Sweet potato pakakuy virus
Average proteome isoelectric point is 7.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C4NFM5|C4NFM5_9VIRU Reverse transcriptase OS=Sweet potato pakakuy virus OX=2034762 PE=4 SV=1
MM1 pKa = 7.68 RR2 pKa = 11.84 RR3 pKa = 11.84 PGAAAVTEE11 pKa = 4.2 VGLQGEE17 pKa = 4.6 GRR19 pKa = 11.84 NPQGEE24 pKa = 4.16 RR25 pKa = 11.84 QILHH29 pKa = 6.85 EE30 pKa = 4.34 DD31 pKa = 3.25 QIRR34 pKa = 11.84 DD35 pKa = 3.61 YY36 pKa = 11.41 RR37 pKa = 11.84 RR38 pKa = 11.84 MAEE41 pKa = 4.53 ARR43 pKa = 11.84 YY44 pKa = 9.09 QLQRR48 pKa = 11.84 QVARR52 pKa = 11.84 VLGRR56 pKa = 11.84 PYY58 pKa = 10.66 RR59 pKa = 11.84 RR60 pKa = 11.84 TLEE63 pKa = 4.08 RR64 pKa = 11.84 LMNPDD69 pKa = 3.1 QNLEE73 pKa = 4.04 DD74 pKa = 3.81 SLSRR78 pKa = 11.84 RR79 pKa = 11.84 ARR81 pKa = 11.84 IVPAEE86 pKa = 4.03 VLYY89 pKa = 11.15 SSTEE93 pKa = 3.74 GTEE96 pKa = 3.76 NQRR99 pKa = 11.84 VYY101 pKa = 10.23 IHH103 pKa = 6.96 RR104 pKa = 11.84 SEE106 pKa = 4.8 EE107 pKa = 4.28 EE108 pKa = 3.82 ITCLDD113 pKa = 3.73 NQQVDD118 pKa = 4.47 LPLITPQSHH127 pKa = 6.0 AQLLRR132 pKa = 11.84 QNYY135 pKa = 8.82 RR136 pKa = 11.84 FIHH139 pKa = 6.17 IGAIQVRR146 pKa = 11.84 VQALHH151 pKa = 5.92 RR152 pKa = 11.84 THH154 pKa = 7.22 AGTMVLVLNTDD165 pKa = 3.55 RR166 pKa = 11.84 RR167 pKa = 11.84 WNGDD171 pKa = 2.88 LSLFGGIEE179 pKa = 4.06 GDD181 pKa = 3.55 LTEE184 pKa = 4.75 GAFMTYY190 pKa = 10.03 IIPNVTMTVEE200 pKa = 4.79 DD201 pKa = 4.63 FCQNIMVEE209 pKa = 4.41 FQTRR213 pKa = 11.84 GYY215 pKa = 9.5 SEE217 pKa = 4.18 WVHH220 pKa = 6.03 GSNLLITRR228 pKa = 11.84 GMVGRR233 pKa = 11.84 LSNTPNVGFNYY244 pKa = 10.03 NVSAVTDD251 pKa = 3.71 YY252 pKa = 11.22 LVSKK256 pKa = 9.63 GVRR259 pKa = 11.84 ALPGRR264 pKa = 11.84 RR265 pKa = 11.84 YY266 pKa = 8.86 STADD270 pKa = 3.0 IQGLRR275 pKa = 11.84 WNVRR279 pKa = 11.84 RR280 pKa = 11.84 PRR282 pKa = 11.84 EE283 pKa = 4.08 IIPRR287 pKa = 11.84 RR288 pKa = 11.84 PTEE291 pKa = 4.06 MISRR295 pKa = 11.84 NLLGGGFSLSFRR307 pKa = 11.84 EE308 pKa = 4.14 YY309 pKa = 10.63 QPITAEE315 pKa = 3.77 QRR317 pKa = 11.84 RR318 pKa = 11.84 AAQHH322 pKa = 6.13 PEE324 pKa = 3.56 EE325 pKa = 5.07 DD326 pKa = 3.31 ALEE329 pKa = 4.23 EE330 pKa = 4.4 LEE332 pKa = 4.79 HH333 pKa = 6.41 EE334 pKa = 4.59 VLGVLDD340 pKa = 4.26 VEE342 pKa = 4.71 VADD345 pKa = 4.25 WDD347 pKa = 3.9 NLDD350 pKa = 4.12 FPEE353 pKa = 5.41 PDD355 pKa = 3.03 PTMVNIEE362 pKa = 4.02 IPEE365 pKa = 4.32 PEE367 pKa = 4.18 PQQPALEE374 pKa = 4.4 PVEE377 pKa = 4.69 HH378 pKa = 6.4 LTDD381 pKa = 3.36 EE382 pKa = 4.29 VLGFRR387 pKa = 11.84 RR388 pKa = 11.84 EE389 pKa = 3.99 ASSWNYY395 pKa = 10.26 LGSDD399 pKa = 3.73 SEE401 pKa = 4.5 EE402 pKa = 3.92 EE403 pKa = 3.88 ALLEE407 pKa = 3.7 FLYY410 pKa = 10.88 YY411 pKa = 10.7 RR412 pKa = 11.84 NTPDD416 pKa = 5.63 DD417 pKa = 3.94 GPYY420 pKa = 10.37 WNMEE424 pKa = 3.55 YY425 pKa = 11.41 SMAYY429 pKa = 8.6 QQLAAALEE437 pKa = 4.42 EE438 pKa = 4.44 DD439 pKa = 4.01 VSPNDD444 pKa = 4.41 VEE446 pKa = 5.45 SLDD449 pKa = 3.76 KK450 pKa = 10.84 NTVEE454 pKa = 5.27 GGTSTPFFGGEE465 pKa = 3.61 EE466 pKa = 3.84 AVPPKK471 pKa = 10.6 NSEE474 pKa = 4.06 NSCIMCNKK482 pKa = 9.96 KK483 pKa = 10.5 GIPGDD488 pKa = 4.22 KK489 pKa = 9.83 ILCQNCMDD497 pKa = 4.18 ITDD500 pKa = 4.55 DD501 pKa = 3.9 SDD503 pKa = 4.63 DD504 pKa = 3.5 EE505 pKa = 5.59 RR506 pKa = 11.84 EE507 pKa = 4.14 VQRR510 pKa = 11.84 QEE512 pKa = 3.3 RR513 pKa = 11.84 RR514 pKa = 11.84 KK515 pKa = 9.84 RR516 pKa = 11.84 MQRR519 pKa = 11.84 QKK521 pKa = 10.5 EE522 pKa = 4.08 KK523 pKa = 10.54 RR524 pKa = 11.84 VTPSTSDD531 pKa = 3.31 QTHH534 pKa = 6.43 HH535 pKa = 6.35 EE536 pKa = 4.19 VLGAINDD543 pKa = 3.95 EE544 pKa = 4.35 MPNTAEE550 pKa = 4.07 EE551 pKa = 4.18 FEE553 pKa = 4.46 EE554 pKa = 4.36 MVEE557 pKa = 3.84 RR558 pKa = 11.84 LYY560 pKa = 11.37 AQVSQQAPEE569 pKa = 4.14 ASNPAAPSTSRR580 pKa = 11.84 GQSAXSPSXPPEE592 pKa = 5.29 DD593 pKa = 3.42 ISMGQPSYY601 pKa = 11.55 APARR605 pKa = 11.84 PGTTTEE611 pKa = 3.57 ISGPPTFRR619 pKa = 11.84 TDD621 pKa = 2.77 SRR623 pKa = 11.84 FLKK626 pKa = 10.56 RR627 pKa = 11.84 GTNNEE632 pKa = 3.78 NWSLPPAQQQGGVLLTLPEE651 pKa = 4.08 QMGLLNDD658 pKa = 3.33 VFMRR662 pKa = 11.84 WEE664 pKa = 4.3 TTTLNHH670 pKa = 6.19 MSLMNIQDD678 pKa = 3.69 TQEE681 pKa = 3.99 KK682 pKa = 8.78 VDD684 pKa = 3.6 YY685 pKa = 10.02 MEE687 pKa = 4.8 NLLGEE692 pKa = 4.36 TAKK695 pKa = 10.79 LAWIQWRR702 pKa = 11.84 TVYY705 pKa = 10.48 EE706 pKa = 4.17 DD707 pKa = 3.62 EE708 pKa = 4.5 YY709 pKa = 10.65 KK710 pKa = 10.74 TIVGQAEE717 pKa = 4.2 GRR719 pKa = 11.84 MGTQNVISQVRR730 pKa = 11.84 RR731 pKa = 11.84 ILTLSDD737 pKa = 3.4 PVQGSTAVQDD747 pKa = 3.32 QAYY750 pKa = 9.93 RR751 pKa = 11.84 DD752 pKa = 3.99 LEE754 pKa = 3.99 RR755 pKa = 11.84 LQCNDD760 pKa = 2.89 VKK762 pKa = 11.76 DD763 pKa = 3.78 MVKK766 pKa = 10.47 FLNDD770 pKa = 3.43 YY771 pKa = 9.42 MRR773 pKa = 11.84 LATKK777 pKa = 9.62 TGRR780 pKa = 11.84 LYY782 pKa = 10.51 MGAEE786 pKa = 4.2 LSEE789 pKa = 4.31 KK790 pKa = 10.39 LWIKK794 pKa = 10.25 MPGDD798 pKa = 3.88 LGTKK802 pKa = 9.62 IKK804 pKa = 11.12 EE805 pKa = 4.5 EE806 pKa = 4.16 FNKK809 pKa = 10.21 AHH811 pKa = 7.37 PGAQIAVIPRR821 pKa = 11.84 IFFAHH826 pKa = 7.29 KK827 pKa = 10.15 YY828 pKa = 10.66 LEE830 pKa = 4.53 DD831 pKa = 3.67 RR832 pKa = 11.84 CKK834 pKa = 9.92 EE835 pKa = 3.78 AAFARR840 pKa = 11.84 SLKK843 pKa = 10.59 SVSFCKK849 pKa = 10.36 DD850 pKa = 2.72 IPIQGYY856 pKa = 9.38 YY857 pKa = 11.08 GNDD860 pKa = 3.15 KK861 pKa = 10.65 PKK863 pKa = 8.98 YY864 pKa = 7.29 TPRR867 pKa = 11.84 KK868 pKa = 8.22 ARR870 pKa = 11.84 TYY872 pKa = 9.96 KK873 pKa = 10.47 GKK875 pKa = 9.0 PHH877 pKa = 5.84 EE878 pKa = 4.09 THH880 pKa = 6.01 VRR882 pKa = 11.84 IDD884 pKa = 3.08 RR885 pKa = 11.84 RR886 pKa = 11.84 KK887 pKa = 8.51 NLDD890 pKa = 3.53 RR891 pKa = 11.84 NSHH894 pKa = 5.7 CKK896 pKa = 10.34 CFICEE901 pKa = 4.1 QPGHH905 pKa = 5.63 YY906 pKa = 10.53 ARR908 pKa = 11.84 DD909 pKa = 3.74 CPNQKK914 pKa = 10.37 RR915 pKa = 11.84 NINRR919 pKa = 11.84 VMMFNQVNIPDD930 pKa = 3.95 NYY932 pKa = 10.89 DD933 pKa = 3.06 IVSVSEE939 pKa = 3.81 NAEE942 pKa = 4.15 DD943 pKa = 3.56 SDD945 pKa = 4.94 AIYY948 pKa = 11.04 SLTEE952 pKa = 3.94 GDD954 pKa = 4.76 DD955 pKa = 3.85 AEE957 pKa = 4.36 EE958 pKa = 4.29 TNFGLVHH965 pKa = 6.65 EE966 pKa = 4.76 SVHH969 pKa = 6.21 MITHH973 pKa = 5.09 QVIGSWRR980 pKa = 11.84 AYY982 pKa = 9.52 IEE984 pKa = 3.95 PSEE987 pKa = 4.19 TQKK990 pKa = 10.47 VCRR993 pKa = 11.84 HH994 pKa = 5.12 QWQDD998 pKa = 3.01 HH999 pKa = 4.84 QEE1001 pKa = 3.9 IEE1003 pKa = 4.5 LPGEE1007 pKa = 4.58 DD1008 pKa = 2.9 TCLWCKK1014 pKa = 9.98 HH1015 pKa = 6.35 HH1016 pKa = 6.83 INIRR1020 pKa = 11.84 TRR1022 pKa = 11.84 SHH1024 pKa = 6.88 CPACLLTVCNICSLRR1039 pKa = 11.84 YY1040 pKa = 9.63 LGRR1043 pKa = 11.84 EE1044 pKa = 4.15 VPPKK1048 pKa = 9.49 AQEE1051 pKa = 3.95 RR1052 pKa = 11.84 VLPFPDD1058 pKa = 3.57 QSALIQQQQAYY1069 pKa = 7.96 MNWADD1074 pKa = 3.66 QDD1076 pKa = 3.75 RR1077 pKa = 11.84 ARR1079 pKa = 11.84 LKK1081 pKa = 10.83 QEE1083 pKa = 3.79 VNDD1086 pKa = 3.47 EE1087 pKa = 4.09 RR1088 pKa = 11.84 RR1089 pKa = 11.84 RR1090 pKa = 11.84 GQLLFEE1096 pKa = 4.38 EE1097 pKa = 4.69 EE1098 pKa = 3.54 RR1099 pKa = 11.84 RR1100 pKa = 11.84 RR1101 pKa = 11.84 AEE1103 pKa = 3.87 RR1104 pKa = 11.84 LGDD1107 pKa = 4.06 EE1108 pKa = 4.12 IAQLKK1113 pKa = 10.34 LRR1115 pKa = 11.84 MEE1117 pKa = 4.38 SMEE1120 pKa = 4.32 EE1121 pKa = 3.88 EE1122 pKa = 4.06 QKK1124 pKa = 11.1 LKK1126 pKa = 10.96 NDD1128 pKa = 4.81 LYY1130 pKa = 10.34 TQTEE1134 pKa = 4.07 KK1135 pKa = 11.07 DD1136 pKa = 3.35 LKK1138 pKa = 10.87 NRR1140 pKa = 11.84 IRR1142 pKa = 11.84 VLKK1145 pKa = 9.79 DD1146 pKa = 2.76 KK1147 pKa = 11.14 KK1148 pKa = 10.37 MEE1150 pKa = 4.35 LKK1152 pKa = 10.51 EE1153 pKa = 4.04 XLKK1156 pKa = 10.29 KK1157 pKa = 10.89 AKK1159 pKa = 6.61 XXRR1162 pKa = 11.84 YY1163 pKa = 7.59 EE1164 pKa = 3.93 ALEE1167 pKa = 4.2 KK1168 pKa = 10.29 EE1169 pKa = 4.52 RR1170 pKa = 11.84 KK1171 pKa = 9.28 SGRR1174 pKa = 11.84 KK1175 pKa = 6.39 VLKK1178 pKa = 9.14 MRR1180 pKa = 11.84 GVAPSLKK1187 pKa = 9.95 RR1188 pKa = 11.84 CC1189 pKa = 3.61
Molecular weight: 136.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.549
IPC2_protein 5.55
IPC_protein 5.563
Toseland 5.792
ProMoST 5.817
Dawson 5.715
Bjellqvist 5.766
Wikipedia 5.652
Rodwell 5.677
Grimsley 5.881
Solomon 5.715
Lehninger 5.69
Nozaki 5.919
DTASelect 6.084
Thurlkill 6.008
EMBOSS 5.957
Sillero 6.02
Patrickios 4.266
IPC_peptide 5.728
IPC2_peptide 6.02
IPC2.peptide.svr19 5.99
Protein with the highest isoelectric point:
>tr|C4NFM4|C4NFM4_9VIRU Polyprotein OS=Sweet potato pakakuy virus OX=2034762 PE=4 SV=1
MM1 pKa = 7.81 NYY3 pKa = 9.97 RR4 pKa = 11.84 EE5 pKa = 4.17 VQDD8 pKa = 3.81 LPGFKK13 pKa = 9.97 RR14 pKa = 11.84 ALTGTASLGAEE25 pKa = 4.41 GFNQPSGKK33 pKa = 7.67 TQSSLDD39 pKa = 3.37 TLIRR43 pKa = 11.84 QNNTLLFLAGVSDD56 pKa = 5.33 DD57 pKa = 4.17 RR58 pKa = 11.84 IQGIEE63 pKa = 4.03 EE64 pKa = 3.93 EE65 pKa = 4.21 LVEE68 pKa = 3.89 IRR70 pKa = 11.84 KK71 pKa = 9.82 AISKK75 pKa = 10.55 RR76 pKa = 11.84 EE77 pKa = 3.95 TPDD80 pKa = 2.97 LSGVVGQLQQLTIKK94 pKa = 10.52 GKK96 pKa = 9.22 APEE99 pKa = 4.02 PRR101 pKa = 11.84 GVLRR105 pKa = 11.84 VYY107 pKa = 10.53 QDD109 pKa = 3.7 PYY111 pKa = 10.42 LQTRR115 pKa = 11.84 RR116 pKa = 11.84 QINEE120 pKa = 3.71 EE121 pKa = 3.76 TRR123 pKa = 11.84 GRR125 pKa = 11.84 SSHH128 pKa = 6.57 RR129 pKa = 11.84 SRR131 pKa = 11.84 SSRR134 pKa = 11.84 GGSEE138 pKa = 4.07 PPRR141 pKa = 11.84 RR142 pKa = 11.84 TPDD145 pKa = 3.28 PPRR148 pKa = 11.84 GSDD151 pKa = 3.05 SS152 pKa = 3.29
Molecular weight: 16.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.124
IPC2_protein 9.136
IPC_protein 9.399
Toseland 10.043
ProMoST 9.706
Dawson 10.204
Bjellqvist 9.882
Wikipedia 10.394
Rodwell 10.438
Grimsley 10.277
Solomon 10.277
Lehninger 10.248
Nozaki 9.999
DTASelect 9.882
Thurlkill 10.072
EMBOSS 10.438
Sillero 10.116
Patrickios 10.145
IPC_peptide 10.277
IPC2_peptide 8.404
IPC2.peptide.svr19 8.319
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2470
142
1189
494.0
56.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.194 ± 0.813
1.457 ± 0.407
4.899 ± 0.521
8.785 ± 0.756
2.834 ± 0.508
5.951 ± 0.542
1.862 ± 0.27
6.113 ± 1.246
5.992 ± 1.333
9.109 ± 1.216
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.713 ± 0.429
4.656 ± 0.415
5.223 ± 0.39
5.709 ± 0.68
8.138 ± 0.982
5.911 ± 0.648
5.263 ± 0.229
4.818 ± 0.573
1.255 ± 0.156
2.874 ± 0.284
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here