Amino acid dipepetide frequency for Clostridium sp. CAG:1024

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.493AlaAla: 13.493 ± 0.251	1.773AlaCys: 1.773 ± 0.06	5.757AlaAsp: 5.757 ± 0.116	7.718AlaGlu: 7.718 ± 0.146	4.02AlaPhe: 4.02 ± 0.09	8.095AlaGly: 8.095 ± 0.138	1.759AlaHis: 1.759 ± 0.063	5.506AlaIle: 5.506 ± 0.104	4.612AlaLys: 4.612 ± 0.098	11.607AlaLeu: 11.607 ± 0.166	3.028AlaMet: 3.028 ± 0.073	2.605AlaAsn: 2.605 ± 0.068	3.487AlaPro: 3.487 ± 0.085	2.858AlaGln: 2.858 ± 0.075	5.406AlaArg: 5.406 ± 0.108	4.97AlaSer: 4.97 ± 0.113	4.12AlaThr: 4.12 ± 0.106	8.248AlaVal: 8.248 ± 0.148	0.859AlaTrp: 0.859 ± 0.039	3.423AlaTyr: 3.423 ± 0.088	0.005AlaXaa: 0.005 ± 0.003
Cys	1.706CysAla: 1.706 ± 0.059	0.49CysCys: 0.49 ± 0.035	0.93CysAsp: 0.93 ± 0.042	0.858CysGlu: 0.858 ± 0.04	0.748CysPhe: 0.748 ± 0.034	1.886CysGly: 1.886 ± 0.058	0.33CysHis: 0.33 ± 0.022	1.096CysIle: 1.096 ± 0.045	0.77CysLys: 0.77 ± 0.04	1.344CysLeu: 1.344 ± 0.054	0.464CysMet: 0.464 ± 0.033	0.508CysAsn: 0.508 ± 0.03	0.725CysPro: 0.725 ± 0.04	0.306CysGln: 0.306 ± 0.025	1.118CysArg: 1.118 ± 0.053	0.996CysSer: 0.996 ± 0.042	1.04CysThr: 1.04 ± 0.044	1.225CysVal: 1.225 ± 0.056	0.146CysTrp: 0.146 ± 0.015	0.592CysTyr: 0.592 ± 0.034	0.0CysXaa: 0.0 ± 0.0
Asp	6.784AspAla: 6.784 ± 0.134	1.069AspCys: 1.069 ± 0.049	2.736AspAsp: 2.736 ± 0.083	4.098AspGlu: 4.098 ± 0.101	2.121AspPhe: 2.121 ± 0.059	5.256AspGly: 5.256 ± 0.117	0.876AspHis: 0.876 ± 0.048	3.164AspIle: 3.164 ± 0.091	2.132AspLys: 2.132 ± 0.078	4.555AspLeu: 4.555 ± 0.091	1.664AspMet: 1.664 ± 0.05	1.486AspAsn: 1.486 ± 0.053	2.438AspPro: 2.438 ± 0.068	1.076AspGln: 1.076 ± 0.045	3.35AspArg: 3.35 ± 0.077	2.358AspSer: 2.358 ± 0.072	3.031AspThr: 3.031 ± 0.071	4.186AspVal: 4.186 ± 0.094	0.588AspTrp: 0.588 ± 0.031	2.057AspTyr: 2.057 ± 0.07	0.002AspXaa: 0.002 ± 0.002
Glu	6.389GluAla: 6.389 ± 0.126	0.792GluCys: 0.792 ± 0.04	3.232GluAsp: 3.232 ± 0.089	4.57GluGlu: 4.57 ± 0.117	1.833GluPhe: 1.833 ± 0.057	4.067GluGly: 4.067 ± 0.09	1.369GluHis: 1.369 ± 0.051	4.215GluIle: 4.215 ± 0.112	4.075GluLys: 4.075 ± 0.097	6.39GluLeu: 6.39 ± 0.128	2.039GluMet: 2.039 ± 0.059	2.962GluAsn: 2.962 ± 0.075	2.445GluPro: 2.445 ± 0.083	3.104GluGln: 3.104 ± 0.093	5.284GluArg: 5.284 ± 0.126	3.077GluSer: 3.077 ± 0.08	4.532GluThr: 4.532 ± 0.109	3.621GluVal: 3.621 ± 0.086	0.53GluTrp: 0.53 ± 0.032	2.096GluTyr: 2.096 ± 0.066	0.0GluXaa: 0.0 ± 0.0
Phe	3.725PheAla: 3.725 ± 0.091	0.819PheCys: 0.819 ± 0.042	2.664PheAsp: 2.664 ± 0.073	2.179PheGlu: 2.179 ± 0.076	1.653PhePhe: 1.653 ± 0.059	3.248PheGly: 3.248 ± 0.087	0.732PheHis: 0.732 ± 0.035	1.806PheIle: 1.806 ± 0.078	1.147PheLys: 1.147 ± 0.053	3.936PheLeu: 3.936 ± 0.115	0.821PheMet: 0.821 ± 0.04	1.162PheAsn: 1.162 ± 0.053	1.566PhePro: 1.566 ± 0.054	1.067PheGln: 1.067 ± 0.048	2.383PheArg: 2.383 ± 0.072	2.935PheSer: 2.935 ± 0.079	2.134PheThr: 2.134 ± 0.076	2.864PheVal: 2.864 ± 0.077	0.302PheTrp: 0.302 ± 0.023	1.222PheTyr: 1.222 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	7.213GlyAla: 7.213 ± 0.133	1.466GlyCys: 1.466 ± 0.064	3.927GlyAsp: 3.927 ± 0.085	5.071GlyGlu: 5.071 ± 0.105	3.033GlyPhe: 3.033 ± 0.077	5.932GlyGly: 5.932 ± 0.13	1.378GlyHis: 1.378 ± 0.055	4.898GlyIle: 4.898 ± 0.114	4.675GlyLys: 4.675 ± 0.09	7.166GlyLeu: 7.166 ± 0.124	2.66GlyMet: 2.66 ± 0.073	2.514GlyAsn: 2.514 ± 0.082	1.511GlyPro: 1.511 ± 0.058	2.096GlyGln: 2.096 ± 0.07	4.273GlyArg: 4.273 ± 0.111	4.259GlySer: 4.259 ± 0.097	4.634GlyThr: 4.634 ± 0.109	6.281GlyVal: 6.281 ± 0.137	0.732GlyTrp: 0.732 ± 0.036	3.017GlyTyr: 3.017 ± 0.075	0.005GlyXaa: 0.005 ± 0.003
His	1.946HisAla: 1.946 ± 0.063	0.384HisCys: 0.384 ± 0.028	1.143HisAsp: 1.143 ± 0.054	1.152HisGlu: 1.152 ± 0.049	0.726HisPhe: 0.726 ± 0.033	1.821HisGly: 1.821 ± 0.077	0.382HisHis: 0.382 ± 0.027	1.316HisIle: 1.316 ± 0.052	0.697HisLys: 0.697 ± 0.032	1.624HisLeu: 1.624 ± 0.068	0.45HisMet: 0.45 ± 0.034	0.604HisAsn: 0.604 ± 0.032	1.029HisPro: 1.029 ± 0.041	0.373HisGln: 0.373 ± 0.024	1.076HisArg: 1.076 ± 0.048	0.943HisSer: 0.943 ± 0.043	1.087HisThr: 1.087 ± 0.047	1.36HisVal: 1.36 ± 0.057	0.16HisTrp: 0.16 ± 0.019	0.555HisTyr: 0.555 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.54IleAla: 6.54 ± 0.119	1.08IleCys: 1.08 ± 0.041	3.556IleAsp: 3.556 ± 0.087	3.891IleGlu: 3.891 ± 0.095	1.755IlePhe: 1.755 ± 0.055	4.905IleGly: 4.905 ± 0.098	0.996IleHis: 0.996 ± 0.049	2.951IleIle: 2.951 ± 0.09	2.106IleLys: 2.106 ± 0.07	5.781IleLeu: 5.781 ± 0.118	1.236IleMet: 1.236 ± 0.048	1.733IleAsn: 1.733 ± 0.061	2.771IlePro: 2.771 ± 0.072	1.486IleGln: 1.486 ± 0.054	4.04IleArg: 4.04 ± 0.095	3.339IleSer: 3.339 ± 0.081	3.026IleThr: 3.026 ± 0.084	4.936IleVal: 4.936 ± 0.1	0.477IleTrp: 0.477 ± 0.031	1.568IleTyr: 1.568 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	4.28LysAla: 4.28 ± 0.095	0.51LysCys: 0.51 ± 0.027	2.259LysAsp: 2.259 ± 0.07	3.199LysGlu: 3.199 ± 0.084	1.005LysPhe: 1.005 ± 0.046	2.742LysGly: 2.742 ± 0.062	0.89LysHis: 0.89 ± 0.04	2.5LysIle: 2.5 ± 0.082	3.192LysLys: 3.192 ± 0.095	4.482LysLeu: 4.482 ± 0.103	1.294LysMet: 1.294 ± 0.061	2.121LysAsn: 2.121 ± 0.064	1.99LysPro: 1.99 ± 0.067	1.813LysGln: 1.813 ± 0.057	3.687LysArg: 3.687 ± 0.08	2.37LysSer: 2.37 ± 0.07	3.232LysThr: 3.232 ± 0.084	2.536LysVal: 2.536 ± 0.082	0.399LysTrp: 0.399 ± 0.03	1.517LysTyr: 1.517 ± 0.053	0.0LysXaa: 0.0 ± 0.0
Leu	9.706LeuAla: 9.706 ± 0.152	2.017LeuCys: 2.017 ± 0.058	5.398LeuAsp: 5.398 ± 0.106	5.433LeuGlu: 5.433 ± 0.112	4.539LeuPhe: 4.539 ± 0.108	6.802LeuGly: 6.802 ± 0.135	2.236LeuHis: 2.236 ± 0.066	5.491LeuIle: 5.491 ± 0.122	4.14LeuLys: 4.14 ± 0.092	12.16LeuLeu: 12.16 ± 0.264	2.553LeuMet: 2.553 ± 0.079	2.891LeuAsn: 2.891 ± 0.068	4.941LeuPro: 4.941 ± 0.101	2.831LeuGln: 2.831 ± 0.076	6.542LeuArg: 6.542 ± 0.138	7.175LeuSer: 7.175 ± 0.126	5.761LeuThr: 5.761 ± 0.104	6.203LeuVal: 6.203 ± 0.12	0.81LeuTrp: 0.81 ± 0.043	3.525LeuTyr: 3.525 ± 0.086	0.0LeuXaa: 0.0 ± 0.0
Met	2.5MetAla: 2.5 ± 0.074	0.317MetCys: 0.317 ± 0.028	1.493MetAsp: 1.493 ± 0.053	2.076MetGlu: 2.076 ± 0.07	0.876MetPhe: 0.876 ± 0.042	1.802MetGly: 1.802 ± 0.055	0.543MetHis: 0.543 ± 0.031	1.491MetIle: 1.491 ± 0.05	1.637MetLys: 1.637 ± 0.057	3.05MetLeu: 3.05 ± 0.08	0.675MetMet: 0.675 ± 0.036	1.049MetAsn: 1.049 ± 0.044	1.3MetPro: 1.3 ± 0.047	1.4MetGln: 1.4 ± 0.047	1.985MetArg: 1.985 ± 0.061	1.416MetSer: 1.416 ± 0.045	1.568MetThr: 1.568 ± 0.055	1.524MetVal: 1.524 ± 0.053	0.197MetTrp: 0.197 ± 0.021	0.772MetTyr: 0.772 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	3.953AsnAla: 3.953 ± 0.091	0.574AsnCys: 0.574 ± 0.034	1.733AsnAsp: 1.733 ± 0.061	1.957AsnGlu: 1.957 ± 0.065	0.978AsnPhe: 0.978 ± 0.043	3.368AsnGly: 3.368 ± 0.094	0.586AsnHis: 0.586 ± 0.035	2.008AsnIle: 2.008 ± 0.072	1.196AsnLys: 1.196 ± 0.05	2.733AsnLeu: 2.733 ± 0.075	0.945AsnMet: 0.945 ± 0.04	1.032AsnAsn: 1.032 ± 0.049	1.657AsnPro: 1.657 ± 0.055	0.686AsnGln: 0.686 ± 0.04	1.79AsnArg: 1.79 ± 0.068	1.462AsnSer: 1.462 ± 0.058	1.768AsnThr: 1.768 ± 0.06	2.345AsnVal: 2.345 ± 0.073	0.313AsnTrp: 0.313 ± 0.023	1.143AsnTyr: 1.143 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	4.399ProAla: 4.399 ± 0.104	0.619ProCys: 0.619 ± 0.034	2.698ProAsp: 2.698 ± 0.068	3.599ProGlu: 3.599 ± 0.09	1.751ProPhe: 1.751 ± 0.058	2.858ProGly: 2.858 ± 0.076	0.785ProHis: 0.785 ± 0.039	2.154ProIle: 2.154 ± 0.072	1.753ProLys: 1.753 ± 0.057	3.745ProLeu: 3.745 ± 0.085	1.067ProMet: 1.067 ± 0.044	1.285ProAsn: 1.285 ± 0.05	1.287ProPro: 1.287 ± 0.057	1.145ProGln: 1.145 ± 0.048	1.75ProArg: 1.75 ± 0.058	2.15ProSer: 2.15 ± 0.069	2.208ProThr: 2.208 ± 0.085	3.514ProVal: 3.514 ± 0.067	0.344ProTrp: 0.344 ± 0.025	1.433ProTyr: 1.433 ± 0.055	0.009ProXaa: 0.009 ± 0.004
Gln	2.698GlnAla: 2.698 ± 0.081	0.373GlnCys: 0.373 ± 0.025	1.38GlnAsp: 1.38 ± 0.051	1.753GlnGlu: 1.753 ± 0.055	0.959GlnPhe: 0.959 ± 0.04	1.792GlnGly: 1.792 ± 0.061	0.645GlnHis: 0.645 ± 0.037	1.83GlnIle: 1.83 ± 0.055	1.943GlnLys: 1.943 ± 0.058	2.864GlnLeu: 2.864 ± 0.081	0.959GlnMet: 0.959 ± 0.047	1.249GlnAsn: 1.249 ± 0.044	1.178GlnPro: 1.178 ± 0.05	1.395GlnGln: 1.395 ± 0.064	2.341GlnArg: 2.341 ± 0.077	1.713GlnSer: 1.713 ± 0.054	1.728GlnThr: 1.728 ± 0.053	1.562GlnVal: 1.562 ± 0.051	0.268GlnTrp: 0.268 ± 0.022	1.174GlnTyr: 1.174 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	5.808ArgAla: 5.808 ± 0.119	1.078ArgCys: 1.078 ± 0.045	3.323ArgAsp: 3.323 ± 0.091	4.657ArgGlu: 4.657 ± 0.117	2.871ArgPhe: 2.871 ± 0.08	3.962ArgGly: 3.962 ± 0.111	1.273ArgHis: 1.273 ± 0.058	4.675ArgIle: 4.675 ± 0.101	3.03ArgLys: 3.03 ± 0.091	6.218ArgLeu: 6.218 ± 0.144	1.946ArgMet: 1.946 ± 0.062	1.964ArgAsn: 1.964 ± 0.063	2.314ArgPro: 2.314 ± 0.072	2.074ArgGln: 2.074 ± 0.056	4.697ArgArg: 4.697 ± 0.144	3.239ArgSer: 3.239 ± 0.073	3.172ArgThr: 3.172 ± 0.079	4.049ArgVal: 4.049 ± 0.105	0.472ArgTrp: 0.472 ± 0.029	2.29ArgTyr: 2.29 ± 0.064	0.004ArgXaa: 0.004 ± 0.002
Ser	5.842SerAla: 5.842 ± 0.116	0.887SerCys: 0.887 ± 0.039	3.359SerAsp: 3.359 ± 0.091	3.643SerGlu: 3.643 ± 0.093	2.4SerPhe: 2.4 ± 0.075	5.318SerGly: 5.318 ± 0.1	0.965SerHis: 0.965 ± 0.047	3.323SerIle: 3.323 ± 0.068	2.088SerLys: 2.088 ± 0.058	5.211SerLeu: 5.211 ± 0.104	1.529SerMet: 1.529 ± 0.048	1.589SerAsn: 1.589 ± 0.056	2.045SerPro: 2.045 ± 0.072	1.167SerGln: 1.167 ± 0.047	3.033SerArg: 3.033 ± 0.077	2.975SerSer: 2.975 ± 0.087	2.827SerThr: 2.827 ± 0.077	4.446SerVal: 4.446 ± 0.095	0.426SerTrp: 0.426 ± 0.025	1.868SerTyr: 1.868 ± 0.061	0.002SerXaa: 0.002 ± 0.002
Thr	6.063ThrAla: 6.063 ± 0.129	0.692ThrCys: 0.692 ± 0.033	3.082ThrAsp: 3.082 ± 0.077	3.84ThrGlu: 3.84 ± 0.097	2.025ThrPhe: 2.025 ± 0.067	4.71ThrGly: 4.71 ± 0.1	1.029ThrHis: 1.029 ± 0.04	3.243ThrIle: 3.243 ± 0.092	2.116ThrLys: 2.116 ± 0.068	6.128ThrLeu: 6.128 ± 0.106	1.38ThrMet: 1.38 ± 0.054	1.522ThrAsn: 1.522 ± 0.058	3.033ThrPro: 3.033 ± 0.095	1.344ThrGln: 1.344 ± 0.05	2.689ThrArg: 2.689 ± 0.061	2.543ThrSer: 2.543 ± 0.071	2.733ThrThr: 2.733 ± 0.094	5.205ThrVal: 5.205 ± 0.156	0.364ThrTrp: 0.364 ± 0.029	1.741ThrTyr: 1.741 ± 0.07	0.002ThrXaa: 0.002 ± 0.002
Val	6.112ValAla: 6.112 ± 0.122	1.513ValCys: 1.513 ± 0.062	3.72ValAsp: 3.72 ± 0.087	4.335ValGlu: 4.335 ± 0.087	3.325ValPhe: 3.325 ± 0.09	4.903ValGly: 4.903 ± 0.104	1.278ValHis: 1.278 ± 0.048	4.044ValIle: 4.044 ± 0.101	3.088ValLys: 3.088 ± 0.083	7.909ValLeu: 7.909 ± 0.125	1.812ValMet: 1.812 ± 0.059	2.27ValAsn: 2.27 ± 0.068	3.175ValPro: 3.175 ± 0.084	2.234ValGln: 2.234 ± 0.062	4.756ValArg: 4.756 ± 0.115	4.737ValSer: 4.737 ± 0.104	4.237ValThr: 4.237 ± 0.115	5.043ValVal: 5.043 ± 0.12	0.614ValTrp: 0.614 ± 0.037	2.675ValTyr: 2.675 ± 0.071	0.005ValXaa: 0.005 ± 0.003
Trp	0.615TrpAla: 0.615 ± 0.034	0.166TrpCys: 0.166 ± 0.019	0.466TrpAsp: 0.466 ± 0.029	0.462TrpGlu: 0.462 ± 0.026	0.348TrpPhe: 0.348 ± 0.027	0.546TrpGly: 0.546 ± 0.036	0.151TrpHis: 0.151 ± 0.015	0.581TrpIle: 0.581 ± 0.031	0.477TrpLys: 0.477 ± 0.034	0.843TrpLeu: 0.843 ± 0.037	0.289TrpMet: 0.289 ± 0.025	0.45TrpAsn: 0.45 ± 0.032	0.275TrpPro: 0.275 ± 0.022	0.339TrpGln: 0.339 ± 0.027	0.544TrpArg: 0.544 ± 0.029	0.504TrpSer: 0.504 ± 0.033	0.533TrpThr: 0.533 ± 0.03	0.433TrpVal: 0.433 ± 0.028	0.091TrpTrp: 0.091 ± 0.014	0.346TrpTyr: 0.346 ± 0.03	0.002TrpXaa: 0.002 ± 0.002
Tyr	3.57TyrAla: 3.57 ± 0.095	0.657TyrCys: 0.657 ± 0.036	2.167TyrAsp: 2.167 ± 0.066	2.127TyrGlu: 2.127 ± 0.065	1.398TyrPhe: 1.398 ± 0.05	2.726TyrGly: 2.726 ± 0.074	0.63TyrHis: 0.63 ± 0.037	1.879TyrIle: 1.879 ± 0.059	1.142TyrLys: 1.142 ± 0.048	3.408TyrLeu: 3.408 ± 0.078	0.854TyrMet: 0.854 ± 0.038	1.227TyrAsn: 1.227 ± 0.053	1.387TyrPro: 1.387 ± 0.057	1.009TyrGln: 1.009 ± 0.04	2.369TyrArg: 2.369 ± 0.068	1.777TyrSer: 1.777 ± 0.054	2.114TyrThr: 2.114 ± 0.075	2.201TyrVal: 2.201 ± 0.063	0.371TyrTrp: 0.371 ± 0.024	1.318TyrTyr: 1.318 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.005XaaAla: 0.005 ± 0.003	0.002XaaCys: 0.002 ± 0.002	0.004XaaAsp: 0.004 ± 0.003	0.005XaaGlu: 0.005 ± 0.003	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.002	0.004XaaLys: 0.004 ± 0.002	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.002	0.002XaaPro: 0.002 ± 0.002	0.002XaaGln: 0.002 ± 0.002	0.004XaaArg: 0.004 ± 0.002	0.004XaaSer: 0.004 ± 0.003	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.038XaaXaa: 0.038 ± 0.011

Statistics based on 1915 proteins (549254 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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