Candidatus Nitrosopelagicus brevis

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Thaumarchaeota incertae sedis; Candidatus Nitrosopelagicus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1444 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7V239|A0A0A7V239_9ARCH Uncharacterized protein OS=Candidatus Nitrosopelagicus brevis OX=1410606 GN=A7X95_01605 PE=4 SV=1
MM1 pKa = 7.28TNCPEE6 pKa = 4.42CDD8 pKa = 3.57ANMKK12 pKa = 10.3IPDD15 pKa = 4.06DD16 pKa = 3.87AVEE19 pKa = 4.55GEE21 pKa = 4.36IVTCPEE27 pKa = 4.01CGASFEE33 pKa = 4.26LAKK36 pKa = 10.75ASEE39 pKa = 4.22GFEE42 pKa = 4.26LKK44 pKa = 10.11PAQTVGEE51 pKa = 4.3DD52 pKa = 2.73WGQQ55 pKa = 2.94

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7UYM8|A0A0A7UYM8_9ARCH 4-phosphopantoate--beta-alanine ligase OS=Candidatus Nitrosopelagicus brevis OX=1410606 GN=A7X95_04765 PE=3 SV=1
MM1 pKa = 7.4SKK3 pKa = 10.75AKK5 pKa = 10.3RR6 pKa = 11.84SAAAKK11 pKa = 9.55KK12 pKa = 9.82AARR15 pKa = 11.84TRR17 pKa = 11.84KK18 pKa = 9.29RR19 pKa = 11.84RR20 pKa = 11.84AAAAAAAATKK30 pKa = 10.13KK31 pKa = 10.01RR32 pKa = 11.84ASAARR37 pKa = 11.84KK38 pKa = 9.22RR39 pKa = 11.84KK40 pKa = 8.31RR41 pKa = 11.84TAAKK45 pKa = 10.2GGAKK49 pKa = 9.72RR50 pKa = 11.84KK51 pKa = 8.91AAPKK55 pKa = 10.07RR56 pKa = 11.84KK57 pKa = 9.17AAPKK61 pKa = 9.95RR62 pKa = 11.84KK63 pKa = 8.69AAKK66 pKa = 9.87RR67 pKa = 11.84KK68 pKa = 8.81AAPKK72 pKa = 10.02RR73 pKa = 11.84KK74 pKa = 8.69AAKK77 pKa = 9.87RR78 pKa = 11.84KK79 pKa = 8.81AAPKK83 pKa = 10.02RR84 pKa = 11.84KK85 pKa = 8.69AAKK88 pKa = 9.87RR89 pKa = 11.84KK90 pKa = 8.81AAPKK94 pKa = 10.02RR95 pKa = 11.84KK96 pKa = 8.69AAKK99 pKa = 9.87RR100 pKa = 11.84KK101 pKa = 8.81AAPKK105 pKa = 10.02RR106 pKa = 11.84KK107 pKa = 8.69AAKK110 pKa = 9.87RR111 pKa = 11.84KK112 pKa = 8.81AAPKK116 pKa = 10.02RR117 pKa = 11.84KK118 pKa = 8.69AAKK121 pKa = 9.87RR122 pKa = 11.84KK123 pKa = 8.81AAPKK127 pKa = 10.02RR128 pKa = 11.84KK129 pKa = 8.65AAKK132 pKa = 9.66RR133 pKa = 11.84KK134 pKa = 8.13APKK137 pKa = 9.43RR138 pKa = 11.84RR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 3.46

Molecular weight: 15.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1444

0

1444

377279

37

1409

261.3

29.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.056 ± 0.074

1.022 ± 0.031

5.86 ± 0.051

7.357 ± 0.071

4.444 ± 0.059

6.566 ± 0.058

1.76 ± 0.028

8.735 ± 0.062

8.849 ± 0.098

8.44 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.033

5.093 ± 0.055

3.69 ± 0.04

3.07 ± 0.037

3.511 ± 0.05

7.092 ± 0.062

5.429 ± 0.053

6.452 ± 0.053

0.886 ± 0.026

2.995 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski