Infectious bronchitis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Gammacoronavirus; Igacovirus; Avian coronavirus

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9J4A9|Q9J4A9_9GAMC Accessory protein 3a OS=Infectious bronchitis virus OX=11120 GN=3a PE=4 SV=1
MM1 pKa = 7.6LNLEE5 pKa = 4.9AIIEE9 pKa = 4.32TGDD12 pKa = 3.44QVIQKK17 pKa = 10.01ISFNLQHH24 pKa = 6.73ISSVLNTEE32 pKa = 3.84VFDD35 pKa = 4.52PFDD38 pKa = 3.29YY39 pKa = 10.68CYY41 pKa = 11.2YY42 pKa = 10.31RR43 pKa = 11.84GGNFWEE49 pKa = 4.43IEE51 pKa = 4.21SAEE54 pKa = 4.26DD55 pKa = 4.39CSGDD59 pKa = 4.48DD60 pKa = 4.1EE61 pKa = 5.77FIEE64 pKa = 4.42

Molecular weight: 7.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9J4A7|Q9J4A7_9GAMC Envelope small membrane protein OS=Infectious bronchitis virus OX=11120 GN=3c PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 9.77WLTSFGRR9 pKa = 11.84AVISCYY15 pKa = 10.12KK16 pKa = 10.68ALLLTQLRR24 pKa = 11.84VLDD27 pKa = 4.68RR28 pKa = 11.84LILDD32 pKa = 4.38HH33 pKa = 6.65GPKK36 pKa = 9.86RR37 pKa = 11.84VLTCGRR43 pKa = 11.84RR44 pKa = 11.84VLLSQLDD51 pKa = 3.76LVYY54 pKa = 10.83RR55 pKa = 11.84LAYY58 pKa = 9.49TPTQSLVV65 pKa = 3.06

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

8783

57

6610

975.9

109.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.672 ± 0.281

3.222 ± 0.311

5.545 ± 0.809

4.566 ± 0.54

5.579 ± 0.36

6.171 ± 0.546

1.799 ± 0.394

5.317 ± 0.625

6.046 ± 0.831

8.562 ± 0.775

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.776 ± 0.162

5.636 ± 0.576

3.883 ± 0.473

3.655 ± 0.75

3.643 ± 0.897

6.809 ± 0.786

5.966 ± 0.506

9.074 ± 1.334

1.344 ± 0.292

4.736 ± 0.445
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski