Rodent Torque teno virus 1
Average proteome isoelectric point is 7.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X2G8B9|X2G8B9_9VIRU ORF3 OS=Rodent Torque teno virus 1 OX=1514664 PE=4 SV=1
MM1 pKa = 7.23 NVDD4 pKa = 2.66 EE5 pKa = 4.83 DD6 pKa = 4.48 FIYY9 pKa = 10.85 RR10 pKa = 11.84 EE11 pKa = 4.12 DD12 pKa = 3.36 SWLCMIKK19 pKa = 8.47 QTHH22 pKa = 5.99 EE23 pKa = 4.17 LFCNCTSWTKK33 pKa = 10.31 HH34 pKa = 5.57 LKK36 pKa = 8.96 TLLDD40 pKa = 3.97 FNHH43 pKa = 6.94 PEE45 pKa = 3.84 WRR47 pKa = 11.84 GDD49 pKa = 3.79 SQDD52 pKa = 3.6 EE53 pKa = 4.23 QPGKK57 pKa = 10.26 EE58 pKa = 4.01 KK59 pKa = 10.76 PGDD62 pKa = 3.7 NPEE65 pKa = 4.21 GVPTDD70 pKa = 3.68 EE71 pKa = 4.4 EE72 pKa = 4.91 LIGALEE78 pKa = 3.84 EE79 pKa = 4.61 LEE81 pKa = 4.18 NGGAGEE87 pKa = 4.21 GTSRR91 pKa = 11.84 SEE93 pKa = 4.09 CSLKK97 pKa = 10.9 YY98 pKa = 10.87 SLDD101 pKa = 3.45 TLGDD105 pKa = 3.73 VLSEE109 pKa = 4.07 ALCVLSS115 pKa = 4.57
Molecular weight: 12.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.227
IPC2_protein 4.24
IPC_protein 4.151
Toseland 3.986
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 3.986
Rodwell 3.999
Grimsley 3.897
Solomon 4.101
Lehninger 4.062
Nozaki 4.228
DTASelect 4.368
Thurlkill 4.012
EMBOSS 3.999
Sillero 4.266
Patrickios 3.312
IPC_peptide 4.113
IPC2_peptide 4.253
IPC2.peptide.svr19 4.189
Protein with the highest isoelectric point:
>tr|X2G9U7|X2G9U7_9VIRU Capsid protein OS=Rodent Torque teno virus 1 OX=1514664 PE=3 SV=1
MM1 pKa = 7.63 AWRR4 pKa = 11.84 FPRR7 pKa = 11.84 RR8 pKa = 11.84 TTWKK12 pKa = 9.66 RR13 pKa = 11.84 KK14 pKa = 5.77 TWRR17 pKa = 11.84 QPRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 TYY24 pKa = 10.0 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 AYY29 pKa = 10.18 RR30 pKa = 11.84 RR31 pKa = 11.84 TRR33 pKa = 11.84 RR34 pKa = 11.84 TRR36 pKa = 11.84 KK37 pKa = 7.06 WRR39 pKa = 11.84 RR40 pKa = 11.84 GRR42 pKa = 11.84 RR43 pKa = 11.84 NLKK46 pKa = 9.82 VRR48 pKa = 11.84 VLTEE52 pKa = 3.35 IQPRR56 pKa = 11.84 YY57 pKa = 7.46 TRR59 pKa = 11.84 RR60 pKa = 11.84 CVIRR64 pKa = 11.84 GIMCPLIVGQPNADD78 pKa = 3.34 GSPSKK83 pKa = 10.8 QNWEE87 pKa = 3.96 LRR89 pKa = 11.84 QGGIFAYY96 pKa = 9.74 KK97 pKa = 10.08 HH98 pKa = 6.53 DD99 pKa = 5.29 GSDD102 pKa = 2.78 LWMGTWSMGMVSLSILYY119 pKa = 10.29 GEE121 pKa = 4.23 HH122 pKa = 4.63 QAYY125 pKa = 9.52 RR126 pKa = 11.84 NRR128 pKa = 11.84 WSVSNCGFDD137 pKa = 3.15 MVEE140 pKa = 3.87 YY141 pKa = 10.35 RR142 pKa = 11.84 GTTIYY147 pKa = 10.8 LEE149 pKa = 4.08 QHH151 pKa = 5.0 STMDD155 pKa = 3.65 YY156 pKa = 10.57 IVFFDD161 pKa = 3.66 EE162 pKa = 4.72 EE163 pKa = 4.41 YY164 pKa = 11.15 RR165 pKa = 11.84 DD166 pKa = 3.53 ITSFAAQASLHH177 pKa = 6.2 PLVLMTHH184 pKa = 7.4 PKK186 pKa = 9.56 TILIKK191 pKa = 9.96 SRR193 pKa = 11.84 EE194 pKa = 3.98 RR195 pKa = 11.84 AGPRR199 pKa = 11.84 RR200 pKa = 11.84 ARR202 pKa = 11.84 KK203 pKa = 9.73 VFIPRR208 pKa = 11.84 PSWWDD213 pKa = 3.44 SGWKK217 pKa = 9.58 FSKK220 pKa = 10.53 DD221 pKa = 3.46 VCNKK225 pKa = 10.26 GLFVWYY231 pKa = 9.85 ISAIDD236 pKa = 4.11 MEE238 pKa = 4.98 HH239 pKa = 6.9 PWMKK243 pKa = 10.57 AYY245 pKa = 10.44 VGNKK249 pKa = 8.57 TDD251 pKa = 4.03 FQNLMWWRR259 pKa = 11.84 NNKK262 pKa = 8.4 WKK264 pKa = 10.54 EE265 pKa = 3.96 DD266 pKa = 2.96 FDD268 pKa = 4.27 TYY270 pKa = 11.55 VRR272 pKa = 11.84 TTAQMLQTSKK282 pKa = 11.22 NDD284 pKa = 3.31 NNYY287 pKa = 8.53 KK288 pKa = 9.64 TEE290 pKa = 4.06 TATGPFMLSIKK301 pKa = 10.09 DD302 pKa = 3.62 RR303 pKa = 11.84 NRR305 pKa = 11.84 ATVNQQFTWFYY316 pKa = 10.06 KK317 pKa = 10.54 SYY319 pKa = 9.11 WHH321 pKa = 7.09 WGGNNLTIKK330 pKa = 10.21 KK331 pKa = 9.35 VCNPNMDD338 pKa = 3.43 VPITT342 pKa = 3.68
Molecular weight: 41.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.823
IPC_protein 10.584
Toseland 10.599
ProMoST 10.306
Dawson 10.745
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 10.994
Grimsley 10.804
Solomon 10.818
Lehninger 10.774
Nozaki 10.584
DTASelect 10.452
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.657
Patrickios 10.657
IPC_peptide 10.818
IPC2_peptide 9.428
IPC2.peptide.svr19 8.474
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
685
83
342
171.3
20.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.65 ± 0.927
2.19 ± 0.595
5.109 ± 0.82
6.277 ± 2.202
3.358 ± 0.451
5.985 ± 0.758
2.336 ± 0.424
4.088 ± 0.917
7.591 ± 1.039
6.861 ± 1.593
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.212 ± 0.574
4.964 ± 0.378
4.818 ± 0.398
4.818 ± 0.916
8.905 ± 2.127
7.153 ± 1.907
7.153 ± 0.699
4.38 ± 0.555
4.234 ± 0.839
2.92 ± 1.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here