Amino acid dipepetide frequency for Peptoclostridium litorale DSM 5388

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.77AlaAla: 5.77 ± 0.122
0.888AlaCys: 0.888 ± 0.035
3.636AlaAsp: 3.636 ± 0.068
3.956AlaGlu: 3.956 ± 0.071
2.943AlaPhe: 2.943 ± 0.06
5.336AlaGly: 5.336 ± 0.092
1.135AlaHis: 1.135 ± 0.038
5.731AlaIle: 5.731 ± 0.096
5.02AlaLys: 5.02 ± 0.082
6.49AlaLeu: 6.49 ± 0.092
2.236AlaMet: 2.236 ± 0.049
2.625AlaAsn: 2.625 ± 0.059
1.617AlaPro: 1.617 ± 0.046
2.294AlaGln: 2.294 ± 0.059
2.705AlaArg: 2.705 ± 0.051
4.331AlaSer: 4.331 ± 0.082
2.662AlaThr: 2.662 ± 0.078
5.913AlaVal: 5.913 ± 0.092
0.375AlaTrp: 0.375 ± 0.02
2.363AlaTyr: 2.363 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.776CysAla: 0.776 ± 0.028
0.186CysCys: 0.186 ± 0.015
0.682CysAsp: 0.682 ± 0.027
0.809CysGlu: 0.809 ± 0.034
0.393CysPhe: 0.393 ± 0.023
1.119CysGly: 1.119 ± 0.042
0.211CysHis: 0.211 ± 0.015
1.003CysIle: 1.003 ± 0.037
0.831CysLys: 0.831 ± 0.033
0.683CysLeu: 0.683 ± 0.024
0.366CysMet: 0.366 ± 0.021
0.472CysAsn: 0.472 ± 0.023
0.489CysPro: 0.489 ± 0.028
0.215CysGln: 0.215 ± 0.017
0.502CysArg: 0.502 ± 0.023
0.93CysSer: 0.93 ± 0.032
0.56CysThr: 0.56 ± 0.026
0.721CysVal: 0.721 ± 0.028
0.078CysTrp: 0.078 ± 0.01
0.286CysTyr: 0.286 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.493AspAla: 3.493 ± 0.07
0.577AspCys: 0.577 ± 0.026
2.957AspAsp: 2.957 ± 0.073
5.542AspGlu: 5.542 ± 0.093
2.985AspPhe: 2.985 ± 0.061
4.112AspGly: 4.112 ± 0.09
0.742AspHis: 0.742 ± 0.033
6.808AspIle: 6.808 ± 0.099
3.944AspLys: 3.944 ± 0.064
3.961AspLeu: 3.961 ± 0.072
2.463AspMet: 2.463 ± 0.048
2.045AspAsn: 2.045 ± 0.044
1.64AspPro: 1.64 ± 0.044
1.035AspGln: 1.035 ± 0.031
2.35AspArg: 2.35 ± 0.059
3.802AspSer: 3.802 ± 0.079
2.355AspThr: 2.355 ± 0.055
4.435AspVal: 4.435 ± 0.078
0.404AspTrp: 0.404 ± 0.021
2.41AspTyr: 2.41 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
4.465GluAla: 4.465 ± 0.078
0.715GluCys: 0.715 ± 0.035
4.742GluAsp: 4.742 ± 0.081
6.439GluGlu: 6.439 ± 0.106
3.215GluPhe: 3.215 ± 0.059
5.27GluGly: 5.27 ± 0.087
1.229GluHis: 1.229 ± 0.038
7.251GluIle: 7.251 ± 0.102
8.242GluLys: 8.242 ± 0.115
6.46GluLeu: 6.46 ± 0.089
2.608GluMet: 2.608 ± 0.062
5.193GluAsn: 5.193 ± 0.077
1.629GluPro: 1.629 ± 0.053
2.011GluGln: 2.011 ± 0.056
3.282GluArg: 3.282 ± 0.065
4.781GluSer: 4.781 ± 0.09
2.915GluThr: 2.915 ± 0.06
4.632GluVal: 4.632 ± 0.076
0.458GluTrp: 0.458 ± 0.023
3.274GluTyr: 3.274 ± 0.066
0.0GluXaa: 0.0 ± 0.0
Phe
2.944PheAla: 2.944 ± 0.068
0.493PheCys: 0.493 ± 0.022
2.533PheAsp: 2.533 ± 0.049
3.88PheGlu: 3.88 ± 0.067
1.875PhePhe: 1.875 ± 0.049
3.111PheGly: 3.111 ± 0.061
0.546PheHis: 0.546 ± 0.022
3.651PheIle: 3.651 ± 0.069
3.252PheLys: 3.252 ± 0.057
3.46PheLeu: 3.46 ± 0.073
1.438PheMet: 1.438 ± 0.041
1.873PheAsn: 1.873 ± 0.042
1.215PhePro: 1.215 ± 0.039
0.771PheGln: 0.771 ± 0.031
1.364PheArg: 1.364 ± 0.043
3.236PheSer: 3.236 ± 0.069
2.09PheThr: 2.09 ± 0.053
2.867PheVal: 2.867 ± 0.065
0.326PheTrp: 0.326 ± 0.02
1.558PheTyr: 1.558 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
5.142GlyAla: 5.142 ± 0.106
1.044GlyCys: 1.044 ± 0.034
3.923GlyAsp: 3.923 ± 0.072
5.423GlyGlu: 5.423 ± 0.079
3.559GlyPhe: 3.559 ± 0.069
5.154GlyGly: 5.154 ± 0.099
1.27GlyHis: 1.27 ± 0.038
7.564GlyIle: 7.564 ± 0.1
5.841GlyLys: 5.841 ± 0.086
6.016GlyLeu: 6.016 ± 0.095
2.639GlyMet: 2.639 ± 0.055
2.811GlyAsn: 2.811 ± 0.059
1.448GlyPro: 1.448 ± 0.042
1.83GlyGln: 1.83 ± 0.046
3.068GlyArg: 3.068 ± 0.066
4.517GlySer: 4.517 ± 0.07
3.492GlyThr: 3.492 ± 0.069
5.529GlyVal: 5.529 ± 0.093
0.565GlyTrp: 0.565 ± 0.028
3.254GlyTyr: 3.254 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
0.967HisAla: 0.967 ± 0.036
0.215HisCys: 0.215 ± 0.016
0.795HisAsp: 0.795 ± 0.029
1.199HisGlu: 1.199 ± 0.038
0.724HisPhe: 0.724 ± 0.033
1.213HisGly: 1.213 ± 0.04
0.303HisHis: 0.303 ± 0.02
1.576HisIle: 1.576 ± 0.037
0.95HisLys: 0.95 ± 0.037
1.118HisLeu: 1.118 ± 0.04
0.636HisMet: 0.636 ± 0.028
0.609HisAsn: 0.609 ± 0.028
0.796HisPro: 0.796 ± 0.033
0.308HisGln: 0.308 ± 0.017
0.689HisArg: 0.689 ± 0.032
1.142HisSer: 1.142 ± 0.034
0.715HisThr: 0.715 ± 0.032
1.079HisVal: 1.079 ± 0.037
0.089HisTrp: 0.089 ± 0.011
0.584HisTyr: 0.584 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
6.212IleAla: 6.212 ± 0.096
1.115IleCys: 1.115 ± 0.037
5.733IleAsp: 5.733 ± 0.084
7.65IleGlu: 7.65 ± 0.12
3.58IlePhe: 3.58 ± 0.075
6.414IleGly: 6.414 ± 0.09
1.245IleHis: 1.245 ± 0.039
6.934IleIle: 6.934 ± 0.101
6.7IleLys: 6.7 ± 0.095
6.926IleLeu: 6.926 ± 0.107
2.787IleMet: 2.787 ± 0.044
3.753IleAsn: 3.753 ± 0.073
3.071IlePro: 3.071 ± 0.058
2.29IleGln: 2.29 ± 0.057
3.529IleArg: 3.529 ± 0.071
6.953IleSer: 6.953 ± 0.105
3.987IleThr: 3.987 ± 0.064
6.477IleVal: 6.477 ± 0.096
0.558IleTrp: 0.558 ± 0.029
2.957IleTyr: 2.957 ± 0.067
0.0IleXaa: 0.0 ± 0.0
Lys
5.155LysAla: 5.155 ± 0.081
0.725LysCys: 0.725 ± 0.031
4.75LysAsp: 4.75 ± 0.077
6.444LysGlu: 6.444 ± 0.091
2.553LysPhe: 2.553 ± 0.059
5.596LysGly: 5.596 ± 0.068
1.107LysHis: 1.107 ± 0.04
6.495LysIle: 6.495 ± 0.09
7.333LysLys: 7.333 ± 0.112
6.257LysLeu: 6.257 ± 0.077
2.705LysMet: 2.705 ± 0.053
4.515LysAsn: 4.515 ± 0.076
1.984LysPro: 1.984 ± 0.047
1.782LysGln: 1.782 ± 0.049
3.859LysArg: 3.859 ± 0.061
5.39LysSer: 5.39 ± 0.081
3.568LysThr: 3.568 ± 0.065
4.747LysVal: 4.747 ± 0.08
0.533LysTrp: 0.533 ± 0.024
3.003LysTyr: 3.003 ± 0.062
0.0LysXaa: 0.0 ± 0.0
Leu
5.471LeuAla: 5.471 ± 0.088
0.953LeuCys: 0.953 ± 0.029
4.741LeuAsp: 4.741 ± 0.076
6.789LeuGlu: 6.789 ± 0.096
3.376LeuPhe: 3.376 ± 0.074
6.195LeuGly: 6.195 ± 0.106
1.189LeuHis: 1.189 ± 0.04
6.266LeuIle: 6.266 ± 0.1
6.954LeuLys: 6.954 ± 0.095
6.876LeuLeu: 6.876 ± 0.099
2.564LeuMet: 2.564 ± 0.054
3.714LeuAsn: 3.714 ± 0.065
2.588LeuPro: 2.588 ± 0.061
2.098LeuGln: 2.098 ± 0.053
3.315LeuArg: 3.315 ± 0.058
6.85LeuSer: 6.85 ± 0.105
3.751LeuThr: 3.751 ± 0.074
5.451LeuVal: 5.451 ± 0.077
0.531LeuTrp: 0.531 ± 0.03
2.607LeuTyr: 2.607 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
2.684MetAla: 2.684 ± 0.065
0.315MetCys: 0.315 ± 0.019
2.473MetAsp: 2.473 ± 0.053
2.706MetGlu: 2.706 ± 0.055
1.111MetPhe: 1.111 ± 0.037
2.714MetGly: 2.714 ± 0.054
0.539MetHis: 0.539 ± 0.025
2.4MetIle: 2.4 ± 0.051
2.838MetLys: 2.838 ± 0.056
2.884MetLeu: 2.884 ± 0.059
0.841MetMet: 0.841 ± 0.028
1.722MetAsn: 1.722 ± 0.045
1.156MetPro: 1.156 ± 0.038
0.89MetGln: 0.89 ± 0.031
1.151MetArg: 1.151 ± 0.034
2.194MetSer: 2.194 ± 0.049
1.351MetThr: 1.351 ± 0.032
2.03MetVal: 2.03 ± 0.041
0.173MetTrp: 0.173 ± 0.015
0.928MetTyr: 0.928 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.077AsnAla: 3.077 ± 0.067
0.514AsnCys: 0.514 ± 0.025
2.163AsnAsp: 2.163 ± 0.048
3.519AsnGlu: 3.519 ± 0.07
1.746AsnPhe: 1.746 ± 0.04
3.312AsnGly: 3.312 ± 0.06
0.701AsnHis: 0.701 ± 0.027
5.023AsnIle: 5.023 ± 0.076
2.895AsnLys: 2.895 ± 0.062
3.646AsnLeu: 3.646 ± 0.057
1.809AsnMet: 1.809 ± 0.046
1.671AsnAsn: 1.671 ± 0.048
1.984AsnPro: 1.984 ± 0.044
0.999AsnGln: 0.999 ± 0.036
1.882AsnArg: 1.882 ± 0.045
2.985AsnSer: 2.985 ± 0.06
2.118AsnThr: 2.118 ± 0.056
3.315AsnVal: 3.315 ± 0.067
0.354AsnTrp: 0.354 ± 0.02
1.581AsnTyr: 1.581 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
1.992ProAla: 1.992 ± 0.055
0.354ProCys: 0.354 ± 0.019
1.81ProAsp: 1.81 ± 0.047
2.44ProGlu: 2.44 ± 0.057
1.43ProPhe: 1.43 ± 0.037
2.243ProGly: 2.243 ± 0.063
0.623ProHis: 0.623 ± 0.025
2.159ProIle: 2.159 ± 0.042
2.079ProLys: 2.079 ± 0.052
2.58ProLeu: 2.58 ± 0.059
0.75ProMet: 0.75 ± 0.027
1.171ProAsn: 1.171 ± 0.038
0.764ProPro: 0.764 ± 0.036
0.987ProGln: 0.987 ± 0.032
0.95ProArg: 0.95 ± 0.037
1.925ProSer: 1.925 ± 0.045
1.281ProThr: 1.281 ± 0.038
2.792ProVal: 2.792 ± 0.066
0.259ProTrp: 0.259 ± 0.017
1.255ProTyr: 1.255 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
1.691GlnAla: 1.691 ± 0.051
0.215GlnCys: 0.215 ± 0.015
1.219GlnAsp: 1.219 ± 0.041
1.812GlnGlu: 1.812 ± 0.052
0.959GlnPhe: 0.959 ± 0.033
1.64GlnGly: 1.64 ± 0.047
0.42GlnHis: 0.42 ± 0.021
2.367GlnIle: 2.367 ± 0.05
2.458GlnLys: 2.458 ± 0.055
2.137GlnLeu: 2.137 ± 0.046
0.94GlnMet: 0.94 ± 0.032
1.492GlnAsn: 1.492 ± 0.043
0.68GlnPro: 0.68 ± 0.031
0.664GlnGln: 0.664 ± 0.031
1.202GlnArg: 1.202 ± 0.038
1.614GlnSer: 1.614 ± 0.046
1.248GlnThr: 1.248 ± 0.04
1.681GlnVal: 1.681 ± 0.044
0.174GlnTrp: 0.174 ± 0.013
0.831GlnTyr: 0.831 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
2.788ArgAla: 2.788 ± 0.063
0.389ArgCys: 0.389 ± 0.018
2.388ArgAsp: 2.388 ± 0.054
3.56ArgGlu: 3.56 ± 0.069
1.814ArgPhe: 1.814 ± 0.043
2.898ArgGly: 2.898 ± 0.057
0.739ArgHis: 0.739 ± 0.031
3.558ArgIle: 3.558 ± 0.061
2.978ArgLys: 2.978 ± 0.058
3.845ArgLeu: 3.845 ± 0.073
1.272ArgMet: 1.272 ± 0.036
1.718ArgAsn: 1.718 ± 0.043
1.265ArgPro: 1.265 ± 0.035
1.219ArgGln: 1.219 ± 0.043
1.847ArgArg: 1.847 ± 0.056
2.198ArgSer: 2.198 ± 0.053
1.601ArgThr: 1.601 ± 0.043
2.827ArgVal: 2.827 ± 0.053
0.309ArgTrp: 0.309 ± 0.019
1.588ArgTyr: 1.588 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
4.602SerAla: 4.602 ± 0.082
0.79SerCys: 0.79 ± 0.031
3.828SerAsp: 3.828 ± 0.073
4.706SerGlu: 4.706 ± 0.074
3.05SerPhe: 3.05 ± 0.068
5.615SerGly: 5.615 ± 0.071
1.14SerHis: 1.14 ± 0.043
6.665SerIle: 6.665 ± 0.109
5.193SerLys: 5.193 ± 0.078
5.876SerLeu: 5.876 ± 0.093
2.188SerMet: 2.188 ± 0.055
3.154SerAsn: 3.154 ± 0.057
2.145SerPro: 2.145 ± 0.053
2.081SerGln: 2.081 ± 0.057
2.858SerArg: 2.858 ± 0.057
5.117SerSer: 5.117 ± 0.09
2.885SerThr: 2.885 ± 0.058
4.485SerVal: 4.485 ± 0.074
0.528SerTrp: 0.528 ± 0.024
2.382SerTyr: 2.382 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
3.291ThrAla: 3.291 ± 0.073
0.487ThrCys: 0.487 ± 0.028
2.335ThrAsp: 2.335 ± 0.05
2.538ThrGlu: 2.538 ± 0.059
1.911ThrPhe: 1.911 ± 0.054
4.277ThrGly: 4.277 ± 0.077
0.772ThrHis: 0.772 ± 0.028
3.736ThrIle: 3.736 ± 0.063
2.537ThrLys: 2.537 ± 0.054
3.944ThrLeu: 3.944 ± 0.078
1.201ThrMet: 1.201 ± 0.034
1.777ThrAsn: 1.777 ± 0.042
1.735ThrPro: 1.735 ± 0.054
1.165ThrGln: 1.165 ± 0.034
1.741ThrArg: 1.741 ± 0.048
2.894ThrSer: 2.894 ± 0.056
2.031ThrThr: 2.031 ± 0.056
3.608ThrVal: 3.608 ± 0.069
0.293ThrTrp: 0.293 ± 0.018
1.549ThrTyr: 1.549 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
4.787ValAla: 4.787 ± 0.079
0.854ValCys: 0.854 ± 0.035
4.595ValAsp: 4.595 ± 0.083
5.838ValGlu: 5.838 ± 0.081
3.201ValPhe: 3.201 ± 0.061
4.68ValGly: 4.68 ± 0.081
1.118ValHis: 1.118 ± 0.038
5.908ValIle: 5.908 ± 0.087
5.469ValLys: 5.469 ± 0.077
6.091ValLeu: 6.091 ± 0.089
2.162ValMet: 2.162 ± 0.053
3.002ValAsn: 3.002 ± 0.059
2.15ValPro: 2.15 ± 0.049
1.918ValGln: 1.918 ± 0.048
2.608ValArg: 2.608 ± 0.054
5.054ValSer: 5.054 ± 0.082
3.133ValThr: 3.133 ± 0.07
5.347ValVal: 5.347 ± 0.096
0.426ValTrp: 0.426 ± 0.021
2.485ValTyr: 2.485 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.501TrpAla: 0.501 ± 0.027
0.055TrpCys: 0.055 ± 0.008
0.418TrpAsp: 0.418 ± 0.022
0.479TrpGlu: 0.479 ± 0.025
0.315TrpPhe: 0.315 ± 0.022
0.532TrpGly: 0.532 ± 0.026
0.122TrpHis: 0.122 ± 0.012
0.595TrpIle: 0.595 ± 0.027
0.487TrpLys: 0.487 ± 0.023
0.521TrpLeu: 0.521 ± 0.027
0.235TrpMet: 0.235 ± 0.015
0.366TrpAsn: 0.366 ± 0.019
0.184TrpPro: 0.184 ± 0.017
0.184TrpGln: 0.184 ± 0.014
0.265TrpArg: 0.265 ± 0.019
0.41TrpSer: 0.41 ± 0.026
0.354TrpThr: 0.354 ± 0.022
0.415TrpVal: 0.415 ± 0.023
0.077TrpTrp: 0.077 ± 0.01
0.259TrpTyr: 0.259 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.218TyrAla: 2.218 ± 0.052
0.425TyrCys: 0.425 ± 0.022
2.342TyrAsp: 2.342 ± 0.056
3.081TyrGlu: 3.081 ± 0.067
1.743TyrPhe: 1.743 ± 0.045
2.659TyrGly: 2.659 ± 0.049
0.556TyrHis: 0.556 ± 0.025
3.181TyrIle: 3.181 ± 0.059
2.501TyrLys: 2.501 ± 0.061
2.509TyrLeu: 2.509 ± 0.054
1.261TyrMet: 1.261 ± 0.038
1.612TyrAsn: 1.612 ± 0.045
1.3TyrPro: 1.3 ± 0.041
0.702TyrGln: 0.702 ± 0.026
1.636TyrArg: 1.636 ± 0.048
2.965TyrSer: 2.965 ± 0.067
1.723TyrThr: 1.723 ± 0.048
2.474TyrVal: 2.474 ± 0.058
0.264TyrTrp: 0.264 ± 0.015
1.521TyrTyr: 1.521 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2948 proteins (924342 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski