Lentisphaera araneosa HTCC2155
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4982 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A6DGE7|A6DGE7_9BACT N-acetylgalactosamine 6-sulfatase (GALNS) OS=Lentisphaera araneosa HTCC2155 OX=313628 GN=LNTAR_22779 PE=3 SV=1
MM1 pKa = 7.2 YY2 pKa = 9.88 GANGKK7 pKa = 9.59 DD8 pKa = 3.59 DD9 pKa = 3.95 SLHH12 pKa = 6.86 AGLEE16 pKa = 4.32 SPTTYY21 pKa = 10.74 GGAGFTNKK29 pKa = 9.81 SATWTWVGEE38 pKa = 3.9 AAAARR43 pKa = 11.84 VTVEE47 pKa = 4.18 VPSSGIHH54 pKa = 6.74 TIRR57 pKa = 11.84 IWQRR61 pKa = 11.84 EE62 pKa = 4.16 DD63 pKa = 2.76 GTRR66 pKa = 11.84 LDD68 pKa = 4.9 KK69 pKa = 10.98 IIVSNISSYY78 pKa = 10.68 TPDD81 pKa = 4.49 GIGPPEE87 pKa = 4.18 SPMNIISTEE96 pKa = 3.88 SSVDD100 pKa = 3.36 EE101 pKa = 4.02 NSIVSNEE108 pKa = 3.84 NSSDD112 pKa = 3.62 DD113 pKa = 4.45 SSSQSYY119 pKa = 10.31 GSSSILTGTNSIYY132 pKa = 10.86 EE133 pKa = 4.38 DD134 pKa = 4.04 LNSTLLVLNGSNFILEE150 pKa = 4.15 IVTAEE155 pKa = 3.74 GSYY158 pKa = 11.07 LFGCTYY164 pKa = 11.02 GDD166 pKa = 3.68 SFEE169 pKa = 4.34 STIIDD174 pKa = 4.36 DD175 pKa = 3.78 FTFDD179 pKa = 4.28 IIYY182 pKa = 10.1 SDD184 pKa = 3.19 SSEE187 pKa = 3.81 IEE189 pKa = 4.06 EE190 pKa = 5.07 FIHH193 pKa = 7.35 SLFDD197 pKa = 3.58 EE198 pKa = 4.49 NEE200 pKa = 4.05 IIDD203 pKa = 3.96 ILLVEE208 pKa = 4.52
Molecular weight: 22.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.77
IPC_protein 3.745
Toseland 3.554
ProMoST 3.872
Dawson 3.732
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.024
Thurlkill 3.592
EMBOSS 3.656
Sillero 3.872
Patrickios 1.036
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.793
Protein with the highest isoelectric point:
>tr|A6DU75|A6DU75_9BACT N-acetylgalactosamine 6-sulfate sulfatase (GALNS) OS=Lentisphaera araneosa HTCC2155 OX=313628 GN=LNTAR_09921 PE=4 SV=1
MM1 pKa = 7.6 GNLRR5 pKa = 11.84 KK6 pKa = 9.66 KK7 pKa = 10.29 RR8 pKa = 11.84 RR9 pKa = 11.84 SKK11 pKa = 9.72 IAKK14 pKa = 8.79 HH15 pKa = 5.52 KK16 pKa = 10.17 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.31 QSRR22 pKa = 11.84 KK23 pKa = 8.82 NRR25 pKa = 11.84 HH26 pKa = 4.05 KK27 pKa = 9.8 TKK29 pKa = 10.85
Molecular weight: 3.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.067
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.676
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.398
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.038
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4982
0
4982
1741385
20
4990
349.5
39.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.965 ± 0.029
1.178 ± 0.012
5.861 ± 0.031
6.66 ± 0.032
4.597 ± 0.021
6.335 ± 0.036
2.322 ± 0.017
6.646 ± 0.031
7.937 ± 0.037
9.813 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.46 ± 0.017
4.943 ± 0.024
3.934 ± 0.023
3.848 ± 0.02
4.252 ± 0.024
6.974 ± 0.025
4.832 ± 0.029
5.493 ± 0.026
1.332 ± 0.013
3.616 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here