Streptomyces sp. SID7803

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1189 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I5F985|A0A6I5F985_9ACTN Cell division protein CrgA OS=Streptomyces sp. SID7803 OX=2690326 GN=crgA PE=3 SV=1
MM1 pKa = 7.5NSPYY5 pKa = 10.61KK6 pKa = 10.43PGTPPCWIDD15 pKa = 4.58LMVPPDD21 pKa = 3.55QQAAIDD27 pKa = 4.39FYY29 pKa = 11.51CDD31 pKa = 3.1LFGWQGEE38 pKa = 4.48VGPPEE43 pKa = 3.74QGGYY47 pKa = 6.87TVCTLKK53 pKa = 11.02GKK55 pKa = 10.22AVAGIMKK62 pKa = 10.34AMNPDD67 pKa = 3.07GTVPDD72 pKa = 4.77PMPPTAWTTYY82 pKa = 10.65LSTDD86 pKa = 4.61DD87 pKa = 3.78IDD89 pKa = 3.83ATVTSVTDD97 pKa = 3.2AGGRR101 pKa = 11.84VVVPAVDD108 pKa = 3.78VMDD111 pKa = 5.11LGRR114 pKa = 11.84MAVISDD120 pKa = 3.51PSGAVFGLWQPGTFDD135 pKa = 2.85GTAIVNEE142 pKa = 4.25HH143 pKa = 6.6GALIWSS149 pKa = 4.16

Molecular weight: 15.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I5FAE4|A0A6I5FAE4_9ACTN Uncharacterized protein (Fragment) OS=Streptomyces sp. SID7803 OX=2690326 GN=GTV15_21205 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.86GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1189

0

1189

138954

21

953

116.9

12.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.379 ± 0.094

1.018 ± 0.037

5.94 ± 0.076

5.703 ± 0.086

2.901 ± 0.06

8.93 ± 0.097

2.402 ± 0.047

3.518 ± 0.057

2.901 ± 0.071

9.372 ± 0.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.047

2.205 ± 0.046

6.088 ± 0.09

3.07 ± 0.053

8.545 ± 0.119

6.142 ± 0.103

6.271 ± 0.081

7.852 ± 0.095

1.475 ± 0.031

2.203 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski