Streptococcus pasteurianus (strain ATCC 43144 / JCM 5346 / CDC 1723-81)
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1864 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F5X2E7|F5X2E7_STRPX Predicted membrane protein OS=Streptococcus pasteurianus (strain ATCC 43144 / JCM 5346 / CDC 1723-81) OX=981540 GN=SGPB_1420 PE=4 SV=1
MM1 pKa = 7.42 PTRR4 pKa = 11.84 NYY6 pKa = 10.48 SRR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.05 RR11 pKa = 11.84 QQKK14 pKa = 8.77 SQIIALTGLVTAAVALVLILAKK36 pKa = 10.67 LIFSGVTTLASDD48 pKa = 4.29 GSLDD52 pKa = 4.09 LSDD55 pKa = 4.43 ANSYY59 pKa = 10.42 DD60 pKa = 3.26 VSEE63 pKa = 4.44 IATVSTSDD71 pKa = 3.87 AIIYY75 pKa = 9.48 TDD77 pKa = 4.74 DD78 pKa = 3.99 GSSSKK83 pKa = 10.35 ISEE86 pKa = 4.03 NTAITISAYY95 pKa = 10.81 YY96 pKa = 9.11 YY97 pKa = 10.69 DD98 pKa = 5.47 AMLNDD103 pKa = 4.26 EE104 pKa = 5.05 DD105 pKa = 3.96 TTLAQFSMNGDD116 pKa = 3.53 TYY118 pKa = 11.5 YY119 pKa = 10.33 IDD121 pKa = 3.68 TNDD124 pKa = 3.95 ISLEE128 pKa = 3.79 QDD130 pKa = 3.04 NTINAYY136 pKa = 9.26 IAEE139 pKa = 4.32 TLGYY143 pKa = 9.41 SHH145 pKa = 7.5 ADD147 pKa = 2.73 ITDD150 pKa = 4.54 DD151 pKa = 3.25 IKK153 pKa = 11.62 SSFEE157 pKa = 3.4 QAAYY161 pKa = 8.35 KK162 pKa = 10.15 TDD164 pKa = 3.57 DD165 pKa = 3.89 GKK167 pKa = 10.1 PLGVIIHH174 pKa = 6.48 DD175 pKa = 3.8 TGVDD179 pKa = 3.13 NSTIEE184 pKa = 4.3 SEE186 pKa = 4.38 VNYY189 pKa = 9.55 MVQNYY194 pKa = 9.52 DD195 pKa = 3.19 KK196 pKa = 10.66 EE197 pKa = 4.78 GVFVHH202 pKa = 6.45 SFIDD206 pKa = 3.05 SDD208 pKa = 4.26 TILRR212 pKa = 11.84 IADD215 pKa = 3.7 EE216 pKa = 4.95 GYY218 pKa = 9.73 KK219 pKa = 10.59 AQGAGANANPYY230 pKa = 9.49 YY231 pKa = 10.18 IQFEE235 pKa = 4.5 LTHH238 pKa = 6.78 EE239 pKa = 4.54 DD240 pKa = 3.54 SQKK243 pKa = 11.04 GFAEE247 pKa = 3.95 QLANAAYY254 pKa = 7.84 YY255 pKa = 7.92 TAYY258 pKa = 9.85 MLKK261 pKa = 10.29 KK262 pKa = 9.59 YY263 pKa = 10.0 DD264 pKa = 3.8 LPVTLGQEE272 pKa = 4.26 DD273 pKa = 4.81 GEE275 pKa = 4.67 GTIWTHH281 pKa = 5.77 EE282 pKa = 4.24 MVSLYY287 pKa = 10.84 LGGTDD292 pKa = 4.6 HH293 pKa = 7.64 VDD295 pKa = 3.12 PTDD298 pKa = 3.34 YY299 pKa = 8.5 WTEE302 pKa = 3.73 TANDD306 pKa = 3.92 YY307 pKa = 11.19 FGTDD311 pKa = 3.11 YY312 pKa = 11.1 DD313 pKa = 3.69 IEE315 pKa = 4.76 DD316 pKa = 3.9 FVEE319 pKa = 4.96 LVQAYY324 pKa = 10.1 YY325 pKa = 10.88 NALL328 pKa = 3.56
Molecular weight: 36.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.816
IPC2_protein 3.897
IPC_protein 3.91
Toseland 3.694
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.846
Rodwell 3.745
Grimsley 3.605
Solomon 3.897
Lehninger 3.859
Nozaki 4.012
DTASelect 4.266
Thurlkill 3.745
EMBOSS 3.846
Sillero 4.037
Patrickios 0.477
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.918
Protein with the highest isoelectric point:
>tr|F5X3R9|F5X3R9_STRPX 30S ribosomal protein S2 OS=Streptococcus pasteurianus (strain ATCC 43144 / JCM 5346 / CDC 1723-81) OX=981540 GN=rpsB PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1864
0
1864
569143
38
1998
305.3
34.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.662 ± 0.056
0.568 ± 0.013
5.954 ± 0.048
6.81 ± 0.057
4.604 ± 0.043
6.488 ± 0.056
1.876 ± 0.023
7.596 ± 0.051
6.717 ± 0.046
9.871 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.026
4.719 ± 0.039
3.138 ± 0.029
3.743 ± 0.036
3.819 ± 0.042
6.204 ± 0.051
5.914 ± 0.047
7.106 ± 0.05
0.857 ± 0.018
3.802 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here