Lactococcus virus CB20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7T2E8|C7T2E8_9CAUD Putative terminase large subunit OS=Lactococcus virus CB20 OX=665887 PE=4 SV=1
MM1 pKa = 6.54KK2 pKa = 10.05QYY4 pKa = 10.55WIIEE8 pKa = 4.05DD9 pKa = 3.85HH10 pKa = 7.12LDD12 pKa = 3.5GGLYY16 pKa = 10.09IMPEE20 pKa = 3.97DD21 pKa = 3.62TSEE24 pKa = 4.61DD25 pKa = 3.56EE26 pKa = 4.37LEE28 pKa = 4.28EE29 pKa = 4.96VEE31 pKa = 5.02DD32 pKa = 4.06TCDD35 pKa = 3.05EE36 pKa = 4.63CGDD39 pKa = 3.69FDD41 pKa = 4.95SVIGKK46 pKa = 8.97FSNWNQLKK54 pKa = 10.51KK55 pKa = 11.07EE56 pKa = 4.03MTDD59 pKa = 3.27VKK61 pKa = 10.6DD62 pKa = 3.12WCPYY66 pKa = 8.58SDD68 pKa = 5.1EE69 pKa = 4.15YY70 pKa = 11.5LKK72 pKa = 11.17SLFEE76 pKa = 4.13

Molecular weight: 9.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7T2H3|C7T2H3_9CAUD Uncharacterized protein OS=Lactococcus virus CB20 OX=665887 PE=4 SV=1
MM1 pKa = 7.46CKK3 pKa = 9.75KK4 pKa = 10.38RR5 pKa = 11.84KK6 pKa = 5.78YY7 pKa = 8.56TKK9 pKa = 9.78MGALYY14 pKa = 10.54SIANAQHH21 pKa = 6.41RR22 pKa = 11.84KK23 pKa = 9.58KK24 pKa = 10.45KK25 pKa = 10.08ADD27 pKa = 4.13KK28 pKa = 9.8IPVRR32 pKa = 11.84AYY34 pKa = 9.48HH35 pKa = 6.61CKK37 pKa = 8.93WCNLYY42 pKa = 10.43HH43 pKa = 7.32LSSQQRR49 pKa = 11.84LNIKK53 pKa = 9.21TGVIGG58 pKa = 3.97

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

8589

36

996

168.4

19.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.275 ± 0.516

0.71 ± 0.179

5.798 ± 0.32

7.021 ± 0.529

4.401 ± 0.316

6.345 ± 0.619

1.211 ± 0.158

7.207 ± 0.281

8.744 ± 0.401

8.895 ± 0.444

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.19

6.695 ± 0.338

2.375 ± 0.253

3.528 ± 0.231

3.307 ± 0.272

6.322 ± 0.502

6.648 ± 0.333

6.275 ± 0.331

1.56 ± 0.168

4.063 ± 0.373
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski