 ± 0.0
Pro
9.513ProAla: 9.513 ± 0.893
0.276ProCys: 0.276 ± 0.135
5.652ProAsp: 5.652 ± 0.756
3.033ProGlu: 3.033 ± 0.468
1.585ProPhe: 1.585 ± 0.277
3.929ProGly: 3.929 ± 0.509
1.172ProHis: 1.172 ± 0.324
3.24ProIle: 3.24 ± 0.312
2.413ProLys: 2.413 ± 0.463
4.481ProLeu: 4.481 ± 0.518
1.379ProMet: 1.379 ± 0.346
2.482ProAsn: 2.482 ± 0.515
3.86ProPro: 3.86 ± 0.664
1.792ProGln: 1.792 ± 0.361
3.653ProArg: 3.653 ± 0.559
2.895ProSer: 2.895 ± 0.524
4.343ProThr: 4.343 ± 0.663
3.102ProVal: 3.102 ± 0.499
0.965ProTrp: 0.965 ± 0.237
1.103ProTyr: 1.103 ± 0.271
0.0ProXaa: 0.0 ± 0.0
Gln
5.239GlnAla: 5.239 ± 0.577
0.138GlnCys: 0.138 ± 0.106
1.861GlnAsp: 1.861 ± 0.376
0.896GlnGlu: 0.896 ± 0.241
1.172GlnPhe: 1.172 ± 0.271
2.619GlnGly: 2.619 ± 0.413
0.689GlnHis: 0.689 ± 0.173
1.585GlnIle: 1.585 ± 0.35
1.172GlnLys: 1.172 ± 0.31
4.343GlnLeu: 4.343 ± 0.562
1.448GlnMet: 1.448 ± 0.27
1.448GlnAsn: 1.448 ± 0.301
2.482GlnPro: 2.482 ± 0.32
1.654GlnGln: 1.654 ± 0.367
2.275GlnArg: 2.275 ± 0.344
1.31GlnSer: 1.31 ± 0.264
1.517GlnThr: 1.517 ± 0.274
1.654GlnVal: 1.654 ± 0.364
0.551GlnTrp: 0.551 ± 0.197
0.483GlnTyr: 0.483 ± 0.152
0.0GlnXaa: 0.0 ± 0.0
Arg
9.513ArgAla: 9.513 ± 0.999
1.448ArgCys: 1.448 ± 0.341
3.929ArgAsp: 3.929 ± 0.512
5.239ArgGlu: 5.239 ± 0.619
1.654ArgPhe: 1.654 ± 0.341
5.446ArgGly: 5.446 ± 0.571
1.654ArgHis: 1.654 ± 0.388
3.378ArgIle: 3.378 ± 0.399
2.482ArgLys: 2.482 ± 0.575
5.652ArgLeu: 5.652 ± 0.668
1.654ArgMet: 1.654 ± 0.28
2.344ArgAsn: 2.344 ± 0.474
3.86ArgPro: 3.86 ± 0.534
2.895ArgGln: 2.895 ± 0.642
7.376ArgArg: 7.376 ± 1.138
2.482ArgSer: 2.482 ± 0.296
4.894ArgThr: 4.894 ± 0.475
3.447ArgVal: 3.447 ± 0.398
1.103ArgTrp: 1.103 ± 0.354
1.723ArgTyr: 1.723 ± 0.371
0.0ArgXaa: 0.0 ± 0.0
Ser
4.963SerAla: 4.963 ± 0.637
0.483SerCys: 0.483 ± 0.255
3.722SerAsp: 3.722 ± 0.518
2.137SerGlu: 2.137 ± 0.32
1.241SerPhe: 1.241 ± 0.238
4.205SerGly: 4.205 ± 0.696
0.689SerHis: 0.689 ± 0.244
1.861SerIle: 1.861 ± 0.315
2.206SerLys: 2.206 ± 0.357
2.964SerLeu: 2.964 ± 0.626
0.62SerMet: 0.62 ± 0.197
1.585SerAsn: 1.585 ± 0.379
2.757SerPro: 2.757 ± 0.378
0.758SerGln: 0.758 ± 0.2
2.964SerArg: 2.964 ± 0.445
3.24SerSer: 3.24 ± 0.629
2.826SerThr: 2.826 ± 0.546
3.309SerVal: 3.309 ± 0.658
1.241SerTrp: 1.241 ± 0.322
1.103SerTyr: 1.103 ± 0.225
0.0SerXaa: 0.0 ± 0.0
Thr
9.099ThrAla: 9.099 ± 0.709
0.62ThrCys: 0.62 ± 0.23
4.136ThrAsp: 4.136 ± 0.598
3.447ThrGlu: 3.447 ± 0.491
2.275ThrPhe: 2.275 ± 0.484
5.515ThrGly: 5.515 ± 0.586
1.379ThrHis: 1.379 ± 0.269
2.757ThrIle: 2.757 ± 0.446
1.93ThrLys: 1.93 ± 0.349
3.791ThrLeu: 3.791 ± 0.529
1.585ThrMet: 1.585 ± 0.345
2.344ThrAsn: 2.344 ± 0.383
4.687ThrPro: 4.687 ± 0.53
1.31ThrGln: 1.31 ± 0.36
3.791ThrArg: 3.791 ± 0.451
3.86ThrSer: 3.86 ± 0.539
4.136ThrThr: 4.136 ± 0.566
3.791ThrVal: 3.791 ± 0.465
0.965ThrTrp: 0.965 ± 0.2
1.103ThrTyr: 1.103 ± 0.221
0.0ThrXaa: 0.0 ± 0.0
Val
8.134ValAla: 8.134 ± 1.234
1.172ValCys: 1.172 ± 0.316
5.032ValAsp: 5.032 ± 0.482
4.205ValGlu: 4.205 ± 0.526
1.93ValPhe: 1.93 ± 0.449
5.652ValGly: 5.652 ± 0.703
0.827ValHis: 0.827 ± 0.194
3.171ValIle: 3.171 ± 0.407
2.068ValLys: 2.068 ± 0.371
4.067ValLeu: 4.067 ± 0.569
1.172ValMet: 1.172 ± 0.314
1.792ValAsn: 1.792 ± 0.363
3.033ValPro: 3.033 ± 0.426
2.826ValGln: 2.826 ± 0.429
4.412ValArg: 4.412 ± 0.585
2.482ValSer: 2.482 ± 0.444
4.481ValThr: 4.481 ± 0.714
5.79ValVal: 5.79 ± 0.61
1.93ValTrp: 1.93 ± 0.442
1.585ValTyr: 1.585 ± 0.363
0.0ValXaa: 0.0 ± 0.0
Trp
1.861TrpAla: 1.861 ± 0.321
0.138TrpCys: 0.138 ± 0.095
0.965TrpAsp: 0.965 ± 0.227
1.103TrpGlu: 1.103 ± 0.24
0.896TrpPhe: 0.896 ± 0.227
1.654TrpGly: 1.654 ± 0.352
0.62TrpHis: 0.62 ± 0.22
1.103TrpIle: 1.103 ± 0.264
0.551TrpLys: 0.551 ± 0.201
2.068TrpLeu: 2.068 ± 0.495
0.276TrpMet: 0.276 ± 0.13
0.758TrpAsn: 0.758 ± 0.194
1.034TrpPro: 1.034 ± 0.223
0.827TrpGln: 0.827 ± 0.205
1.31TrpArg: 1.31 ± 0.327
1.172TrpSer: 1.172 ± 0.288
1.585TrpThr: 1.585 ± 0.397
0.965TrpVal: 0.965 ± 0.247
0.483TrpTrp: 0.483 ± 0.167
0.483TrpTyr: 0.483 ± 0.177
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.688TyrAla: 2.688 ± 0.508
0.345TyrCys: 0.345 ± 0.175
0.965TyrAsp: 0.965 ± 0.28
1.31TyrGlu: 1.31 ± 0.317
0.483TyrPhe: 0.483 ± 0.228
1.861TyrGly: 1.861 ± 0.387
0.758TyrHis: 0.758 ± 0.296
0.758TyrIle: 0.758 ± 0.256
0.345TyrLys: 0.345 ± 0.157
1.654TyrLeu: 1.654 ± 0.341
0.069TyrMet: 0.069 ± 0.06
0.551TyrAsn: 0.551 ± 0.197
1.379TyrPro: 1.379 ± 0.314
1.241TyrGln: 1.241 ± 0.239
1.517TyrArg: 1.517 ± 0.319
1.241TyrSer: 1.241 ± 0.409
1.103TyrThr: 1.103 ± 0.294
2.55TyrVal: 2.55 ± 0.434
0.827TyrTrp: 0.827 ± 0.27
0.414TyrTyr: 0.414 ± 0.165
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (14508 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski