Proteome-pI 2.0 - Proteome Isoelectric Point Database

Cotton leaf curl Burewala betasatellite

Taxonomy: Viruses; Tolecusatellitidae; Betasatellite; unclassified Betasatellite

Average proteome isoelectric point is 4.85

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|D3HTY4|D3HTY4_9VIRU Beta C1 OS=Cotton leaf curl Burewala betasatellite OX=713092 GN=beta C1 PE=4 SV=1

MM1 pKa = 6.54TTSGTNKK8 pKa = 9.31EE9 pKa = 4.16GVRR12 pKa = 11.84FIVDD16 pKa = 3.01VRR18 pKa = 11.84IMEE21 pKa = 3.95NMKK24 pKa = 10.18IFIHH28 pKa = 5.63MRR30 pKa = 11.84ILSTKK35 pKa = 9.42SPSLIKK41 pKa = 10.8YY42 pKa = 8.84EE43 pKa = 4.8GIVQYY48 pKa = 9.82TYY50 pKa = 11.58EE51 pKa = 5.23DD52 pKa = 2.88IHH54 pKa = 8.78VPFDD58 pKa = 3.91FNGFEE63 pKa = 4.26GSIIANFLFAYY74 pKa = 9.97NGAKK78 pKa = 9.52IEE80 pKa = 4.34EE81 pKa = 4.37IEE83 pKa = 4.54IEE85 pKa = 4.62DD86 pKa = 3.45IVHH89 pKa = 6.8RR90 pKa = 11.84LDD92 pKa = 4.06ILVLEE97 pKa = 4.49NPEE100 pKa = 4.0ILGMDD105 pKa = 3.96VIEE108 pKa = 4.71PYY110 pKa = 10.42IFNKK114 pKa = 10.0KK115 pKa = 7.85FTVV118 pKa = 3.26

Molecular weight: 13.68 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|D3HTY4|D3HTY4_9VIRU Beta C1 OS=Cotton leaf curl Burewala betasatellite OX=713092 GN=beta C1 PE=4 SV=1

MM1 pKa = 6.54TTSGTNKK8 pKa = 9.31EE9 pKa = 4.16GVRR12 pKa = 11.84FIVDD16 pKa = 3.01VRR18 pKa = 11.84IMEE21 pKa = 3.95NMKK24 pKa = 10.18IFIHH28 pKa = 5.63MRR30 pKa = 11.84ILSTKK35 pKa = 9.42SPSLIKK41 pKa = 10.8YY42 pKa = 8.84EE43 pKa = 4.8GIVQYY48 pKa = 9.82TYY50 pKa = 11.58EE51 pKa = 5.23DD52 pKa = 2.88IHH54 pKa = 8.78VPFDD58 pKa = 3.91FNGFEE63 pKa = 4.26GSIIANFLFAYY74 pKa = 9.97NGAKK78 pKa = 9.52IEE80 pKa = 4.34EE81 pKa = 4.37IEE83 pKa = 4.54IEE85 pKa = 4.62DD86 pKa = 3.45IVHH89 pKa = 6.8RR90 pKa = 11.84LDD92 pKa = 4.06ILVLEE97 pKa = 4.49NPEE100 pKa = 4.0ILGMDD105 pKa = 3.96VIEE108 pKa = 4.71PYY110 pKa = 10.42IFNKK114 pKa = 10.0KK115 pKa = 7.85FTVV118 pKa = 3.26

Molecular weight: 13.68 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	118	118	118	118.0	13.68

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
2.542 ± 0.0	0.0 ± 0.0	5.085 ± 0.0	10.169 ± 0.0	7.627 ± 0.0	5.932 ± 0.0	2.542 ± 0.0	15.254 ± 0.0	5.932 ± 0.0	5.932 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
4.237 ± 0.0	5.932 ± 0.0	3.39 ± 0.0	0.847 ± 0.0	3.39 ± 0.0	4.237 ± 0.0	5.085 ± 0.0	7.627 ± 0.0	0.0 ± 0.0	4.237 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski