Staphylococcus phage Stab22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Kayvirus; unclassified Kayvirus

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A449C490|A0A449C490_9CAUD Putative tail fiber protein OS=Staphylococcus phage Stab22 OX=2490450 GN=g028c PE=4 SV=1
MM1 pKa = 7.58TNTIKK6 pKa = 10.72EE7 pKa = 4.09FLQTEE12 pKa = 4.24EE13 pKa = 4.16ASTVRR18 pKa = 11.84DD19 pKa = 3.7VATHH23 pKa = 5.4GVEE26 pKa = 3.84SGAIGRR32 pKa = 11.84LIYY35 pKa = 10.55TSDD38 pKa = 2.96VVNFFEE44 pKa = 6.55RR45 pKa = 11.84YY46 pKa = 7.78EE47 pKa = 3.93QDD49 pKa = 2.79IEE51 pKa = 4.64AVITNYY57 pKa = 10.95LEE59 pKa = 5.09GITGAQYY66 pKa = 11.5YY67 pKa = 10.78DD68 pKa = 3.59LLDD71 pKa = 3.8YY72 pKa = 11.41EE73 pKa = 4.61LMRR76 pKa = 11.84DD77 pKa = 3.44LEE79 pKa = 4.44EE80 pKa = 4.15YY81 pKa = 11.34SNVEE85 pKa = 3.66LFGEE89 pKa = 4.31EE90 pKa = 5.19EE91 pKa = 4.14YY92 pKa = 11.05TEE94 pKa = 4.19LQFDD98 pKa = 3.94LAEE101 pKa = 4.83NIASSEE107 pKa = 4.01IEE109 pKa = 4.09GFEE112 pKa = 5.26DD113 pKa = 4.15MDD115 pKa = 5.23EE116 pKa = 4.3EE117 pKa = 4.42DD118 pKa = 3.48QSEE121 pKa = 4.56AIYY124 pKa = 10.34EE125 pKa = 4.09AFNDD129 pKa = 3.8VEE131 pKa = 5.9LEE133 pKa = 3.96IQDD136 pKa = 3.48TDD138 pKa = 3.1KK139 pKa = 11.63VQFVNLAVEE148 pKa = 4.73IVAQEE153 pKa = 3.9LANII157 pKa = 4.3

Molecular weight: 18.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A449C4W0|A0A449C4W0_9CAUD Baseplate morphogenetic protein OS=Staphylococcus phage Stab22 OX=2490450 GN=g117 PE=4 SV=1
MM1 pKa = 7.55PVHH4 pKa = 6.96GKK6 pKa = 9.93RR7 pKa = 11.84PNLFKK12 pKa = 11.06NKK14 pKa = 9.6NYY16 pKa = 9.97KK17 pKa = 9.74QVGKK21 pKa = 8.94RR22 pKa = 11.84TIDD25 pKa = 3.47GMRR28 pKa = 11.84SEE30 pKa = 5.19VLDD33 pKa = 3.88KK34 pKa = 11.28LQATAQQVEE43 pKa = 4.3NTSIKK48 pKa = 10.49RR49 pKa = 11.84MPTYY53 pKa = 10.72LQVTEE58 pKa = 4.69KK59 pKa = 10.64KK60 pKa = 10.27LEE62 pKa = 4.05KK63 pKa = 10.66EE64 pKa = 4.25GVVDD68 pKa = 4.81LKK70 pKa = 11.17KK71 pKa = 10.96AFARR75 pKa = 11.84SSKK78 pKa = 10.67KK79 pKa = 9.14KK80 pKa = 8.16TSKK83 pKa = 10.92DD84 pKa = 3.25GGWYY88 pKa = 8.16LTVPIRR94 pKa = 11.84IKK96 pKa = 9.49TSRR99 pKa = 11.84MNNSTYY105 pKa = 10.89QDD107 pKa = 3.27MRR109 pKa = 11.84KK110 pKa = 9.89LKK112 pKa = 9.89VNQGTGSASKK122 pKa = 8.99ITDD125 pKa = 3.45YY126 pKa = 11.72LEE128 pKa = 4.1GRR130 pKa = 11.84RR131 pKa = 11.84KK132 pKa = 9.67NVSHH136 pKa = 7.49PSMKK140 pKa = 9.9PEE142 pKa = 4.09PMSHH146 pKa = 6.34NMTKK150 pKa = 10.19VKK152 pKa = 10.26RR153 pKa = 11.84GKK155 pKa = 9.51QSSYY159 pKa = 10.61FIFRR163 pKa = 11.84TVSSKK168 pKa = 11.09SPASSWILNRR178 pKa = 11.84DD179 pKa = 3.1KK180 pKa = 11.54VNEE183 pKa = 4.05NNFSKK188 pKa = 8.22TTLKK192 pKa = 9.76TVKK195 pKa = 10.24QLMNWKK201 pKa = 8.72MKK203 pKa = 10.32NLNN206 pKa = 3.45

Molecular weight: 23.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

206

5

211

42852

37

1352

203.1

23.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.231 ± 0.187

0.518 ± 0.051

7.09 ± 0.137

8.623 ± 0.256

4.063 ± 0.134

5.822 ± 0.293

1.61 ± 0.094

7.374 ± 0.178

8.751 ± 0.236

8.142 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.098

6.602 ± 0.156

2.735 ± 0.164

3.612 ± 0.152

3.584 ± 0.097

6.52 ± 0.182

6.06 ± 0.166

6.375 ± 0.17

0.835 ± 0.055

4.985 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski