Anaeromyces robustus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Neocallimastigomycetes; Neocallimastigales; Neocallimastigaceae; Anaeromyces

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12666 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y1XGX6|A0A1Y1XGX6_9FUNG Lyase OS=Anaeromyces robustus OX=1754192 GN=BCR32DRAFT_262532 PE=3 SV=1
MM1 pKa = 7.46FFNKK5 pKa = 10.44GIFRR9 pKa = 11.84CEE11 pKa = 3.77IMDD14 pKa = 3.61MDD16 pKa = 4.32MDD18 pKa = 4.12INMEE22 pKa = 3.74IDD24 pKa = 3.1MDD26 pKa = 3.79IDD28 pKa = 3.22MDD30 pKa = 3.81MDD32 pKa = 3.49MDD34 pKa = 4.39MNMNMNNIDD43 pKa = 3.6MDD45 pKa = 3.67IDD47 pKa = 3.49INMDD51 pKa = 2.89MDD53 pKa = 3.72MDD55 pKa = 3.96MDD57 pKa = 3.95MDD59 pKa = 6.54RR60 pKa = 11.84IDD62 pKa = 4.13DD63 pKa = 4.18CNNFNQFQMGKK74 pKa = 9.57FLFLEE79 pKa = 4.66TVNLNYY85 pKa = 10.6YY86 pKa = 7.96MKK88 pKa = 10.51KK89 pKa = 9.72SYY91 pKa = 10.38YY92 pKa = 10.43NKK94 pKa = 10.34GSLLSYY100 pKa = 10.05HH101 pKa = 7.03IIISILNYY109 pKa = 10.21NKK111 pKa = 10.49CLDD114 pKa = 3.56IFDD117 pKa = 4.81SFGKK121 pKa = 10.33SSKK124 pKa = 10.75ACLNMIDD131 pKa = 3.83NDD133 pKa = 4.18YY134 pKa = 11.31NGLYY138 pKa = 9.89HH139 pKa = 7.58

Molecular weight: 16.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y1XAY2|A0A1Y1XAY2_9FUNG Pyridox_oxase_2 domain-containing protein OS=Anaeromyces robustus OX=1754192 GN=BCR32DRAFT_278527 PE=4 SV=1
MM1 pKa = 7.35NPNIPGMNPNMNPNMNNMNNMNNNNMHH28 pKa = 6.8SNMNPNINNMNNMNPNMNPNMNPNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNNMNMGMGMNMNQNRR122 pKa = 11.84RR123 pKa = 11.84MNRR126 pKa = 11.84NMNRR130 pKa = 11.84NMNRR134 pKa = 11.84NRR136 pKa = 11.84NMNFNKK142 pKa = 10.4RR143 pKa = 11.84NDD145 pKa = 3.99RR146 pKa = 11.84NNMNMNNMNMNNMNMNNMGMNNMGMNNVGMNNMNMPINMNRR187 pKa = 11.84RR188 pKa = 11.84RR189 pKa = 11.84QNRR192 pKa = 3.23

Molecular weight: 22.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12656

10

12666

6486003

49

12326

512.1

58.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.483 ± 0.022

1.535 ± 0.017

5.887 ± 0.019

7.244 ± 0.04

4.178 ± 0.018

4.077 ± 0.024

1.648 ± 0.008

8.854 ± 0.031

8.979 ± 0.026

8.077 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.931 ± 0.011

10.952 ± 0.078

3.42 ± 0.023

3.188 ± 0.02

3.075 ± 0.017

8.15 ± 0.033

5.512 ± 0.029

4.398 ± 0.029

0.757 ± 0.007

4.657 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski