Firmicutes bacterium CAG:308

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7A4S2|R7A4S2_9FIRM Penicillin V acylase and related amidases OS=Firmicutes bacterium CAG:308 OX=1263016 GN=BN599_01000 PE=3 SV=1
MM1 pKa = 7.43KK2 pKa = 10.37KK3 pKa = 9.86IFTFFISTFMILNLAACSSNNDD25 pKa = 3.26DD26 pKa = 3.67YY27 pKa = 12.08ALEE30 pKa = 4.35FFSALDD36 pKa = 3.37NTLEE40 pKa = 4.34LNSGHH45 pKa = 6.16IQGTFTSNNEE55 pKa = 3.89DD56 pKa = 3.07ASKK59 pKa = 10.76IKK61 pKa = 10.52FDD63 pKa = 3.9LQLNQKK69 pKa = 10.75DD70 pKa = 4.07NLQLALDD77 pKa = 4.74LDD79 pKa = 4.52LEE81 pKa = 5.24AGDD84 pKa = 3.84NAEE87 pKa = 4.94DD88 pKa = 3.67NFLNFYY94 pKa = 10.68IKK96 pKa = 10.5DD97 pKa = 3.32NKK99 pKa = 10.29TYY101 pKa = 10.45LNSYY105 pKa = 7.77GTTSQSLLEE114 pKa = 3.93NLGINGSEE122 pKa = 4.17KK123 pKa = 10.65LSVYY127 pKa = 10.82NPFLNYY133 pKa = 9.91TDD135 pKa = 5.72DD136 pKa = 3.62EE137 pKa = 4.59LKK139 pKa = 11.1ALFTRR144 pKa = 11.84SSKK147 pKa = 11.02DD148 pKa = 3.05GNNYY152 pKa = 9.92SFAIDD157 pKa = 3.73GSLISSYY164 pKa = 10.27LDD166 pKa = 3.01SMGSVTIEE174 pKa = 4.64DD175 pKa = 3.67ATLDD179 pKa = 3.42ATIEE183 pKa = 4.11DD184 pKa = 4.26NYY186 pKa = 9.6ITSLTLGISGLQDD199 pKa = 3.1VEE201 pKa = 4.45TQQVVIDD208 pKa = 3.72VTIEE212 pKa = 4.0CTLDD216 pKa = 3.3QINSLDD222 pKa = 3.82TVTYY226 pKa = 9.37PADD229 pKa = 3.68LEE231 pKa = 4.53NYY233 pKa = 9.49

Molecular weight: 25.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6ZU19|R6ZU19_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:308 OX=1263016 GN=BN599_00541 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.95RR10 pKa = 11.84KK11 pKa = 7.81HH12 pKa = 5.11QKK14 pKa = 8.48VHH16 pKa = 5.44GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1735

0

1735

515045

33

1537

296.9

33.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.138 ± 0.055

1.519 ± 0.021

6.188 ± 0.05

7.148 ± 0.055

4.388 ± 0.053

5.96 ± 0.059

2.002 ± 0.026

8.523 ± 0.057

7.704 ± 0.059

9.303 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.086 ± 0.031

5.04 ± 0.043

3.007 ± 0.03

3.65 ± 0.039

3.559 ± 0.039

6.012 ± 0.048

5.148 ± 0.047

6.556 ± 0.043

0.782 ± 0.019

4.286 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski