Amino acid dipepetide frequency for Lactococcus virus CB14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.564AlaAla: 0.564 ± 0.277
0.113AlaCys: 0.113 ± 0.119
2.93AlaAsp: 2.93 ± 0.593
4.621AlaGlu: 4.621 ± 0.827
3.156AlaPhe: 3.156 ± 0.728
4.621AlaGly: 4.621 ± 0.953
0.676AlaHis: 0.676 ± 0.276
5.184AlaIle: 5.184 ± 1.224
5.41AlaLys: 5.41 ± 0.946
5.973AlaLeu: 5.973 ± 0.999
2.254AlaMet: 2.254 ± 0.611
4.17AlaAsn: 4.17 ± 0.623
0.902AlaPro: 0.902 ± 0.386
2.592AlaGln: 2.592 ± 0.49
2.254AlaArg: 2.254 ± 0.552
3.945AlaSer: 3.945 ± 0.993
3.606AlaThr: 3.606 ± 0.69
3.494AlaVal: 3.494 ± 0.804
1.691AlaTrp: 1.691 ± 0.541
2.254AlaTyr: 2.254 ± 0.42
0.0AlaXaa: 0.0 ± 0.0
Cys
0.338CysAla: 0.338 ± 0.206
0.113CysCys: 0.113 ± 0.106
0.338CysAsp: 0.338 ± 0.186
0.338CysGlu: 0.338 ± 0.202
0.225CysPhe: 0.225 ± 0.181
0.902CysGly: 0.902 ± 0.362
0.225CysHis: 0.225 ± 0.166
0.338CysIle: 0.338 ± 0.208
1.014CysLys: 1.014 ± 0.382
0.451CysLeu: 0.451 ± 0.226
0.113CysMet: 0.113 ± 0.112
0.789CysAsn: 0.789 ± 0.279
0.338CysPro: 0.338 ± 0.251
0.225CysGln: 0.225 ± 0.147
0.676CysArg: 0.676 ± 0.29
0.338CysSer: 0.338 ± 0.182
0.225CysThr: 0.225 ± 0.156
0.451CysVal: 0.451 ± 0.218
0.113CysTrp: 0.113 ± 0.122
0.113CysTyr: 0.113 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
1.691AspAla: 1.691 ± 0.424
0.225AspCys: 0.225 ± 0.164
4.057AspAsp: 4.057 ± 0.927
4.057AspGlu: 4.057 ± 0.808
4.057AspPhe: 4.057 ± 0.691
4.057AspGly: 4.057 ± 0.701
1.014AspHis: 1.014 ± 0.405
4.621AspIle: 4.621 ± 0.848
4.733AspLys: 4.733 ± 0.642
6.537AspLeu: 6.537 ± 0.919
1.465AspMet: 1.465 ± 0.297
4.508AspAsn: 4.508 ± 0.71
1.24AspPro: 1.24 ± 0.495
0.676AspGln: 0.676 ± 0.293
1.578AspArg: 1.578 ± 0.531
2.93AspSer: 2.93 ± 0.537
4.395AspThr: 4.395 ± 0.825
3.268AspVal: 3.268 ± 0.595
0.789AspTrp: 0.789 ± 0.293
2.479AspTyr: 2.479 ± 0.546
0.0AspXaa: 0.0 ± 0.0
Glu
3.381GluAla: 3.381 ± 0.583
0.451GluCys: 0.451 ± 0.244
3.606GluAsp: 3.606 ± 0.768
4.283GluGlu: 4.283 ± 0.836
3.606GluPhe: 3.606 ± 0.591
2.141GluGly: 2.141 ± 0.433
0.902GluHis: 0.902 ± 0.309
5.973GluIle: 5.973 ± 0.925
5.522GluLys: 5.522 ± 1.012
9.467GluLeu: 9.467 ± 1.627
2.367GluMet: 2.367 ± 0.522
5.184GluAsn: 5.184 ± 0.92
1.352GluPro: 1.352 ± 0.419
3.832GluGln: 3.832 ± 0.822
2.254GluArg: 2.254 ± 0.521
3.945GluSer: 3.945 ± 0.483
4.621GluThr: 4.621 ± 0.778
3.381GluVal: 3.381 ± 0.548
1.014GluTrp: 1.014 ± 0.295
3.381GluTyr: 3.381 ± 0.725
0.0GluXaa: 0.0 ± 0.0
Phe
2.818PheAla: 2.818 ± 0.521
0.338PheCys: 0.338 ± 0.2
3.606PheAsp: 3.606 ± 0.795
3.043PheGlu: 3.043 ± 0.661
2.029PhePhe: 2.029 ± 0.676
2.254PheGly: 2.254 ± 0.483
0.225PheHis: 0.225 ± 0.169
3.832PheIle: 3.832 ± 0.768
4.508PheLys: 4.508 ± 0.699
2.029PheLeu: 2.029 ± 0.52
0.902PheMet: 0.902 ± 0.384
3.268PheAsn: 3.268 ± 0.823
0.902PhePro: 0.902 ± 0.295
1.24PheGln: 1.24 ± 0.39
0.789PheArg: 0.789 ± 0.235
4.283PheSer: 4.283 ± 1.25
3.268PheThr: 3.268 ± 0.443
2.705PheVal: 2.705 ± 0.508
0.338PheTrp: 0.338 ± 0.185
1.578PheTyr: 1.578 ± 0.363
0.0PheXaa: 0.0 ± 0.0
Gly
4.283GlyAla: 4.283 ± 1.587
0.338GlyCys: 0.338 ± 0.18
3.381GlyAsp: 3.381 ± 0.623
4.283GlyGlu: 4.283 ± 0.514
3.043GlyPhe: 3.043 ± 0.88
3.832GlyGly: 3.832 ± 0.69
0.564GlyHis: 0.564 ± 0.219
4.395GlyIle: 4.395 ± 1.358
6.987GlyLys: 6.987 ± 0.843
5.522GlyLeu: 5.522 ± 1.164
1.352GlyMet: 1.352 ± 0.376
3.606GlyAsn: 3.606 ± 0.704
0.451GlyPro: 0.451 ± 0.293
2.254GlyGln: 2.254 ± 0.559
1.691GlyArg: 1.691 ± 0.317
4.959GlySer: 4.959 ± 1.043
3.381GlyThr: 3.381 ± 0.946
5.973GlyVal: 5.973 ± 1.284
1.127GlyTrp: 1.127 ± 0.277
2.93GlyTyr: 2.93 ± 0.683
0.0GlyXaa: 0.0 ± 0.0
His
0.902HisAla: 0.902 ± 0.275
0.789HisCys: 0.789 ± 0.38
0.338HisAsp: 0.338 ± 0.176
0.564HisGlu: 0.564 ± 0.247
0.451HisPhe: 0.451 ± 0.215
1.014HisGly: 1.014 ± 0.44
0.113HisHis: 0.113 ± 0.123
1.014HisIle: 1.014 ± 0.281
0.676HisLys: 0.676 ± 0.292
1.014HisLeu: 1.014 ± 0.358
0.225HisMet: 0.225 ± 0.246
1.465HisAsn: 1.465 ± 0.455
0.225HisPro: 0.225 ± 0.136
0.225HisGln: 0.225 ± 0.143
0.225HisArg: 0.225 ± 0.149
0.338HisSer: 0.338 ± 0.18
1.014HisThr: 1.014 ± 0.343
1.127HisVal: 1.127 ± 0.411
0.0HisTrp: 0.0 ± 0.0
0.451HisTyr: 0.451 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
5.297IleAla: 5.297 ± 0.838
0.113IleCys: 0.113 ± 0.122
4.621IleAsp: 4.621 ± 0.595
6.311IleGlu: 6.311 ± 0.916
2.705IlePhe: 2.705 ± 0.639
3.719IleGly: 3.719 ± 0.924
1.127IleHis: 1.127 ± 0.425
5.297IleIle: 5.297 ± 0.782
6.875IleLys: 6.875 ± 0.956
5.522IleLeu: 5.522 ± 0.999
2.367IleMet: 2.367 ± 0.646
6.199IleAsn: 6.199 ± 0.728
1.691IlePro: 1.691 ± 0.407
2.479IleGln: 2.479 ± 0.381
1.916IleArg: 1.916 ± 0.411
3.606IleSer: 3.606 ± 0.67
4.508IleThr: 4.508 ± 0.671
4.508IleVal: 4.508 ± 0.82
1.465IleTrp: 1.465 ± 0.412
2.254IleTyr: 2.254 ± 0.433
0.0IleXaa: 0.0 ± 0.0
Lys
6.311LysAla: 6.311 ± 1.074
0.789LysCys: 0.789 ± 0.356
5.072LysAsp: 5.072 ± 0.62
7.889LysGlu: 7.889 ± 1.42
2.818LysPhe: 2.818 ± 0.636
5.635LysGly: 5.635 ± 0.981
1.24LysHis: 1.24 ± 0.444
4.959LysIle: 4.959 ± 0.906
8.903LysLys: 8.903 ± 1.258
7.1LysLeu: 7.1 ± 0.814
3.381LysMet: 3.381 ± 0.53
4.733LysAsn: 4.733 ± 0.689
1.691LysPro: 1.691 ± 0.484
3.381LysGln: 3.381 ± 0.775
3.494LysArg: 3.494 ± 0.823
5.748LysSer: 5.748 ± 0.959
5.748LysThr: 5.748 ± 0.911
5.635LysVal: 5.635 ± 0.894
1.352LysTrp: 1.352 ± 0.304
3.381LysTyr: 3.381 ± 0.716
0.0LysXaa: 0.0 ± 0.0
Leu
5.184LeuAla: 5.184 ± 0.604
0.338LeuCys: 0.338 ± 0.174
4.621LeuAsp: 4.621 ± 0.654
4.508LeuGlu: 4.508 ± 0.737
3.494LeuPhe: 3.494 ± 0.775
4.508LeuGly: 4.508 ± 0.682
1.352LeuHis: 1.352 ± 0.358
7.326LeuIle: 7.326 ± 0.995
8.453LeuLys: 8.453 ± 0.941
7.438LeuLeu: 7.438 ± 1.317
2.029LeuMet: 2.029 ± 0.706
5.635LeuAsn: 5.635 ± 0.985
3.268LeuPro: 3.268 ± 0.535
2.592LeuGln: 2.592 ± 0.477
2.818LeuArg: 2.818 ± 0.568
5.297LeuSer: 5.297 ± 0.755
6.086LeuThr: 6.086 ± 0.755
6.424LeuVal: 6.424 ± 0.718
1.127LeuTrp: 1.127 ± 0.313
4.846LeuTyr: 4.846 ± 0.975
0.0LeuXaa: 0.0 ± 0.0
Met
2.029MetAla: 2.029 ± 0.479
0.113MetCys: 0.113 ± 0.125
1.465MetAsp: 1.465 ± 0.375
1.352MetGlu: 1.352 ± 0.519
0.902MetPhe: 0.902 ± 0.389
1.24MetGly: 1.24 ± 0.319
0.451MetHis: 0.451 ± 0.237
2.592MetIle: 2.592 ± 0.565
2.367MetLys: 2.367 ± 0.496
2.141MetLeu: 2.141 ± 0.772
0.564MetMet: 0.564 ± 0.295
1.916MetAsn: 1.916 ± 0.439
0.676MetPro: 0.676 ± 0.281
1.465MetGln: 1.465 ± 0.364
0.564MetArg: 0.564 ± 0.303
1.578MetSer: 1.578 ± 0.347
2.254MetThr: 2.254 ± 0.595
1.691MetVal: 1.691 ± 0.358
0.338MetTrp: 0.338 ± 0.189
1.24MetTyr: 1.24 ± 0.412
0.0MetXaa: 0.0 ± 0.0
Asn
4.733AsnAla: 4.733 ± 0.984
0.338AsnCys: 0.338 ± 0.225
4.508AsnAsp: 4.508 ± 0.935
4.733AsnGlu: 4.733 ± 0.827
2.367AsnPhe: 2.367 ± 0.519
6.199AsnGly: 6.199 ± 0.818
1.127AsnHis: 1.127 ± 0.338
4.283AsnIle: 4.283 ± 0.651
6.649AsnLys: 6.649 ± 1.333
6.311AsnLeu: 6.311 ± 0.876
1.578AsnMet: 1.578 ± 0.361
3.832AsnAsn: 3.832 ± 0.818
2.479AsnPro: 2.479 ± 0.519
1.916AsnGln: 1.916 ± 0.533
1.803AsnArg: 1.803 ± 0.422
4.959AsnSer: 4.959 ± 0.7
3.494AsnThr: 3.494 ± 0.573
3.156AsnVal: 3.156 ± 0.527
1.127AsnTrp: 1.127 ± 0.44
2.592AsnTyr: 2.592 ± 0.514
0.0AsnXaa: 0.0 ± 0.0
Pro
1.465ProAla: 1.465 ± 0.493
0.113ProCys: 0.113 ± 0.096
1.916ProAsp: 1.916 ± 0.488
2.029ProGlu: 2.029 ± 0.568
1.014ProPhe: 1.014 ± 0.348
0.338ProGly: 0.338 ± 0.207
0.0ProHis: 0.0 ± 0.0
1.691ProIle: 1.691 ± 0.542
1.916ProLys: 1.916 ± 0.514
1.465ProLeu: 1.465 ± 0.344
0.564ProMet: 0.564 ± 0.238
2.479ProAsn: 2.479 ± 0.816
0.676ProPro: 0.676 ± 0.306
0.676ProGln: 0.676 ± 0.277
0.676ProArg: 0.676 ± 0.227
1.24ProSer: 1.24 ± 0.405
2.479ProThr: 2.479 ± 0.437
1.691ProVal: 1.691 ± 0.446
0.113ProTrp: 0.113 ± 0.106
1.24ProTyr: 1.24 ± 0.452
0.0ProXaa: 0.0 ± 0.0
Gln
3.494GlnAla: 3.494 ± 0.787
0.225GlnCys: 0.225 ± 0.149
1.352GlnAsp: 1.352 ± 0.477
2.254GlnGlu: 2.254 ± 0.625
1.014GlnPhe: 1.014 ± 0.308
2.592GlnGly: 2.592 ± 0.501
0.225GlnHis: 0.225 ± 0.16
1.578GlnIle: 1.578 ± 0.395
2.818GlnLys: 2.818 ± 0.624
3.494GlnLeu: 3.494 ± 0.55
0.676GlnMet: 0.676 ± 0.247
1.578GlnAsn: 1.578 ± 0.48
1.24GlnPro: 1.24 ± 0.435
1.352GlnGln: 1.352 ± 0.392
1.465GlnArg: 1.465 ± 0.46
1.916GlnSer: 1.916 ± 0.32
2.592GlnThr: 2.592 ± 0.498
2.141GlnVal: 2.141 ± 0.453
0.564GlnTrp: 0.564 ± 0.227
1.465GlnTyr: 1.465 ± 0.336
0.0GlnXaa: 0.0 ± 0.0
Arg
1.916ArgAla: 1.916 ± 0.601
0.451ArgCys: 0.451 ± 0.249
1.465ArgAsp: 1.465 ± 0.487
2.141ArgGlu: 2.141 ± 0.481
0.676ArgPhe: 0.676 ± 0.277
2.029ArgGly: 2.029 ± 0.561
0.564ArgHis: 0.564 ± 0.256
2.479ArgIle: 2.479 ± 0.535
4.057ArgLys: 4.057 ± 0.857
3.156ArgLeu: 3.156 ± 0.479
0.902ArgMet: 0.902 ± 0.36
2.254ArgAsn: 2.254 ± 0.631
0.564ArgPro: 0.564 ± 0.267
1.352ArgGln: 1.352 ± 0.335
1.916ArgArg: 1.916 ± 0.505
1.691ArgSer: 1.691 ± 0.366
1.465ArgThr: 1.465 ± 0.326
1.803ArgVal: 1.803 ± 0.466
0.451ArgTrp: 0.451 ± 0.191
1.803ArgTyr: 1.803 ± 0.399
0.0ArgXaa: 0.0 ± 0.0
Ser
4.395SerAla: 4.395 ± 1.178
0.676SerCys: 0.676 ± 0.298
3.381SerAsp: 3.381 ± 0.597
3.381SerGlu: 3.381 ± 0.778
2.818SerPhe: 2.818 ± 0.653
6.199SerGly: 6.199 ± 1.774
0.564SerHis: 0.564 ± 0.242
4.395SerIle: 4.395 ± 0.664
4.283SerLys: 4.283 ± 0.703
6.424SerLeu: 6.424 ± 0.885
2.141SerMet: 2.141 ± 0.356
4.17SerAsn: 4.17 ± 0.775
1.24SerPro: 1.24 ± 0.323
1.465SerGln: 1.465 ± 0.399
2.141SerArg: 2.141 ± 0.367
4.733SerSer: 4.733 ± 1.141
3.268SerThr: 3.268 ± 0.753
4.057SerVal: 4.057 ± 0.76
1.014SerTrp: 1.014 ± 0.455
2.254SerTyr: 2.254 ± 0.505
0.0SerXaa: 0.0 ± 0.0
Thr
5.072ThrAla: 5.072 ± 0.784
0.451ThrCys: 0.451 ± 0.207
3.832ThrAsp: 3.832 ± 0.844
5.41ThrGlu: 5.41 ± 0.725
3.043ThrPhe: 3.043 ± 0.693
5.297ThrGly: 5.297 ± 0.72
0.113ThrHis: 0.113 ± 0.147
4.17ThrIle: 4.17 ± 0.646
4.846ThrLys: 4.846 ± 0.595
5.748ThrLeu: 5.748 ± 0.724
1.014ThrMet: 1.014 ± 0.308
4.508ThrAsn: 4.508 ± 0.857
2.141ThrPro: 2.141 ± 0.417
2.705ThrGln: 2.705 ± 0.58
2.029ThrArg: 2.029 ± 0.48
4.057ThrSer: 4.057 ± 0.938
3.494ThrThr: 3.494 ± 0.57
5.072ThrVal: 5.072 ± 0.868
0.676ThrTrp: 0.676 ± 0.26
2.029ThrTyr: 2.029 ± 0.526
0.0ThrXaa: 0.0 ± 0.0
Val
3.832ValAla: 3.832 ± 0.537
0.676ValCys: 0.676 ± 0.342
4.733ValAsp: 4.733 ± 0.704
4.846ValGlu: 4.846 ± 0.625
2.818ValPhe: 2.818 ± 0.494
3.719ValGly: 3.719 ± 0.68
0.451ValHis: 0.451 ± 0.215
4.395ValIle: 4.395 ± 0.61
6.199ValLys: 6.199 ± 0.624
2.93ValLeu: 2.93 ± 0.469
1.691ValMet: 1.691 ± 0.43
3.494ValAsn: 3.494 ± 0.666
1.691ValPro: 1.691 ± 0.494
1.352ValGln: 1.352 ± 0.353
2.93ValArg: 2.93 ± 0.622
4.508ValSer: 4.508 ± 0.908
5.86ValThr: 5.86 ± 1.03
3.381ValVal: 3.381 ± 0.743
0.676ValTrp: 0.676 ± 0.321
2.367ValTyr: 2.367 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
1.127TrpAla: 1.127 ± 0.438
0.451TrpCys: 0.451 ± 0.214
0.902TrpAsp: 0.902 ± 0.363
0.564TrpGlu: 0.564 ± 0.229
1.24TrpPhe: 1.24 ± 0.428
0.676TrpGly: 0.676 ± 0.315
0.225TrpHis: 0.225 ± 0.161
0.676TrpIle: 0.676 ± 0.251
0.564TrpLys: 0.564 ± 0.261
1.465TrpLeu: 1.465 ± 0.443
0.338TrpMet: 0.338 ± 0.19
1.465TrpAsn: 1.465 ± 0.493
0.0TrpPro: 0.0 ± 0.0
0.902TrpGln: 0.902 ± 0.288
0.564TrpArg: 0.564 ± 0.266
1.127TrpSer: 1.127 ± 0.284
0.564TrpThr: 0.564 ± 0.233
0.451TrpVal: 0.451 ± 0.273
0.225TrpTrp: 0.225 ± 0.158
0.902TrpTyr: 0.902 ± 0.294
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.578TyrAla: 1.578 ± 0.45
0.564TyrCys: 0.564 ± 0.343
2.254TyrAsp: 2.254 ± 0.733
4.057TyrGlu: 4.057 ± 0.797
2.479TyrPhe: 2.479 ± 0.561
3.381TyrGly: 3.381 ± 0.65
1.014TyrHis: 1.014 ± 0.352
3.381TyrIle: 3.381 ± 0.835
2.592TyrLys: 2.592 ± 0.522
3.156TyrLeu: 3.156 ± 0.839
0.902TyrMet: 0.902 ± 0.299
2.93TyrAsn: 2.93 ± 0.565
1.014TyrPro: 1.014 ± 0.4
1.352TyrGln: 1.352 ± 0.542
1.352TyrArg: 1.352 ± 0.391
1.803TyrSer: 1.803 ± 0.458
3.381TyrThr: 3.381 ± 0.639
2.141TyrVal: 2.141 ± 0.422
0.225TyrTrp: 0.225 ± 0.187
1.916TyrTyr: 1.916 ± 0.467
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (8874 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski