Streptomyces sp. 3214.6

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8041 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5ZHR3|A0A1M5ZHR3_9ACTN SnoaL-like domain-containing protein OS=Streptomyces sp. 3214.6 OX=1882757 GN=SAMN05444521_5954 PE=4 SV=1
MM1 pKa = 7.54SGACGIGPSTSAYY14 pKa = 10.46AAGCTGGVDD23 pKa = 3.85SDD25 pKa = 4.32FNGDD29 pKa = 3.7GVRR32 pKa = 11.84DD33 pKa = 3.61TAIADD38 pKa = 3.63PQATVSGQEE47 pKa = 3.58AAGAIQIVLGGGKK60 pKa = 10.19GVTTLTQDD68 pKa = 3.42TPNVSDD74 pKa = 3.55VAEE77 pKa = 4.88AGDD80 pKa = 3.56QFGFSLAVYY89 pKa = 9.43DD90 pKa = 5.24ANLDD94 pKa = 3.7GCSDD98 pKa = 3.52IAVGMPYY105 pKa = 10.48EE106 pKa = 4.63DD107 pKa = 3.62VGSVQDD113 pKa = 3.74AGLVQLIYY121 pKa = 10.92GSTAGLGQGTTGSLGFRR138 pKa = 11.84QGADD142 pKa = 2.81GNMADD147 pKa = 4.1VYY149 pKa = 10.69EE150 pKa = 5.29AEE152 pKa = 4.28DD153 pKa = 3.43WVGYY157 pKa = 9.75SVAGGVSATGVPFLVIGVPGEE178 pKa = 4.26DD179 pKa = 3.38SSGLTDD185 pKa = 3.72MGLAAYY191 pKa = 9.5VAGVTPAVTSFHH203 pKa = 6.33QNSPDD208 pKa = 3.27VWHH211 pKa = 7.44DD212 pKa = 3.21AAAGNRR218 pKa = 11.84FGWAVAATGRR228 pKa = 11.84HH229 pKa = 4.78FVVGAPGAAIDD240 pKa = 4.63AADD243 pKa = 3.4QAGAVVAFKK252 pKa = 10.71PSLNADD258 pKa = 4.61NIPQPLFGIGQDD270 pKa = 3.32ATGDD274 pKa = 3.64KK275 pKa = 11.0DD276 pKa = 3.59PTAEE280 pKa = 4.11TGDD283 pKa = 3.61GFGISVAIAPYY294 pKa = 9.76RR295 pKa = 11.84PSGATATTDD304 pKa = 3.13SLVAVGVPNEE314 pKa = 4.18DD315 pKa = 3.03VGTKK319 pKa = 9.13IDD321 pKa = 3.45AGGVQLLQIAEE332 pKa = 4.35AGTVTEE338 pKa = 4.68TQWITQDD345 pKa = 3.27TADD348 pKa = 3.41VDD350 pKa = 4.1EE351 pKa = 4.63TAEE354 pKa = 3.89EE355 pKa = 3.97SDD357 pKa = 3.76YY358 pKa = 11.3FGQRR362 pKa = 11.84VAAANTNQGVVGTATTMRR380 pKa = 11.84VAVGVPGEE388 pKa = 4.12EE389 pKa = 3.7TAAAAQDD396 pKa = 3.51AGGVQIFPMLGAAGTSDD413 pKa = 3.45SWLQPGSGIPAAAAPRR429 pKa = 11.84QFAGMSVGATGSLLYY444 pKa = 10.72VGLPYY449 pKa = 10.83GPAAGRR455 pKa = 11.84AVYY458 pKa = 9.98GYY460 pKa = 10.1AWTVASGGAPSQTWKK475 pKa = 10.0PGEE478 pKa = 4.14GGIPAGAGAFGATVRR493 pKa = 4.25

Molecular weight: 48.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5YTB1|A0A1M5YTB1_9ACTN Putative peptidoglycan lipid II flippase OS=Streptomyces sp. 3214.6 OX=1882757 GN=SAMN05444521_4515 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.89GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8041

0

8041

2692240

29

5005

334.8

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.451 ± 0.04

0.799 ± 0.008

5.993 ± 0.022

5.567 ± 0.03

2.721 ± 0.016

9.366 ± 0.027

2.302 ± 0.015

3.144 ± 0.019

2.283 ± 0.025

10.174 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.7 ± 0.011

1.893 ± 0.017

6.081 ± 0.028

2.906 ± 0.017

7.836 ± 0.032

5.242 ± 0.023

6.382 ± 0.029

8.432 ± 0.026

1.562 ± 0.013

2.163 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski