Streptococcus phage Javan149

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A2I5|A0A4D6A2I5_9CAUD Recombinase OS=Streptococcus phage Javan149 OX=2548007 GN=Javan149_0047 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84KK3 pKa = 6.07TTEE6 pKa = 3.81EE7 pKa = 4.01EE8 pKa = 4.03KK9 pKa = 11.23VEE11 pKa = 4.54FSCDD15 pKa = 3.77EE16 pKa = 4.05VWDD19 pKa = 4.26GQIPEE24 pKa = 4.77LDD26 pKa = 3.75GDD28 pKa = 3.96VLIYY32 pKa = 10.93QNDD35 pKa = 4.18HH36 pKa = 6.46YY37 pKa = 11.55DD38 pKa = 3.68VDD40 pKa = 4.17CMTDD44 pKa = 2.79VDD46 pKa = 4.27TGIALYY52 pKa = 11.11NNDD55 pKa = 3.3YY56 pKa = 11.54DD57 pKa = 4.35DD58 pKa = 6.1FYY60 pKa = 10.37WCEE63 pKa = 3.79IEE65 pKa = 6.14APDD68 pKa = 4.42IGNGRR73 pKa = 11.84KK74 pKa = 8.96RR75 pKa = 3.32

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A0Q9|A0A4D6A0Q9_9CAUD Terminase small subunit OS=Streptococcus phage Javan149 OX=2548007 GN=Javan149_0030 PE=4 SV=1
MM1 pKa = 7.5TGMPALEE8 pKa = 4.07VKK10 pKa = 9.9LRR12 pKa = 11.84SMSEE16 pKa = 3.93KK17 pKa = 10.11RR18 pKa = 11.84WDD20 pKa = 3.41RR21 pKa = 11.84VVNKK25 pKa = 10.3NLTEE29 pKa = 3.92MFNRR33 pKa = 11.84SARR36 pKa = 11.84PPGTPIGKK44 pKa = 7.18NTKK47 pKa = 7.24RR48 pKa = 11.84HH49 pKa = 5.65KK50 pKa = 10.69SGEE53 pKa = 4.02LLRR56 pKa = 11.84SRR58 pKa = 11.84RR59 pKa = 11.84LKK61 pKa = 10.5KK62 pKa = 10.15VNSSKK67 pKa = 11.08GVITGNFGYY76 pKa = 10.24IKK78 pKa = 10.19DD79 pKa = 4.04YY80 pKa = 10.93APHH83 pKa = 6.57VEE85 pKa = 4.05YY86 pKa = 10.65GHH88 pKa = 6.96RR89 pKa = 11.84IVRR92 pKa = 11.84NGKK95 pKa = 9.1QIGYY99 pKa = 10.2VSGTKK104 pKa = 10.45YY105 pKa = 10.77LFNNVKK111 pKa = 9.38KK112 pKa = 10.39QRR114 pKa = 11.84EE115 pKa = 4.3IYY117 pKa = 9.23RR118 pKa = 11.84QDD120 pKa = 3.14MLNEE124 pKa = 3.91LRR126 pKa = 11.84RR127 pKa = 3.78

Molecular weight: 14.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

12384

39

1150

174.4

19.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.185 ± 0.37

0.541 ± 0.069

6.379 ± 0.287

7.332 ± 0.485

3.908 ± 0.194

6.226 ± 0.456

1.421 ± 0.127

6.985 ± 0.216

8.648 ± 0.435

8.495 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.193

6.177 ± 0.253

2.754 ± 0.115

3.803 ± 0.214

3.916 ± 0.231

6.613 ± 0.377

6.282 ± 0.423

6.468 ± 0.25

1.3 ± 0.105

4.191 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski