Mycobacterium phage BirdsNest

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; unclassified Bclasvirinae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9L6V9|A0A6B9L6V9_9CAUD Uncharacterized protein OS=Mycobacterium phage BirdsNest OX=2686231 GN=4 PE=4 SV=1
MM1 pKa = 7.43AQQDD5 pKa = 3.68QPQTYY10 pKa = 9.83LGGVDD15 pKa = 3.42YY16 pKa = 11.36SGIPGSEE23 pKa = 4.08LRR25 pKa = 11.84RR26 pKa = 11.84VIADD30 pKa = 3.56PYY32 pKa = 10.62IPGEE36 pKa = 4.16GASAAEE42 pKa = 4.44LGGEE46 pKa = 4.15

Molecular weight: 4.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LHS5|A0A6B9LHS5_9CAUD Uncharacterized protein OS=Mycobacterium phage BirdsNest OX=2686231 GN=82 PE=4 SV=1
MM1 pKa = 7.8AGRR4 pKa = 11.84GGIARR9 pKa = 11.84DD10 pKa = 3.74TLVTMPTTQRR20 pKa = 11.84PAEE23 pKa = 4.12TRR25 pKa = 11.84PVPTKK30 pKa = 9.98PIQVKK35 pKa = 10.43GMTDD39 pKa = 3.13DD40 pKa = 4.95LIRR43 pKa = 11.84RR44 pKa = 11.84FKK46 pKa = 10.59VAAAEE51 pKa = 4.24EE52 pKa = 4.67GTSYY56 pKa = 11.36AGLIEE61 pKa = 4.07QWLDD65 pKa = 3.13ARR67 pKa = 11.84DD68 pKa = 3.44RR69 pKa = 11.84AAEE72 pKa = 4.0RR73 pKa = 11.84QRR75 pKa = 11.84ARR77 pKa = 11.84QAHH80 pKa = 6.28PLHH83 pKa = 6.97RR84 pKa = 11.84PRR86 pKa = 11.84QEE88 pKa = 3.43ATAA91 pKa = 3.95

Molecular weight: 10.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

22601

42

1975

215.2

23.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.38 ± 0.354

0.947 ± 0.127

6.455 ± 0.291

6.407 ± 0.288

2.358 ± 0.101

9.871 ± 0.515

1.81 ± 0.161

4.606 ± 0.164

3.018 ± 0.149

7.995 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.117

2.597 ± 0.146

6.287 ± 0.218

3.341 ± 0.222

7.093 ± 0.34

4.991 ± 0.156

6.429 ± 0.17

7.243 ± 0.215

1.96 ± 0.14

2.088 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski