Oenococcus phage phiOE33PA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U9PDS7|A0A2U9PDS7_9CAUD Uncharacterized protein OS=Oenococcus phage phiOE33PA OX=2201414 GN=phiOE33PA_00190 PE=4 SV=1
MM1 pKa = 8.04AITMYY6 pKa = 10.52QADD9 pKa = 4.53RR10 pKa = 11.84NFVSPANDD18 pKa = 3.22AALYY22 pKa = 10.41SAILNNTSGVLANRR36 pKa = 11.84GNNFALTIDD45 pKa = 3.99GLVVSIDD52 pKa = 3.11TGQAVIGGRR61 pKa = 11.84LIEE64 pKa = 4.1ITALEE69 pKa = 4.67SVTVPANSSGSICLVVDD86 pKa = 3.84LTKK89 pKa = 10.91TNTVTGNAGDD99 pKa = 3.77TTYY102 pKa = 11.28SVAVNQVYY110 pKa = 8.69TSAVTGSLTQDD121 pKa = 3.73DD122 pKa = 4.94LNDD125 pKa = 3.54GGFIYY130 pKa = 9.8EE131 pKa = 4.5LPLASFVSTATSVTLTDD148 pKa = 3.23TTGYY152 pKa = 11.07LNDD155 pKa = 4.48TGWLTLPNATGLVIGSGGFTKK176 pKa = 10.67YY177 pKa = 9.58RR178 pKa = 11.84VKK180 pKa = 11.29NNVVYY185 pKa = 10.51IRR187 pKa = 11.84LQALDD192 pKa = 3.51TSKK195 pKa = 7.9TTNANQIGTIPSKK208 pKa = 10.1YY209 pKa = 9.79APSITFMAAGMDD221 pKa = 3.97DD222 pKa = 4.05SSGTPYY228 pKa = 10.75GIEE231 pKa = 3.79LHH233 pKa = 6.0VQTSGLIYY241 pKa = 10.28ANYY244 pKa = 9.79VSPHH248 pKa = 5.81NGGIGGTITYY258 pKa = 9.68PLGG261 pKa = 3.64

Molecular weight: 27.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U9PF94|A0A2U9PF94_9CAUD HTH-type transcriptional regulator OS=Oenococcus phage phiOE33PA OX=2201414 GN=xre PE=4 SV=1
MM1 pKa = 7.22NVVFLIVIGLIVGTFVILFGGGGAAIYY28 pKa = 10.33LGILTGVVGLNASTAASTSLVTVLPSLILGVWTYY62 pKa = 11.09YY63 pKa = 10.3RR64 pKa = 11.84QGTIKK69 pKa = 9.7TRR71 pKa = 11.84LGNQMLITAIPAVIIGSLISSYY93 pKa = 10.99IPDD96 pKa = 4.33NLYY99 pKa = 10.36KK100 pKa = 10.1WIVGIILILLGINMLFQKK118 pKa = 10.25QKK120 pKa = 9.7SQVDD124 pKa = 3.73PVMSKK129 pKa = 10.4QINRR133 pKa = 11.84KK134 pKa = 9.37DD135 pKa = 3.29RR136 pKa = 11.84FKK138 pKa = 11.37AGIFGIIGGLMVGVAGMSGGAPIIAGLFLIGLSTVNAVATSAYY181 pKa = 10.36VLVFMSAIGTVFHH194 pKa = 7.02IVGSQVDD201 pKa = 3.85WNAGISLMVGALIGAAIGPRR221 pKa = 11.84LLTRR225 pKa = 11.84LTKK228 pKa = 10.65SKK230 pKa = 9.79VGKK233 pKa = 9.78YY234 pKa = 8.8IKK236 pKa = 10.08PVIAFFLVLLGFKK249 pKa = 9.45TLFF252 pKa = 3.78

Molecular weight: 26.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12485

44

1613

219.0

24.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.048 ± 0.483

0.481 ± 0.109

6.552 ± 0.326

5.038 ± 0.341

4.189 ± 0.316

6.063 ± 0.445

1.858 ± 0.175

7.345 ± 0.372

8.474 ± 0.569

8.634 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.134

6.159 ± 0.254

2.883 ± 0.245

4.405 ± 0.253

3.148 ± 0.223

7.577 ± 0.454

6.696 ± 0.386

5.983 ± 0.273

1.097 ± 0.131

4.125 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski