Tolypocladium ophioglossoides (strain CBS 100239) (Elaphocordyceps ophioglossoides)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Ophiocordycipitaceae; Tolypocladium; Tolypocladium ophioglossoides

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9303 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0N2M3|A0A0L0N2M3_TOLOC NAD-dependent histone deacetylase HST3 OS=Tolypocladium ophioglossoides (strain CBS 100239) OX=1163406 GN=TOPH_07130 PE=3 SV=1
MM1 pKa = 7.34AAVAAIVNKK10 pKa = 10.31AVADD14 pKa = 4.11DD15 pKa = 3.87KK16 pKa = 11.6SINLFFNTSKK26 pKa = 10.93AQLGISLQSGTDD38 pKa = 3.13TDD40 pKa = 4.54DD41 pKa = 3.3QANDD45 pKa = 2.76VWATGDD51 pKa = 3.74DD52 pKa = 4.1DD53 pKa = 5.05YY54 pKa = 11.97NGYY57 pKa = 9.45VLNPSSMAGVYY68 pKa = 9.71YY69 pKa = 10.63RR70 pKa = 11.84GLSFVAAVTMPKK82 pKa = 10.0LDD84 pKa = 3.94PNVTQTEE91 pKa = 4.17NQISLVSPVYY101 pKa = 10.55QKK103 pKa = 10.31LTTTTLEE110 pKa = 4.01NNNIALCATPSGNDD124 pKa = 2.49SWLYY128 pKa = 10.28YY129 pKa = 10.64LGG131 pKa = 4.07

Molecular weight: 14.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0NE64|A0A0L0NE64_TOLOC Uncharacterized protein C4G9.04c OS=Tolypocladium ophioglossoides (strain CBS 100239) OX=1163406 GN=TOPH_02678 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.94RR15 pKa = 11.84KK16 pKa = 7.8RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9153

150

9303

4696601

18

17016

504.8

55.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.541 ± 0.025

1.301 ± 0.01

5.886 ± 0.016

5.887 ± 0.024

3.533 ± 0.014

7.202 ± 0.025

2.48 ± 0.011

4.308 ± 0.018

4.517 ± 0.023

8.927 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.009

3.282 ± 0.013

6.289 ± 0.029

4.018 ± 0.018

6.754 ± 0.025

7.982 ± 0.029

5.608 ± 0.016

6.281 ± 0.022

1.429 ± 0.009

2.513 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski