Amino acid dipepetide frequency for Bacillus phage vB_BpsS-36

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.529AlaCys: 0.529 ± 0.201
3.303AlaAsp: 3.303 ± 0.455
4.69AlaGlu: 4.69 ± 0.543
2.841AlaPhe: 2.841 ± 0.484
6.012AlaGly: 6.012 ± 0.662
1.982AlaHis: 1.982 ± 0.368
4.096AlaIle: 4.096 ± 0.632
4.096AlaLys: 4.096 ± 0.54
6.144AlaLeu: 6.144 ± 0.599
1.85AlaMet: 1.85 ± 0.467
2.51AlaAsn: 2.51 ± 0.382
2.775AlaPro: 2.775 ± 0.461
2.709AlaGln: 2.709 ± 0.391
3.369AlaArg: 3.369 ± 0.475
3.501AlaSer: 3.501 ± 0.529
4.36AlaThr: 4.36 ± 0.64
5.549AlaVal: 5.549 ± 0.774
0.661AlaTrp: 0.661 ± 0.212
2.709AlaTyr: 2.709 ± 0.39
0.0AlaXaa: 0.0 ± 0.0
Cys
0.264CysAla: 0.264 ± 0.135
0.132CysCys: 0.132 ± 0.09
0.396CysAsp: 0.396 ± 0.148
0.529CysGlu: 0.529 ± 0.192
0.661CysPhe: 0.661 ± 0.187
0.991CysGly: 0.991 ± 0.384
0.198CysHis: 0.198 ± 0.11
0.33CysIle: 0.33 ± 0.187
1.255CysLys: 1.255 ± 0.338
0.859CysLeu: 0.859 ± 0.254
0.132CysMet: 0.132 ± 0.113
0.529CysAsn: 0.529 ± 0.21
0.264CysPro: 0.264 ± 0.144
0.529CysGln: 0.529 ± 0.162
0.33CysArg: 0.33 ± 0.174
0.33CysSer: 0.33 ± 0.207
0.595CysThr: 0.595 ± 0.221
0.198CysVal: 0.198 ± 0.109
0.0CysTrp: 0.0 ± 0.0
0.264CysTyr: 0.264 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
3.237AspAla: 3.237 ± 0.592
0.462AspCys: 0.462 ± 0.182
2.709AspAsp: 2.709 ± 0.473
4.69AspGlu: 4.69 ± 0.708
3.898AspPhe: 3.898 ± 0.457
3.832AspGly: 3.832 ± 0.583
1.255AspHis: 1.255 ± 0.367
4.36AspIle: 4.36 ± 0.614
5.748AspLys: 5.748 ± 0.732
4.69AspLeu: 4.69 ± 0.541
1.189AspMet: 1.189 ± 0.246
3.105AspAsn: 3.105 ± 0.53
2.048AspPro: 2.048 ± 0.343
1.586AspGln: 1.586 ± 0.324
2.841AspArg: 2.841 ± 0.533
3.435AspSer: 3.435 ± 0.435
3.7AspThr: 3.7 ± 0.721
3.7AspVal: 3.7 ± 0.407
1.057AspTrp: 1.057 ± 0.216
2.444AspTyr: 2.444 ± 0.328
0.0AspXaa: 0.0 ± 0.0
Glu
5.549GluAla: 5.549 ± 0.571
0.529GluCys: 0.529 ± 0.184
4.096GluAsp: 4.096 ± 0.534
8.39GluGlu: 8.39 ± 0.899
2.907GluPhe: 2.907 ± 0.444
6.012GluGly: 6.012 ± 0.732
1.982GluHis: 1.982 ± 0.402
4.558GluIle: 4.558 ± 0.435
6.738GluLys: 6.738 ± 0.8
10.57GluLeu: 10.57 ± 1.058
2.51GluMet: 2.51 ± 0.493
1.784GluAsn: 1.784 ± 0.43
1.85GluPro: 1.85 ± 0.477
2.841GluGln: 2.841 ± 0.502
4.294GluArg: 4.294 ± 0.515
4.492GluSer: 4.492 ± 0.517
4.03GluThr: 4.03 ± 0.492
6.805GluVal: 6.805 ± 0.766
1.255GluTrp: 1.255 ± 0.291
2.907GluTyr: 2.907 ± 0.435
0.0GluXaa: 0.0 ± 0.0
Phe
1.586PheAla: 1.586 ± 0.313
0.066PheCys: 0.066 ± 0.063
3.237PheAsp: 3.237 ± 0.489
3.237PheGlu: 3.237 ± 0.523
2.114PhePhe: 2.114 ± 0.444
2.841PheGly: 2.841 ± 0.405
1.189PheHis: 1.189 ± 0.347
2.378PheIle: 2.378 ± 0.424
3.039PheLys: 3.039 ± 0.407
3.039PheLeu: 3.039 ± 0.453
2.444PheMet: 2.444 ± 0.402
2.51PheAsn: 2.51 ± 0.32
1.387PhePro: 1.387 ± 0.25
1.85PheGln: 1.85 ± 0.337
1.519PheArg: 1.519 ± 0.249
2.576PheSer: 2.576 ± 0.375
4.096PheThr: 4.096 ± 0.5
1.784PheVal: 1.784 ± 0.353
0.33PheTrp: 0.33 ± 0.14
1.718PheTyr: 1.718 ± 0.343
0.0PheXaa: 0.0 ± 0.0
Gly
5.748GlyAla: 5.748 ± 0.69
0.661GlyCys: 0.661 ± 0.213
6.012GlyAsp: 6.012 ± 1.682
6.672GlyGlu: 6.672 ± 0.652
3.039GlyPhe: 3.039 ± 0.437
5.549GlyGly: 5.549 ± 0.581
1.718GlyHis: 1.718 ± 0.305
3.766GlyIle: 3.766 ± 0.465
4.955GlyLys: 4.955 ± 0.526
5.946GlyLeu: 5.946 ± 0.561
1.85GlyMet: 1.85 ± 0.4
3.633GlyAsn: 3.633 ± 0.636
5.153GlyPro: 5.153 ± 3.131
2.378GlyGln: 2.378 ± 0.412
2.973GlyArg: 2.973 ± 0.316
4.889GlySer: 4.889 ± 0.6
4.162GlyThr: 4.162 ± 0.642
5.417GlyVal: 5.417 ± 0.582
1.057GlyTrp: 1.057 ± 0.239
2.576GlyTyr: 2.576 ± 0.382
0.0GlyXaa: 0.0 ± 0.0
His
1.519HisAla: 1.519 ± 0.321
0.396HisCys: 0.396 ± 0.278
1.189HisAsp: 1.189 ± 0.342
1.652HisGlu: 1.652 ± 0.322
1.255HisPhe: 1.255 ± 0.297
2.18HisGly: 2.18 ± 0.375
0.462HisHis: 0.462 ± 0.195
0.925HisIle: 0.925 ± 0.23
0.991HisLys: 0.991 ± 0.274
1.519HisLeu: 1.519 ± 0.338
0.462HisMet: 0.462 ± 0.172
1.255HisAsn: 1.255 ± 0.295
0.859HisPro: 0.859 ± 0.204
0.661HisGln: 0.661 ± 0.209
0.925HisArg: 0.925 ± 0.218
1.453HisSer: 1.453 ± 0.315
0.793HisThr: 0.793 ± 0.214
1.255HisVal: 1.255 ± 0.263
0.396HisTrp: 0.396 ± 0.123
0.925HisTyr: 0.925 ± 0.246
0.0HisXaa: 0.0 ± 0.0
Ile
3.171IleAla: 3.171 ± 0.44
0.462IleCys: 0.462 ± 0.22
4.426IleAsp: 4.426 ± 0.683
5.615IleGlu: 5.615 ± 0.539
2.312IlePhe: 2.312 ± 0.451
3.766IleGly: 3.766 ± 0.617
0.793IleHis: 0.793 ± 0.284
2.709IleIle: 2.709 ± 0.43
5.285IleLys: 5.285 ± 0.737
3.567IleLeu: 3.567 ± 0.405
1.784IleMet: 1.784 ± 0.339
2.973IleAsn: 2.973 ± 0.418
1.982IlePro: 1.982 ± 0.303
2.18IleGln: 2.18 ± 0.29
2.907IleArg: 2.907 ± 0.531
2.246IleSer: 2.246 ± 0.444
3.832IleThr: 3.832 ± 0.521
3.435IleVal: 3.435 ± 0.348
0.462IleTrp: 0.462 ± 0.157
1.189IleTyr: 1.189 ± 0.227
0.0IleXaa: 0.0 ± 0.0
Lys
6.606LysAla: 6.606 ± 0.762
0.991LysCys: 0.991 ± 0.304
5.351LysAsp: 5.351 ± 0.781
7.531LysGlu: 7.531 ± 0.746
2.643LysPhe: 2.643 ± 0.527
5.946LysGly: 5.946 ± 1.259
1.586LysHis: 1.586 ± 0.326
4.294LysIle: 4.294 ± 0.668
7.795LysLys: 7.795 ± 1.015
7.267LysLeu: 7.267 ± 0.853
2.907LysMet: 2.907 ± 0.531
3.369LysAsn: 3.369 ± 0.496
2.51LysPro: 2.51 ± 0.517
2.841LysGln: 2.841 ± 0.465
4.228LysArg: 4.228 ± 0.507
3.435LysSer: 3.435 ± 0.589
3.039LysThr: 3.039 ± 0.453
5.483LysVal: 5.483 ± 0.731
0.529LysTrp: 0.529 ± 0.184
3.105LysTyr: 3.105 ± 0.605
0.0LysXaa: 0.0 ± 0.0
Leu
4.624LeuAla: 4.624 ± 0.673
0.727LeuCys: 0.727 ± 0.247
4.294LeuAsp: 4.294 ± 0.557
6.276LeuGlu: 6.276 ± 0.594
2.18LeuPhe: 2.18 ± 0.37
6.342LeuGly: 6.342 ± 0.603
2.114LeuHis: 2.114 ± 0.438
3.832LeuIle: 3.832 ± 0.545
7.531LeuLys: 7.531 ± 0.894
5.417LeuLeu: 5.417 ± 0.664
2.51LeuMet: 2.51 ± 0.415
4.096LeuAsn: 4.096 ± 0.522
2.643LeuPro: 2.643 ± 0.356
3.7LeuGln: 3.7 ± 0.531
3.898LeuArg: 3.898 ± 0.383
5.021LeuSer: 5.021 ± 0.589
5.417LeuThr: 5.417 ± 0.461
4.757LeuVal: 4.757 ± 0.64
0.793LeuTrp: 0.793 ± 0.278
2.775LeuTyr: 2.775 ± 0.397
0.0LeuXaa: 0.0 ± 0.0
Met
2.51MetAla: 2.51 ± 0.456
0.132MetCys: 0.132 ± 0.092
1.718MetAsp: 1.718 ± 0.339
2.973MetGlu: 2.973 ± 0.503
0.727MetPhe: 0.727 ± 0.263
1.916MetGly: 1.916 ± 0.354
0.198MetHis: 0.198 ± 0.12
2.246MetIle: 2.246 ± 0.386
2.841MetLys: 2.841 ± 0.429
1.652MetLeu: 1.652 ± 0.307
0.661MetMet: 0.661 ± 0.191
0.529MetAsn: 0.529 ± 0.213
0.727MetPro: 0.727 ± 0.199
0.793MetGln: 0.793 ± 0.268
1.982MetArg: 1.982 ± 0.384
2.444MetSer: 2.444 ± 0.34
1.784MetThr: 1.784 ± 0.433
2.114MetVal: 2.114 ± 0.427
0.0MetTrp: 0.0 ± 0.0
1.123MetTyr: 1.123 ± 0.282
0.0MetXaa: 0.0 ± 0.0
Asn
3.369AsnAla: 3.369 ± 0.49
0.529AsnCys: 0.529 ± 0.209
2.576AsnAsp: 2.576 ± 0.476
2.709AsnGlu: 2.709 ± 0.5
2.114AsnPhe: 2.114 ± 0.501
3.303AsnGly: 3.303 ± 0.495
0.991AsnHis: 0.991 ± 0.268
2.51AsnIle: 2.51 ± 0.384
3.766AsnLys: 3.766 ± 0.511
2.907AsnLeu: 2.907 ± 0.538
1.387AsnMet: 1.387 ± 0.298
3.237AsnAsn: 3.237 ± 0.559
1.255AsnPro: 1.255 ± 0.253
1.321AsnGln: 1.321 ± 0.338
2.18AsnArg: 2.18 ± 0.449
3.237AsnSer: 3.237 ± 0.599
2.444AsnThr: 2.444 ± 0.518
3.105AsnVal: 3.105 ± 0.526
1.057AsnTrp: 1.057 ± 0.221
1.387AsnTyr: 1.387 ± 0.307
0.0AsnXaa: 0.0 ± 0.0
Pro
3.039ProAla: 3.039 ± 0.413
0.264ProCys: 0.264 ± 0.123
1.916ProAsp: 1.916 ± 0.417
4.36ProGlu: 4.36 ± 0.641
1.982ProPhe: 1.982 ± 0.341
0.066ProGly: 0.066 ± 0.066
0.595ProHis: 0.595 ± 0.222
1.916ProIle: 1.916 ± 0.374
2.378ProLys: 2.378 ± 0.77
2.709ProLeu: 2.709 ± 0.512
0.793ProMet: 0.793 ± 0.234
0.925ProAsn: 0.925 ± 0.262
1.321ProPro: 1.321 ± 0.378
2.18ProGln: 2.18 ± 0.871
1.387ProArg: 1.387 ± 0.392
1.85ProSer: 1.85 ± 0.332
2.51ProThr: 2.51 ± 0.619
2.048ProVal: 2.048 ± 0.388
0.793ProTrp: 0.793 ± 0.273
1.255ProTyr: 1.255 ± 0.241
0.0ProXaa: 0.0 ± 0.0
Gln
3.039GlnAla: 3.039 ± 0.481
0.462GlnCys: 0.462 ± 0.183
1.519GlnAsp: 1.519 ± 0.268
3.435GlnGlu: 3.435 ± 0.509
1.453GlnPhe: 1.453 ± 0.266
4.69GlnGly: 4.69 ± 1.445
0.793GlnHis: 0.793 ± 0.23
1.916GlnIle: 1.916 ± 0.357
3.237GlnLys: 3.237 ± 0.496
3.435GlnLeu: 3.435 ± 0.415
0.595GlnMet: 0.595 ± 0.204
1.123GlnAsn: 1.123 ± 0.301
0.991GlnPro: 0.991 ± 0.276
1.057GlnGln: 1.057 ± 0.293
2.312GlnArg: 2.312 ± 0.405
1.255GlnSer: 1.255 ± 0.303
1.453GlnThr: 1.453 ± 0.322
2.246GlnVal: 2.246 ± 0.385
0.33GlnTrp: 0.33 ± 0.149
0.925GlnTyr: 0.925 ± 0.292
0.0GlnXaa: 0.0 ± 0.0
Arg
4.294ArgAla: 4.294 ± 0.504
0.529ArgCys: 0.529 ± 0.193
2.973ArgAsp: 2.973 ± 0.499
3.832ArgGlu: 3.832 ± 0.513
2.048ArgPhe: 2.048 ± 0.361
3.766ArgGly: 3.766 ± 0.699
0.462ArgHis: 0.462 ± 0.215
2.576ArgIle: 2.576 ± 0.409
4.624ArgLys: 4.624 ± 0.546
3.039ArgLeu: 3.039 ± 0.59
1.453ArgMet: 1.453 ± 0.384
2.312ArgAsn: 2.312 ± 0.343
0.859ArgPro: 0.859 ± 0.203
1.982ArgGln: 1.982 ± 0.391
2.841ArgArg: 2.841 ± 0.579
2.114ArgSer: 2.114 ± 0.438
2.444ArgThr: 2.444 ± 0.357
4.162ArgVal: 4.162 ± 0.564
0.595ArgTrp: 0.595 ± 0.233
2.444ArgTyr: 2.444 ± 0.386
0.0ArgXaa: 0.0 ± 0.0
Ser
2.907SerAla: 2.907 ± 0.404
0.198SerCys: 0.198 ± 0.132
2.775SerAsp: 2.775 ± 0.443
3.501SerGlu: 3.501 ± 0.491
3.633SerPhe: 3.633 ± 0.444
4.955SerGly: 4.955 ± 0.622
1.189SerHis: 1.189 ± 0.339
2.841SerIle: 2.841 ± 0.499
4.228SerLys: 4.228 ± 0.591
3.898SerLeu: 3.898 ± 0.703
1.916SerMet: 1.916 ± 0.383
3.369SerAsn: 3.369 ± 0.667
2.114SerPro: 2.114 ± 0.357
1.916SerGln: 1.916 ± 0.338
3.039SerArg: 3.039 ± 0.4
4.823SerSer: 4.823 ± 0.796
3.369SerThr: 3.369 ± 0.564
3.237SerVal: 3.237 ± 0.412
0.991SerTrp: 0.991 ± 0.294
1.916SerTyr: 1.916 ± 0.373
0.0SerXaa: 0.0 ± 0.0
Thr
4.558ThrAla: 4.558 ± 0.503
0.33ThrCys: 0.33 ± 0.175
3.501ThrAsp: 3.501 ± 0.556
4.492ThrGlu: 4.492 ± 0.599
2.378ThrPhe: 2.378 ± 0.477
7.531ThrGly: 7.531 ± 1.86
1.123ThrHis: 1.123 ± 0.287
3.898ThrIle: 3.898 ± 0.451
2.973ThrLys: 2.973 ± 0.429
3.898ThrLeu: 3.898 ± 0.424
1.255ThrMet: 1.255 ± 0.328
2.378ThrAsn: 2.378 ± 0.538
3.105ThrPro: 3.105 ± 0.698
1.586ThrGln: 1.586 ± 0.344
1.85ThrArg: 1.85 ± 0.311
2.775ThrSer: 2.775 ± 0.557
2.775ThrThr: 2.775 ± 0.48
4.823ThrVal: 4.823 ± 0.62
0.859ThrTrp: 0.859 ± 0.219
2.18ThrTyr: 2.18 ± 0.456
0.0ThrXaa: 0.0 ± 0.0
Val
5.021ValAla: 5.021 ± 0.723
0.529ValCys: 0.529 ± 0.227
4.69ValAsp: 4.69 ± 0.666
4.823ValGlu: 4.823 ± 0.569
2.576ValPhe: 2.576 ± 0.465
4.558ValGly: 4.558 ± 0.659
1.387ValHis: 1.387 ± 0.332
3.567ValIle: 3.567 ± 0.501
6.078ValLys: 6.078 ± 0.678
4.889ValLeu: 4.889 ± 0.623
1.916ValMet: 1.916 ± 0.404
2.907ValAsn: 2.907 ± 0.429
1.718ValPro: 1.718 ± 0.397
2.378ValGln: 2.378 ± 0.403
3.832ValArg: 3.832 ± 0.633
3.964ValSer: 3.964 ± 0.501
4.624ValThr: 4.624 ± 0.576
3.766ValVal: 3.766 ± 0.623
0.793ValTrp: 0.793 ± 0.207
2.973ValTyr: 2.973 ± 0.447
0.0ValXaa: 0.0 ± 0.0
Trp
0.198TrpAla: 0.198 ± 0.092
0.462TrpCys: 0.462 ± 0.157
0.727TrpAsp: 0.727 ± 0.166
1.057TrpGlu: 1.057 ± 0.302
0.462TrpPhe: 0.462 ± 0.171
0.727TrpGly: 0.727 ± 0.222
0.198TrpHis: 0.198 ± 0.108
0.595TrpIle: 0.595 ± 0.209
0.529TrpLys: 0.529 ± 0.169
0.859TrpLeu: 0.859 ± 0.218
0.595TrpMet: 0.595 ± 0.191
0.727TrpAsn: 0.727 ± 0.196
0.0TrpPro: 0.0 ± 0.0
0.396TrpGln: 0.396 ± 0.165
0.727TrpArg: 0.727 ± 0.172
1.453TrpSer: 1.453 ± 0.456
0.991TrpThr: 0.991 ± 0.277
0.859TrpVal: 0.859 ± 0.253
0.198TrpTrp: 0.198 ± 0.111
0.661TrpTyr: 0.661 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.444TyrAla: 2.444 ± 0.37
0.396TyrCys: 0.396 ± 0.179
2.576TyrAsp: 2.576 ± 0.43
3.633TyrGlu: 3.633 ± 0.495
1.718TyrPhe: 1.718 ± 0.369
3.171TyrGly: 3.171 ± 0.368
0.859TyrHis: 0.859 ± 0.245
1.784TyrIle: 1.784 ± 0.407
3.369TyrLys: 3.369 ± 0.477
2.643TyrLeu: 2.643 ± 0.471
0.793TyrMet: 0.793 ± 0.255
2.114TyrAsn: 2.114 ± 0.386
0.727TyrPro: 0.727 ± 0.227
1.387TyrGln: 1.387 ± 0.349
1.916TyrArg: 1.916 ± 0.377
1.519TyrSer: 1.519 ± 0.302
1.85TyrThr: 1.85 ± 0.342
2.246TyrVal: 2.246 ± 0.356
0.264TyrTrp: 0.264 ± 0.127
1.519TyrTyr: 1.519 ± 0.335
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (15138 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski