Mirabilis leaf curl India virus associated betasatellite
Average proteome isoelectric point is 5.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A077V5F1|A0A077V5F1_9VIRU BetaC1 protein OS=Mirabilis leaf curl India virus associated betasatellite OX=1507403 GN=betaC1 PE=4 SV=1
MM1 pKa = 7.3 TIKK4 pKa = 10.58 YY5 pKa = 9.64 DD6 pKa = 3.62 NKK8 pKa = 9.82 RR9 pKa = 11.84 GMEE12 pKa = 3.79 FTVNVRR18 pKa = 11.84 LKK20 pKa = 9.88 NDD22 pKa = 2.91 NSIIVQIDD30 pKa = 3.76 LVSTRR35 pKa = 11.84 SPALAKK41 pKa = 10.55 AKK43 pKa = 10.15 FLIPYY48 pKa = 7.65 GHH50 pKa = 7.1 HH51 pKa = 6.83 GFTGPFDD58 pKa = 4.32 FNSLEE63 pKa = 3.77 EE64 pKa = 4.66 RR65 pKa = 11.84 ICHH68 pKa = 6.05 LLKK71 pKa = 9.63 ITYY74 pKa = 8.78 EE75 pKa = 4.09 DD76 pKa = 3.56 STIGEE81 pKa = 4.11 FRR83 pKa = 11.84 KK84 pKa = 10.26 EE85 pKa = 4.06 DD86 pKa = 3.5 MVEE89 pKa = 4.18 TIDD92 pKa = 4.19 ILMMHH97 pKa = 7.61 DD98 pKa = 4.23 APVFDD103 pKa = 4.83 INVHH107 pKa = 5.96 DD108 pKa = 5.39 LYY110 pKa = 11.29 DD111 pKa = 3.86 VYY113 pKa = 11.63 TNACVV118 pKa = 3.0
Molecular weight: 13.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.068
IPC2_protein 5.13
IPC_protein 5.041
Toseland 5.092
ProMoST 5.258
Dawson 5.118
Bjellqvist 5.219
Wikipedia 5.041
Rodwell 5.029
Grimsley 5.054
Solomon 5.118
Lehninger 5.08
Nozaki 5.27
DTASelect 5.461
Thurlkill 5.143
EMBOSS 5.143
Sillero 5.334
Patrickios 3.859
IPC_peptide 5.118
IPC2_peptide 5.334
IPC2.peptide.svr19 5.268
Protein with the highest isoelectric point:
>tr|A0A077V5F1|A0A077V5F1_9VIRU BetaC1 protein OS=Mirabilis leaf curl India virus associated betasatellite OX=1507403 GN=betaC1 PE=4 SV=1
MM1 pKa = 7.3 TIKK4 pKa = 10.58 YY5 pKa = 9.64 DD6 pKa = 3.62 NKK8 pKa = 9.82 RR9 pKa = 11.84 GMEE12 pKa = 3.79 FTVNVRR18 pKa = 11.84 LKK20 pKa = 9.88 NDD22 pKa = 2.91 NSIIVQIDD30 pKa = 3.76 LVSTRR35 pKa = 11.84 SPALAKK41 pKa = 10.55 AKK43 pKa = 10.15 FLIPYY48 pKa = 7.65 GHH50 pKa = 7.1 HH51 pKa = 6.83 GFTGPFDD58 pKa = 4.32 FNSLEE63 pKa = 3.77 EE64 pKa = 4.66 RR65 pKa = 11.84 ICHH68 pKa = 6.05 LLKK71 pKa = 9.63 ITYY74 pKa = 8.78 EE75 pKa = 4.09 DD76 pKa = 3.56 STIGEE81 pKa = 4.11 FRR83 pKa = 11.84 KK84 pKa = 10.26 EE85 pKa = 4.06 DD86 pKa = 3.5 MVEE89 pKa = 4.18 TIDD92 pKa = 4.19 ILMMHH97 pKa = 7.61 DD98 pKa = 4.23 APVFDD103 pKa = 4.83 INVHH107 pKa = 5.96 DD108 pKa = 5.39 LYY110 pKa = 11.29 DD111 pKa = 3.86 VYY113 pKa = 11.63 TNACVV118 pKa = 3.0
Molecular weight: 13.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.068
IPC2_protein 5.13
IPC_protein 5.041
Toseland 5.092
ProMoST 5.258
Dawson 5.118
Bjellqvist 5.219
Wikipedia 5.041
Rodwell 5.029
Grimsley 5.054
Solomon 5.118
Lehninger 5.08
Nozaki 5.27
DTASelect 5.461
Thurlkill 5.143
EMBOSS 5.143
Sillero 5.334
Patrickios 3.859
IPC_peptide 5.118
IPC2_peptide 5.334
IPC2.peptide.svr19 5.268
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
118
118
118
118.0
13.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.237 ± 0.0
1.695 ± 0.0
9.322 ± 0.0
5.932 ± 0.0
5.932 ± 0.0
4.237 ± 0.0
4.237 ± 0.0
9.322 ± 0.0
5.932 ± 0.0
7.627 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.237 ± 0.0
5.932 ± 0.0
3.39 ± 0.0
0.847 ± 0.0
4.237 ± 0.0
4.237 ± 0.0
6.78 ± 0.0
7.627 ± 0.0
0.0 ± 0.0
4.237 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here