Sulfurimonas sp. GYSZ_1
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2324 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5P8NYF1|A0A5P8NYF1_9PROT NifU family protein OS=Sulfurimonas sp. GYSZ_1 OX=2590022 GN=FJR48_01470 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 9.41 KK3 pKa = 8.4 TFYY6 pKa = 11.14 SLLILLSVFFTACGEE21 pKa = 4.17 NTKK24 pKa = 9.08 TAVVADD30 pKa = 3.69 IASIEE35 pKa = 4.07 IVEE38 pKa = 4.43 GNISIYY44 pKa = 9.9 STDD47 pKa = 3.5 EE48 pKa = 4.13 ATDD51 pKa = 3.67 LTAMATYY58 pKa = 9.9 TDD60 pKa = 3.94 STTAQINDD68 pKa = 3.26 AAIWNNSDD76 pKa = 3.43 YY77 pKa = 10.5 NTLYY81 pKa = 9.65 MYY83 pKa = 10.7 NGSVSAASNGGSSIVGISSGRR104 pKa = 11.84 FSDD107 pKa = 4.5 EE108 pKa = 3.9 INVSIIKK115 pKa = 8.92 LTDD118 pKa = 3.72 FNITNADD125 pKa = 3.42 INSTGSYY132 pKa = 9.73 ILEE135 pKa = 4.13 ATGTFEE141 pKa = 6.26 DD142 pKa = 4.15 NTSKK146 pKa = 10.84 VIKK149 pKa = 10.54 RR150 pKa = 11.84 NIEE153 pKa = 3.48 WSADD157 pKa = 3.16 NGAVISVDD165 pKa = 3.33 EE166 pKa = 4.92 NYY168 pKa = 10.59 VATITILTGDD178 pKa = 4.0 TNVTATVFEE187 pKa = 4.39 EE188 pKa = 4.81 TNTSAATLAPITRR201 pKa = 11.84 TYY203 pKa = 9.29 TVNN206 pKa = 3.03
Molecular weight: 22.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.808
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.795
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|A0A5P8NYX1|A0A5P8NYX1_9PROT Helix-turn-helix transcriptional regulator OS=Sulfurimonas sp. GYSZ_1 OX=2590022 GN=FJR48_02290 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 6.05 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.3 NGRR28 pKa = 11.84 NVLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.55 RR41 pKa = 11.84 LSVV44 pKa = 3.2
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2324
0
2324
743192
37
1982
319.8
36.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.345 ± 0.054
0.84 ± 0.019
6.182 ± 0.05
7.129 ± 0.053
4.894 ± 0.042
5.649 ± 0.052
1.851 ± 0.023
8.672 ± 0.052
8.93 ± 0.065
9.468 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.607 ± 0.024
5.703 ± 0.049
2.793 ± 0.028
2.839 ± 0.023
3.188 ± 0.028
6.927 ± 0.045
5.019 ± 0.044
6.124 ± 0.039
0.701 ± 0.018
4.139 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here