Amino acid dipepetide frequency for Microbacterium phage PhriedRice

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.969AlaAla: 10.969 ± 1.524
0.293AlaCys: 0.293 ± 0.189
6.863AlaAsp: 6.863 ± 0.575
6.98AlaGlu: 6.98 ± 0.787
2.874AlaPhe: 2.874 ± 0.38
6.335AlaGly: 6.335 ± 0.718
1.349AlaHis: 1.349 ± 0.269
4.047AlaIle: 4.047 ± 0.365
2.874AlaLys: 2.874 ± 0.508
7.743AlaLeu: 7.743 ± 0.532
2.229AlaMet: 2.229 ± 0.417
4.399AlaAsn: 4.399 ± 0.596
3.989AlaPro: 3.989 ± 0.453
5.103AlaGln: 5.103 ± 0.688
6.1AlaArg: 6.1 ± 0.596
6.218AlaSer: 6.218 ± 0.6
5.983AlaThr: 5.983 ± 0.676
5.924AlaVal: 5.924 ± 0.707
1.701AlaTrp: 1.701 ± 0.37
3.226AlaTyr: 3.226 ± 0.388
0.0AlaXaa: 0.0 ± 0.0
Cys
0.293CysAla: 0.293 ± 0.153
0.0CysCys: 0.0 ± 0.0
0.117CysAsp: 0.117 ± 0.096
0.293CysGlu: 0.293 ± 0.171
0.352CysPhe: 0.352 ± 0.207
0.469CysGly: 0.469 ± 0.231
0.235CysHis: 0.235 ± 0.147
0.059CysIle: 0.059 ± 0.078
0.176CysLys: 0.176 ± 0.12
0.411CysLeu: 0.411 ± 0.239
0.176CysMet: 0.176 ± 0.14
0.059CysAsn: 0.059 ± 0.059
0.293CysPro: 0.293 ± 0.205
0.059CysGln: 0.059 ± 0.06
0.176CysArg: 0.176 ± 0.122
0.293CysSer: 0.293 ± 0.157
0.352CysThr: 0.352 ± 0.174
0.528CysVal: 0.528 ± 0.262
0.059CysTrp: 0.059 ± 0.064
0.235CysTyr: 0.235 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
6.922AspAla: 6.922 ± 0.949
0.469AspCys: 0.469 ± 0.211
4.282AspAsp: 4.282 ± 0.563
6.1AspGlu: 6.1 ± 0.789
2.17AspPhe: 2.17 ± 0.381
5.514AspGly: 5.514 ± 0.444
1.173AspHis: 1.173 ± 0.221
3.461AspIle: 3.461 ± 0.379
3.695AspLys: 3.695 ± 0.563
5.338AspLeu: 5.338 ± 0.473
1.525AspMet: 1.525 ± 0.32
2.64AspAsn: 2.64 ± 0.319
3.285AspPro: 3.285 ± 0.488
2.992AspGln: 2.992 ± 0.416
3.461AspArg: 3.461 ± 0.389
2.816AspSer: 2.816 ± 0.371
3.871AspThr: 3.871 ± 0.39
3.168AspVal: 3.168 ± 0.49
1.877AspTrp: 1.877 ± 0.329
1.584AspTyr: 1.584 ± 0.335
0.0AspXaa: 0.0 ± 0.0
Glu
6.218GluAla: 6.218 ± 0.636
0.059GluCys: 0.059 ± 0.069
4.634GluAsp: 4.634 ± 0.71
4.106GluGlu: 4.106 ± 0.776
2.053GluPhe: 2.053 ± 0.283
4.693GluGly: 4.693 ± 0.541
1.115GluHis: 1.115 ± 0.273
2.816GluIle: 2.816 ± 0.55
3.226GluLys: 3.226 ± 0.573
6.98GluLeu: 6.98 ± 0.654
1.701GluMet: 1.701 ± 0.365
3.226GluAsn: 3.226 ± 0.455
1.994GluPro: 1.994 ± 0.392
3.168GluGln: 3.168 ± 0.469
3.989GluArg: 3.989 ± 0.591
3.285GluSer: 3.285 ± 0.347
4.223GluThr: 4.223 ± 0.585
4.458GluVal: 4.458 ± 0.476
1.994GluTrp: 1.994 ± 0.295
2.288GluTyr: 2.288 ± 0.548
0.0GluXaa: 0.0 ± 0.0
Phe
2.288PheAla: 2.288 ± 0.411
0.528PheCys: 0.528 ± 0.239
2.64PheAsp: 2.64 ± 0.37
1.994PheGlu: 1.994 ± 0.5
1.584PhePhe: 1.584 ± 0.318
2.346PheGly: 2.346 ± 0.343
0.352PheHis: 0.352 ± 0.136
1.994PheIle: 1.994 ± 0.383
1.408PheLys: 1.408 ± 0.294
1.701PheLeu: 1.701 ± 0.352
0.997PheMet: 0.997 ± 0.338
1.76PheAsn: 1.76 ± 0.271
1.232PhePro: 1.232 ± 0.366
1.173PheGln: 1.173 ± 0.201
2.053PheArg: 2.053 ± 0.394
2.522PheSer: 2.522 ± 0.301
2.405PheThr: 2.405 ± 0.449
2.112PheVal: 2.112 ± 0.349
0.469PheTrp: 0.469 ± 0.151
0.763PheTyr: 0.763 ± 0.17
0.0PheXaa: 0.0 ± 0.0
Gly
7.039GlyAla: 7.039 ± 0.915
0.117GlyCys: 0.117 ± 0.081
4.575GlyAsp: 4.575 ± 0.468
3.695GlyGlu: 3.695 ± 0.444
2.229GlyPhe: 2.229 ± 0.348
7.332GlyGly: 7.332 ± 1.015
0.821GlyHis: 0.821 ± 0.203
4.106GlyIle: 4.106 ± 0.523
3.402GlyLys: 3.402 ± 0.826
6.98GlyLeu: 6.98 ± 0.936
2.112GlyMet: 2.112 ± 0.479
3.109GlyAsn: 3.109 ± 0.465
1.818GlyPro: 1.818 ± 0.267
3.05GlyGln: 3.05 ± 0.486
4.458GlyArg: 4.458 ± 0.607
5.514GlySer: 5.514 ± 0.711
4.751GlyThr: 4.751 ± 0.582
5.279GlyVal: 5.279 ± 0.676
1.466GlyTrp: 1.466 ± 0.259
2.992GlyTyr: 2.992 ± 0.481
0.0GlyXaa: 0.0 ± 0.0
His
1.29HisAla: 1.29 ± 0.21
0.293HisCys: 0.293 ± 0.146
1.173HisAsp: 1.173 ± 0.264
1.056HisGlu: 1.056 ± 0.323
0.411HisPhe: 0.411 ± 0.169
1.349HisGly: 1.349 ± 0.3
0.411HisHis: 0.411 ± 0.19
0.763HisIle: 0.763 ± 0.225
1.232HisLys: 1.232 ± 0.397
0.997HisLeu: 0.997 ± 0.197
0.411HisMet: 0.411 ± 0.243
0.939HisAsn: 0.939 ± 0.24
1.29HisPro: 1.29 ± 0.321
0.528HisGln: 0.528 ± 0.158
0.88HisArg: 0.88 ± 0.266
0.939HisSer: 0.939 ± 0.308
0.645HisThr: 0.645 ± 0.214
1.29HisVal: 1.29 ± 0.323
0.411HisTrp: 0.411 ± 0.169
0.352HisTyr: 0.352 ± 0.131
0.0HisXaa: 0.0 ± 0.0
Ile
5.338IleAla: 5.338 ± 0.572
0.117IleCys: 0.117 ± 0.109
4.047IleAsp: 4.047 ± 0.448
3.519IleGlu: 3.519 ± 0.605
1.29IlePhe: 1.29 ± 0.268
3.285IleGly: 3.285 ± 0.279
1.056IleHis: 1.056 ± 0.171
3.109IleIle: 3.109 ± 0.6
2.757IleLys: 2.757 ± 0.4
3.461IleLeu: 3.461 ± 0.47
1.115IleMet: 1.115 ± 0.2
2.581IleAsn: 2.581 ± 0.296
2.17IlePro: 2.17 ± 0.5
1.936IleGln: 1.936 ± 0.452
2.992IleArg: 2.992 ± 0.364
2.64IleSer: 2.64 ± 0.33
3.637IleThr: 3.637 ± 0.44
3.109IleVal: 3.109 ± 0.49
0.411IleTrp: 0.411 ± 0.155
1.056IleTyr: 1.056 ± 0.219
0.0IleXaa: 0.0 ± 0.0
Lys
4.047LysAla: 4.047 ± 0.577
0.293LysCys: 0.293 ± 0.189
2.874LysAsp: 2.874 ± 0.435
2.405LysGlu: 2.405 ± 0.474
1.29LysPhe: 1.29 ± 0.233
2.992LysGly: 2.992 ± 0.329
0.469LysHis: 0.469 ± 0.174
2.346LysIle: 2.346 ± 0.406
2.405LysLys: 2.405 ± 0.573
3.813LysLeu: 3.813 ± 0.747
1.056LysMet: 1.056 ± 0.361
1.642LysAsn: 1.642 ± 0.31
2.405LysPro: 2.405 ± 0.352
1.936LysGln: 1.936 ± 0.331
2.112LysArg: 2.112 ± 0.554
2.581LysSer: 2.581 ± 0.358
3.109LysThr: 3.109 ± 0.587
3.226LysVal: 3.226 ± 0.475
0.645LysTrp: 0.645 ± 0.219
1.994LysTyr: 1.994 ± 0.437
0.0LysXaa: 0.0 ± 0.0
Leu
7.508LeuAla: 7.508 ± 0.518
0.235LeuCys: 0.235 ± 0.164
5.279LeuAsp: 5.279 ± 0.748
5.631LeuGlu: 5.631 ± 0.738
2.581LeuPhe: 2.581 ± 0.35
6.1LeuGly: 6.1 ± 0.558
1.408LeuHis: 1.408 ± 0.324
4.81LeuIle: 4.81 ± 0.61
3.695LeuLys: 3.695 ± 0.748
6.452LeuLeu: 6.452 ± 0.715
1.936LeuMet: 1.936 ± 0.315
3.989LeuAsn: 3.989 ± 0.438
3.813LeuPro: 3.813 ± 0.587
4.341LeuGln: 4.341 ± 0.597
5.455LeuArg: 5.455 ± 0.46
5.045LeuSer: 5.045 ± 0.524
5.748LeuThr: 5.748 ± 0.682
5.162LeuVal: 5.162 ± 0.631
1.173LeuTrp: 1.173 ± 0.167
2.522LeuTyr: 2.522 ± 0.45
0.0LeuXaa: 0.0 ± 0.0
Met
2.816MetAla: 2.816 ± 0.377
0.117MetCys: 0.117 ± 0.092
1.466MetAsp: 1.466 ± 0.273
1.584MetGlu: 1.584 ± 0.364
1.056MetPhe: 1.056 ± 0.242
1.115MetGly: 1.115 ± 0.264
0.528MetHis: 0.528 ± 0.148
0.763MetIle: 0.763 ± 0.219
0.939MetLys: 0.939 ± 0.304
2.053MetLeu: 2.053 ± 0.35
0.763MetMet: 0.763 ± 0.205
1.173MetAsn: 1.173 ± 0.282
1.525MetPro: 1.525 ± 0.258
1.056MetGln: 1.056 ± 0.318
1.642MetArg: 1.642 ± 0.235
2.112MetSer: 2.112 ± 0.464
2.112MetThr: 2.112 ± 0.282
1.466MetVal: 1.466 ± 0.25
0.352MetTrp: 0.352 ± 0.132
0.645MetTyr: 0.645 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
4.165AsnAla: 4.165 ± 0.528
0.176AsnCys: 0.176 ± 0.118
2.581AsnAsp: 2.581 ± 0.308
2.522AsnGlu: 2.522 ± 0.354
1.642AsnPhe: 1.642 ± 0.25
3.93AsnGly: 3.93 ± 0.461
0.235AsnHis: 0.235 ± 0.157
2.229AsnIle: 2.229 ± 0.288
2.053AsnLys: 2.053 ± 0.398
2.874AsnLeu: 2.874 ± 0.363
0.763AsnMet: 0.763 ± 0.225
2.64AsnAsn: 2.64 ± 0.418
3.285AsnPro: 3.285 ± 0.496
1.936AsnGln: 1.936 ± 0.286
2.346AsnArg: 2.346 ± 0.412
2.933AsnSer: 2.933 ± 0.415
3.578AsnThr: 3.578 ± 0.482
3.93AsnVal: 3.93 ± 0.317
1.056AsnTrp: 1.056 ± 0.223
1.76AsnTyr: 1.76 ± 0.363
0.0AsnXaa: 0.0 ± 0.0
Pro
4.575ProAla: 4.575 ± 0.802
0.059ProCys: 0.059 ± 0.07
3.461ProAsp: 3.461 ± 0.468
4.751ProGlu: 4.751 ± 0.525
1.349ProPhe: 1.349 ± 0.266
3.109ProGly: 3.109 ± 0.411
0.704ProHis: 0.704 ± 0.204
2.112ProIle: 2.112 ± 0.541
1.936ProLys: 1.936 ± 0.334
2.874ProLeu: 2.874 ± 0.429
1.173ProMet: 1.173 ± 0.307
2.17ProAsn: 2.17 ± 0.38
1.349ProPro: 1.349 ± 0.311
1.525ProGln: 1.525 ± 0.215
2.053ProArg: 2.053 ± 0.44
3.871ProSer: 3.871 ± 0.541
3.168ProThr: 3.168 ± 0.576
2.816ProVal: 2.816 ± 0.336
1.173ProTrp: 1.173 ± 0.206
1.29ProTyr: 1.29 ± 0.199
0.0ProXaa: 0.0 ± 0.0
Gln
5.455GlnAla: 5.455 ± 0.533
0.352GlnCys: 0.352 ± 0.171
2.581GlnAsp: 2.581 ± 0.446
2.992GlnGlu: 2.992 ± 0.435
1.056GlnPhe: 1.056 ± 0.163
2.464GlnGly: 2.464 ± 0.45
1.173GlnHis: 1.173 ± 0.245
2.581GlnIle: 2.581 ± 0.347
1.642GlnLys: 1.642 ± 0.355
3.637GlnLeu: 3.637 ± 0.462
1.642GlnMet: 1.642 ± 0.396
2.405GlnAsn: 2.405 ± 0.337
2.053GlnPro: 2.053 ± 0.388
2.346GlnGln: 2.346 ± 0.499
2.698GlnArg: 2.698 ± 0.324
2.288GlnSer: 2.288 ± 0.38
3.754GlnThr: 3.754 ± 1.097
2.229GlnVal: 2.229 ± 0.375
0.821GlnTrp: 0.821 ± 0.251
1.408GlnTyr: 1.408 ± 0.212
0.0GlnXaa: 0.0 ± 0.0
Arg
5.221ArgAla: 5.221 ± 0.583
0.235ArgCys: 0.235 ± 0.177
3.754ArgAsp: 3.754 ± 0.645
4.341ArgGlu: 4.341 ± 0.55
2.288ArgPhe: 2.288 ± 0.481
4.927ArgGly: 4.927 ± 0.691
0.88ArgHis: 0.88 ± 0.228
3.05ArgIle: 3.05 ± 0.59
1.877ArgLys: 1.877 ± 0.33
4.869ArgLeu: 4.869 ± 0.464
2.405ArgMet: 2.405 ± 0.497
3.109ArgAsn: 3.109 ± 0.436
2.17ArgPro: 2.17 ± 0.35
3.695ArgGln: 3.695 ± 0.502
3.578ArgArg: 3.578 ± 0.811
3.578ArgSer: 3.578 ± 0.698
3.402ArgThr: 3.402 ± 0.464
4.047ArgVal: 4.047 ± 0.504
0.763ArgTrp: 0.763 ± 0.236
1.349ArgTyr: 1.349 ± 0.234
0.0ArgXaa: 0.0 ± 0.0
Ser
5.631SerAla: 5.631 ± 0.596
0.411SerCys: 0.411 ± 0.191
3.871SerAsp: 3.871 ± 0.482
3.695SerGlu: 3.695 ± 0.561
2.053SerPhe: 2.053 ± 0.444
5.162SerGly: 5.162 ± 0.752
1.173SerHis: 1.173 ± 0.285
3.285SerIle: 3.285 ± 0.517
2.17SerLys: 2.17 ± 0.301
5.748SerLeu: 5.748 ± 0.713
1.936SerMet: 1.936 ± 0.333
2.522SerAsn: 2.522 ± 0.538
2.816SerPro: 2.816 ± 0.374
2.405SerGln: 2.405 ± 0.441
3.461SerArg: 3.461 ± 0.393
4.047SerSer: 4.047 ± 0.783
4.81SerThr: 4.81 ± 0.57
3.637SerVal: 3.637 ± 0.342
0.88SerTrp: 0.88 ± 0.34
2.17SerTyr: 2.17 ± 0.244
0.0SerXaa: 0.0 ± 0.0
Thr
6.863ThrAla: 6.863 ± 0.648
0.235ThrCys: 0.235 ± 0.14
3.578ThrAsp: 3.578 ± 0.403
4.165ThrGlu: 4.165 ± 0.535
2.522ThrPhe: 2.522 ± 0.327
6.511ThrGly: 6.511 ± 0.985
1.173ThrHis: 1.173 ± 0.411
2.816ThrIle: 2.816 ± 0.288
2.112ThrLys: 2.112 ± 0.368
6.57ThrLeu: 6.57 ± 0.6
0.821ThrMet: 0.821 ± 0.155
3.168ThrAsn: 3.168 ± 0.42
4.458ThrPro: 4.458 ± 0.567
2.698ThrGln: 2.698 ± 0.473
4.282ThrArg: 4.282 ± 0.447
4.047ThrSer: 4.047 ± 0.513
4.81ThrThr: 4.81 ± 0.523
4.634ThrVal: 4.634 ± 0.592
0.821ThrTrp: 0.821 ± 0.245
1.994ThrTyr: 1.994 ± 0.42
0.0ThrXaa: 0.0 ± 0.0
Val
5.573ValAla: 5.573 ± 0.58
0.293ValCys: 0.293 ± 0.161
5.045ValAsp: 5.045 ± 0.617
3.402ValGlu: 3.402 ± 0.503
1.818ValPhe: 1.818 ± 0.292
3.871ValGly: 3.871 ± 0.499
1.408ValHis: 1.408 ± 0.306
2.874ValIle: 2.874 ± 0.351
3.05ValLys: 3.05 ± 0.592
5.807ValLeu: 5.807 ± 0.638
1.525ValMet: 1.525 ± 0.335
2.757ValAsn: 2.757 ± 0.551
4.106ValPro: 4.106 ± 0.577
2.992ValGln: 2.992 ± 0.339
4.81ValArg: 4.81 ± 0.517
4.165ValSer: 4.165 ± 0.585
4.81ValThr: 4.81 ± 0.459
4.106ValVal: 4.106 ± 0.499
1.408ValTrp: 1.408 ± 0.326
1.408ValTyr: 1.408 ± 0.357
0.0ValXaa: 0.0 ± 0.0
Trp
1.056TrpAla: 1.056 ± 0.221
0.176TrpCys: 0.176 ± 0.123
1.466TrpAsp: 1.466 ± 0.276
0.704TrpGlu: 0.704 ± 0.285
0.645TrpPhe: 0.645 ± 0.235
1.232TrpGly: 1.232 ± 0.362
0.352TrpHis: 0.352 ± 0.128
1.115TrpIle: 1.115 ± 0.407
1.232TrpLys: 1.232 ± 0.309
1.701TrpLeu: 1.701 ± 0.36
0.235TrpMet: 0.235 ± 0.118
0.821TrpAsn: 0.821 ± 0.202
0.469TrpPro: 0.469 ± 0.159
1.056TrpGln: 1.056 ± 0.205
1.115TrpArg: 1.115 ± 0.315
1.056TrpSer: 1.056 ± 0.397
1.525TrpThr: 1.525 ± 0.307
1.232TrpVal: 1.232 ± 0.198
0.411TrpTrp: 0.411 ± 0.135
0.821TrpTyr: 0.821 ± 0.267
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.994TyrAla: 1.994 ± 0.45
0.176TyrCys: 0.176 ± 0.116
2.053TyrAsp: 2.053 ± 0.24
2.288TyrGlu: 2.288 ± 0.474
1.056TyrPhe: 1.056 ± 0.308
2.112TyrGly: 2.112 ± 0.424
0.645TyrHis: 0.645 ± 0.213
1.29TyrIle: 1.29 ± 0.202
1.76TyrLys: 1.76 ± 0.38
2.874TyrLeu: 2.874 ± 0.303
0.704TyrMet: 0.704 ± 0.182
1.525TyrAsn: 1.525 ± 0.293
0.997TyrPro: 0.997 ± 0.324
1.584TyrGln: 1.584 ± 0.232
1.877TyrArg: 1.877 ± 0.309
1.936TyrSer: 1.936 ± 0.378
1.584TyrThr: 1.584 ± 0.415
2.874TyrVal: 2.874 ± 0.53
0.528TyrTrp: 0.528 ± 0.174
1.408TyrTyr: 1.408 ± 0.349
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (17049 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski