Microlunatus phosphovorus (strain ATCC 700054 / DSM 10555 / JCM 9379 / NBRC 101784 / NCIMB 13414 / VKM Ac-1990 / NM-1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Microlunatus; Microlunatus phosphovorus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5305 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5XIE9|F5XIE9_MICPN VOC domain-containing protein OS=Microlunatus phosphovorus (strain ATCC 700054 / DSM 10555 / JCM 9379 / NBRC 101784 / NCIMB 13414 / VKM Ac-1990 / NM-1) OX=1032480 GN=MLP_28040 PE=4 SV=1
MM1 pKa = 7.81TDD3 pKa = 3.3PSVNLPAAILDD14 pKa = 3.9SLLLDD19 pKa = 3.61TSPYY23 pKa = 10.26LSCDD27 pKa = 2.99EE28 pKa = 5.75CFDD31 pKa = 3.51QLDD34 pKa = 3.71IYY36 pKa = 9.87VEE38 pKa = 4.22RR39 pKa = 11.84LRR41 pKa = 11.84QDD43 pKa = 2.91PTYY46 pKa = 10.55RR47 pKa = 11.84DD48 pKa = 2.99VSMQVHH54 pKa = 6.63LSACAACAEE63 pKa = 4.22EE64 pKa = 5.92AEE66 pKa = 4.39ALMDD70 pKa = 4.49LLSGRR75 pKa = 11.84AGG77 pKa = 3.21

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5XK24|F5XK24_MICPN Uncharacterized protein OS=Microlunatus phosphovorus (strain ATCC 700054 / DSM 10555 / JCM 9379 / NBRC 101784 / NCIMB 13414 / VKM Ac-1990 / NM-1) OX=1032480 GN=MLP_05060 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84VHH17 pKa = 6.33GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84SGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.74GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5305

0

5305

1704599

35

5429

321.3

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.896 ± 0.05

0.752 ± 0.009

6.085 ± 0.031

5.358 ± 0.031

2.794 ± 0.022

8.801 ± 0.035

2.113 ± 0.016

4.211 ± 0.024

2.011 ± 0.026

10.532 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.78 ± 0.012

1.914 ± 0.018

5.739 ± 0.027

3.185 ± 0.02

7.669 ± 0.037

5.738 ± 0.027

6.115 ± 0.027

8.655 ± 0.03

1.582 ± 0.017

2.069 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski