Amino acid dipepetide frequency for Streptomyces sp. NRRL WC-3605

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.275AlaAla: 21.275 ± 0.151
1.024AlaCys: 1.024 ± 0.025
8.335AlaAsp: 8.335 ± 0.061
8.912AlaGlu: 8.912 ± 0.091
3.496AlaPhe: 3.496 ± 0.042
13.254AlaGly: 13.254 ± 0.089
2.977AlaHis: 2.977 ± 0.039
3.321AlaIle: 3.321 ± 0.043
2.776AlaLys: 2.776 ± 0.05
14.84AlaLeu: 14.84 ± 0.124
2.452AlaMet: 2.452 ± 0.034
1.824AlaAsn: 1.824 ± 0.034
7.109AlaPro: 7.109 ± 0.086
3.71AlaGln: 3.71 ± 0.042
10.905AlaArg: 10.905 ± 0.086
5.941AlaSer: 5.941 ± 0.052
6.81AlaThr: 6.81 ± 0.065
12.52AlaVal: 12.52 ± 0.09
1.903AlaTrp: 1.903 ± 0.037
2.842AlaTyr: 2.842 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
1.073CysAla: 1.073 ± 0.023
0.083CysCys: 0.083 ± 0.007
0.458CysAsp: 0.458 ± 0.016
0.391CysGlu: 0.391 ± 0.015
0.211CysPhe: 0.211 ± 0.01
0.89CysGly: 0.89 ± 0.022
0.183CysHis: 0.183 ± 0.009
0.144CysIle: 0.144 ± 0.008
0.099CysLys: 0.099 ± 0.007
0.735CysLeu: 0.735 ± 0.018
0.111CysMet: 0.111 ± 0.008
0.118CysAsn: 0.118 ± 0.008
0.468CysPro: 0.468 ± 0.017
0.146CysGln: 0.146 ± 0.008
0.577CysArg: 0.577 ± 0.018
0.401CysSer: 0.401 ± 0.013
0.466CysThr: 0.466 ± 0.015
0.668CysVal: 0.668 ± 0.016
0.119CysTrp: 0.119 ± 0.007
0.141CysTyr: 0.141 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.045AspAla: 8.045 ± 0.07
0.412AspCys: 0.412 ± 0.015
3.914AspAsp: 3.914 ± 0.051
4.027AspGlu: 4.027 ± 0.045
1.637AspPhe: 1.637 ± 0.029
6.794AspGly: 6.794 ± 0.062
1.458AspHis: 1.458 ± 0.027
1.869AspIle: 1.869 ± 0.034
1.271AspLys: 1.271 ± 0.034
6.41AspLeu: 6.41 ± 0.055
0.84AspMet: 0.84 ± 0.021
0.947AspAsn: 0.947 ± 0.023
4.565AspPro: 4.565 ± 0.045
1.479AspGln: 1.479 ± 0.026
5.027AspArg: 5.027 ± 0.051
2.441AspSer: 2.441 ± 0.036
3.248AspThr: 3.248 ± 0.041
5.171AspVal: 5.171 ± 0.053
1.061AspTrp: 1.061 ± 0.025
1.074AspTyr: 1.074 ± 0.022
0.0AspXaa: 0.0 ± 0.0
Glu
7.651GluAla: 7.651 ± 0.08
0.354GluCys: 0.354 ± 0.014
3.109GluAsp: 3.109 ± 0.045
3.756GluGlu: 3.756 ± 0.061
1.447GluPhe: 1.447 ± 0.027
4.47GluGly: 4.47 ± 0.055
1.535GluHis: 1.535 ± 0.03
2.234GluIle: 2.234 ± 0.031
1.463GluLys: 1.463 ± 0.033
6.67GluLeu: 6.67 ± 0.073
0.909GluMet: 0.909 ± 0.02
1.026GluAsn: 1.026 ± 0.023
3.543GluPro: 3.543 ± 0.054
2.22GluGln: 2.22 ± 0.036
5.796GluArg: 5.796 ± 0.063
2.479GluSer: 2.479 ± 0.036
2.963GluThr: 2.963 ± 0.035
4.579GluVal: 4.579 ± 0.046
0.762GluTrp: 0.762 ± 0.019
1.109GluTyr: 1.109 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
3.625PheAla: 3.625 ± 0.042
0.256PheCys: 0.256 ± 0.009
1.948PheAsp: 1.948 ± 0.03
1.478PheGlu: 1.478 ± 0.03
0.863PhePhe: 0.863 ± 0.022
3.056PheGly: 3.056 ± 0.039
0.589PheHis: 0.589 ± 0.016
0.668PheIle: 0.668 ± 0.02
0.52PheLys: 0.52 ± 0.019
2.618PheLeu: 2.618 ± 0.041
0.419PheMet: 0.419 ± 0.015
0.522PheAsn: 0.522 ± 0.017
1.389PhePro: 1.389 ± 0.025
0.675PheGln: 0.675 ± 0.019
1.855PheArg: 1.855 ± 0.03
1.392PheSer: 1.392 ± 0.025
1.959PheThr: 1.959 ± 0.035
2.304PheVal: 2.304 ± 0.034
0.422PheTrp: 0.422 ± 0.014
0.565PheTyr: 0.565 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
11.203GlyAla: 11.203 ± 0.093
0.779GlyCys: 0.779 ± 0.022
5.527GlyAsp: 5.527 ± 0.069
5.236GlyGlu: 5.236 ± 0.061
2.926GlyPhe: 2.926 ± 0.04
8.921GlyGly: 8.921 ± 0.084
2.387GlyHis: 2.387 ± 0.035
3.276GlyIle: 3.276 ± 0.041
2.366GlyLys: 2.366 ± 0.042
9.596GlyLeu: 9.596 ± 0.078
1.971GlyMet: 1.971 ± 0.031
1.642GlyAsn: 1.642 ± 0.037
5.22GlyPro: 5.22 ± 0.056
2.605GlyGln: 2.605 ± 0.041
8.0GlyArg: 8.0 ± 0.064
5.153GlySer: 5.153 ± 0.059
6.531GlyThr: 6.531 ± 0.069
7.812GlyVal: 7.812 ± 0.068
1.737GlyTrp: 1.737 ± 0.031
2.26GlyTyr: 2.26 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
2.737HisAla: 2.737 ± 0.043
0.198HisCys: 0.198 ± 0.01
1.41HisAsp: 1.41 ± 0.025
1.262HisGlu: 1.262 ± 0.027
0.64HisPhe: 0.64 ± 0.016
2.463HisGly: 2.463 ± 0.034
0.654HisHis: 0.654 ± 0.017
0.675HisIle: 0.675 ± 0.019
0.349HisLys: 0.349 ± 0.014
2.417HisLeu: 2.417 ± 0.041
0.331HisMet: 0.331 ± 0.013
0.337HisAsn: 0.337 ± 0.013
1.861HisPro: 1.861 ± 0.033
0.589HisGln: 0.589 ± 0.017
2.125HisArg: 2.125 ± 0.031
0.954HisSer: 0.954 ± 0.02
1.371HisThr: 1.371 ± 0.027
1.77HisVal: 1.77 ± 0.036
0.381HisTrp: 0.381 ± 0.013
0.471HisTyr: 0.471 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.593IleAla: 4.593 ± 0.051
0.268IleCys: 0.268 ± 0.011
2.139IleAsp: 2.139 ± 0.041
1.905IleGlu: 1.905 ± 0.032
0.684IlePhe: 0.684 ± 0.02
3.45IleGly: 3.45 ± 0.044
0.576IleHis: 0.576 ± 0.015
0.832IleIle: 0.832 ± 0.024
0.706IleLys: 0.706 ± 0.02
2.335IleLeu: 2.335 ± 0.036
0.437IleMet: 0.437 ± 0.016
0.625IleAsn: 0.625 ± 0.018
1.693IlePro: 1.693 ± 0.033
0.703IleGln: 0.703 ± 0.017
2.136IleArg: 2.136 ± 0.032
1.509IleSer: 1.509 ± 0.026
2.128IleThr: 2.128 ± 0.032
2.754IleVal: 2.754 ± 0.042
0.357IleTrp: 0.357 ± 0.013
0.49IleTyr: 0.49 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
3.002LysAla: 3.002 ± 0.047
0.106LysCys: 0.106 ± 0.007
1.435LysAsp: 1.435 ± 0.037
1.218LysGlu: 1.218 ± 0.027
0.434LysPhe: 0.434 ± 0.016
1.912LysGly: 1.912 ± 0.038
0.406LysHis: 0.406 ± 0.013
0.829LysIle: 0.829 ± 0.024
0.88LysLys: 0.88 ± 0.034
1.922LysLeu: 1.922 ± 0.034
0.376LysMet: 0.376 ± 0.015
0.495LysAsn: 0.495 ± 0.019
1.308LysPro: 1.308 ± 0.032
0.687LysGln: 0.687 ± 0.02
1.436LysArg: 1.436 ± 0.031
1.092LysSer: 1.092 ± 0.029
1.237LysThr: 1.237 ± 0.029
1.983LysVal: 1.983 ± 0.039
0.257LysTrp: 0.257 ± 0.011
0.44LysTyr: 0.44 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
15.129LeuAla: 15.129 ± 0.112
0.828LeuCys: 0.828 ± 0.02
6.872LeuAsp: 6.872 ± 0.064
4.883LeuGlu: 4.883 ± 0.056
2.633LeuPhe: 2.633 ± 0.042
9.314LeuGly: 9.314 ± 0.071
2.288LeuHis: 2.288 ± 0.036
3.196LeuIle: 3.196 ± 0.041
2.086LeuLys: 2.086 ± 0.036
11.36LeuLeu: 11.36 ± 0.106
1.651LeuMet: 1.651 ± 0.027
1.61LeuAsn: 1.61 ± 0.024
6.416LeuPro: 6.416 ± 0.06
2.176LeuGln: 2.176 ± 0.03
8.775LeuArg: 8.775 ± 0.076
5.15LeuSer: 5.15 ± 0.055
6.88LeuThr: 6.88 ± 0.063
8.933LeuVal: 8.933 ± 0.078
1.28LeuTrp: 1.28 ± 0.028
1.874LeuTyr: 1.874 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.202MetAla: 2.202 ± 0.037
0.135MetCys: 0.135 ± 0.007
0.905MetAsp: 0.905 ± 0.018
0.779MetGlu: 0.779 ± 0.019
0.445MetPhe: 0.445 ± 0.016
1.338MetGly: 1.338 ± 0.022
0.347MetHis: 0.347 ± 0.014
0.636MetIle: 0.636 ± 0.02
0.416MetLys: 0.416 ± 0.012
1.605MetLeu: 1.605 ± 0.026
0.299MetMet: 0.299 ± 0.012
0.442MetAsn: 0.442 ± 0.014
1.128MetPro: 1.128 ± 0.025
0.424MetGln: 0.424 ± 0.016
1.446MetArg: 1.446 ± 0.026
1.322MetSer: 1.322 ± 0.023
1.555MetThr: 1.555 ± 0.029
1.264MetVal: 1.264 ± 0.027
0.22MetTrp: 0.22 ± 0.009
0.324MetTyr: 0.324 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.142AsnAla: 2.142 ± 0.034
0.156AsnCys: 0.156 ± 0.008
0.927AsnAsp: 0.927 ± 0.019
0.766AsnGlu: 0.766 ± 0.02
0.469AsnPhe: 0.469 ± 0.016
1.77AsnGly: 1.77 ± 0.036
0.377AsnHis: 0.377 ± 0.013
0.603AsnIle: 0.603 ± 0.018
0.392AsnLys: 0.392 ± 0.014
1.571AsnLeu: 1.571 ± 0.029
0.29AsnMet: 0.29 ± 0.012
0.398AsnAsn: 0.398 ± 0.015
1.279AsnPro: 1.279 ± 0.027
0.49AsnGln: 0.49 ± 0.016
1.21AsnArg: 1.21 ± 0.02
0.87AsnSer: 0.87 ± 0.023
1.024AsnThr: 1.024 ± 0.028
1.371AsnVal: 1.371 ± 0.029
0.269AsnTrp: 0.269 ± 0.011
0.375AsnTyr: 0.375 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.771ProAla: 8.771 ± 0.088
0.315ProCys: 0.315 ± 0.012
4.651ProAsp: 4.651 ± 0.052
4.463ProGlu: 4.463 ± 0.052
1.554ProPhe: 1.554 ± 0.028
6.736ProGly: 6.736 ± 0.069
1.433ProHis: 1.433 ± 0.025
1.184ProIle: 1.184 ± 0.023
1.16ProLys: 1.16 ± 0.029
5.351ProLeu: 5.351 ± 0.057
0.993ProMet: 0.993 ± 0.021
0.835ProAsn: 0.835 ± 0.022
3.501ProPro: 3.501 ± 0.054
1.706ProGln: 1.706 ± 0.041
4.083ProArg: 4.083 ± 0.053
3.178ProSer: 3.178 ± 0.043
3.028ProThr: 3.028 ± 0.043
5.483ProVal: 5.483 ± 0.051
0.897ProTrp: 0.897 ± 0.022
1.461ProTyr: 1.461 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.6GlnAla: 3.6 ± 0.039
0.156GlnCys: 0.156 ± 0.008
1.47GlnAsp: 1.47 ± 0.028
1.525GlnGlu: 1.525 ± 0.03
0.673GlnPhe: 0.673 ± 0.018
2.338GlnGly: 2.338 ± 0.039
0.592GlnHis: 0.592 ± 0.018
1.074GlnIle: 1.074 ± 0.025
0.611GlnLys: 0.611 ± 0.018
2.837GlnLeu: 2.837 ± 0.036
0.479GlnMet: 0.479 ± 0.015
0.476GlnAsn: 0.476 ± 0.017
1.639GlnPro: 1.639 ± 0.037
1.204GlnGln: 1.204 ± 0.038
2.241GlnArg: 2.241 ± 0.038
1.202GlnSer: 1.202 ± 0.028
1.301GlnThr: 1.301 ± 0.03
2.352GlnVal: 2.352 ± 0.034
0.428GlnTrp: 0.428 ± 0.014
0.618GlnTyr: 0.618 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
10.302ArgAla: 10.302 ± 0.098
0.54ArgCys: 0.54 ± 0.017
4.496ArgAsp: 4.496 ± 0.039
4.903ArgGlu: 4.903 ± 0.059
2.357ArgPhe: 2.357 ± 0.033
5.965ArgGly: 5.965 ± 0.055
2.224ArgHis: 2.224 ± 0.035
3.083ArgIle: 3.083 ± 0.032
1.638ArgLys: 1.638 ± 0.031
9.146ArgLeu: 9.146 ± 0.076
1.73ArgMet: 1.73 ± 0.029
1.262ArgAsn: 1.262 ± 0.025
5.146ArgPro: 5.146 ± 0.05
2.311ArgGln: 2.311 ± 0.034
8.105ArgArg: 8.105 ± 0.082
3.971ArgSer: 3.971 ± 0.047
5.547ArgThr: 5.547 ± 0.056
6.225ArgVal: 6.225 ± 0.052
1.449ArgTrp: 1.449 ± 0.026
1.834ArgTyr: 1.834 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.595SerAla: 6.595 ± 0.067
0.37SerCys: 0.37 ± 0.015
2.649SerAsp: 2.649 ± 0.035
2.399SerGlu: 2.399 ± 0.035
1.439SerPhe: 1.439 ± 0.027
5.792SerGly: 5.792 ± 0.058
0.975SerHis: 0.975 ± 0.023
1.332SerIle: 1.332 ± 0.029
1.021SerLys: 1.021 ± 0.024
4.72SerLeu: 4.72 ± 0.047
1.038SerMet: 1.038 ± 0.022
0.812SerAsn: 0.812 ± 0.02
3.055SerPro: 3.055 ± 0.035
1.211SerGln: 1.211 ± 0.024
3.612SerArg: 3.612 ± 0.043
2.672SerSer: 2.672 ± 0.039
2.924SerThr: 2.924 ± 0.039
4.225SerVal: 4.225 ± 0.042
0.872SerTrp: 0.872 ± 0.022
1.194SerTyr: 1.194 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
8.88ThrAla: 8.88 ± 0.072
0.413ThrCys: 0.413 ± 0.015
3.721ThrAsp: 3.721 ± 0.037
3.262ThrGlu: 3.262 ± 0.041
1.624ThrPhe: 1.624 ± 0.033
6.768ThrGly: 6.768 ± 0.062
1.237ThrHis: 1.237 ± 0.024
1.613ThrIle: 1.613 ± 0.033
1.157ThrLys: 1.157 ± 0.028
5.7ThrLeu: 5.7 ± 0.055
0.923ThrMet: 0.923 ± 0.021
0.939ThrAsn: 0.939 ± 0.024
4.11ThrPro: 4.11 ± 0.051
1.302ThrGln: 1.302 ± 0.028
4.066ThrArg: 4.066 ± 0.041
3.044ThrSer: 3.044 ± 0.038
3.668ThrThr: 3.668 ± 0.05
6.051ThrVal: 6.051 ± 0.062
0.903ThrTrp: 0.903 ± 0.024
1.348ThrTyr: 1.348 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
10.897ValAla: 10.897 ± 0.086
0.755ValCys: 0.755 ± 0.019
5.231ValAsp: 5.231 ± 0.045
4.887ValGlu: 4.887 ± 0.051
2.444ValPhe: 2.444 ± 0.036
6.504ValGly: 6.504 ± 0.074
2.016ValHis: 2.016 ± 0.032
2.725ValIle: 2.725 ± 0.042
1.784ValLys: 1.784 ± 0.033
9.691ValLeu: 9.691 ± 0.081
1.434ValMet: 1.434 ± 0.027
1.627ValAsn: 1.627 ± 0.028
5.503ValPro: 5.503 ± 0.061
2.007ValGln: 2.007 ± 0.032
7.624ValArg: 7.624 ± 0.064
4.298ValSer: 4.298 ± 0.041
5.851ValThr: 5.851 ± 0.052
8.4ValVal: 8.4 ± 0.076
1.216ValTrp: 1.216 ± 0.023
1.67ValTyr: 1.67 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.704TrpAla: 1.704 ± 0.028
0.157TrpCys: 0.157 ± 0.008
0.855TrpAsp: 0.855 ± 0.019
0.747TrpGlu: 0.747 ± 0.019
0.503TrpPhe: 0.503 ± 0.015
1.053TrpGly: 1.053 ± 0.023
0.355TrpHis: 0.355 ± 0.013
0.549TrpIle: 0.549 ± 0.015
0.367TrpLys: 0.367 ± 0.014
1.798TrpLeu: 1.798 ± 0.032
0.302TrpMet: 0.302 ± 0.013
0.412TrpAsn: 0.412 ± 0.015
0.808TrpPro: 0.808 ± 0.019
0.582TrpGln: 0.582 ± 0.017
1.367TrpArg: 1.367 ± 0.029
0.892TrpSer: 0.892 ± 0.02
1.044TrpThr: 1.044 ± 0.023
1.005TrpVal: 1.005 ± 0.023
0.352TrpTrp: 0.352 ± 0.015
0.369TrpTyr: 0.369 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.8TyrAla: 2.8 ± 0.038
0.176TyrCys: 0.176 ± 0.009
1.639TyrAsp: 1.639 ± 0.025
1.279TyrGlu: 1.279 ± 0.027
0.648TyrPhe: 0.648 ± 0.017
2.357TyrGly: 2.357 ± 0.04
0.388TyrHis: 0.388 ± 0.014
0.476TyrIle: 0.476 ± 0.015
0.392TyrLys: 0.392 ± 0.015
2.011TyrLeu: 2.011 ± 0.034
0.248TyrMet: 0.248 ± 0.011
0.397TyrAsn: 0.397 ± 0.015
1.041TyrPro: 1.041 ± 0.025
0.591TyrGln: 0.591 ± 0.016
1.848TyrArg: 1.848 ± 0.028
0.949TyrSer: 0.949 ± 0.024
1.195TyrThr: 1.195 ± 0.026
1.691TyrVal: 1.691 ± 0.027
0.334TyrTrp: 0.334 ± 0.012
0.448TyrTyr: 0.448 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6578 proteins (2140716 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski