Streptococcus phage Javan180

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ATL6|A0A4D6ATL6_9CAUD Hyaluronate lyase OS=Streptococcus phage Javan180 OX=2548028 GN=Javan180_0011 PE=4 SV=1
MM1 pKa = 7.14LTYY4 pKa = 10.82EE5 pKa = 4.4EE6 pKa = 4.41FKK8 pKa = 10.83QAIDD12 pKa = 3.12HH13 pKa = 6.57GYY15 pKa = 7.61ITGDD19 pKa = 2.69VVMIVRR25 pKa = 11.84KK26 pKa = 9.89NGQIFDD32 pKa = 3.85YY33 pKa = 10.12VLPGEE38 pKa = 4.72EE39 pKa = 4.07VQPWEE44 pKa = 4.36VVTVEE49 pKa = 4.15AVADD53 pKa = 3.75ILNEE57 pKa = 3.87LQIII61 pKa = 4.15

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AS62|A0A4D6AS62_9CAUD Uncharacterized protein OS=Streptococcus phage Javan180 OX=2548028 GN=Javan180_0022 PE=4 SV=1
MM1 pKa = 7.51ILFVADD7 pKa = 3.68ISLKK11 pKa = 10.48VVGTTGLKK19 pKa = 10.5NKK21 pKa = 10.46LEE23 pKa = 4.81LIAKK27 pKa = 9.6KK28 pKa = 10.02EE29 pKa = 3.91AVKK32 pKa = 10.7KK33 pKa = 10.18IVRR36 pKa = 11.84DD37 pKa = 3.52NGKK40 pKa = 8.71QLHH43 pKa = 6.18RR44 pKa = 11.84KK45 pKa = 7.38MVQKK49 pKa = 10.82AVFTKK54 pKa = 10.28GHH56 pKa = 5.44STGATRR62 pKa = 11.84ISITMQIGDD71 pKa = 4.58DD72 pKa = 4.02GFSVTVKK79 pKa = 10.49PGTHH83 pKa = 5.03YY84 pKa = 10.93AGYY87 pKa = 9.82LEE89 pKa = 4.58KK90 pKa = 10.31GTRR93 pKa = 11.84FMSKK97 pKa = 9.79QPFVLPALKK106 pKa = 9.62EE107 pKa = 4.0QKK109 pKa = 10.45VKK111 pKa = 10.34FRR113 pKa = 11.84KK114 pKa = 10.07DD115 pKa = 2.98LEE117 pKa = 4.36ALVKK121 pKa = 10.71

Molecular weight: 13.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

9550

59

1084

258.1

29.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.529 ± 0.934

0.492 ± 0.093

6.827 ± 0.443

7.047 ± 0.633

3.916 ± 0.225

6.565 ± 0.625

1.455 ± 0.174

6.921 ± 0.257

9.288 ± 0.566

8.44 ± 0.337

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.325 ± 0.164

4.827 ± 0.316

2.408 ± 0.283

4.241 ± 0.294

4.304 ± 0.295

6.565 ± 0.568

5.916 ± 0.291

6.209 ± 0.324

0.775 ± 0.126

3.948 ± 0.419
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski