Psychromonas sp. PRT-SC03
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 787 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P8Y8T9|A0A0P8Y8T9_9GAMM Uncharacterized protein OS=Psychromonas sp. PRT-SC03 OX=1527299 GN=JI57_00610 PE=4 SV=1
MM1 pKa = 7.45 PARR4 pKa = 11.84 RR5 pKa = 11.84 LLLLGIGNILYY16 pKa = 9.96 GDD18 pKa = 3.83 EE19 pKa = 4.82 GIGVHH24 pKa = 6.77 FINYY28 pKa = 9.56 ISDD31 pKa = 3.39 KK32 pKa = 11.16 YY33 pKa = 10.83 KK34 pKa = 10.42 IEE36 pKa = 4.15 NPAYY40 pKa = 9.99 EE41 pKa = 4.23 FDD43 pKa = 3.79 IVDD46 pKa = 4.38 GGTLAHH52 pKa = 6.75 GLIPLLATYY61 pKa = 10.7 DD62 pKa = 3.68 DD63 pKa = 5.52 LIIVDD68 pKa = 5.19 TINSADD74 pKa = 3.68 GEE76 pKa = 4.34 FGDD79 pKa = 5.29 VYY81 pKa = 11.04 FFDD84 pKa = 5.42 FDD86 pKa = 3.89 ATPADD91 pKa = 3.56
Molecular weight: 10.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.554
ProMoST 3.897
Dawson 3.795
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.732
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.91
Patrickios 0.846
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.849
Protein with the highest isoelectric point:
>tr|A0A0P8YU10|A0A0P8YU10_9GAMM HTH cro/C1-type domain-containing protein OS=Psychromonas sp. PRT-SC03 OX=1527299 GN=JI57_04605 PE=4 SV=1
RR1 pKa = 7.11 HH2 pKa = 6.07 RR3 pKa = 11.84 HH4 pKa = 5.73 SGRR7 pKa = 11.84 QLNRR11 pKa = 11.84 NSSHH15 pKa = 5.69 RR16 pKa = 11.84 QAMFRR21 pKa = 11.84 NMASSIVEE29 pKa = 3.93 HH30 pKa = 6.14 EE31 pKa = 4.62 VIKK34 pKa = 8.71 TTLPKK39 pKa = 10.57 AKK41 pKa = 9.36 EE42 pKa = 3.81 LRR44 pKa = 11.84 RR45 pKa = 11.84 GVEE48 pKa = 3.86 PLITLAKK55 pKa = 8.41 TDD57 pKa = 3.43 SVANRR62 pKa = 11.84 RR63 pKa = 11.84 LAFARR68 pKa = 11.84 TRR70 pKa = 11.84 DD71 pKa = 3.59 NAVVAKK77 pKa = 9.87 LFNEE81 pKa = 4.47 LGPRR85 pKa = 11.84 YY86 pKa = 9.78 ANRR89 pKa = 11.84 PGGYY93 pKa = 7.82 TRR95 pKa = 11.84 IITCGFSNGDD105 pKa = 4.05 KK106 pKa = 11.2 APMAYY111 pKa = 9.05 IEE113 pKa = 4.24 FVDD116 pKa = 3.84 RR117 pKa = 11.84 AVVADD122 pKa = 4.34 EE123 pKa = 4.13 EE124 pKa = 4.46 AA125 pKa = 3.99
Molecular weight: 14.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.575
IPC_protein 10.394
Toseland 10.628
ProMoST 10.584
Dawson 10.73
Bjellqvist 10.438
Wikipedia 10.935
Rodwell 10.891
Grimsley 10.774
Solomon 10.847
Lehninger 10.818
Nozaki 10.613
DTASelect 10.438
Thurlkill 10.628
EMBOSS 11.038
Sillero 10.657
Patrickios 10.643
IPC_peptide 10.862
IPC2_peptide 9.355
IPC2.peptide.svr19 8.768
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
787
0
787
213425
43
1903
271.2
30.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.946 ± 0.086
1.244 ± 0.037
5.304 ± 0.068
5.597 ± 0.103
4.231 ± 0.072
6.224 ± 0.095
2.236 ± 0.044
7.718 ± 0.078
6.8 ± 0.084
11.004 ± 0.111
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.044
4.781 ± 0.061
3.371 ± 0.052
4.626 ± 0.078
4.112 ± 0.082
6.767 ± 0.076
5.164 ± 0.059
6.277 ± 0.072
0.983 ± 0.029
3.06 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here